We present the use of the polysaccharide chitosan for immobilizing biomolecules on microfabricated device surfaces. The main advantages of chitosan are its abundance of primary amine groups and its ability to be elect...
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ISBN:
(纸本)1424402778
We present the use of the polysaccharide chitosan for immobilizing biomolecules on microfabricated device surfaces. The main advantages of chitosan are its abundance of primary amine groups and its ability to be electrodeposited. Biomolecules are easily attached to chitosan 's amines by standard glutaraldehyde chemistry. The electrodeposition of chitosan allows accurate spatial and temporal control of biomolecule placement. We have demonstrated this biofunctionalization method for two different bioMEMS devices: a biophotonic sensor and a micromechanical biosensor. We have successfully assembled probe DNA on each of them and detected hybridization with target DNA as a demonstration of sensor operation. Here, we briefly present the fabrication procedure and testing results for both of these bioMEMS sensing devices
In the past two decades, calcium phosphates, and especially hydroxyapatite (HA), have been used as bone substitutes, and the mechanical strength is one of the most important properties in this application. The flexura...
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ISBN:
(纸本)9781617820632
In the past two decades, calcium phosphates, and especially hydroxyapatite (HA), have been used as bone substitutes, and the mechanical strength is one of the most important properties in this application. The flexural strength of an HA object depends on the nature of the HA powder, specimen preparation, flex test geometry and experimental conditions. HA was prepared by two methods, namely: wet and hydrothermal. The fundamental difference between these methods lies in the method of mixing the reactants and the reaction temperature. The obtained powders were pressed into pellets at 150 MPa and tested for flexural strength using a ring-on-disc method. The two-parameter Weibull and normal distributions were used to characterize the observed flexural strengths. Results were correlated with scanning microscope observations of fracture surfaces of the polished broken discs. The two-parameter Weibull distribution was found to be superior for the samples made with HA from the hydrothermal method. For the samples from the wet method, the normal distribution was equally suitable. The reason for this difference was sought by examining the discs for surface flaws.
作者：
Lu SunLei ZhuLixia RongBenjamin S. HsiaoPolymer Program
Institute of Materials Science and Department of Chemical Materials and Biomolecular Engineering University of Connecticut Storrs CT 06269‐3136 USA Fax: (+1) 860‐486‐4745 http://www.ims.uconn.edu/∼lzhu Department of Chemistry
State University of New York at Stony Brook Stony Brook NY 11794‐3400 USA
Several research efforts are being carried out in molecular electronics;both theory and experiment claim extraordinary findings for molecular devices. However, before practical molecular circuits can be implemented, w...
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Composite membranes of sulfonated crosslinked polystyrene particles have been made by aligning the particles under an electric field in a crosslinked poly(dimethyl siloxane) matrix following a procedure described by O...
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Composite membranes of sulfonated crosslinked polystyrene particles have been made by aligning the particles under an electric field in a crosslinked poly(dimethyl siloxane) matrix following a procedure described by Oren et al. (2004). The purpose of this work, however, was to investigate the effects of electric field amplitude and frequency on alignment behavior. As expected, the particles aligned only by application of a certain minimum electric field for a given frequency. Impedance measurements indicated that the unaligned membranes showed capacitive behavior up to 8 wt % particles, which transformed to conductive behavior on alignment.
This paper presents an integrated microfluidic device for the generation of poly (lactideco- glycolide) (PLGA) nanospheres with protein coating. The chip utilizes the microfluidic droplet reactor technique [1] and PLG...
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Powder-metallurgy processed high-speed steels containing up to 5 wt.% of Nb were fabricated by nitrogen gas atomization of elemental powders followed by hot isostatic pressing and hot working. Microstructural changes,...
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