检索结果-内蒙古大学图书馆

Profiling of Ribose Methylations in rna by High‐Throughput Sequencing†

Angewandte Chemie 2014年第2期127卷

作者： Ulf Birkedal Mikkel Christensen‐Dalsgaard Nicolai Krogh Radhakrishnan Sabarinathan Jan Gorodkin Henrik Nielsen Department of Cellular and Molecular Medicine University of Copenhagen Blegdamsvej 3B 2200 Copenhagen N (Denmark) Center for Non‐coding RNA in Technology and Health Department of Veterinary Clinical and Animal Science University of Copenhagen Grønnegårdsvej 3 1870 Frederiksberg C (Denmark)

Ribose methylations are the most abundant chemical modifications of ribosomal rna and are critical for ribosome assembly and fidelity of translation. Many aspects of ribose methylations have been difficult to study due to lack of efficient mapping methods. Here, we present a sequencing‐based method (RiboMeth‐seq) and its application to yeast ribosomes, presently the best‐studied eukaryotic model system. We demonstrate detection of the known as well as new modifications, reveal partial modifications and unexpected communication between modification events, and determine the order of modification at several sites during ribosome biogenesis. Surprisingly, the method also provides information on a subset of other modifications. Hence, RiboMeth‐seq enables a detailed evaluation of the importance of rna modifications in the cells most sophisticated molecular machine. RiboMeth‐seq can be adapted to other rna classes, for example, mrna, to reveal new biology involving rna modifications.

关键词： Ribosome Ribozyme rna‐Erkennung rna‐Strukturen Transferasen

来源：评论

学校读者我要写书评

暂无评论

Discovery of a molecular glue that enhances UPRmt to restore proteostasis via TRKA-GRB2-EVI1-CRLS1 axis

Discovery of a molecular glue that enhances UPRmt to restore...

引用

2022生物物理大会

作者： Li-Feng-Rong Qi Shuai Liu Qiuyuan Fang Cheng Qian Chao Peng Yuci Liu Peng Yang Ping Wu Ling Shan Qinghua Cui Liangyi Chen Wei Yang Ping Li Xiaojun Xu State Key Laboratory of Natural Medicines China Pharmaceutical University Department of Biophysics and Department of Neurosurgery of the First Affiliated Hospital Zhejiang University School of Medicine National Facility for Protein Science in Shanghai Zhangjiang Lab Shanghai Advanced Research Institute Chinese Academy of Science Shanghai Science Research Center Chinese Academy of Sciences Dept.Neuropsychiatric Disorders Netherlands Institute for Neuroscience An Institute of the Royal Netherlands Academy of Arts and Sciences Department of Biomedical Informatics School of Basic Medical Sciences Key Laboratory of Molecular Cardiovascular Sciences of the Ministry of Education Center for Non-Coding RNA Medicine Peking University Health Science Center Beijing PKU-IDG/McGovern Institute for Brain Research

Lowering proteotoxicity is a potentially powerful approach for the treatment of neurological disorders, such as Parkinson's disease. The unfolded protein response(UPR) is a major mechanism that preserves the network maintaining cellular proteostasis. In the present study, we developed the screening strategy to discover compounds that significantly enhanced the activation of mitochondrial UPR(UPRmt)through increasing cardiolipin content. We identified that ginsenoside Rg3(Rg3)increased cardiolipin depending on cardiolipin synthase 1(CRLS1) in both worms and in human neural cells. Using LiP-SMap(limited proteolysis-mass spectrometry)strategy, we identified GRB2(growth factor receptor bound protein 2) as a direct target of Rg3 in human neural cells. Rg3 enhances the binding between GRB2 and TRKA(tropomyosin-related kinase A), that transduces signals via phosphrorylation of ERK(extracellular signal-regulated kinase). We provided bioinformatic and experimental evidences showing that EVI1(ecotropic virus integration site-1), the critical oncogenic transcriptional regulator in leukemia, binds to CRLS1 promoter region and stimulated CRLS1 expression and subsequently increased cardiolipin content in the presence of Rg3. Rg3 stimulates alpha-synuclein clearance by promoting mitochondrial peripheral fission and mitophagy. In two Parkinson's disease mouse models, Rg3 restores motor function by protecting nigral dopaminergic neurons dependent on GRB2. Consistently, Rg3 protected iPSC-derived dopaminergic neurons by restoring ERK/CRLS1 signaling and mitochondrial function. Our data recapitulate the TRKA-GRB2-EVI1-CRLS1 axis in maintaining proteostasis in Parkinson's disease via restoring mitochondrial homeostasis.

