检索结果-内蒙古大学图书馆

Intranasal delivery of engineered extracellular vesicles promotes neurofunctional recovery in traumatic brain injury

引用

JOURNAL OF NANOBIOTECHNOLOGY 2025年第1期23卷 229页

作者： Li, Pengtao Sun, Sishuai Zhu, Xingyu Liu, Xiaoyu Yin, Rui Chen, Yihao Chang, Jianbo Ye, Liguo Gao, Jingxi Zhao, Xiaoyan Xu, Houshi Wang, Yue Zuo, Wei Sun, Zhao Wang, Shihua Zhang, Xiao Wei, Junji Zhao, Robert Chunhua Han, Qin Chinese Acad Med Sci Peking Union Med Coll Hosp Peking Union Med Coll Dept Neurosurg Beijing Peoples R China Chinese Acad Med Sci Inst Basic Med Sci Peking Union Med Coll Sch Basic Med Beijing Peoples R China Chinese Acad Med Sci Peking Union Med Coll Hosp Peking Union Med Coll Dept Oncol Beijing Peoples R China

Traumatic brain injury (TBI) is a leading cause of disability in adults, significantly affecting patients' quality of life. Extracellular vesicles (EVs) derived from human adipose-derived mesenchymal stem cells (hADSCs) have demonstrated therapeutic potential in TBI treatment. However, their limited targeting ability, short half-life, and low bioavailability present significant challenges for clinical application. In this study, we engineered extracellular vesicles (EEVs) by transfecting hADSCs with lentivirus and incorporating ultra-small paramagnetic nanoparticles (USPNs), resulting in EVs with enhanced miRNA expression and targeted delivery capabilities. These EEVs were administered intranasally to specifically target injury sites, effectively modulating the NF-kappa B signaling pathway to suppress neuroinflammation. In both in vitro and in vivo assessments, EEVs exhibited superior efficacy in promoting neurofunctional recovery and neurogenesis after brain injury compared to unmodified EVs. Furthermore, validation using human brain organoid models confirmed EEVs' remarkable ability to suppress neuroinflammation, offering a promising strategy for TBI treatment.

关键词： Traumatic brain injury Engineered extracellular vesicles NF-kappa B Neuroinflammation Neurofunctional recovery

来源：评论

学校读者我要写书评

暂无评论

Innate Immunity in Helicobacter pylori Infection and Gastric Oncogenesis

引用

HELICOBACTER 2025年第2期30卷 e70015页

作者： Zhang, Yuheng Yan, Zhiyu Jiao, Yuhao Feng, Yunlu Zhang, Shengyu Yang, Aiming Chinese Acad Med Sci & Peking Union Med Coll Peking Union Med Coll Hosp Dept Gastroenterol Beijing Peoples R China Chinese Acad Med Sci & Peking Union Med Coll Peking Union Med Coll Eight Year Med Doctor Program Beijing Peoples R China Chinese Acad Med Sci & Peking Union Med Coll Peking Union Med Coll Hosp Dept Med Beijing Peoples R China

Helicobacter pylori is an extremely common cause of gastritis that can lead to gastric adenocarcinoma over time. Approximately half of the world's population is infected with H. pylori, making gastric cancer the fourth leading cause of cancer-related deaths worldwide. Innate immunity significantly contributes to systemic and local immune responses, maintains homeostasis, and serves as the vital link to adaptive immunity, and in doing so, mediates H. pylori infection outcomes and consequent cancer risk and development. The gastric innate immune system, composed of gastric epithelial and myeloid cells, is uniquely challenged by its need to interact simultaneously and precisely with commensal microbiota, exogenous pathogens, ingested substances, and endogenous exfoliated cells. Additionally, innate immunity can be detrimental by promoting chronic infection and fibrosis, creating an environment conducive to tumor development. This review summarizes and discusses the complex role of innate immunity in H. pylori infection and subsequent gastric oncogenesis, and in doing so, provides insights into how these pathways can be exploited to improve prevention and treatment.

