A spherical microcavity array (SMCA) chip is developed for performing digital PCR (dPCR) in a very simple and inexpensive format. The spherical geometry of the microchambers in the SMCA chip provides an energy advanta...
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ISBN:
(纸本)9780979806490
A spherical microcavity array (SMCA) chip is developed for performing digital PCR (dPCR) in a very simple and inexpensive format. The spherical geometry of the microchambers in the SMCA chip provides an energy advantage for the stable confinement of partitioned droplets during PCR thermal cycling. Meanwhile, the device is powered by pre-degassing of its PDMS substrate that eliminates the need for complex mechanical pumps, valves or external tubing. Thus, it offers an easy-to-use and inexpensive way to perform dPCR in resource-limited areas.
A new quadrature signal generator based on Direct Digital Synthesis (DDS) algorithm for digital demodulation of MEMS gyroscope was proposed in this paper. To avoid quantization error, the dual-accumulator with signal-...
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A new quadrature signal generator based on Direct Digital Synthesis (DDS) algorithm for digital demodulation of MEMS gyroscope was proposed in this paper. To avoid quantization error, the dual-accumulator with signal-...
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A novel dielectrophoresis(DEP) chip which deposited array of parallel electrodes was used to separate and concentrate cells based on the principle and theory of dielectrophoresis. Pitch gradient here was left between ...
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A novel dielectrophoresis(DEP) chip which deposited array of parallel electrodes was used to separate and concentrate cells based on the principle and theory of dielectrophoresis. Pitch gradient here was left between the electrode gap, which amplified the Range of DEP force. The chip can not only reduce the magnitude of the applied AC signal, but also can trap cells on the electrode by DEP force effectively. The RBC and HepG2 were separated under the buffer conductivity of 600 uS/cm and separating voltage of 5V, and capture efficiencies of HepG2 under 300 kHz and 400 kHz, were 82.5% and 85.8% respectively. It was showed that Blood Cells and HepG2 were separated and HepG2 Cells were concentrated effectively in the microfluidic system. It was illuminated that the chip can be used to separate cells and concentrate cells. And it can be applied in the DEP field and its system. Thus it was of great important in the application field and had a pelenty of practical values.
A microfluidic chip with in-situ prepared chitosan membrane immobilized with Glucose Oxidase was developed as glucose oxidase reactor to detect human serum. The chitosan was prepared in-situ in the micro-channel as th...
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