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Self-assembly and recrystallization of bacterial S-layer proteins of Bacillus sphaericus and Bacillus thuringiensis on silicone, mica and quartz crystal supports

Self-assembly and recrystallization of bacterial S-layer pro...

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Annual International Conference of the IEEE engineering in Medicine and Biology Society (EMBC)

作者： Neda Habibi Federico Caneva Soumetz Massimo Giulianelli Laura Pastorino Oscar Herrera Francesca Sbrana Roberto Raiteri Carmelina Ruggiero Department of Communication Computer and System Sciences Nanobiotechnology and Medical Informatics Laboratory University of Genova Italy Consiglio Nazionale delle Ricerche Area di Ricerca di Napoli 1 Napoli Campania IT Biophysical and Electronic Engineering Department Genoa Italy

The Oriented architecture of macromolecules plays a critical role in many aspects of Nanobiotechnology such as in the development of biosensors. To this regard, S-layers which constitute the outermost cell envelope component of many prokaryotic organisms, represent unique self assembled systems with the capability to rearrange into monomolecular and oriented arrays. These properties can be exploited to promote their crystallization on surfaces (e.g. silicone) which is pivotal for the subsequent immobilization of macromolecules and development of new biosensors. In this work the crystallization of bacterial S-layers obtained from Bacillus thuringiensis and Bacillus sphaericus CCM2177 on silicone, mica and quartz crystal surfaces were investigated. The SDS page results of S-layers isolated from the above mentioned bacteria put in evidence that their molecular weight (MW) was around 120 KDa and, as reported in the literature, slightly higher for those extracted by Bacillus thuringiensis. In addition, results showed that S-layers isolated from Bacillus thuringiensis form large crystalline domains on mica after 5 min whereas those extracted from Bacillus sphaericus CCM 2177 form a compact monolayer on silicone after 2 h. Results in this work put in evidence the possibility to use these substrates for the fabrication of sensitive biosensors.

关键词： Proteins Microorganisms Crystallization Biosensors Silicon Surface treatment

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Hybrid uniform distribution of particle swarm optimizer

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IEICE Transactions on Fundamentals of Electronics, Communications and computer Sciences 2010年第10期E93-A卷 1782-1791页

作者： Zhang, Junqi Tan, Ying Ni, Lina Xie, Chen Tang, Zheng Department of Computer Science and Technology Key Laboratory of Embedded System and Service Computing Ministry of Education Qingdao China Tongji University Shanghai 200092 China Key Laboratory of Machine Perception Ministry of Education Qingdao China Department of Machine Intelligence School of Electronics Engineering and Computer Science Peking University Beijing 100871 China College of Info Sci and Engi Shandong University of Science and Technology Qingdao China Department of Intellectual Information Systems Engineering University of Toyama Toyama-shi 930-8555 Japan

Particle swarm optimizer (PSO) is a stochastic global optimization technique based on a social interaction metaphor. Because of the complexity, dynamics and randomness involved in PSO, it is hard to theoretically analyze the mechanism on which PSO depends. Statistical results have shown that the probability distribution of PSO is a truncated triangle, with uniform probability across the middle that decreases on the sides. The "truncated triangle" is also called the "Maya pyramid" by Kennedy. However, very little is known regarding the sampling distribution of PSO in itself. In this paper, we theoretically analyze the "Maya pyramid" without any assumption and derive its computational formula, which is actually a hybrid uniform distribution that looks like a trapezoid and conforms with the statistical results. Based on the derived density function of the hybrid uniform distribution, the search strategy of PSO is defined and quantified to characterize the mechanism of the search strategy in PSO. In order to show the significance of these definitions based, on the derived hybrid uniform distribution, the comparison between the defined search strategies of the classical, linear decreasing weight based PSO and the canonical constricted PSO suggested by Clerc is illustrated and elaborated. Copyright © 2010 The Institute of Electronics, Information and Communication Engineers.

关键词： Probability distributions

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Novel and Flexible Complex Coefficient Linear Phase IIR Filters for Communications

Novel and Flexible Complex Coefficient Linear Phase IIR Filt...

