We recently reported that SPIN90 is able to bind with several proteins involved in regulating actin cytoskeleton networks, including dynamin, WASP, beta PIX, and Nck. Based on these findings, we investigated how SPIN9...
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We recently reported that SPIN90 is able to bind with several proteins involved in regulating actin cytoskeleton networks, including dynamin, WASP, beta PIX, and Nck. Based on these findings, we investigated how SPIN90 regulates the actin cytoskeleton and promotes actin assembly. This study demonstrated that aluminium fluoride-induced localization of SPIN90 to lamellipodia requires amino acids 582-722 at the SPIN90 C-terminus, which is also essential for F-actin binding and Arp2/3 complex mediated polymerization of actin into branched actin filaments. Furthermore, after deletion of the F-actin binding region ( 582-722 SPIN90) failed to localize at the membrane edge and was unable to promote lamellipodia formation, suggesting that the F-actin binding region in the SPIN90 C-terminus is essential for the formation of branched actin networks and regulation of the actin cytoskeleton at the leading edge of cells.
Au-activated InAs nanowires were grown on Si substrates by solid source molecular beam epitaxy (SSMBE). Epitaxial growth of InAs nanowires turned out to be very sensitive to the surface condition of the Si substrates....
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Au-activated InAs nanowires were grown on Si substrates by solid source molecular beam epitaxy (SSMBE). Epitaxial growth of InAs nanowires turned out to be very sensitive to the surface condition of the Si substrates. InAs nanowires having a < 111 > growth direction, a high crystalline quality and a high aspect ratio over 300 with a uniform lateral size along the growth direction were grown using a high-temperature pre-annealing process in the MBE growth chamber to remove residual oxides from the surface of the Si substrates.
Temperature-dependent bandwidths and responsivities of uni-traveling-carrier (UTC) and modified uni-traveling-carrier (MUTC) photodiodes were theoretically and experimentally investigated. Theoretical and experimental...
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Temperature-dependent bandwidths and responsivities of uni-traveling-carrier (UTC) and modified uni-traveling-carrier (MUTC) photodiodes were theoretically and experimentally investigated. Theoretical and experimental responsivities of the UTC and MUTC photodiodes were improved as the operating temperature increased. On the other hand, each type of photodiode exhibited different temperature-dependent bandwidth characteristics depending on the thickness of the undoped photoabsorption layer. The bandwidth characteristics of the MUTC photodiode showed weak temperature dependence, which makes it suitable for application to outdoor optoelectronic converters.
An all-optical frequency up-conversion technique based on a four-wave mixing (FWM) effect in semiconductor optical amplifiers (SOAs) was utilized for conversion of an optical intermediate frequency (IF) signal (2.5 GH...
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ISBN:
(纸本)9781424406876
An all-optical frequency up-conversion technique based on a four-wave mixing (FWM) effect in semiconductor optical amplifiers (SOAs) was utilized for conversion of an optical intermediate frequency (IF) signal (2.5 GHz) to an optical radio frequency (RF) signal (40 GHz) by mixing with an optical local oscillator (LO) signal (37.5 GHz). This scheme provides positive conversion efficiency of 5.77 dB for the optical IF power of -22 dBm and the optical LO power of -13 dBm. The conversion efficiency was almost flat for the LO frequency range from 20 GHz to 47.5 GHz. The phase noise at the LO frequency of 37.5 GHz was -84.5 dBc/Hz (at 10 kHz offset) after the frequency up-conversion.
SPIN90, a 90-kDa Nck-interacting protein with a SH3 domain, plays a role in sarcomere formation and myofibril assembly, and its phosphorylation is modulated by cell adhesion and Erk activation. Here we demonstrate tha...
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SPIN90, a 90-kDa Nck-interacting protein with a SH3 domain, plays a role in sarcomere formation and myofibril assembly, and its phosphorylation is modulated by cell adhesion and Erk activation. Here we demonstrate that SPIN90 participates in receptor-mediated endocytic pathway in fibroblasts. We identified syndapin (synaptic dynamin-binding protein) as a SPIN90 interacting protein using yeast two-hybrid screening. SPIN90 directly binds the SH3 domain of syndapin via its proline rich domain in vitro and in vivo and also associates with clathrin. Over-expression of SPIN90-full length in COS-7 cells inhibited transferrin uptake, a marker of endocytosis. Interestingly, SPIN90-PRD, a syndapin-binding domain, significantly inhibited endocytosis, and the inhibition was reversed by co-expression of syndapin. Depleting SPIN90 through antibody microinjection or Knocking it down using siRNAs also significantly inhibited transferrin internalization. Moreover, early endosomal marker proteins (EEA1 and Rab5) appeared to closely associate or partially co-localize with SPIN90 in endosomes and an internalized FITC-dextran and Texas Red-EGF were found on the endosomes in association with SPIN90. Time-lapse video showed that GFP-SPIN90 travels with moving vesicles within living cells. Taken together, these findings suggest that SPIN90 is implicated in receptor-mediated endocytic pathway in fibroblasts.
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