关键词： gastric cancer gastric epithelial cells Helicobacter pylori infection innate immunity mucosal immunology

来源：评论

学校读者我要写书评

暂无评论

Risk factors of infective endocarditis-associated acute kidney injury: benefits of low-dose amikacin and surgery

引用

ANNALS OF medICINE 2025年第1期57卷 2482023页

作者： Ai, Sanxi Liu, Xinpei Zhao, Nan Miao, Qi Qin, Yan Li, Xuemei Chinese Acad Med Sci & Peking Union Med Coll Peking Union Med Coll Hosp Dept Nephrol Beijing Peoples R China Chinese Acad Med Sci & Peking Union Med Coll Peking Union Med Coll Hosp Dept Cardiac Surg Beijing Peoples R China Chinese Acad Med Sci & Peking Union Med Coll Peking Union Med Coll Hosp Dept Med Res Beijing Peoples R China

Objective Acute kidney injury (AKI) is a frequent presentation in patients with infective endocarditis (IE), and is associated with poor outcomes. The primary aim of the study was to determine the risk factors for AKI in patients with IE. Methods A retrospective study was conducted in a tertiary hospital in China from August 2012 to April 2022. The primary outcome was AKI. Logistic regression was used to identify risk factors for AKI. Results A total of 594 patients with IE were included, of which 256 (43.1%) experienced AKI. The following variables were found to be independent risk factors for AKI: hypertension (OR 1.96, p = 0.011), Staphylococcus aureus infection (OR 2.69, p = 0.008), heart failure (OR 5.99, p < 0.001), shock (OR 5.93, p = 0.015) and hematuria (OR 2.38, p < 0.001). The use of low-dose amikacin (400 mg daily) was associated with a lower incidence of AKI (OR 0.38, p < 0.001). Among patients with IE complicated by heart failure, surgery was associated with a lower incidence of AKI (OR 0.21, p = 0.010). Conclusion AKI is common in IE. Hypertension, Staphylococcus aureus infection, heart failure, shock and hematuria are risk factors of IE-associated AKI, whereas low-dose amikacin is a protective factor. Surgery is a protective factor of AKI in the setting of IE complicated by heart failure.

关键词： Acute kidney injury risk factor amikacin infective endocarditis surgery

来源：评论

学校读者我要写书评

暂无评论

Aldehyde Dehydrogenase 2 Lactylation Aggravates Mitochondrial Dysfunction by Disrupting PHB2 mediated Mitophagy in Acute Kidney Injury

引用

ADVANCED sciENCE 2025年第8期12卷 e2411943页

作者： Li, Jiaying Shi, Xiaoxiao Xu, Jiatong Wang, Kaiyue Hou, Fangxing Luan, Xiaodong Chen, Limeng Chinese Acad Med Sci & Peking Union Med Coll Peking Union Med Coll Hosp Dept Nephrol State Key Lab Complex Severe & Rare Dis Beijing 100730 Peoples R China Chinese Acad Med Sci & Peking Union Med Coll Dept Internal Med Peking Union Med Coll Hosp Beijing 100730 Peoples R China Chinese Acad Med Sci & Peking Union Med Coll Peking Union Med Coll Hosp Inst Clin Med Ctr Drug Res & Evaluat Beijing 100730 Peoples R China

Mitochondrial dysfunction is a crucial event in acute kidney injury (AKI), leading to a metabolic shift toward glycolysis and increased lactate production. Lactylation, a posttranslational modification derived from lactate, plays a significant role in various cellular processes, yet its implications in AKI remain underexplored. Here, a marked increase in lactate levels and pan-Kla levels are observed in kidney tissue from AKI patients and mice, with pronounced lactylation activity in injured proximal tubular cells identified by single-cell RNA sequencing. The lactylation of aldehyde dehydrogenase 2 (ALDH2) is identified at lysine 52 (K52la), revealing that ALDH2 lactylation exacerbates tubular injury and mitochondrial dysfunction. Conversely, the ALDH2 K52R mutation alleviates these injuries in HK-2 cells and adeno-associated virus-infected kidney tissues in mice. Furthermore, ALDH2 lactylation can be modulated by upregulating SIRT3 in vivo and in vitro, which reduces ALDH2 lactylation, mitigating tubular injury and mitochondrial dysfunction. Mechanistically, immunoprecipitation-mass spectrometry analysis demonstrates an interaction between ALDH2 and prohibitin 2 (PHB2), a crucial mitophagy receptor. ALDH2 lactylation promotes the ubiquitination-proteasomal degradation of PHB2 to inhibit mitophagy and worsen mitochondrial dysfunction. These findings highlight the critical role of endogenous lactate in AKI and propose ALDH2 lactylation as a potential therapeutic target.

