检索结果-内蒙古大学图书馆

The functional relationship between polymerization and catalytic activity of beef liver glutamate dehydrogenase. II. Experiment

引用

Journal of Molecular Biology 1976年第1期108卷 179-199页

作者： Cohen, Richard Jonathan Jedziniak, Judith A. Benedek, George B. Department of Physics Center for Materials Science Engineering Massachusetts Institute of Technology Cambridge MA 02139 United States Harvard-M.I.T. Program in Health Sciences and Technology Massachusetts Institute of Technology Cambridge MA 02139 United States

We present quasi-elastic light-scattering spectroscopic measurements of the polymer distribution and parallel determinations of the catalytic activity of bovine glutamate dehydrogenase in solution, in order to examine the relationship between the polymer distribution and the enzymatic activity over a wide range of enzyme and effector concentrations. In the absence of ligands, we find that the mode of aggregation is one of reversible linear end-to-end polymerization into rigid rods, characterized by a single polymerization constant. In the presence of ligands, including effectors, the determinations of polymer distribution and enzymatic activity may be analyzed in terms of the two-species (x monomer and y monomer) theoretical models presented in the previous paper. Of the three models presented, the best agreement with the experimental data achieved by Model II in which the x-y binding constant is the geometric mean of the x-x and y-y binding constants. In terms of this model all of the experimental data may be quantitatively accounted for, utilizing a minimum number of independently measured parameters. The experimental evidence supports the theoretical prediction that the polymerization process plays an important role in establishing the regulatory properties of this enzyme. © 1976 Academic Press Inc.

关键词：

来源：评论

学校读者我要写书评

暂无评论

Bone Cement 1

引用

丛书名： SpringerBriefs in Applied sciences and Technology

1000年

作者： Hamid Reza Rezaie Mohammad Hossein Esnaashary Masoud Karfarma Andreas Öchsner

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：