关键词： Parkinson's disease cardiolipin UPRmt Ginsenoside Rg3 GRB2 TRKA EVI1

来源：评论

学校读者我要写书评

暂无评论

TP53 loss creates therapeutic vulnerability in colorectal cancer (vol 30, pg 697, 2015)

引用

NATURE 2021年第7875期597卷 E6-E6页

作者： Liu, Yunhua Zhang, Xinna Han, Cecil Wan, Guohui Huang, Xingxu Ivan, Cristina Jiang, Dahai Rodriguez-Aguayo, Cristian Lopez-Berestein, Gabriel Rao, Pulivarthi H. Maru, Dipen M. Pahl, Andreas He, Xiaoming Sood, Anil K. Ellis, Lee M. Anderl, Jan Lu, Xiongbin Department of Cancer Biology The University of Texas MD Anderson Cancer Center Houston TX USA Department of Gynaecologic Oncology and Reproductive Medicine The University of Texas MD Anderson Cancer Center Houston USA Center for RNA Interference and Non-coding RNAs The University of Texas MD Anderson Cancer Center Houston TX USA Department of Experimental Therapeutics The University of Texas MD Anderson Cancer Center Houston TX USA School of Life Science and Technology ShanghaiTech University Shanghai China Department of Paediatrics Baylor College of Medicine Houston TX USA Department of Pathology The University of Texas MD Anderson Cancer Center Houston TX USA Heidelberg Pharma GmbH Ladenburg Germany Department of Biomedical Engineering The Ohio State University Columbus OH USA Department of Surgical Oncology The University of Texas MD Anderson Cancer Center Houston TX USA

来源：评论

学校读者我要写书评

暂无评论

The Theory of Gene Family Histories

arXiv

引用

arXiv 2023年

作者： Hellmuth, Marc Stadler, Peter F. Department of Mathematics Faculty of Science Stockholm University StockholmSE - 106 91 Sweden Bioinformatics Group Department of Computer Science Interdisciplinary Center for Bioinformatics University of Leipzig Härtelstraße 16-18 LeipzigD-04107 Germany Max Planck Institute for Mathematics in the Sciences Inselstraße 22 LeipzigD-04103 Germany Fraunhofer Institute for Cell Therapy and Immunology Perlickstraße 1 LeipzigD-04103 Germany Institute for Theoretical Chemistry University of Vienna Währingerstraße 17 WienA-1090 Austria Center for Non-coding RNA in Technology and Health University of Copenhagen Grønnegårdsvej 3 Frederiksberg CDK-1870 Denmark Santa Fe Institute 1399 Hyde Park Rd Santa FeNM87501 United States

Most genes are part of larger families of evolutionary related genes. The history of gene families typically involves duplications and losses of genes as well as horizontal transfers into other organisms. The reconstruction of detailed gene family histories, i.e., the precise dating of evolutionary events relative to phylogenetic tree of the underlying species has remained a challenging topic despite their importance as a basis for detailed investigations into adaptation and functional evolution of individual members of the gene family. The identification of orthologs, moreover, is a particularly important subproblem of the more general setting considered here. In the last few years, an extensive body of mathematical results has appeared that tightly links orthology, a formal notion of best matches among genes, and horizontal gene transfer. The purpose of this chapter is the broadly outline some of the key mathematical insights and to discuss their implication for practical applications. In particular, we focus on tree-free methods, i.e., methods to infer orthology or horizontal gene transfer as well as gene trees, species trees and reconciliations between them without using a priori knowledge of the underlying trees or statistical models for the inference of phylogenetic trees. Instead, the initial step aims to extract binary relations among genes. Copyright © 2023, The Authors. All rights reserved.

