检索结果-内蒙古大学图书馆

TISSUES 2.0: an integrative web resource on mammalian tissue expression (vol 2018, 2018)

DATABASE-THE JOUrnaL OF BIOLOGICAL DATABASES AND CURATION 2018年第2018期2018卷 bay028页

作者： Palasca, Oana Santos, Alberto Stolte, Christian Gorodkin, Jan Jensen, Lars Juhl Novo Nordisk Foundation Center for Protein Research Faculty of Health and Medical Sciences University of Copenhagen Copenhagen Denmark. Center for non-coding RNA in Technology and Health Faculty of Health and Medical Sciences University of Copenhagen Copenhagen Denmark. Department of Veterinary and Animal Sciences Faculty of Health and Medical Sciences University of Copenhagen Copenhagen Denmark. New York Genome Center New York NY USA.

Physiological and molecular similarities between organisms make it possible to translate findings from simpler experimental systems—model organisms—into more complex ones, such as human. This translation facilitates the understanding of biological processes under normal or disease conditions. Researchers aiming to identify the similarities and differences between organisms at the molecular level need resources collecting multi-organism tissue expression data. We have developed a database of gene–tissue associations in human, mouse, rat and pig by integrating multiple sources of evidence: transcriptomics covering all four species and proteomics (human only), manually curated and mined from the scientific literature. Through a scoring scheme, these associations are made comparable across all sources of evidence and across organisms. Furthermore, the scoring produces a confidence score assigned to each of the associations. The TISSUES database (version 2.0) is publicly accessible through a user-friendly web interface and as part of the STRING app for Cytoscape. In addition, we analyzed the agreement between datasets, across and within organisms, and identified that the agreement is mainly affected by the quality of the datasets rather than by the technologies used or organisms compared.;http://***/

关键词：

来源：评论

学校读者我要写书评

暂无评论

BlockClust: Efficient clustering and classification of non-coding rnas from short read rna-Seq profiles

BlockClust: Efficient clustering and classification of non-c...

引用

International Conference on German Conference on Bioinformatics, GCB 2014

作者： Videm, Pavankumar Rose, Dominic Costa, Fabrizio Backofen, Rolf Bioinformatics Group Department of Computer Science Albert-Ludwigs-University Freiburg Georges-Köhler-Allee 106 FreiburgD-79110 Germany Munich Germany Albert-Ludwigs-University Freiburg FreiburgD-79104 Germany Albert-Ludwigs-University Freiburg Habsburgerstr. 49 FreiburgD-79104 Germany Centre for Non-Coding RNA in Technology and Health University of Copenhagen Gronnegardsvej 3 Frederiksberg CDK-1870 Denmark

ISBN: (纸本)9783885796299

Sequence and secondary structure analysis can be used to assign putative functions to non-coding rnas. However sequence information is changed by post-transcriptional modifications and secondary structure is only a proxy for the true 3D conformation of the rna polymer. In order to tackle these issues we can extract a different type of description using the pattern of processing that can be observed through the traces left in small rna-seq reads data. To obtain an efficient and scalable procedure, we propose to encode expression profiles in discrete structures, and process them using fast graph-kernel techniques. We present BlockClust for both clustering and classification of small non-coding rna transcripts with similar processing patterns. We show how the proposed approach is scalable, accurate and robust across different organisms, tissues and cell lines. BlockClust was successfully applied on a comprehensive set of eukaryotic data. It is the first tool for eukaryotic non-coding rna analysis available on the galaxy framework.

关键词： Cell culture

来源：评论

学校读者我要写书评

暂无评论

Single-cell rna sequencing-based immunological feature analysis of a COVID-19 patient with recurrent positive SARS-CoV-2 rna

引用

Chinese Medical Journal 2022年第8期135卷 1000-1002页

作者： Bin Su Ruya Sun Lili Zhang Jiaying Zhang Xia Feng Zhenhuan Cao Wei Hou Xianghua Guo Qing Ge Qinghua Cui Yuan Zhou Yingmin Ma Ronghua Jin Yingmei Feng Beijing Youan Hospital Capital Medical UniversityBeijing 100069China Department of Biomedical Informatics School of Basic Medical SciencesKey Laboratory of Molecular Cardiovascular Sciences of the Ministry of EducationCenter for Non-coding RNA MedicinePeking University Health Science CenterBeijing 100191China Department of Immunology Key Laboratory of Medical ImmunologyMinistry of HealthSchool of Basic Medical SciencesPeking University Health Science CenterBeijing 100191China Beijing Ditan Hospital Capital Medical UniversityBeijing 100102China

To the Editor:Since the outbreak in December 2019,severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has infected>2.4 billion people worldwide,resulting in>4.9 million *** understand the pathogenesis of SARS-CoV-2 infection,single-cell rna-sequencing(scrna-seq)profiles of peripheral blood mononuclear cells(PBMCs)have been constructed in coronavirus disease-2019(COVID-19)patients at different disease stages.