关键词： acute kidney injury ALHD2 lactylation mitochondrial function mitophagy

来源：评论

学校读者我要写书评

暂无评论

Comparative Analysis of Muscle Elasticity and Pathological Characteristics Between Affected and Unaffected Sides in Postherpetic Neuralgia Patients: Protocol for a Pilot Cohort Trail

引用

JOURNAL OF PAIN RESEARCH 2025年 18卷 1597-1605页

作者： Ji, Heyu Ma, Jiangyu Cui, Xulei Huang, Yuguang Chinese Acad Med Sci & Peking Union Med Coll Peking Union Med Coll Hosp Dept Anesthesiol Beijing Peoples R China Chinese Acad Med Sci & Peking Union Med Coll Peking Union Med Coll Hosp Dept Nucl Med Beijing Peoples R China

Background: Postherpetic neuralgia (PHN) is the most common condition that can develop as a complication after herpes zoster (HZ) infection, characterized by pain that persists for more than 3 months after the initial rash has resolved. In most patients with HZ, the rash appears unilaterally. While the treatment of PHN is primarily focused on neural mechanisms due to HZ's neurotropism nature, recent evidence suggests that muscle tissues within the affected regions may also experience pathological changes that contribute to the pain. These changes could reveal novel therapeutic targets and enhance patient prognosis. This study aims to investigate these muscular changes and explore myogenic pain mechanisms in PHN patients. It employs ultrasound elastography to compare muscle elasticity between the affected and unaffected sides and conduct muscle biopsies for pathophysiological analysis to uncover the underlying mechanisms. Materials and Methods: This comparative cross-sectional study aims to enroll 30 PHN patients. The primary outcome is the comparison of muscle elasticity on the affected sides with unaffected sides. The secondary outcome is from muscle biopsies, which are obtained and analyzed by histopathological techniques. Pain levels before and after therapy are assessed using the Numerical Rating Scale (NRS), with follow-up to evaluate outcomes and satisfaction. Statistical analysis will employ paired t-tests or Wilcoxon signed-rank tests to compare muscle elasticity, and correlation analysis to explore the relationship between elasticity and pathological findings. Hypothesis: The study hypothesis is that muscle elasticity on the affected side is significantly higher than on the unaffected side, with the coexistence of myofascial pain. This myofascial pain may overlap with PHN pain and may be a source of discomfort in refractory PHN cases. Furthermore, muscle biopsies are conducted to clarify pathological changes. This study may pave the way for novel treatment

关键词： postherpetic neuralgia shear wave elastography myofascial pain muscle biopsy

来源：评论

学校读者我要写书评

暂无评论

Distinct clinical characteristics and prognosis of pediatric-onset growth hormone-secreting pituitary adenoma (GHPA) patients compared to adult-onset patients

引用

ENDOCRINE 2025年第1期87卷 295-303页

作者： Zheng, Xue-Qing Yang, Sheng-Min Xiao, Tong-Xin Yang, Ying-Ying Sun, Yu-Xin Li, Ran Liu, Jie Huang, Huai-Gu Yao, Yong Deng, Kan Lian, Xin You, Hui Duan, Lian Zhu, Hui-Juan Chinese Acad Med Sci & Peking Union Med Coll State Key Lab Complex Severe & Rare Dis Key Lab Endocrinol Natl Hlth Commiss Dept EndocrinolPeking Union Med Coll Hosp Beijing Peoples R China Chinese Acad Med Sci Peking Union Med Coll Beijing Peoples R China Chinese Acad Med Sci & Peking Union Med Coll Peking Union Med Coll Hosp Dept Neurosurg Beijing Peoples R China Chinese Acad Med Sci & Peking Union Med Coll Peking Union Med Coll Hosp Dept Radiotherapy Beijing Peoples R China Chinese Acad Med Sci & Peking Union Med Coll Peking Union Med Coll Hosp Dept Radiol Beijing Peoples R China

PurposeTo explore the clinical characteristics, treatment, and prognosis of growth hormone-secreting pituitary adenoma (GHPA) patients with pediatric-onset, so as to facilitate clinical *** retrospective cohort study was carried out between 102 pediatric-onset GHPA patients admitted to our hospital from January 2013 to June 2022 and 204 adult-onset GHPA patients who were randomly *** with pediatric-onset was predominantly male, associated with higher proportion of genetic syndromes, longer course, and delayed diagnosis. Clinical symptoms of visual field defects and menstrual abnormality were more common. The pediatric-onset group exhibited higher growth hormone (GH) nadir during oral glucose tolerance test (OGTT), higher rates of hyperprolactinemia, larger maximum diameter of adenoma, and higher rates of optic chiasm compression, suprasellar invasion, and pituitary apoplexy. Hypertension, diabetes, and obstructive sleep apnea-hypopnea syndrome (OSAHS) were more common in the adult-onset group. Echocardiography results were similar between the two groups. The pediatric-onset group owned significantly higher treatment scores and proportions of multimodal therapy modality, more surgical complications, and a higher proportion of ki67 >= 3%. There was no significant difference in the final cure rate, but male patients with adult-onset had a worse prognosis. The recurrence rate was also similar between two groups. Hypopituitarism was more prevalent in the pediatric-onset group, while the adult-onset group had a higher incidence of other ***-onset GHPA patients exhibit distinct clinical characteristics compared to adult-onset patients. Multimodal therapy modalities could help to achieve a cure rate comparable to that of adult-onset patients.