关键词： Genes

来源：评论

学校读者我要写书评

暂无评论

Integrated Analyses Identify a Master Microrna Regulatory Network for the Mesenchymal Subtype in Serous Ovarian Cancer (vol 23, pg 186, 2013)

引用

CANCER CELL 2013年第5期23卷 705-705页

作者： Yang, Da Sun, Yan Hu, Limei Zheng, Hong Ji, Ping Pecot, Chad V. Zhao, Yanrui Reynolds, Sheila Cheng, Hanyin Rupaimoole, Rajesha Cogdell, David Nykter, Matti Broaddus, Russell Rodriguez-Aguayo, Cristian Lopez-Berestein, Gabriel Liu, Jinsong Shmulevich, Ilya Sood, Anil K. Chen, Kexin Zhang, Wei Department of Pathology The University of Texas MD Anderson Cancer Center Houston TX 77030 USA Department of Gynecologic Oncology and Reproductive Medicine The University of Texas MD Anderson Cancer Center Houston TX 77030 USA Department of Cancer Biology The University of Texas MD Anderson Cancer Center Houston TX 77030 USA Department of Experimental Therapeutics The University of Texas MD Anderson Cancer Center Houston TX 77030 USA Center for RNAi and Non-Coding RNA The University of Texas MD Anderson Cancer Center Houston TX 77030 USA Division of Cancer Medicine The University of Texas MD Anderson Cancer Center Houston TX 77030 USA Department of Pathology Tianjin Medical University Cancer Institute and Hospital Tianjin 300060 China Department of Epidemiology and Biostatistics Tianjin Medical University Cancer Institute and Hospital Tianjin 300060 China Institute for Systems Biology Seattle WA 98103 USA Tampere University of Technology Tampere 33101 Finland

Integrated genomic analyses revealed a mirna-regulatory network that further defined a robust integrated mesenchymal subtype associated with poor overall survival in 459 cases of serous ovarian cancer (OvCa) from The Cancer Genome Atlas and 560 cases from independent cohorts. Eight key mirnas, including miR-506, miR-141, and miR-200a, were predicted to regulate 89% of the targets in this network. Follow-up functional experiments illustrate that miR-506 augmented E-cadherin expression, inhibited cell migration and invasion, and prevented TGFβ-induced epithelial-mesenchymal transition by targeting SNAI2, a transcriptional repressor of E-cadherin. In human OvCa, miR-506 expression was correlated with decreased SNAI2 and VIM, elevated E-cadherin, and beneficial prognosis. Nanoparticle delivery of miR-506 in orthotopic OvCa mouse models led to E-cadherin induction and reduced tumor growth.

关键词： Animals Apoptosis/drug effects Blotting, Western Blotting, Western Cadherins/genetics Cadherins/metabolism Cell Movement Cell Proliferation/drug effects Cystadenocarcinoma, Serous/genetics Cystadenocarcinoma, Serous/mortality Cystadenocarcinoma, Serous/pathology Epithelial-Mesenchymal Transition/genetics Female Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Immunoenzyme Techniques Immunoenzyme Techniques Mesoderm/metabolism Mesoderm/pathology Mice Mice, Nude Micrornas/genetics Micrornas/genetics Middle Aged Neoplasm Grading Ovarian Neoplasms/genetics Ovarian Neoplasms/mortality Ovarian Neoplasms/pathology Prognosis Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction rna, Messenger/genetics Survival Rate Transcription Factors/genetics Transcription Factors/metabolism Transforming Growth Factor beta/genetics Transforming Growth Factor beta/metabolism Tumor Cells, Cultured Tumor Markers, Biological/genetics Vimentin/genetics Vimentin/metabolism

来源：评论

学校读者我要写书评

暂无评论

Global, regional, and national burden of diabetes from 1990 to 2021, with projections of prevalence to 2050: a systematic analysis for the Global Burden of Disease Study 2021