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IEEE Asia Pacific Conference on Circuits and systems

作者： Xiaofang Zhou Jinyuan Zhou Linkai Wang Chao Lu Gerald E. Sobelman State Key Laboratory of ASIC & System Fudan University Dept. of Electrical and Computer Engineering University of Minnesota

ISBN: (纸本)9781424474547

This paper presents a novel of Complex Coefficient Linear Phase (CCLP) Infinite Impulse Response (IIR) filter. The detailed algorithm is provided, and the linear phase feature of the CCLP is proved mathematically. The algorithm can be widely used in the IIR filters for communications. Its application includes interference cancellation filter, image reject filter, etc.

关键词： IIR Complex coefficient Linear phase Interference Cancellation Image reject

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Research of geometric computing and its theory

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Shanghai Jiaotong Daxue Xuebao/Journal of Shanghai Jiaotong University 2010年第3期44卷 407-412页

作者： He, Yuan-Jun Department of Computer Science and Engineering MOE-Microsoft Key Laboratory for Intelligent Computing and Intelligent System Shanghai Jiaotong University Shanghai 200240 China

A new geometric computing theory was proposed. On the definition level of geometric elements, using the Cartesian coordinates ideology as reference, 2D and 3D ″geometric algebra elements″ (or ″geometric elements″ for short, which could construct an upper-level element in the solving sequence) were constructed by geometry algebraization methods. On the processing level of geometries, geometric problems were solved with geometry methods, by which a geometric element solving sequence could be constructed. Directional property was introduced into geometries in this theory and geometries were represented in a unified format. They help to simplify the processing of finding the geometric element solving sequence for a geometry problem. The paper also tried to theoretically find out an integrated solution for geometry ambiguity issues, and established a unified, standardized geometry computing architecture. The Leibniz's mind-to process geometric objects with geometric language-was implemented in an indirect way!

关键词： Geometry

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Monte Carlo analysis of a low power domino gate under parameter fluctuation

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Journal of Semiconductors 2009年第12期30卷 113-117页

作者：汪金辉吴武臣宫娜侯立刚彭晓宏高大明 VLSI and System Laboratory Beijing University of Technology Department of Computer Science and Engineering State University of New York at Buffalo

Using the multiple-parameter Monte Carlo method, the effectiveness of the dual threshold voltage technique （DTV） in low power domino logic design is analyzed. Simulation results indicate that under significant temperature and process fluctuations, DTV is still highly effective in reducing the total leakage and active power consumption for domino gates with speed loss. Also, regarding power and delay characteristics, different structure domino gates with DTV have different robustness against temperature and process fluctuation.

关键词： domino gate temperature and process fluctuation effectiveness

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A methodology for power quality evaluation in distribution network with distributed generation

A methodology for power quality evaluation in distribution n...

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International Conference on Critical Infrastructures, CRIS

作者： Gengyin Li Zhe Zhang Xiang Li Song Wang Ming Zhou Key Laboratory of Power System Protection and Dynamic Security Monitoring and Control under Ministry of Education North China Electric Power University Beijing China Department of Electrical Engineering Tsinghua University Haidian Beijing China School of Electrical and Computer Engineering North China Electric Power University Beijing China

ISBN: (纸本)9781424480807

The issue of power quality in the distribution system is always in sense of the concern. With the high penetration of distributed generation (DG) currently, it tends to be more complicated. This paper presents a new approach to evaluate impacts of the DG on power quality of the distribution network. In the process an objective function will be modeled, which will be solved by the optimization toolbox, in order to calculate the result for the evaluating grade. Furthermore, the results of the overall evaluation will be given via the eigenvalue weighted method. The power quality ranking of each feeder with the distributed generators in the distribution network will be apparent with the given weight of influence caused by DGs divided in forms of energy resource.

关键词： Power quality Generators Indexes Distributed power generation Eigenvalues and eigenfunctions Optimization Voltage fluctuations

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Computationally efficient, approximate moving horizon state estimation for nonlinear systems

Computationally efficient, approximate moving horizon state ...

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作者： Alessandri, A. Baglietto, M. Battistelli, G. Zavala, V. Department of Production Engineering Thermoenergetics and Mathematical Models DIPTEM University of Genoa P.le Kennedy Pad. D 16129 Genova Italy Department of Communications Computer and System Sciences DIST University of Genoa Via Opera Pia 13 16145 Genova Italy Dipartimento di Sistemi e Informatica Università degli Studi di Firenze Via di S. Marta 3 50139 Firenze Italy Mathematics and Computer Science Division Argonne National Laboratory 9700 South Cass Ave Bldg. 240 Argonne IL 60439 United States

ISBN: (纸本)9783902661807

Moving horizon estimation for discrete-time nonlinear systems is addressed by using fast optimization algorithms for which stability results under general conditions are ensured. The solution of the on-line moving horizon estimation problem is obtained by using the sampling time to solve a reference problem with model-predicted measurements while waiting for the next measurement. In order to correct the resulting solution, a quick nonlinear programming sensitivity calculation is accomplished as soon as the new measurement becomes available. The stability properties of such moving horizon estimation algorithm is proved under general conditions, which make the overall approach suitable for real settings with strong nonlinearities. © 2010 IFAC.