关键词： acute respiratory pathogenesis

来源：评论

学校读者我要写书评

暂无评论

Ribosomal modification protein rimK-like family member A activates betaine-homocysteine S-methyltransferase 1 to ameliorate hepatic steatosis

引用

Signal Transduction and Targeted Therapy 2024年第9期9卷 4048-4065页

作者： Han Yan Wenjun Liu Rui Xiang Xin Li Song Hou Luzheng Xu Lin Wang Dong Zhao Xingkai Liu Guoqing Wang Yujing Chi Jichun Yang Department of Physiology and Pathophysiology School of Basic Medical SciencesState Key Laboratory of Vascular Homeostasis and RemodelingCenter for Non-coding RNA MedicinePeking University Health Science CenterBeijing 100191China Department of Endocrinology The Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310058China Medical and Health Analysis Center Peking UniversityBeijing 100191China Department of Hepatobiliary Surgery Xi-Jing HospitalFourth Military Medical UniversityXi’an 710032China Department of Endocrinology Beijing Luhe HospitalCapital Medical UniversityBeijing 101100China Department of Hepatobiliary and Pancreatic Surgery General Surgery CentreFirst Hospital of Jilin UniversityChangchun 130061China Key Laboratory of Pathobiology Ministry of Education College of Basic Medical SciencesJilin UniversityChangchun 130012China Department of Central Laboratory and Institute of Clinical Molecular Biology Department of GastroenterologyPeking University People’s HospitalBeijing 100044China Department of Cardiology Peking University Third HospitalBeijing 100191China

nonalcoholic fatty liver disease(NAFLD)is a serious threat to public health,but its underlying mechanism remains poorly *** screening important genes using Gene Importance Calculator(GIC)we developed previously,ribosomal modification protein rimK-like family member A(RIMKLA)was predicted as one essential gene but its functions remained largely *** current study determined the roles of RIMKLA in regulating glucose and lipid *** expression was reduced in livers of human and mouse with *** RIMKLA overexpression ameliorated steatosis and hyperglycemia in obese ***-specific RIMKLA knockout aggravated high-fat diet(HFD)-induced dysregulated glucose/lipid metabolism in ***,RIMKLA is a new protein kinase that phosphorylates betaine-homocysteine S-methyltransferase 1(BHMT1)at threonine 45(Thr45)*** phosphorylation at Thr45 and activation,BHMT1 eliminated homocysteine(Hcy)to inhibit the activity of transcription factor activator protein 1(AP1)and its induction on fatty acid synthase(FASn)and cluster of differentiation 36(CD36)gene transcriptions,concurrently repressing lipid synthesis and uptake in ***45 to alanine(T45A)mutation inactivated BHMT1 to abolish RIMKLA’s repression on Hcy level,AP1 activity,FASn/CD36 expressions,and lipid ***1 overexpression rescued the dysregulated lipid metabolism in RIMKLA-deficient *** summary,RIMKLA is a novel protein kinase that phosphorylates BHMT1 at Thr45 to repress lipid synthesis and *** obese condition,inhibition of RIMKLA impairs BHMT1 activity to promote hepatic lipid deposition.

关键词： metabolism hepatic homocysteine

来源：评论

学校读者我要写书评

暂无评论

A Retrospective Analysis for Different Routes of Administration in Mice-Percutaneous Retro-Orbital, Jugular Catheter, Tail Vein and Femoral Cut Down Injections

引用

Journal of Biosciences and Medicines 2020年第9期8卷 131-141页

作者： Priyanka Prathipati Cristian Rodriguez-Aguayo Brian L Walton Anil K. Sood Jamieson Greaver Christopher F. Janssen Gabriel Lopez-Berestein Department of Advanced Cardiopulmonary Therapies and Transplantation Center for Advanced Heart Failure University of Texas Health Science Center at Houston Houston TX USA Department of Experimental Therapeutics University of Texas M.D. Anderson Cancer Center Houston TX USA The Center for RNA Interference and Non-Coding RNA University of Texas M.D. Anderson Cancer Center Houston TX USA Department of Cancer Biology University of Texas M.D. Anderson Cancer Center Houston TX USA Department of Gynecology Oncology & Reproductive Medicine University of Texas M.D. Anderson Cancer Center Houston TX USA Interdisciplinary Research Center for Laboratory Animal Medicine and Care University of Texas Health Science Center at Houston Houston TX USA

