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arXiv 2023年

作者： Guo, Tianzheng Zou, Xiaoyu Sundar, Shalini Jia, Xinqiao Dhong, Charles Department of Materials Science and Engineering University of Delaware NewarkDE United States Department of Biomedical Engineering University of Delaware NewarkDE United States

Recent advances recognize that the viscoelastic properties of epithelial structures play important roles in biology and disease modeling. However, accessing the viscoelastic properties of multicellular structures in mechanistic or drug-screening applications face challenges in repeatability, accuracy, and practical implementation. Here, we present a microfluidic platform that leverages elastohydrodynamic phenomena, sensed by graphene strain sensors, to measure the viscoelasticity of cellular monolayer in situ, without using labels or specialized equipment. We demonstrate platform utility with two systems: cell dissociation following trypsinization, where viscoelastic properties change over minutes, and an epithelial-to-mesenchymal transition, where changes occur over days. These cellular events could only be resolved with our platform’s higher signal-to-noise ratio: relaxation times of 14.5 ± 0.4 s-1 for intact epithelial monolayers versus 13.4 ± 15.0 s-1 in other platforms. By rapidly assessing combined contributions from cell stiffness and intercellular interactions, we anticipate that the platform will hasten translation of new mechanical biomarkers. © 2023, CC BY-NC-ND.

关键词： Monolayers

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Fluorescent carbon dots for labeling of bacteria: mechanism and prospects—a review

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Analytical and Bioanalytical Chemistry 2024年第17期416卷 3907-3921页

作者： Anand, Anisha Huang, Chih-Ching Lai, Jui-Yang Bano, Darakhshan Pardede, Helen Indah Hussain, Amina Saleem, Sehresh Unnikrishnan, Binesh Department of Biomedical Engineering Chang Gung University Taoyuan33302 Taiwan Department of Bioscience and Biotechnology National Taiwan Ocean University Keelung202301 Taiwan Center of Excellence for the Oceans National Taiwan Ocean University Keelung202301 Taiwan School of Pharmacy College of Pharmacy Kaohsiung Medical University Kaohsiung80708 Taiwan Department of Ophthalmology Chang Gung Memorial Hospital Taoyuan Linkou33305 Taiwan Department of Materials Engineering Ming Chi University of Technology New Taipei City24301 Taiwan Research Center for Chinese Herbal Medicine College of Human Ecology Chang Gung University of Science and Technology Taoyuan33303 Taiwan

The search for bacteria-labeling agents that are more efficient and less toxic compared to existing staining dyes is ongoing. Fluorescent quantum dots and carbon dots (CDs) have been extensively researched for various bioimaging applications. Priority is given to CDs due to several advantages, including lower toxicity, versatility in tuning their properties, and better photostability compared to metal-based quantum dots. Although significant progress is still needed to replace existing dyes with CDs for bacteria labeling, they offer promising potential for further improvement in efficiency. Surface charges and functional groups have been reported as decisive factors for bacterial discrimination and live/dead assays;however, a complete guideline for preparing CDs with optimum properties for efficient staining and predicting their labeling performance is lacking. In this review, we discuss the application of fluorescent CDs for bacterial labeling and the underlying mechanisms and principles. We primarily focus on the application and mechanism of CDs for Gram differentiation, live imaging, live/dead bacteria differentiation, bacterial viability testing, biofilm imaging, and the challenges associated with application of CDs. Based on proposed mechanisms of bacterial labeling and ambiguous results reported, we provide our view and guidelines for the researchers in this field to overcome the challenges associated with bacteria labeling using fluorescent CDs. Graphical Abstract: (Figure presented.) © The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature 2024.

