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QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy

arXiv

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arXiv 2021年

作者： Nelson, Glyn Boehm, Ulrike Bagley, Steve Bajcsy, Peter Bischof, Johanna Brown, Claire M. Dauphin, Aurélien Dobbie, Ian M. Eriksson, John E. Faklaris, Orestis Fernandez-Rodriguez, Julia Ferrand, Alexia Gelman, Laurent Gheisari, Ali Hartmann, Hella Kukat, Christian Laude, Alex Mitkovski, Miso Munck, Sebastian North, Alison J. Rasse, Tobias M. Resch-Genger, Ute Schuetz, Lucas C. Seitz, Arne Strambio-De-Castillia, Caterina Swedlow, Jason R. Alexopoulos, Ioannis Aumayr, Karin Avilov, Sergiy Bakker, Gert-Jan Bammann, Rodrigo R. Bassi, Andrea Beckert, Hannes Beer, Sebastian Belyaev, Yury Bierwagen, Jakob Birngruber, Konstantin A. Bosch, Manel Breitlow, Juergen Cameron, Lisa A. Chalfoun, Joe Chambers, James J. Chen, Chieh-Li Conde-Sousa, Eduardo Corbett, Alexander D. Cordelieres, Fabrice P. Nery, Elaine Del Dietzel, Ralf Eismann, Frank Fazeli, Elnaz Felscher, Andreas Fried, Hans Gaudreault, Nathalie Goh, Wah Ing Guilbert, Thomas Hadleigh, Roland Hemmerich, Peter Holst, Gerhard A. Itano, Michelle S. Jaffe, Claudia B. Jambor, Helena K. Jarvis, Stuart C. Keppler, Antje Kirchenbuechler, David Kirchner, Marcel Kobayashi, Norio Krens, Gabriel Kunis, Susanne Lacoste, Judith Marcello, Marco Martins, Gabriel G. Metcalf, Daniel J. Mitchell, Claire A. Moore, Joshua Mueller, Tobias Nelson, Michael S. Ogg, Stephen Onami, Shuichi Palmer, Alexandra L. Paul-Gilloteaux, Perrine Pimentel, Jaime A. Plantard, Laure Podder, Santosh Rexhepaj, Elton Royon, Arnaud Saari, Markku A. Schapman, Damien Schoonderwoert, Vincent Schroth-Diez, Britta Schwartz, Stanley Shaw, Michael Spitaler, Martin Stoeckl, Martin T. Sudar, Damir Teillon, Jeremie Terjung, Stefan Thuenauer, Roland Wilms, Christian D. Wright, Graham D. Nitschke, Roland Bioimaging Unit Newcastle University Newcastle upon TyneNE4 5PL United Kingdom Janelia Research Campus Howard Hughes Medical Institute AshburnVA20147 United States Visualisation Irradiation & Analysis Cancer Research UK Manchester Institute Alderley Park Macclesfield United Kingdom National Institute of Standards and Technology GaithersburgMD20899 United States Euro-BioImaging Heidelberg69117 Germany McGill University MontrealQCH3G 0B1 Canada Unité Génétique et Biologie du Développement U934 PICT-IBiSA Institut Curie/Inserm CNRS PSL Research University Paris75005 France Department of Biochemistry University of Oxford Oxford OxonOX1 3QU United Kingdom Turku Bioscience Centre Euro-Bioimaging ERIC Turku20520 Finland Biocampus CNRS UAR 3426 Montpellier34293 France Centre for Cellular Imaging Sahlgrenska Academy University of Gothenburg Gothenburg40530 Sweden Imaging Core Facility Biozentrum University of Basel Basel4056 Switzerland Friedrich Miescher Institute for Biomedical Research Basel4058 Switzerland Light Microscopy Facility CMCB Technology Platform TU Dresden Dresden01307 Germany FACS & Imaging Core Facility Max Planck Institute for Biology of Ageing Cologne50931 Germany Light Microscopy Facility Max Planck Institute of Experimental Medicine Goettingen37075 Germany VIB BioImaging Core VIB-KU Leuven Center for Brain and Disease Research KU Leuven Department for Neuroscience Flanders Leuven3000 Belgium The Rockefeller University New YorkNY10065 United States Scientific Service Group Microscopy Max Planck Institute for Heart and Lung Research Bad Nauheim61231 Germany Division Biophotonics Federal Institute for Materials Research and Testing Berlin12489 Germany European Molecular Biology Laboratory Advanced Light Microscopy Facility Heidelberg69117 Germany Faculty of Life Sciences Ecole Polytechnique Fédérale de Lausanne Vaud Lausanne1015 Switzerland Program in Molecular Medicine University of Massachusetts Medica