引用

The Lancet 2023年第10397期402卷 203-234页

作者： Ong, Kanyin Liane Stafford, Lauryn K. McLaughlin, Susan A. Boyko, Edward J. Vollset, Stein Emil Smith, Amanda E. Dalton, Bronte E. Duprey, Joe Cruz, Jessica A. Hagins, Hailey Lindstedt, Paulina A. Aali, Amirali Abate, Yohannes Habtegiorgis Abate, Melsew Dagne Abbasian, Mohammadreza Abbasi-Kangevari, Zeinab Abbasi-Kangevari, Mohsen ElHafeez, Samar Abd Abd-Rabu, Rami Abdulah, Deldar Morad Abdullah, Abu Yousuf Md Abedi, Vida Abidi, Hassan Aboagye, Richard Gyan Abolhassani, Hassan Abu-Gharbieh, Eman Abu-Zaid, Ahmed Adane, Tigist Demssew Adane, Denberu Eshetie Addo, Isaac Yeboah Adegboye, Oyelola A. Adekanmbi, Victor Adepoju, Abiola Victor Adnani, Qorinah Estiningtyas Sakilah Afolabi, Rotimi Felix Agarwal, Gina Aghdam, Zahra Babaei Agudelo-Botero, Marcela Arriagada, Constanza Elizabeth Aguilera Agyemang-Duah, Williams Ahinkorah, Bright Opoku Ahmad, Danish Ahmad, Rizwan Ahmad, Sajjad Ahmad, Aqeel Ahmadi, Ali Ahmadi, Keivan Ahmed, Ali Ahmed, Luai A. Ahmed, Syed Anees Ajami, Marjan Akinyemi, Rufus Olusola Al Hamad, Hanadi Al Hasan, Syed Mahfuz AL-Ahdal, Tareq Mohammed Ali Alalwan, Tariq A. Al-Aly, Ziyad AlBataineh, Mohammad T. Alcalde-Rabanal, Jacqueline Elizabeth Alemi, Sharifullah Ali, Hassam Alinia, Tahereh Aljunid, Syed Mohamed Almustanyir, Sami Al-Raddadi, Rajaa M. Alvis-Guzman, Nelson Amare, Firehiwot Ameyaw, Edward Kwabena Amiri, Sohrab Amusa, Ganiyu Adeniyi Andrei, Catalina Liliana Anjana, Ranjit Mohan Ansar, Adnan Ansari, Golnoosh Ansari-Moghaddam, Alireza Anyasodor, Anayochukwu Edward Arabloo, Jalal Aravkin, Aleksandr Y. Areda, Demelash Arifin, Hidayat Arkew, Mesay Armocida, Benedetta Arnlov, Johan Artamonov, Anton A. Arulappan, Judie Aruleba, Raphael Taiwo Arumugam, Ashokan Aryan, Zahra Asemu, Mulu Tiruneh Asghari-Jafarabadi, Mohammad Askari, Elaheh Asmelash, Daniel Astell-Burt, Thomas Athar, Mohammad Athari, Seyyed Shamsadin Atout, Maha Moh'd Wahbi Avila-Burgos, Leticia Awaisu, Ahmed Azadnajafabad, Sina Darshan, B.B. Babamohamadi, Hassan Badar, Muhammad Badawi, Alaa Badiye, Ashish D. Baghcheghi, Nayereh Baghe Institute for Health Metrics and Evaluation School of Medicine General Medicine Service Department of Veterans Affairs Seattle WA United States Department of Health Metrics Sciences School of Medicine Faculty of Medicine France Department of Clinical Governance and Quality Improvement Aleta Wondo Hospital Aleta Wondo Ethiopia Department of Nursing Woldia University Woldia Ethiopia Department of Nursing Department of Orthopedic Surgery Orthopaedic Surgery Social Determinants of Health Research Center Non-communicable Diseases Research Center Epidemiology Department Evidence-Based Practice Center Community and Maternity Nursing Unit University of Duhok Duhok Iraq School of Planning Faculty of Environment Department of Public Health Sciences Penn State College of Medicine Pennsylvania State University Hershey PA United States Biocomplexity Institute Virginia Tech Blacksburg VA United States College of Medicine Department of Public Health Laboratory Technology Sciences Department Department of Family and Community Health University of Health and Allied Sciences Ho Ghana Research Center for Immunodeficiencies Department of Biosciences and Nutrition Karolinska University Hospital Huddinge Sweden Clinical Sciences Department Department of Surgery Department of Surgery Department of Obstetrics & Gynecology College of Graduate Health Sciences University of Tennessee Memphis TN United States Department of Obstetrics & Gynecology Department of Clinical and Psychosocial Epidemiology Department of Anesthesia and Critical Care Centre for Social Research in Health Quality and Systems Performance Unit Cancer Institute NSW Sydney NSW Australia Department of Public Health and Tropical Medicine HIV and Infectious Diseases Department Jhpiego Abuja Nigeria Adolescent Research and Care Department Adolescent Friendly Research Initiative and Care Ado Ekiti Nigeria Department of Epidemiology Department of Epidemiology and Medical Statistics Department of Family Medicine McMaster University