Liposomes effectively transport fatty proteins to targeted tissues. Laboratory experiments use multiple methods to administer liposomes, but comparison of these methods is not available. In this retrospective study, we characterized and compared four intravenous administration routes (tail vein, jugular catheter, femoral vein and percutaneous retro-orbital injections) in murine models. ApoE^-/- mice were used to compare administration routes. Results indicate that the jugular catheter route delivered the highest amount of liposomes to tissues due to longer period of injections compared to other routes;however, this route failed to remain patent for 8/10 animals. Delivery via tail vein, femoral vein and percutaneous retro-orbital injections resulted in similar accumulation in the organs. When including technical difficulty and expense, percutaneous retro-orbital injections of liposomes are the most convenient and efficacious approach.

关键词： Femoral Vein Jugular Catheter Liposomes Retro-Orbital Tail Vein

来源：评论

学校读者我要写书评

暂无评论

Fatty Acid Binding Protein-4 Silencing Attenuates Atherosclerosis Progression by Affecting Macrophage Apoptosis and Autophagy

引用

Journal of Biosciences and Medicines 2019年第7期7卷 99-109页

作者： Siqin Zhaorigetu Priyanka Prathipati Cristian Rodriguez-Aguayo Brian L. Walton Anil K. Sood Gabriel Berestein-Lopez Department of Pediatric Surgery Center for Advanced Heart Failure University of Texas Health Science Center at Houston Houston TX USA Department of Advanced Cardiopulmonary Therapies and Transplantation Center for Advanced Heart Failure University of Texas Health Science Center at Houston Houston TX USA Department of Experimental Therapeutics The University of Texas M.D. Anderson Cancer Center Houston TX USA The Center for RNA Interference and Non-Coding RNA The University of Texas M.D. Anderson Cancer Center Houston TX USA Department of Cancer Biology The University of Texas M.D. Anderson Cancer Center Houston TX USA Department of Gynecology Oncology & Reproductive Medicine The University of Texas M.D. Anderson Cancer Center Houston TX USA

Background: Atherosclerosis, which is the principal cause of heart attacks and strokes, is the leading cause of death in the United States. Various biological processes such as apoptosis and autophagy are involved in almost every stage of atherosclerosis, which could lead to plaque instability causing stroke and death. Fatty Acid Binding Protein 4 (FABP4) is an adipokine released by macrophages and is involved in multiple disease conditions including stroke. However, the association of FABP4 with macrophage apoptosis and autophagy in atherosclerosis has not been elucidated. We hypothesize that silencing FABP4 protein could be a novel therapeutic approach to attenuate macrophage apoptosis and autophagy thereby minimizing plaque instability in atherosclerosis. Methods: RAW264 mouse macrophage cells were transfected with sirna control liposome quantum dots (QD), siFABP4 liposome QD at the concentration of 150 μg/ml total lipids, or TNF-α at 100 ng/ml. Western blot and reverse phase protein array (RPPA) analysis were completed. Results: Inhibiting the translation of FABP4 blocked the apoptotic pathway as evidenced from the increased expression of anti-apoptotic BCL-xL and BCL-2 along with reduced expression of BAX and activated Caspase 3 levels. Beclin-1 and LC3-II levels were also reduced with knocking down FABP4 indicating the attenuation of autophagy. Conclusion: Targeting FABP4 protects against macrophage processes associated with the progression of atherosclerosis.

