Metabolic fluxes have been regarded as an important quantity for metabolic engineering as they reveal cause-effect relationships between genetic modifications and resulting changes in metabolic activity and are used a...
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Metabolic fluxes have been regarded as an important quantity for metabolic engineering as they reveal cause-effect relationships between genetic modifications and resulting changes in metabolic activity and are used as a prerequisite for the design of optimal whole cell biocatalysts. The intracellular fluxes must be estimated due to the inability to measure them directly. A particular useful technique involves the use of /sup 13/C-enriched substrates and the measurement of label distribution generated for each intermediate to uncover all unmeasured fluxes by solving the label balance equations, e.g. isotopomer balances, at steady state. However, the formation of these equations typically requires tedious algebraic manipulation and in many cases the resulting equations must be solved numerically, due to the nonlinearity and high dimensionality. Here we present three different evolutionary algorithm (EA) based approaches in combination with the least squares algorithm to show the applicability of EAs in metabolic flux quantification. The performance of the algorithms are illustrated and discussed through the simulation of the cyclic pentose phosphate network in a noisy environment and the identifiability problem is also considered.
The estimation of parameters and obtaining an accurate and comprehensive mathematical model of the polymerization process is of strategic importance to the controlengineering purposes in the polymerization industry. ...
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The estimation of parameters and obtaining an accurate and comprehensive mathematical model of the polymerization process is of strategic importance to the controlengineering purposes in the polymerization industry. It is characteristic for these processes a grate non-linearity and many difficulties applying traditional estimation techniques. This paper describes an approach based upon neural-fuzzy representation of the model. A concrete model is constructed with the Sugeno fuzzy inference technique and a fuzzy-neural network is used to model the dynamic behavior of the polymer process. Such neural-fuzzy models of polymer quality could be used successfully for optimization and control of polymerization processes. Short example for such implementation is included with additional results for modeling of Mn and Mw.
Model predictive control (MPC) is presented as a tactical decision module for supply chain management in semiconductor manufacturing. A representative problem which includes distinguishing features of semiconductor ma...
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Model predictive control (MPC) is presented as a tactical decision module for supply chain management in semiconductor manufacturing. A representative problem which includes distinguishing features of semiconductor manufacturing supply chains, such as material reconfiguration and stochastic product splits, is examined. Fluid analogies are used to model the supply chain dynamics, with stochasticity and nonlinearity occurring on the throughput time, yield and customer demand. Given inventory targets and capacity limits, MPC using linear time invariant models can make the system outputs track the targets and improve customer service levels. The flexibility provided by the choice of tuning parameters in MPC to achieve better performance and robustness in semiconductor manufacturing supply chain management is demonstrated.
Realizing steady and reliable navigation is a prerequisite for a mobile robot, but this facility is often weakened by an unavoidable slip or some irreparable drift errors of sensors in long-distance navigation. Althou...
Realizing steady and reliable navigation is a prerequisite for a mobile robot, but this facility is often weakened by an unavoidable slip or some irreparable drift errors of sensors in long-distance navigation. Although perceptual landmarks were solutions to such problems, it is impossible not to miss landmarks occasionally at some specific spots when the robot moves at different speeds, especially at higher speeds. If the landmarks are put at random intervals, or if the illumination conditions are not good, the landmarks will be easier to miss. In order to detect and extract artificial landmarks robustly under multiple illumination conditions, some low-level but robust image processing techniques were implemented. The moving speed and self-location were controlled by the visual servo control method. In cases where a robot suddenly misses some specific landmarks when it is moving, it will find them again in a short time based on its intelligence and the inertia of the previous search motion. These methods were verified by the reliable vision-based indoor navigation of an A-life mobile robot.
This paper investigates the robustness of dual-rate MPC systems with a proposed inferential control strategy. It shows that for some scenarios where a high-frequency model plant mismatch is presented, such dual-rate i...