Background: Diabetes is one of the leading causes of death and disability worldwide, and affects people regardless of country, age group, or sex. Using the most recent evidentiary and analytical framework from the Global Burden of Diseases, Injuries, and Risk Factors Study (GBD), we produced location-specific, age-specific, and sex-specific estimates of diabetes prevalence and burden from 1990 to 2021, the proportion of type 1 and type 2 diabetes in 2021, the proportion of the type 2 diabetes burden attributable to selected risk factors, and projections of diabetes prevalence through 2050. Methods: Estimates of diabetes prevalence and burden were computed in 204 countries and territories, across 25 age groups, for males and females separately and combined;these estimates comprised lost years of healthy life, measured in disability-adjusted life-years (DALYs;defined as the sum of years of life lost [YLLs] and years lived with disability [YLDs]). We used the Cause of Death Ensemble model (CODEm) approach to estimate deaths due to diabetes, incorporating 25 666 location-years of data from vital registration and verbal autopsy reports in separate total (including both type 1 and type 2 diabetes) and type-specific models. Other forms of diabetes, including gestational and monogenic diabetes, were not explicitly modelled. Total and type 1 diabetes prevalence was estimated by use of a Bayesian meta-regression modelling tool, DisMod-MR 2.1, to analyse 1527 location-years of data from the scientific literature, survey microdata, and insurance claims;type 2 diabetes estimates were computed by subtracting type 1 diabetes from total estimates. Mortality and prevalence estimates, along with standard life expectancy and disability weights, were used to calculate YLLs, YLDs, and DALYs. When appropriate, we extrapolated estimates to a hypothetical population with a standardised age structure to allow comparison in populations with different age structures. We used the comparative r

关键词：

来源：评论

学校读者我要写书评

暂无评论

Chemical transformation motifs - Modelling pathways as integer hyperflows

arXiv

引用

arXiv 2017年

作者： Andersen, Jakob L. Flamm, Christoph Merkle, Daniel Stadler, Peter F. Earth-Life Science Institute Tokyo Institute of Technology Tokyo 152-8550 Japan Department of Mathematics and Computer Science University of Southern Denmark Odense M DK-5230 Denmark Research group Bioinformatics and Computational Biology Faculty of Computer Science University of Vienna Vienna 1090 Austria Institute for Theoretical Chemistry University of Vienna Wien 1090 Austria Halle-Jena-Leipzig and Competence Center for Scalable Data Services Solutions Dresden-Leipzig Leipzig Research Center for Civilization Diseases University of Leipzig Leipzig D-04107 Germany Max Planck Institute for Mathematics in the Sciences Leipzig D-04103 Germany Fraunhofer Institute for Cell Therapy and Immunology Leipzig D-04103 Germany Center for non-coding RNA in Technology and Health University of Copenhagen Frederiksberg C DK-1870 Denmark Santa Fe Institute 1399 Hyde Park Rd Santa Fe NM 87501 United States Research Network Chemistry Meets Microbiology University of Vienna Wien A-1090 Austria

We present an elaborate framework for formally modelling pathways in chemical reaction networks on a mechanistic level. Networks are modelled mathematically as directed multi-hypergraphs, with vertices corresponding to molecules and hyperedges to reactions. Pathways are modelled as integer hyperflows and we expand the network model by detailed routing constraints. In contrast to the more traditional approaches like Flux Balance Analysis or Elementary Mode analysis we insist on integer-valued flows. While this choice makes it necessary to solve possibly hard integer linear programs, it has the advantage that more detailed mechanistic questions can be formulated. It is thus possible to query networks for general transformation motifs, and to automatically enumerate optimal and near-optimal pathways. Similarities and differences between our work and traditional approaches in metabolic network analysis are discussed in detail. To demonstrate the applicability of the mathematical framework to real-life problems we first explore the design space of possible non-oxidative glycolysis pathways and show that recent manually designed pathways can be further optimised. We then use a model of sugar chemistry to investigate pathways in the autocatalytic formose process. A graph transformation-based approach is used to automatically generate the reaction networks of interest. Copyright © 2017, The Authors. All rights reserved.

