Applications of fluorescence in situ hybridization (FISH) for translocation studies and biological dosimetry would benefit substantially from reliable and efficient automatic detection of metaphase chromosomes labeled...
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Applications of fluorescence in situ hybridization (FISH) for translocation studies and biological dosimetry would benefit substantially from reliable and efficient automatic detection of metaphase chromosomes labeled with fluorescent dyes. We replicated and evaluated a fluorescence metaphase finder previously developed at the Medical Research Council (MRC), Human Genetics Unit (Scotland) and at Lawrence Berkeley Laboratory (LBL; California). The MRC/LBL system seemed to detect nearly all of the metaphases on the test slides, but it presented an unacceptable number of false positives (about five false positives per one true positive). Furthermore, we determined that the system actually overcalled true detections by counting certain metaphase spreads twice (duplicates). Through modifications of the MRC/LBL system, we developed the Lawrence Livermore National Laboratory (LLNL) system, which minimizes the detection of duplicates, incorporates new detection features, uses a binary decision tree (BDT) for classification, and provides functionalities to improve scanning accuracy and improve the post-detection review. To test the new system, DAPI-stained preparations of metaphase chromosomes from blood lymphocytes of four unrelated donors were placed on slides in drops ranging from 7 mm to 20 mm in diameter. Drops contained between 5 and 200 scorable metaphases each. The LLNL system achieved ≈90% detection of non-duplicated metaphases as verified by an expert cytogeneticist, with typically less than one false positive per every one true positive detected. Cytometry 33:10–18, 1998. Published 1998 Wiley-Liss, Inc.
A parallel method developed for reconstructing images from holograms obtained with optical scanning holography is presented. A standard portable message-passing library, MPI or message-passing interface, is used to pr...
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A parallel method developed for reconstructing images from holograms obtained with optical scanning holography is presented. A standard portable message-passing library, MPI or message-passing interface, is used to provide the environment of parallel programming. The parallel method is implemented using a network of workstations. The process of reconstructing images with twin-image removal from holograms is performed in parallel with respect to different reconstruction planes of interest. This process contributes to possible faster three-dimensional object identification to be investigated in future research.
Despite the limits inherent within linearized frequency-domain ship motion and wave load computer codes, strip theory has been found to provide the design community with a fairly robust, practical design tool with rea...
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Despite the limits inherent within linearized frequency-domain ship motion and wave load computer codes, strip theory has been found to provide the design community with a fairly robust, practical design tool with reasonable accuracy for most conventional displacement monohulls. However, the advent of new design concepts including multi-hulls and application of new materials as well as the push to incorporate reliability methods within surface ship structural design criteria has highlighted the need for more rigorous methods of developing a lifetime load spectrum. In this paper, a multilevel computation system for predicting ship motions and wave loads, up through and including extreme sea conditions, is presented. This system includes a traditional strip theory approach and newly developed linear and nonlinear three-dimensional time-domain methods. The new nonlinear methods are currently in the process of being validated by the U.S. Navy. The status of the current development is presented. Sample numerical results from the new nonlinear methods are compared with both linear frequency domain predictions and model tests.
A formulation is presented for defining domains of mobility for a planar convex body moving with three degrees-of-freedom among convex planar obstacles. Applications included are determination of areas of a factory fl...
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It has been observed, in earlier computations of bifurcation diagrams for dissipative partial differential equations, that the use of certain explicit approximate inertial forms can give rise to numerical artifacts su...
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