A modern day light microscope has evolved from a tool devoted to making primarily empirical observations to what is now a sophisticated, quantitative device that is an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use in capturing and quantifying scientific phenomena, neither a thorough understanding of the principles underlying quantitative imaging techniques nor appropriate knowledge of how to calibrate, operate and maintain microscopes can be taken for granted. This is clearly demonstrated by the well-documented and widespread difficulties that are routinely encountered in evaluating acquired data and reproducing scientific experiments. Indeed, studies have shown that more than 70% of researchers have tried and failed to repeat another scientist’s experiments, while more than half have even failed to reproduce their own experiments1. One factor behind the reproducibility crisis of experiments published in scientific journals is the frequent underreporting of imaging methods caused by a lack of awareness and/or a lack of knowledge of the applied technique2. Whereas quality control procedures for some methods used in biomedical research, such as genomics (e.g., DNA sequencing, RNA-seq) or cytometry, have been introduced (e.g. ENCODE3), this issue has not been tackled for optical microscopy instrumentation and images. Although many calibration standards and protocols have been published, there is a lack of awareness and agreement on common standards and guidelines for quality assessment and reproducibility4. In April 2020, the QUality Assessment and REProducibility for Instruments and Images in Light Microscopy (QUAREP-LiMi) initiative5 was formed. This initiative comprises imaging scientists from academia and industry who share a common interest in achieving a better understanding of the performance and limitations of microscopes and improved qualit

关键词： DNA sequences

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High‐Resolution Electrochemical Mapping of the Hydrogen Evolution Reaction on Transition‐Metal Dichalcogenide Nanosheets

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Angewandte Chemie 2019年第9期132卷

作者： Yasufumi Takahashi Yu Kobayashi Ziqian Wang Yoshikazu Ito Masato Ota Hiroki Ida Akichika Kumatani Keisuke Miyazawa Takeshi Fujita Hitoshi Shiku Yuri E. Korchev Yasumitsu Miyata Takeshi Fukuma Mingwei Chen Tomokazu Matsue WPI Nano Life Science Institute (NanoLSI WPI) Kanazawa University Kakuma-machi Kanazawa Ishikawa 920-1192 Japan Precursory Research for Embryonic Science and Technology (PRESTO) (Japan) Science and Technology Agency (JST) Saitama 332-0012 Japan Department of Physics Tokyo Metropolitan University Hachioji Tokyo 192-0397 Japan Department of Materials Science and Engineering Johns Hopkins University Baltimore MD 21218 USA Institute of Applied Physics Graduate School of Pure and Applied Sciences University of Tsukuba Tsukuba Ibaraki 305-8573 Japan Graduate School of Environmental Studies Tohoku University 6-6-11-604 Aramaki Aoba Aoba-ku Sendai 980-8579 Japan WPI-Advanced Institute for Materials Research (AIMR) Tohoku University 2-1-1-509 Katahira Aoba-ku Sendai 980-8577 Japan School of Environmental Science and Engineering Kochi University of Technology Kochi 782-8502 Japan Department of Applied Chemistry Graduate School of Engineering Tohoku University Sendai 980-8579 Japan Department of Medicine Imperial College London London W12 0NN UK Core Research for Evolutional Science and Technology (CREST) (Japan) Science and Technology Agency (JST) Saitama 332-0012 Japan

High‐resolution scanning electrochemical cell microscopy (SECCM) is used to image and quantitatively analyze the hydrogen evolution reaction (HER) catalytically active sites of 1H‐MoS 2 nanosheets, MoS 2 , and WS 2 heteronanosheets. Using a 20 nm radius nanopipette and hopping mode scanning, the resolution of SECCM was beyond the optical microscopy limit and visualized a small triangular MoS 2 nanosheet with a side length of ca. 130 nm. The electrochemical cell provides local cyclic voltammograms with a nanoscale spatial resolution for visualizing HER active sites as electrochemical images. The HER activity difference of edge, terrace, and heterojunction of MoS 2 and WS 2 were revealed. The SECCM imaging directly visualized the relationship of HER activity and number of MoS 2 nanosheet layers and unveiled the heterogeneous aging state of MoS 2 nanosheets. SECCM can be used for improving local HER activities by producing sulfur vacancies using electrochemical reaction at the selected region.