关键词： Pediatric-onset growth hormone secreting pituitary adenoma Clinical characteristics Metabolism Treatment prognosis

来源：评论

学校读者我要写书评

暂无评论

Unicentric Castleman disease complicated with bronchiolitis obliterans: A single-centre retrospective study from China

引用

BRITISH JOURNAL OF HAEMATOLOGY 2025年第4期206卷 1129-1135页

作者： Liu, Yan-ting Zhen, Jun-feng Gao, Yu-han Li, Si-yuan Dang, Yue Xu, Hao-Yi Zhang, Lu Li, Jian Chinese Acad Med Sci & Peking Union Med Coll Peking Union Med Coll Hosp Dept Hematol Beijing Peoples R China Chinese Acad Med Sci & Peking Union Med Coll Peking Union Med Coll Hosp Dept Int Med Serv Beijing Peoples R China

Bronchiolitis obliterans (BO) is a rare and life-threatening complication of unicentric Castleman disease (UCD), which is considered the leading cause of death in UCD. However, few studies focused on the characteristics of UCD patients complicated with BO. This single-centre, retrospective study of 281 patients diagnosed with UCD identified 18 patients (6.4%) with BO. UCD-BO patients had a significantly lower age at UCD diagnosis than the UCD without BO group. 94.4% of UCD-BO patients had deep lymph nodes involvement and 83.3% of them had paraneoplastic pemphigus, which were both significantly higher than UCD without BO. median forced expiratory volume in 1 s of predicted was 20.9% (10%-41.9%) and 83.3% patients had severe BO. All patients underwent complete resection for UCD. 61.1% patients developed BO after surgery. UCD-BO patients had a poorer overall survival than UCD without BO. Among severe BO, 11 patients did not receive lung transplantation and two of them died, with 62.5% (95% CI, 20.8%-100%) of estimated 5-year overall survival. Four patients underwent lung transplantation and they all survived. UCD-BO patients had distinct clinical identity. Complete resection could neither alleviate BO nor prevent BO progression. Lung transplantation is a vital treatment for improving prognosis for patients with severe BO.

关键词： bronchiolitis obliterans lung transplantation paraneoplastic pemphigus unicentric Castleman disease

来源：评论

学校读者我要写书评

暂无评论

Exploring the Association Between Food Insecurity, Bowel Health, and All-Cause Mortality in the United States: Insights from NHANES 2005-2010

引用

JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY 2025年第3期40卷 653-665页

作者： Li, Yunlong Chen, Yang Yu, Ziqing Bai, Xiaoyin Ruan, Gechong Sun, Yinghao Han, Wei Yang, Hong Li, Xiaoqing Chinese Acad Med Sci & Peking Union Med Coll Peking Union Med Coll Hosp Dept Gastroenterol Beijing Peoples R China Chinese Acad Med Sci & Peking Union Med Coll Peking Union Med Coll Hosp Dept Epidemiol & Biostat Beijing Peoples R China

Background and AimFunctional gastrointestinal disorders such as chronic constipation (CC) and chronic diarrhea (CD) impose a significant burden on global healthcare resources. Food insecurity, a crucial social determinant of health, remains unclearly associated with CC and CD. We aim to explore the association between food insecurity and CC and CD, and its impact on all-cause mortality among participants with abnormal bowel *** used data from National Health and Nutrition Survey 2005-2010. We assessed food insecurity using the Household Food Security Survey Module, and bowel health, including CD and CC, was evaluated using fecal characteristics and bowel movement frequency from the Bowel Health Questionnaire. Finally, 13 199 participants were *** total of 15.26% (2014) participants experienced food insecurity. Multivariate analysis revealed that food insecurity was associated with a higher proportion of CD (odds ratio [OR]: 1.24, 95% confidence interval [CI]: 1.02-1.52, p = 0.03), especially in women. CC did not exhibit a significant association with food insecurity (OR: 1.10, CI: 0.86-1.40, p = 0.41). Furthermore, food insecurity was significantly linked to higher all-cause mortality in patients with CD (OR: 1.59, CI: 1.01-2.50, p = 0.046) and CC (OR: 2.01, CI: 1.20-3.46, p = 0.01).ConclusionsAssessing food insecurity's impact on bowel health is crucial for policy formulation and risk identification. Research should explore links between food insecurity and CD or CC severity, treatment, and psychosocial aspects to improve patient quality of life.

