An approach to multi-fueled DNA nano-robotics is described. We propose three types of driving force (i.e., fuel for DNA nano-robots): thermal fuel, pH fuel, and light fuel. The thermal fuel controls the hybridization ...
An approach to multi-fueled DNA nano-robotics is described. We propose three types of driving force (i.e., fuel for DNA nano-robots): thermal fuel, pH fuel, and light fuel. The thermal fuel controls the hybridization of DNA molecules around the melting temperature. The pH fuel controls the hybridization of the so-called i-motif by changing the pH condition. The light fuel controls the hybridization of DNA oligomers that are intercalated with azobenzene by irradiation with UV or visible light. These three fuels are not mutually exclusive. However, experimental conditions for the fueling of DNA nano-robots show efficacy. Concrete ideas for using these three fuel types are proposed and discussed. In addition, the results of calibration experiments and preliminary results for refining pH fuel sequence are also shown.
In recent years,large-scale sequencing of complete genomes has fueled the discovery of single-nucleotide polymorphisms(SNPs),which have been the representatives of genetic variants associated with susceptibility to va...
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In recent years,large-scale sequencing of complete genomes has fueled the discovery of single-nucleotide polymorphisms(SNPs),which have been the representatives of genetic variants associated with susceptibility to various common diseases and response to various *** a number of methods have been proposed for SNPs mapping and typing[1],most of
作者：
T.SeinoS.NishizawaN.TeramaeDepartment of Chemistry
Graduate School of Science Tohoku University and Core Research for Evolution Science and Technology (CREST) Japan Science and Technology Agency Aoba-ku Sendai 980-8578 Japan
Pulse shaping and phase control of femtosecond pulses are shown to have effect on the dielectric breakdown of sapphire. The influence of a pulse tilt on a dielectric breakdown is demonstrated. Three-dimensional struct...
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Pulse shaping and phase control of femtosecond pulses are shown to have effect on the dielectric breakdown of sapphire. The influence of a pulse tilt on a dielectric breakdown is demonstrated. Three-dimensional structuring of transparent dielectric and semiconducting materials at tight focusing is discussed.
Bidirectional coincidence counting of correlated photons was adopted to determine the detection efficiency of single-photon detectors operating at 1550nm without comparing to a reference standard. A self-calibration m...
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ISBN:
(纸本)1424411742
Bidirectional coincidence counting of correlated photons was adopted to determine the detection efficiency of single-photon detectors operating at 1550nm without comparing to a reference standard. A self-calibration method best suitable for single-photon detectors was established.
Bcl-2 is a member of the large Bcl-2 family and protects cells from apoptosis. Ultraviolet B (UVB) irradiation induces apoptosis of keratinocytes that is known as “sunburn cells.” Previously we reported that UVB irr...
Bcl-2 is a member of the large Bcl-2 family and protects cells from apoptosis. Ultraviolet B (UVB) irradiation induces apoptosis of keratinocytes that is known as “sunburn cells.” Previously we reported that UVB irradiation induces apoptosis accompanied by sequential activation of caspase 8, 3 and 1 in keratinocytes, and that the process is inhibited by various caspase inhibitors. Using bcl-2–expressing adenovirus vector we investigated the effect of Bcl-2 on UVB-induced apoptosis. Adenovirus vector efficiently introduced bcl-2 gene in cultured normal mouse keratinocytes (NMK cells); almost all NMK cells (1 × 10 6 ) were transfected at 1 × 10 8 plaque-forming unit (PFU)/mL. Bcl-2–transfected NMK cells were significantly resistant to UVB-induced apoptosis with the suppressive effect dependent on the Bcl-2 expression level. Following UVB irradiation caspase 8, 3 and 9 activities were stimulated in NMK cells, whereas in bcl-2–transfected cells only caspase 8, but not caspase 3 or 9, activity was stimulated. In order to investigate the effect of Bcl-2 in vivo topical application of Ad-bcl-2 on tape-stripped mouse skin was performed. Following the application Bcl-2 was efficiently overexpressed in almost all viable keratinocytes. The expression was transient with the maximal expression of Bcl-2 on the first day following the application of 1 × 10 9 PFU in 200 μL. The introduced Bcl-2 remained at least for 6 days. UVB irradiation (1250 J/m 2 ) induced apoptosis within 12 h and the maximal effect was observed at 24 h in control mouse skin. Both bcl-2–transfected and topical caspase 3 inhibitor-treated mice skin were resistant to UVB-induced apoptosis. The suppressive effect of Bcl-2 was more potent than that of caspase 3 inhibitor application. Topical application of empty adenovirus vector alone had no effect on Bcl-2 expression or UVB-induced apoptosis. These results indicate that adenovirus vector is an efficient gene delivery system into keratinocytes and that Bcl-2 is
A novel optical buffer memory with a shift register function using a two-dimensional array of polarization bistable VCSELs is proposed. 1-bit optical buffering, which is an essential part of the memory, has been exper...
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ISBN:
(纸本)1557528136
A novel optical buffer memory with a shift register function using a two-dimensional array of polarization bistable VCSELs is proposed. 1-bit optical buffering, which is an essential part of the memory, has been experimentally demonstrated.
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