关键词： Elektrochemie Wasserstoffentwicklung MoS2 Elektrochemische Rastermikroskopie

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Quantifying antiviral activity optimizes drug combinations against hepatitis C virus infection (vol 114, pg 1922, 2017)

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PROCEEDINGS OF THE NATIONAL ACADEMY OF scienceS OF THE UNITED STATES OF AMERICA 2017年第12期114卷 E2540-E2541页

作者： Koizumi, Yoshiki Ohashi, Hirofumi Nakajima, Syo Tanaka, Yasuhito Wakita, Takaji Perelson, Alan S. Iwami, Shingo Watashi, Koichi School of Medicine College of Medical Pharmaceutical and Health Sciences Kanazawa University Ishikawa 920-8640 Japan. Department of Virology II National Institute of Infectious Diseases Tokyo 162-8640 Japan. Department of Applied Biological Sciences Faculty of Science and Technology Tokyo University of Sciences Chiba 278-8510 Japan. Department of Virology and Liver Unit Nagoya City University Graduate School of Medicinal Sciences Nagoya 467-8601 Japan. Theoretical Biology and Biophysics Group Los Alamos National Laboratory Los Alamos NM 87545. Mathematical Biology Laboratory Department of Biology Faculty of Sciences Kyushu University Fukuoka 812-8581 Japan siwami@*** kwatashi@nih.go.jp. Precursory Research for Embryonic Science and Technology (PRESTO) Japan Science and Technology Agency Saitama 332-0012 Japan. Core Research for Evolutional Science and Technology (CREST) Japan Science and Technology Agency Saitama 332-0012 Japan. Department of Virology II National Institute of Infectious Diseases Tokyo 162-8640 Japan siwami@*** kwatashi@nih.go.jp.

With the introduction of direct-acting antivirals (DAAs), treatment against hepatitis C virus (HCV) has significantly improved. To manage and control this worldwide infectious disease better, the "best" multidrug treatment is demanded based on scientific evidence. However, there is no method available that systematically quantifies and compares the antiviral efficacy and drug-resistance profiles of drug combinations. Based on experimental anti-HCV profiles in a cell culture system, we quantified the instantaneous inhibitory potential (IIP), which is the logarithm of the reduction in viral replication events, for both single drugs and multiple-drug combinations. From the calculated IIP of 15 anti-HCV drugs from different classes [telaprevir, danoprevir, asunaprevir, simeprevir, sofosbuvir (SOF), VX-222, dasabuvir, nesbuvir, tegobuvir, daclatasvir, ledipasvir, IFN-α, IFN-λ1, cyclosporin A, and SCY-635], we found that the nucleoside polymerase inhibitor SOF had one of the largest potentials to inhibit viral replication events. We also compared intrinsic antiviral activities of a panel of drug combinations. Our quantification analysis clearly indicated an advantage of triple-DAA treatments over double-DAA treatments, with triple-DAA treatments showing enhanced antiviral activity and a significantly lower probability for drug resistance to emerge at clinically relevant drug concentrations. Our framework provides quantitative information to consider in designing multidrug strategies before costly clinical trials.

关键词： HCV antiviral instantaneous inhibitory potential mathematical model replicon

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Erratum: 'The JCMT BISTRO Survey: Magnetic Fields Associated with a Network of Filaments in NGC 1333' (2020, ApJ, 899, 28)

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The Astrophysical Journal 2021年第1期911卷