关键词： bowel health chronic constipation chronic diarrhea food insecurity NHANES

来源：评论

学校读者我要写书评

暂无评论

Advances in laboratory diagnosis of Sjogren's disease in children

引用

CLINICA CHIMICA ACTA 2025年 567卷 120095页

作者： Li, Yuemeng He, Wenxiu Zhou, Yu Chen, Haotian You, Pengyue Mu, Danni Ma, Yichen Gao, Yumeng Xu, Kaiduo Dong, Haitao Cheng, Xinqi Peking Union Med Coll & Chinese Acad Med Sci Peking Union Med Coll Hosp Dept Lab Med Beijing 100730 Peoples R China Peking Union Med Coll & Chinese Acad Med Sci Peking Union Med Coll Hosp Dept Stomatol Beijing 100730 Peoples R China Peking Union Med Coll & Chinese Acad Med Sci Peking Union Med Coll Hosp Dept Pediat Beijing 100730 Peoples R China Beijing Hosp Inst Geriatr Med Chinese Acad Med Sci Natl Ctr GerontolNatl Ctr Clin Labs Beijing 100730 Peoples R China Chinese Acad Med Sci & Peking Union Med Coll Beijing 100730 Peoples R China

Sjogren's disease (SjD) in children is a rare chronic autoimmune disease not fully recognized due to clinical manifestations different from adults. As such, new objective indicators are needed to supplement existing markers and assist in diagnosis. This review summarizes pathogenesis of SjD in children, current diagnostic criteria and research progress in laboratory diagnosis including serologic testing, saliva and tear analysis, histopathological examination as well as emerging markers of interest.

关键词： Sjogren's disease Biomarkers Laboratory diagnosis Exosomes

来源：评论

学校读者我要写书评

暂无评论

Immune Signatures of Solid Tumor Patients Treated With Immune Checkpoint Inhibitors: An Observational Study

引用

THORACIC CANCER 2025年第1期16卷 e15493页

作者： Chen, Ling Tan, Hourui Geng, Ruixuan Li, Yifan Wang, Yingyi Li, Taisheng Chinese Acad Med Sci & Peking Union Med Coll Peking Union Med Coll Hosp Dept Infect Dis Beijing Peoples R China Chinese Acad Med Sci & Peking Union Med Coll Peking Union Med Coll Hosp Dept Med Oncol Beijing Peoples R China Chinese Acad Med Sci & Peking Union Med Coll Peking Union Med Coll Hosp Dept Int Med Serv Beijing Peoples R China Chinese Acad Med Sci & Peking Union Med Coll Natl Canc Ctr Natl Clin Res Ctr Canc Dept Gynecol OncolCanc Hosp Beijing Peoples R China Chinese Acad Med Sci & Peking Union Med Coll Peking Union Med Coll Hosp State Key Lab Complex Severe & Rare Dis Beijing Peoples R China

PurposeOur study aimed to comprehensively describe the features of peripheral blood multiple immune cell phenotypes in solid tumor patients during pretreatment and after immunotherapy, providing a more convenient approach for studying the prognosis of immunotherapy in different solid tumor *** prospectively recruited patients with advanced solid tumors from peking union medical college Hospital (PUMCH) between February 2023 and April 2024. Using multicolor flow cytometry, our study comprehensively observed and described the signatures of peripheral blood lymphocyte subsets including activation, proliferation, function, na & iuml;ve memory, and T cell exhaustion immune cell subsets in this population of pretreatment and after *** study enrolled 59 advanced solid tumor patients with immunotherapy and 59 healthy controls were matched by age and gender. The results demonstrated a marked upregulation in the expression of lymphocyte activation markers CD38 and HLA-DR, as well as exhaustion and proliferation markers PD-1 and Ki67, in solid tumor patients compared to healthy controls. After immune checkpoint blockade (ICB) treatment, mainly the expression of Ki67CD4+T and HLA-DRCD38CD4+T, was significantly upregulated compared to pretreatment levels (p = 0.017, p = 0.019, respectively). We further found that gynecological tumors with better prognoses had higher baseline activation levels of CD4+ T cells compared to other solid tumors with poorer *** study elucidated the characteristics of different lymphocyte subsets in the peripheral blood of solid tumor patients. Further research revealed changes in the phenotypes of different lymphocyte subsets after ICIs treatment, with the activated phenotype of CD4+ T cells playing a crucial role in the antitumor effect. This lays the groundwork for further exploration of prognostic biomarkers and predictive models for cancer patients with immunotherapy.

关键词： immune checkpoint inhibitors lymphocyte subsets peripheral blood solid tumor

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：