关键词： Nonlinear programming

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Spatio-temporal localization of focal epileptic sources from intracranial electrocortical recordings using an independent component analysis (ICA) algorithm

Spatio-temporal localization of focal epileptic sources from...

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Iranian Conference of Biomedical engineering (ICBME)

作者： Mohamed Amin Latif Abbas Babajani-Feremi Daniel Barkmeier Jeffery A. Leob Hamid Soltanian-Zadeh Image Analysis Laboratory Radiology Department Henry Ford Health System Detroit MI USA The Center of Molecular Medicine and Genetics Wayne State University School of Medicine Detroit MI USA CIPCE Electrical and Computer Engineering Department University of Tehran Tehran Iran

The gold standard for the localization of epileptic activities in the cerebral cortex is intracranial electrocorticography (ECoG) electrodes placed directly on the brain surface. However, it has limitations in being able to localize deep brain epileptic sources. As a means to improve the localization of epileptic activities from these subdural electrical recordings, we developed a simple source monitoring system to detect and localize these deep sources. The method is based on an independent component analysis (ICA) algorithm known as joint approximate diagonalization of eigen-matrices (JADE). This method was tested on patients with neocortical epileptic foci focusing on the interictal spikes from ECoG signals. The proposed method localizes the interictal epileptic discharges to both superficial as well as deep regions of the human neocortex and has the potential to improve the localization and surgical outcomes of patients with medically refractory epilepsy undergoing surgical resections.

关键词： Biological system modeling Epilepsy Surgery Electrodes Magnetic resonance imaging Signal to noise ratio Brain modeling

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Optimal decisions of closed-loop supply chain considering substitution in new and old product

Optimal decisions of closed-loop supply chain considering su...

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作者： Zhou, Yancong Sun, Junqing Li, Weiwei School of Information Engineering Tianjin University of Commerce Tianjin 300134 China Key Laboratory of Computer Vision and System Tianjin University of Technology Tianjin 300191 China General Courses Department Academy of Military Transportation Tianjin 300161 China

ISBN: (纸本)9787894631046

In a closed-loop supply chain system with new and old product, remanufacturing and substitution was considered in the problem. Before random demand and random return are realized, the manufacutring quantities of new and old product, and remanufacturing quantity of used product need to be decided. Firstly, the closed loop system model with substitution and random return was proposed, and the optimal decsion problem was given. Secondly, the problem was translated into other optimal problem, the existence and uniqueness of optimal solution were proved, and the optimal decision structure was given.

关键词： Supply chains

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Electrical Breakdown Properties of Oil-Paper Insulation under AC-DC Combined Voltages

Electrical Breakdown Properties of Oil-Paper Insulation unde...

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IEEE International Power Modulator and Hogh Voltage Conference

作者： Yan Wang Jian Li Youyuan Wang Stanislaw Grzybowski State Key Laboratory of Power Transmission Equipment and System Security and New Technology College of Electrical Engineering Chongqing University High Voltage Laboratory Department of Electrical and Computer Engineering Mississippi State University

ISBN: (纸本)9781424471317

Oil-impregnated paper is a major type of insulation in oil-filled power transformers. In converter transformers, windings connected with converter valves have to withstand AC and DC combined voltages. This paper presented the experimental research on electrical breakdown properties of oilpaper insulation under AC-DC combined voltages. Electrical breakdown strength of oil-paper insulation specimens was measured through short-time tests under AC, DC, and AC-DC combined voltages. The combined voltages consisted of two different ratios of the AC to DC voltages. Through the constant-stress tests, the times-to-breakdown of oil-paper specimens were measured. Weibull parameters of the times-to-breakdown of specimens were estimated and the inverse power and exponential failure models of the specimens were presented.

关键词： Converter transformer Oil-paper insulation AC-DC combined voltage Electrical breakdown Failure model

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