作者： Yasuo Doi Tetsuo Hasegawa Ray S. Furuya Simon Coudé Charles L. H. Hull Doris Arzoumanian Pierre Bastien Michael Chun-Yuan Chen James Di Francesco Rachel Friesen Martin Houde Shu-ichiro Inutsuka Steve Mairs Masafumi Matsumura Takashi Onaka Sarah Sadavoy Yoshito Shimajiri Mehrnoosh Tahani Kohji Tomisaka Chakali Eswaraiah Patrick M. Koch Kate Pattle Chang Won Lee Motohide Tamura David Berry Tao-Chung Ching Jihye Hwang Woojin Kwon Archana Soam Jia-Wei Wang Shih-Ping Lai Keping Qiu Derek Ward-Thompson Do-Young Byun Huei-Ru Vivien Chen Wen Ping Chen Zhiwei Chen Jungyeon Cho Minho Choi Yunhee Choi Antonio Chrysostomou Eun Jung Chung Pham Ngoc Diep Hao-Yuan Duan Lapo Fanciullo Jason Fiege Erica Franzmann Per Friberg Gary Fuller Tim Gledhill Sarah F. Graves Jane S. Greaves Matt J. Griffin Qilao Gu Ilseung Han Jennifer Hatchell Saeko S. Hayashi Thiem Hoang Tsuyoshi Inoue Kazunari Iwasaki Il-Gyo Jeong Doug Johnstone Yoshihiro Kanamori Ji-hyun Kang Miju Kang Sung-ju Kang Akimasa Kataoka Koji S. Kawabata Francisca Kemper Gwanjeong Kim Jongsoo Kim Kee-Tae Kim Kyoung Hee Kim Mi-Ryang Kim Shinyoung Kim Jason M. Kirk Masato I. N. Kobayashi Vera Konyves Takayoshi Kusune Jungmi Kwon Kevin Lacaille Chi-Yan Law Chin-Fei Lee Hyeseung Lee Jeong-Eun Lee Sang-Sung Lee Yong-Hee Lee Dalei Li Di Li Hua-bai Li Hong-Li Liu Junhao Liu Sheng-Yuan Liu Tie Liu Ilse de Looze A-Ran Lyo Brenda C. Matthews Gerald H. Moriarty-Schieven Tetsuya Nagata Fumitaka Nakamura Hiroyuki Nakanishi Nagayoshi Ohashi Geumsook Park Harriet Parsons Nicolas Peretto Tae-Soo Pyo Lei Qian Ramprasad Rao Mark G. Rawlings Brendan Retter John Richer Andrew Rigby Hiro Saito Giorgio Savini Anna M. M. Scaife Masumichi Seta Hiroko Shinnaga Ya-Wen Tang Yusuke Tsukamoto Serena Viti Hongchi Wang Anthony P. Whitworth Hsi-Wei Yen Hyunju Yoo Jinghua Yuan Hyeong-Sik Yun Tetsuya Zenko Chuan-Peng Zhang Guoyin Zhang Yapeng Zhang Jianjun Zhou Lei Zhu Philippe André C. Darren Dowell Stewart P. S. Eyres Sam Falle Sven van Loo Jean-François Robitaille Department of Earth Science and Astronomy Graduate School of Arts and Sciences The University of Tokyo 3-8-1 Komaba Meguro Tokyo 153-8902 Japan doi@ea.c.u-tokyo.ac.jp National Astronomical Observatory of Japan National Institutes of Natural Sciences Osawa Mitaka Tokyo 181-8588 Japan Institute of Liberal Arts and Sciences Tokushima University Minami Jousanajima-machi 1-1 Tokushima 770-8502 Japan SOFIA Science Center Universities Space Research Association NASA Ames Research Center M.S. N232-12 Moffett Field CA 94035 USA Centre de Recherche en Astrophysique du Québec (CRAQ) Université de Montréal Département de Physique C.P. 6128 Succ. Centre-ville Montréal QC H3C 3J7 Canada National Astronomical Observatory of Japan NAOJ Chile Alonso de Córdova 3788 Office 61B 7630422 Vitacura Santiago Chile Joint ALMA Observatory Alonso de Córdova 3107 Vitacura Santiago Chile Instituto de Astrofísica e Ciências do Espaço Universidade do Porto CAUP Rua das Estrelas PT4150-762 Porto Portugal Department of Physics Graduate School of Science Nagoya University Furo-cho Chikusa-ku Nagoya 464-8602 Japan Institut de Recherche sur les Exoplanètes (iREx) Université de Montréal Département de Physique C.P. 6128 Succ. Centre-ville Montréal QC H3C 3J7 Canada Department of Physics and Astronomy University of Victoria Victoria BC V8P 1A1 Canada Herzberg Astronomy and Astrophysics Research Centre National Research Council of Canada 5071 West Saanich Rd. Victoria BC V9E 2E7 Canada National Radio Astronomy Observatory 520 Edgemont Rd. Charlottesville VA 22903 USA Department of Physics and Astronomy The University of Western Ontario 1151 Richmond Street London ON N6A 3K7 Canada East Asian Observatory 660 N. A'ohōkū Place University Park Hilo HI 96720 USA Faculty of Education Kagawa University Saiwai-cho 1-1 Takamatsu Kagawa 760-8522 Japan Department of Astronomy Graduate School of Science The University of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo 113-0033 Japan Depar