关键词： Atherosclerosis Apoptosis Liposomes Macrophages FABP4

来源：评论

学校读者我要写书评

暂无评论

Maximizing output and recognizing autocatalysis in chemical reaction networks is NP-complete

引用

Journal of Systems Chemistry 2012年第1期3卷 1-9页

作者： Andersen, Jakob L. Flamm, Christoph Merkle, Daniel Stadler, Peter F. Department for Mathematics and Computer Science University of Southern Denmark DK-5230 Odense M Campusvej 55 Denmark Institute for Theoretical Chemistry University of Vienna A-1090 Wien Währingerstraße 17 Austria Bioinformatics Group Department of Computer Science Interdisciplinary Center for Bioinformatics D-04107 Leipzig Härtelstraße 16-18 Germany Max Planck Institute for Mathematics in the Sciences D-04103 Leipzig Inselstraße 22 Germany Fraunhofer Institute for Cell Therapy and Immunology D-04103 Leipzig Perlickstraße 1 Germany Center for non-coding RNA in Technology and Health University of Copenhagen DK-1870 Frederiksberg C Grønnegårdsvej 3 Denmark Santa Fe Institute Santa Fe NM 87501 1399 Hyde Park Rd. United States

Background: A classical problem in metabolic design is to maximize the production of a desired compound in a given chemical reaction network by appropriately directing the mass flow through the network. Computationally, this problem is addressed as a linear optimization problem over the flux cone. The prior construction of the flux cone is computationally expensive and no polynomial-time algorithms are known. Results: Here we show that the output maximization problem in chemical reaction networks is NP-complete. This statement remains true even if all reactions are monomolecular or bi-molecular and if only a single molecular species is used as influx. As a corollary we show, furthermore, that the detection of autocatalytic species, i.e., types that can only be produced from the influx material when they are present in the initial reaction mixture, is an NPcomplete computational problem. Conclusions: Hardness results on combinatorial problems and optimization problems are important to guide the development of computational tools for the analysis of metabolic networks in particular and chemical reaction networks in general. Our results indicate that efficient heuristics and approximate algorithms need to be employed for the analysis of large chemical networks since even conceptually simple flow problems are provably intractable. © 2012 Andersen et al.;licensee Chemistry Central Ltd.

关键词：

来源：评论

学校读者我要写书评

暂无评论

Microrna or not microrna?

MicroRNA or not microRNA?

引用

Lecture Notes in Computer Science

作者： Langenberger, David Bartschat, Sebastian Hertel, Jana Hoffmann, Steve Tafer, Hakim Stadler, Peter F. Bioinformatics Group Department of Computer Science University of Leipzig D-04107 Leipzig United Kingdom Interdisciplinary Center for Bioinformatics University of Leipzig Härtelstraße 16-18 D-04107 Leipzig Germany LIFE - Leipzig Research Center for Civilization Diseases University of Leipzig Germany Max Planck Institute for Mathematics in the Sciences Inselstraße 22 D-04103 Leipzig Germany Fraunhofer Institut für Zelltherapie und Immunologie Perlickstraße 1 D-04103 Leipzig Germany Department of Theoretical Chemistry University of Vienna Währingerstraße 17 A-1090 Wien Austria Center for Non-coding RNA in Technology and Health University of Copenhagen Grønnegårdsvej 3 DK-1870 Frederiksberg C Denmark Santa Fe Institute 1399 Hyde Park Rd. Santa Fe NM 87501 United States

ISBN: (纸本)9783642228247

The avalanche of next generation sequencing data has led to a rapid increase of annotated micrornas in the last few years. Many of them are specific to individual species or rather narrow clades. A closer inspection of the current version of miRBase shows that dozens of entries conflict with other ncrnas, in particular snornas. With few exceptions, these cases show little similarities to canonical micrornas, however, and thus they should be considered as mis-annotations. © 2011 Springer-Verlag.

关键词： rna

来源：评论

学校读者我要写书评

暂无评论

Diagnostic and Therapeutic Micrornas in Primary Myelofibrosis

引用

Proceedings of the Singapore National Academy of Science 2020年第2期14卷 91-109页

作者： Roxana Manaila Vlad Moisoiu Erik Knutsen Mihnea P. Dragomir George A. Calin Clinical Institute of Urology and Renal Transplantation Cluj-Napoca Romania Contributed equally to this manuscript. Faculty of Physics Babeş-Bolyai University Cluj-Napoca Romania Department of Medical Biology Faculty of Health Sciences UiT — The Arctic University of Norway Tromsø Norway Department of Surgery Fundeni Clinical Hospital Carol Davila University of Medicine and Pharmacy Bucharest Romania Department of Experimental Therapeutics The University of Texas MD Anderson Cancer Center Houston TX USA The Center for RNA Interference and Non-Coding RNAs The University of Texas MD Anderson Cancer Center Houston TX USA