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This paper investigates the robustness of dual-rate MPC systems with a proposed inferential control strategy. It shows that for some scenarios where a high-frequency model plant mismatch is presented, such dual-rate inferential MPC systems may be more robust than fast single rate MPC systems.
The chemicalengineering program at Arizona State offers an integrated series of core courses that teach students how conservation and accounting principles can be applied to describe engineering phenomena across disc...
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Two laboratory-scale experiments have been developed that can be executed safely over the Internet. They provide practical experience for students of process control. Remote access is within a client-server paradigm. ...
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Two laboratory-scale experiments have been developed that can be executed safely over the Internet. They provide practical experience for students of process control. Remote access is within a client-server paradigm. Servers connected to the processes through the usual data acquisition hardware address issues regarding safety, security, data validation, and session management. A unique feature of the lab is the ability for the student to design, test, and verify control strategies in real time over the Internet. Necessary interlocks and cutoffs ensure safe remote operation of the units. Studies have shown that network speeds are not a significant concern for these systems due to slow process dynamics. The availability of Internet-mediated laboratory modules on an anytime/anywhere basis will enable a larger fraction of the class to get valuable laboratory experience. These experiments have also been used in an Internet-wired classroom for interactive demonstrations during lecture.
The chemicalengineering program at Arizona State offers an integrated series of core courses that teach students how conservation and accounting principles can be applied to describe engineering phenomena across disc...
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The chemicalengineering program at Arizona State offers an integrated series of core courses that teach students how conservation and accounting principles can be applied to describe engineering phenomena across disciplines. A brine-water mixing tank experiment was introduced in the third course in the series (ECE 394C: Understanding engineeringsystems Via Conservation) as a capstone modeling project for the recitation portion of the course. The experiment provides students with "hands-on" experience on a real-life system incorporating process, electrical, and mechanical components, as well as real-time data acquisition and control. A major feature of the brine-water tank project is that students apply a comprehensive system identification procedure relying on semiphysical (a.k.a. "grey box") models to complement their understanding of first-principles modeling. This paper describes the brine-water tank experiment, presents the formulation of the semiphysical parameter estimation problem, and describes the comprehensive procedure that students undertake to go from process data to validated plant models.
The term "plant-friendly" system identification has been used within the chemical process control research community in reference to the broad-based goal of accomplishing informative identification testing w...
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The term "plant-friendly" system identification has been used within the chemical process control research community in reference to the broad-based goal of accomplishing informative identification testing while meeting the demands of industrial practice. While many different identification topics (such as control-relevant identification, closed-loop identification and optimal input design) can be said to contribute to plant-friendliness in identification, the problem has some unique character of its own. This paper describes some of the issues that motivate plant-friendly identification and presents an overview of some approaches that have been proposed in this topic. The problem of identification test monitoring is presented as a novel means for accomplishing plant-friendly identification.
Measurement error due to sensor degradation (fouling, miscalibration, etc.) is more difficult to identify compared to catastrophic sensor failure. Passive methods previously proposed for sensor-level monitoring are ba...
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Measurement error due to sensor degradation (fouling, miscalibration, etc.) is more difficult to identify compared to catastrophic sensor failure. Passive methods previously proposed for sensor-level monitoring are based on power spectrum or multiscale analysis of sensor data. These methods have limitations caused by not accounting for various noise sources and assumptions about sensor noise characteristics, thus resulting in false and missed alarms. In this paper, an online sensor fault detection scheme based on the identification of sensor response characteristics is proposed and evaluated. We develop both robust passive and active in situ techniques to identify sensor response characteristics that relate directly to its health. Using the identified sensor model, various kinds of sensor faults are quantified and mapped into the model parameters. A dynamic model-based estimator is proposed for data reconciliation. These ideas were experimentally validated using thermocouples, flowmeters, and resistance thermometric devices on laboratory-scale processes. The proposed approach was seen to accurately quantify the sensor model parameters and aid in measurement reconstruction.
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