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Helicity-dependent terahertz emission spectroscopy of topological insulator Sb2Te3 thin films

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Physical Review B 2017年第19期96卷 195407-195407页

作者： Chien-Ming Tu Yi-Cheng Chen Ping Huang Pei-Yu Chuang Ming-Yu Lin Cheng-Maw Cheng Jiunn-Yuan Lin Jenh-Yih Juang Kaung-Hsiung Wu Jung-Chun A. Huang Way-Faung Pong Takayoshi Kobayashi Chih-Wei Luo Department of Electrophysics National Chiao Tung University Hsinchu Taiwan 300 Republic of China Department of Physics Tamkang University New Taipei City 25137 Taiwan Republic of China Department of Physics Lund University P.O. Box 118 SE-221 00 Lund Sweden Department of Physics and Advanced Optoelectonic Technology Center National Cheng Kung University Tainan 701 Taiwan Republic of China National Synchrotron Radiation Research Center Hsinchu Taiwan 300 Republic of China Institute of Physics National Chiao Tung University Hsinchu Taiwan 300 Republic of China Taiwan Consortium of Emergent Crystalline Materials Ministry of Science and Technology Taipei 10601 Taiwan Republic of China Advanced Ultrafast Laser Research Center and Department of Engineering Science The University of Electro-Communications Chofugaoka 1-5-1 Chofu Tokyo 182-8585 Japan Japan Science and Technology Agency Core Research for Evolutional Science and Technology (CREST) Japan Science and Technology Agency K's Gobancho 7 Gobancho Chiyoda-ku Tokyo 102-0076 Japan

We report on helicity-dependent terahertz emissions that originate from the helicity-dependent photocurrents in topological insulator Sb2Te3 thin films due to ultrafast optical excitation. The polarity of the emitted terahertz radiation is controlled by both the incident angle and the helicity of optical pulses. Using an unprecedented decomposition-recombination procedure in the time domain, the signals of the Dirac fermions are fully separated from bulk contributions. These results provide insights into the optical coupling of topological surface states and open up opportunities for applying helicity-dependent terahertz emission spectroscopy in spintronics.

关键词： Optical generation of spin carriers Spin-orbit coupling Surface states Topological materials Topological insulators Ultrafast pump-probe spectroscopy

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Author Correction: The FLUXNET2015 dataset and the ONEFlux processing pipeline for eddy covariance data

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Scientific data 2021年第1期8卷 72页