关键词： Integer programming

来源：评论

学校读者我要写书评

暂无评论

Mapping geographical inequalities in childhood diarrhoeal morbidity and mortality in low-income and middle-income countries, 2000-17: analysis for the Global Burden of Disease Study 2017 (vol 395, pg 1779, 2020)

引用

LANCET 2020年第10239期395卷 1762-1762页

作者： Reiner, R. C., Jr. Hay, S., I Institute for Health Metrics and Evaluation University of Washington Seattle WA United States Department of Global Health School of Medicine University of Washington Seattle WA United States Department of Health Metrics Sciences School of Medicine University of Washington Seattle WA United States College of Health and Medical Sciences Haramaya University Harar Ethiopia Department of Epidemiology and Biostatistics Haramaya University Harar Ethiopia Department of Medical Laboratory Sciences Haramaya University Harar Ethiopia School of Nursing and Midwifery Haramaya University Harar Ethiopia School of Pharmacy Haramaya University Harar Ethiopia School of Public Health Haramaya University Harar Ethiopia Haramaya University Harar Ethiopia Advanced Diagnostic and Interventional Radiology Research Center Tehran University of Medical Sciences Tehran Iran Cancer Biology Research Center Tehran University of Medical Sciences Tehran Iran Cancer Research Institute Tehran University of Medical Sciences Tehran Iran Department of Economics and Management Sciences for Health Tehran University of Medical Sciences Tehran Iran Department of Environmental Health Engineering Tehran University of Medical Sciences Tehran Iran Department of Epidemiology and Biostatistics Tehran University of Medical Sciences Tehran Iran Department of Health Management and Economics Tehran University of Medical Sciences Tehran Iran Department of Microbiology Tehran University of Medical Sciences Tehran Iran Department of Pharmacology Tehran University of Medical Sciences Tehran Iran Digestive Diseases Research Institute Tehran University of Medical Sciences Tehran Iran Endocrinology and Metabolism Research Center Tehran University of Medical Sciences Tehran Iran Hematology-Oncology and Stem Cell Transplantation Research Center Tehran University of Medical Sciences Tehran Iran Iran National Institute of Health Research Tehran University of Medical Sciences Tehran Iran Metabolomics and

Summary Background Across low-income and middle-income countries (LMICs), one in ten deaths in children younger than 5 years is attributable to diarrhoea. The substantial between-country variation in both diarrhoea incidence and mortality is attributable to interventions that protect children, prevent infection, and treat disease. Identifying subnational regions with the highest burden and mapping associated risk factors can aid in reducing preventable childhood *** We used Bayesian model-based geostatistics and a geolocated dataset comprising 15 072 746 children younger than 5 years from 466 surveys in 94 LMICs, in combination with findings of the Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2017, to estimate posterior distributions of diarrhoea prevalence, incidence, and mortality from 2000 to 2017. From these data, we estimated the burden of diarrhoea at varying subnational levels (termed units) by spatially aggregating draws, and we investigated the drivers of subnational patterns by creating aggregated risk factor *** The greatest declines in diarrhoeal mortality were seen in south and southeast Asia and South America, where 54·0% (95% uncertainty interval [UI] 38·1-65·8), 17·4% (7·7-28·4), and 59·5% (34·2-86·9) of units, respectively, recorded decreases in deaths from diarrhoea greater than 10%. Although children in much of Africa remain at high risk of death due to diarrhoea, regions with the most deaths were outside Africa, with the highest mortality units located in Pakistan. Indonesia showed the greatest within-country geographical inequality; some regions had mortality rates nearly four times the average country rate. Reductions in mortality were correlated to improvements in water, sanitation, and hygiene (WASH) or reductions in child growth failure (CGF). Similarly, most high-risk areas had poor WASH, high CGF, or low oral rehydration therapy *** By co-analysing geospatial trends in d

关键词：

来源：评论

学校读者我要写书评

暂无评论

Author Correction: A bioabsorbable mechanoelectric fiber as electrical stimulation suture