Primary myelofibrosis (PMF) is a pluripotent hematopoietic stem cell-derived malignancy, included in the heterogeneous group of myeloproliferative neoplasms (MPNs). PMF diagnosis is based on a composite assessment of clinical and laboratory data. The three major diagnostic criteria are: screening for driver mutations, exclusion of other conditions that can cause myelofibrosis, and bone marrow biopsy displaying megakaryocyte changes and fibrosis. PMF treatment options are only partially disease-modifying and consist mainly of symptom control. Recently, a new targeted therapy was introduced for PMF patients, JAK-STAT inhibitors (i.e. ruxolitinib). However, specific subgroups of patients do not benefit from the JAK-STAT inhibitors: (1) those who are carrying JAK2 mutations, but ruxolitinib does not reduce the spleen size; (2) triple negative patients (no JAK2 , CALR , or MPL mutations); and (3) those who discontinue JAK-STAT therapy because of side effects. These subgroups are in need of new therapeutic approaches. Mature micrornas (mirnas) range from 16 to 28 nucleotides (nt) in length and regulate specific messenger rnas at the post-transcriptional level. Numerous in vitro and in vivo studies have reported specific mirnas, as well as complex mirna networks, to be dysregulated in PMF. Several of these mirnas were shown to be implicated in essential events of PMF pathophysiology: increase of bone marrow fibrosis, progression to acute myeloid leukemia, resistance to JAK-STAT inhibitors, and activation of differentiation of hematopoietic stem/progenitor cells into megakaryocytes. Hence, we propose mirnas as a potential minimally invasive diagnostic tool for PMF and as therapeutic targets that could address the unmet medical needs of these patients.

关键词： Primary myelofibrosis myeloproliferative neoplasms microrna network theory JAK-STAT inhibitors ruxolitinib miR-379/656 cluster microrna therapy

来源：评论

学校读者我要写书评

暂无评论

Association between nonalcoholic Fatty Liver Disease and Carotid Artery Disease in a Community-Based Chinese Population： A Cross-Sectional Study

引用

Chinese Medical Journal 2018年第19期131卷 2269-2276页

作者： Yu-Chen Guo Yong Zhou Xing Gao Yan Yao Bin Geng Qing-Hua Cui Ji-Chun Yang Hong-Pu Hu Department of Health Information Management Institute of Medical Information Peking Union Medical College & Chinese Academy of Medical Sciences Beijing 100020 China Beijing Institute of Heart Lung and Blood Vessel Diseases Beijing Anzhen Hospital Capital Medical University Beijing 100029 China Department of Cardiology Beijing Anzhen Hospital Capital Medical University Beijing 100029 China Hypertension Center Fuwai Hospital Chinese Academy of Medical Sciences and Peking Union Medical College State Key Laboratory of Cardiovascular Disease Beijing 100037 China Department of Biomedical Informatics School of Basic Medical Sciences Key Laboratory of Molecular Cardiovascular Science of the Ministry of Education Center for Non-coding RNA Medicine Peking University Health Science Center Beijing 100191 China Department of Physiology and Pathophysiology School of Basic Medical Sciences Peking University Health Science Center Beijing 100191 China

Background： nonalcoholic fatty liver disease （NAFLD） is one of the most common chronic liver diseases with a high prevalence in the general population. The association between NAFLD and cardiovascular disease has been well addressed in previous studies. However, whether NAFLD is associated with carotid artery disease in a community-based Chinese population remained unclear. The aim of this study was to investigate the association between NAFLD and carotid artery disease. Methods： A total of 2612 participants （1091 men and 1521 women） aged 40 years and older from Jidong of Tangshan city （China） were selected for this study. NAFLD was diagnosed by abdominal ultrasonography. The presence of carotid stenosis or plaque was evaluated by carotid artery ultrasonography. Logistic regression was used to analyze the association between NAFLD and carotid artery disease. Results： Participants with NAFLD have a higher prevalence of carotid stenosis （12.9% vs. 4.6%） and carotid plaque （21.9% vs. 15.0%） than those without NAFLD. After adjusting for age, gender, smoking status, income, physical activity, diabetes, hypertension, triglyceride, waist-hip ratio, and high-density lipoprotein, NAFLD is significantly associated with carotid stenosis （odds ratio [OR]： 2.06, 95% confidence interval [CI]： 1.45-2.91）, but the association between NAFLD and carotid plaque is not statistically significant （OR： 1. 10, 95% CI： 0.86-1.40）. Conclusion： A significant association between NAFLD and carotid stenosis is found in a Chinese population.

关键词： Association Carotid Artery Disease Carotid Stenosis nonalcoholic Fatty Liver Disease

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：