作者： Gilberto Pastorello Carlo Trotta Eleonora Canfora Housen Chu Danielle Christianson You-Wei Cheah Cristina Poindexter Jiquan Chen Abdelrahman Elbashandy Marty Humphrey Peter Isaac Diego Polidori Markus Reichstein Alessio Ribeca Catharine van Ingen Nicolas Vuichard Leiming Zhang Brian Amiro Christof Ammann M Altaf Arain Jonas Ardö Timothy Arkebauer Stefan K Arndt Nicola Arriga Marc Aubinet Mika Aurela Dennis Baldocchi Alan Barr Eric Beamesderfer Luca Belelli Marchesini Onil Bergeron Jason Beringer Christian Bernhofer Daniel Berveiller Dave Billesbach Thomas Andrew Black Peter D Blanken Gil Bohrer Julia Boike Paul V Bolstad Damien Bonal Jean-Marc Bonnefond David R Bowling Rosvel Bracho Jason Brodeur Christian Brümmer Nina Buchmann Benoit Burban Sean P Burns Pauline Buysse Peter Cale Mauro Cavagna Pierre Cellier Shiping Chen Isaac Chini Torben R Christensen James Cleverly Alessio Collalti Claudia Consalvo Bruce D Cook David Cook Carole Coursolle Edoardo Cremonese Peter S Curtis Ettore D'Andrea Humberto da Rocha Xiaoqin Dai Kenneth J Davis Bruno De Cinti Agnes de Grandcourt Anne De Ligne Raimundo C De Oliveira Nicolas Delpierre Ankur R Desai Carlos Marcelo Di Bella Paul di Tommasi Han Dolman Francisco Domingo Gang Dong Sabina Dore Pierpaolo Duce Eric Dufrêne Allison Dunn Jiří Dušek Derek Eamus Uwe Eichelmann Hatim Abdalla M ElKhidir Werner Eugster Cacilia M Ewenz Brent Ewers Daniela Famulari Silvano Fares Iris Feigenwinter Andrew Feitz Rasmus Fensholt Gianluca Filippa Marc Fischer John Frank Marta Galvagno Mana Gharun Damiano Gianelle Bert Gielen Beniamino Gioli Anatoly Gitelson Ignacio Goded Mathias Goeckede Allen H Goldstein Christopher M Gough Michael L Goulden Alexander Graf Anne Griebel Carsten Gruening Thomas Grünwald Albin Hammerle Shijie Han Xingguo Han Birger Ulf Hansen Chad Hanson Juha Hatakka Yongtao He Markus Hehn Bernard Heinesch Nina Hinko-Najera Lukas Hörtnagl Lindsay Hutley Andreas Ibrom Hiroki Ikawa Marcin Jackowicz-Korczynski Dalibor Janouš Wilma Jans Rachhpal Jassal Shicheng Jiang Tomomichi Kato Myr Computational Research Division Lawrence Berkeley National Laboratory Berkeley CA 94720 USA. gzpastorello@lbl.gov. DIBAF University of Tuscia Viterbo 01100 Italy. Euro-Mediterranean Centre on Climate Change Foundation (CMCC) Lecce 73100 Italy. Climate & Ecosystem Sciences Division Lawrence Berkeley National Laboratory Berkeley CA 94720 USA. Computational Research Division Lawrence Berkeley National Laboratory Berkeley CA 94720 USA. Department of Civil Engineering California State University Sacramento CA 95819 USA. Department of Geography Environment and Spatial Sciences Michigan State University East Lansing MI 48823 USA. Department of Computer Science University of Virginia Charlottesville VA 22904 USA. TERN Ecosystem Processes Menzies Creek VIC3159 Australia. Max Planck Institute for Biogeochemistry Jena 07701 Germany. Laboratoire des Sciences du Climat et de l'Environnement (LSCE) CEA CNRS UVSQ UPSACLAY Gif sur Yvette 91190 France. Key Laboratory of Ecosystem Network Observation and Modeling Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences Beijing 100101 China. Department of Soil Science University of Manitoba Winnipeg MB R3T2N2 Canada. Department of Agroecology and Environment Agroscope Research Institute Zürich 8046 Switzerland. School of Geography and Earth Sciences McMaster University L8S4K1 Hamilton ON Canada. Department of Physical Geography and Ecosystem Science Lund University Lund 22362 Sweden. Department of Agronomy and Horticulture University of Nebraska-Lincoln Lincoln NE 68583 USA. School of Ecosystem and Forest Sciences The University of Melbourne Richmond VIC3121 Australia. Department of Biology Research Group PLECO University of Antwerp Antwerp 2610 Belgium. Joint Research Centre European Commission Ispra 21027 Italy. TERRA Teaching and Research Center University of Liege Gembloux B-5030 Belgium. Finnish Meteorological Institute Helsinki 00560 Finland. ES

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On the Mechanism of BaSi 2 Thin Film Formation on Si Substrate by Vacuum Evaporation

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Procedia Engineering 2016年 141卷 23-26页

作者： Yoshihiko Nakagawa Kosuke O. Hara Takashi Suemasu Noritaka Usami Graduate school of Engineering of Nagoya Nagoya464-8603 Japan Center for Cryatal Science and Technology University of Yamanashi Kofu 400-8511 Japan Core Research for Evolutional Science and Technology Japan Science and Technology Agency Chiyoda Tokyo 102-0076 Institute of Applied Physics University of Tsukuba Tsukuba Ibaraki 305-8573 Japan