引用

Nature communications 2025年第1期16卷 898页

作者： Zhouquan Sun Yuefan Jin Jiabei Luo Linpeng Li Yue Ding Yu Luo Yan Qi Yaogang Li Qinghong Zhang Kerui Li Haibo Shi Shankai Yin Hongzhi Wang Hui Wang Chengyi Hou State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and Engineering Donghua University Shanghai P. R. China. Shanghai Key Laboratory of Sleep Disordered Breathing Department of Otolaryngology-Head and Neck Surgery Otolaryngology Institute of Shanghai JiaoTong University Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai P. R. China. Shanghai Key Laboratory of Sleep Disordered Breathing Department of Otolaryngology-Head and Neck Surgery Otolaryngology Institute of Shanghai JiaoTong University Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai P. R. China. lilp@***. Department of General Surgery Tongji Hospital Tongji University Medical School Shanghai P. R. China. Shanghai Engineering Research Center of Pharmaceutical Intelligent Equipment Shanghai Frontiers Science Research Center for Druggability of Cardiovascular Non-coding RNA Institute for Frontier Medical Technology School of Chemistry and Chemical Engineering Shanghai University of Engineering Science Shanghai P. R. China. Yangzhi Rehabilitation Hospital Affiliated to Tongji University Tongji University School of Medicine Shanghai P. R. China. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and Engineering Donghua University Shanghai P. R. China. wanghz@***. Shanghai Key Laboratory of Sleep Disordered Breathing Department of Otolaryngology-Head and Neck Surgery Otolaryngology Institute of Shanghai JiaoTong University Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai P. R. China. wangh2005@alumni.***. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and Engineering Donghua University Shanghai P. R. China. hcy@dhu.edu.cn.

来源：评论

学校读者我要写书评

暂无评论

DNA origami technology for biomedical applications: Challenges and opportunities

MedComm - Biomaterials and Applications

引用

MedComm - Biomaterials and Applications 2023年第2期2卷

作者： Li, Ling Nie, Shihong Du, Ting Zhao, Jiaxuan Chen, Xiaoyuan West China School of Public Health West China Fourth Hospital and State Key Laboratory of Biotherapy Sichuan University Chengdu China Department of Radiation Oncology Cancer Center West China Hospital Sichuan University Chengdu China Non-coding RNA and Drug Discovery Key Laboratory of Sichuan Province Chengdu Medical College Chengdu China Departments of Diagnostic Radiology Surgery Chemical and Biomolecular Engineering and Biomedical Engineering Yong Loo Lin School of Medicine and Faculty of Engineering National University of Singapore Singapore Singapore Clinical Imaging Research Centre Centre for Translational Medicine Yong Loo Lin School of Medicine National University of Singapore Singapore Singapore Nanomedicine Translational Research Program NUS Center for Nanomedicine Yong Loo Lin School of Medicine National University of Singapore Singapore Singapore Institute of Molecular and Cell Biology Agency for Science Technology and Research (A*STAR) Singapore Singapore

DNA origami, a promising branch of structural DNA technology, refers to the technique of folding a single-stranded DNA scaffold into well-defined nanostructures. In recent years, DNA origami nanostructures have shown considerable promise in a variety of biomedical applications, owing to their biodegradability, unique programmability, and addressability. Despite their popularity, the biomedical application of DNA origami techniques, which exploits their unique programmability and addressability, is rare in previous studies. Most recently, mounting evidence has demonstrated the robustness of DNA origami nanostructures in the spatial organization of functional components at the nanoscale in the biomedical field. These examples provide typical paradigms to fully realize the potential of DNA origami techniques by taking advantage of their unique programmability and addressability. This minireview summarizes the recent advancements of DNA origami techniques in biosensing, biocatalysis, and drug delivery, and the representative examples using DNA origami nanostructures for the spatial organization of functional molecules with nanometric precision are highlighted. We further discuss the possible limitations and challenges for in vivo applications, including stability issues and potential immunogenicity, and finally, propose some strategies to overcome these obstacles to fully realize the potential of DNA origami techniques in biomedical applications. © 2023 The Authors. MedComm – Biomaterials and Applications published by John Wiley & Sons Australia, Ltd on behalf of Sichuan International Medical Exchange & Promotion Association (SCIMEA).

关键词： biocatalysis biosensing DNA nanotechnology DNA origami

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：