We report on the formation mechanism of BaSi 2 thin film on Si substrate grown by vacuum evaporation using BaSi 2 granules as source materials. Since the vapor flux at the initial stage of evaporation is known to be Ba-rich, Si supply from the substrate is of crucial importance to obtain homogeneous BaSi 2 thin film. In fact, low substrate temperature and/or thick film deposition led to formation of rough film with voids, and the oxidation proceeded upon exposure to air. We revealed that appropriate choice of substrate temperature, film thickness, and post-growth in-situ annealing can provide enough diffusion of Si and Ba, leading to realization of homogeneous BaSi 2 thin film.

关键词： barium desiliside vacuum evaporation

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Development of a core-shell nanostructured photoanode for efficient water splitting for a light driven μ-fuel generation device 19

Development of a core-shell nanostructured photoanode for ef...

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19th International Conference on Miniaturized Systems for Chemistry and Life sciences, MicroTAS 2015

作者： Pihosh, Y. Uemura, J. Mawatari, K. Kitamori, T. University of Tokyo Department of Applied Chemistry School of Engineering Japan Japan Science and Technology Agency Core Research for Evolutional Science and Technology Japan

ISBN: (纸本)9780979806483

In this work we report on the development and realization of a nanostructured heterojunction photoanode that is based on an extremely thin absorber layer for effective separation and transfer of photo-induced charge carriers to enhance the water splitting process. We demonstrate this concept on a WO3/BiVO4+CoPi core-shell nanostructure, which was realized with the combination of Glancing Angle Deposition (GLAD) and Electrochemical Deposition (ED) techniques, and report a record of a generated photocurrent that is close to 90% of the theoretical maximum value with further possible practical application in a μ-fuel generation device. © 15CBMS-0001.

关键词： Heterojunctions

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Development and Realization of a Solar Light Driven μ-Fuel Cell Device on a Microfluidic Chip Platform 19

Development and Realization of a Solar Light Driven μ-Fuel ...

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19th International Conference on Miniaturized Systems for Chemistry and Life sciences, MicroTAS 2015

ISBN: (纸本)9780979806483

In this work, we report on the development and realization of a totally integrated solar light driven μ-fuel cell (μ-FC) device integrated on a microfluidic chip platform. We developed a new type of a core-shell nanostructured heterojunction WO3/BiVO4 photoanode for efficient fuel production under solar light illumination and successfully integrated it into the light driven μ-FC device with the help of top down and bottom up technological processes. We describe the device concept, explain its main functional units which are based on the specific properties of micro/nano fluidics and finally demonstrate its working principle under solar light illumination. © 15CBMS-0001.

关键词： Microfluidics

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Simple Vacuum Evaporation Route to BaSi 2 Thin Films for Solar Cell Applications

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Procedia Engineering 2016年 141卷 27-31页

作者： Kosuke O. Hara Yoshihiko Nakagawa Takashi Suemasu Noritaka Usami Center for Crystal Science and Technology University of Yamanashi 7-32 Miyamae Kofu Yamanashi 400-8511 Japan Core Research for Evolutional Science and Technology Japan Science and Technology Agency Gobancho Chiyoda Tokyo 102-0075 Japan Graduate School of Engineering Nagoya University Furo-cho Chikusa-ku Nagoya 464-8603 Japan Institute of Applied Physics University of Tsukuba 1-1-1 Tennohdai Tsukuba Ibaraki 305-8573 Japan

The BaSi 2 semiconductor has excellent optoelectronic properties for solar cell applications and consists of earth-abundant elements. In this study, we have investigated the thin film growth of BaSi 2 by a simple vacuum evaporation technique. It is shown by X-ray diffraction that single-phase BaSi 2 films can be formed at high substrate temperatures of 500 and 600 ˚C on either Si(111) or EAGLE XG glass substrate. The film growth mechanism is discussed on the basis of the transmission electron microscopy observation and energy-dispersive X-ray spectra of the film and the residue of evaporation source. It is concluded that high substrate temperatures are needed to remove excess Ba atoms and to eliminate a composition gradient. In addition, suitable optical properties for solar cell applications are revealed by analyzing the transmittance and reflectance spectra.

关键词： Silicide semiconductor vacuum evaporation crystal growth optical properties

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