关键词： Bacteria

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Modifying BiVO4 as a photocatalyst for water oxidation using constant-duration alkaline-etched post treatments

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New Journal of Chemistry 2023年第2期48卷 569-577页

作者： Hsu, Chuan-Chih Chuang, Kai-Jie Huang, Hsiao-Wen Pan, Bo-Jin Chen, Hung-Ming Husain, Sadang Yougbaré, Sibidou Hsiao, Yu-Cheng Wu, Yung-Fu Lin, Lu-Yin Division of Cardiovascular Surgery Department of Surgery School of Medicine College of Medicine Taipei Medical University Taiwan Division of Cardiovascular Surgery Department of Surgery Taipei Medical University Hospital Taiwan Department of Chemical Engineering and Biotechnology National Taipei University of Technology Taipei Taiwan Department of Chemical Engineering Ming Chi University of Technology New Taipei City24301 Taiwan Gingen Technology Co. Ltd. Rm. 7 10F. No. 189 Sec. 2 Keelung Rd. Xinyi Dist. Taipei11054 Taiwan Department of Physics Faculty of Mathematics and Natural Science Lambung Mangkurat University Banjarmasin70124 Indonesia /Nanoro Ouagadougou03 Burkina Faso International PhD Program in Biomedical Engineering College of Biomedical Engineering Taipei Medical University Taipei11031 Taiwan Stanford Byers Center for Biodesign Stanford University CA94305-5428 United States Graduate Institute of Biomedical Optomechatronics College of Biomedical Engineering Taipei Medical University Taipei11031 Taiwan Cell Physiology and Molecular Image Research Center Wan Fang Hospital Taipei Medical University Taipei11696 Taiwan

BiVO4 with suitable band edges and a small band gap is considered an efficient photocatalyst for the oxygen evolution reaction (OER), but short charge-transfer lengths limit its photocatalytic ability. Increasing active sites can essentially enhance oxidation reactions and photocatalytic ability. The alkaline etching technique can refine the surface properties of BiVO4 by introducing oxygen vacancies and possibly establishing a heterojunction, but suitable alkaline etching methods are rarely discussed. In this study, three alkaline etching methods, including hydrothermal, soaking, and electrodeposition processes, are first applied for a constant duration to tailor the surface properties of BiVO4 as a photocatalyst for the OER. Alkaline-etched BiVO4 prepared using hydrothermal and soaking processes shows better photocatalytic abilities due to the generation of additional oxygen vacancies, while poor photocatalytic ability is found for that fabricated using electrodeposition due to the formation of less photocatalytic VO2. The highest photocurrent density of 2.38 mA cm−2 is obtained at 1.23 V versus a reversible hydrogen electrode in an electrolyte without a hole scavenger for the BiVO4 etched in the hydrothermal process, while the untreated BiVO4 presents a photocurrent density of 0.58 mA cm−2. A photocurrent retention of 92.3% is achieved after 5000 seconds of continuous illumination. The study supplies blueprints for establishing effective alkaline etching methods for enhancing the photocatalytic ability of BiVO4, with the exception of time costs. © 2024 The Royal Society of Chemistry.

关键词： Charge transfer

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The synergistic effects of strontium/magnesium-doped calcium silicate cement accelerates early angiogenesis and bone regeneration through double bioactive ion stimulation

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Ceramics International 2024年第4期50卷 7121-7131页

作者： Wang, Chen-Ying Chen, Cheng-Yu Chen, Kun-Hao Lin, Yen-Hong Yeh, Tzu-Peng Kai-Xing Lee, Alvin Huang, Cheng-Chieh Shie, Ming-You Graduate Institute of Clinical Dentistry School of Dentistry National Taiwan University Taipei106319 Taiwan Division of Periodontics Department of Dentistry National Taiwan University Hospital Taipei106319 Taiwan Research & Development Center for x-Dimensional Extracellular Vesicles China Medical University Hospital Taichung404332 Taiwan School of Medicine China Medical University Taichung City406040 Taiwan Department of Dental Technology and Materials Science Central Taiwan University of Science and Technology Taichung City406053 Taiwan Department of Orthopedic Surgery China Medical University Hospital Taichung City404332 Taiwan Department of Biomedical Engineering China Medical University Taichung406040 Taiwan Department of Bioinformatics and Medical Engineering Asia University Taichung City41354 Taiwan

Periodontitis-induced alveolar bone defects necessitate the use of bone grafts for optimal therapeutic outcomes. Therefore, synthetic bone grafts exhibiting consistent properties, sufficient amounts, and multiple application forms have been extensively studied. Our previously designed Ca–Si–Mg cement has demonstrated benefits regarding cell proliferation, osteogenesis, and angiogenesis. Similarly, Sr exhibits osteogenic, anti-osteoclastogenic, and angiogenic properties by influencing several intracellular signaling pathways. In this study, we modified the previously established Ca–Si–Mg cement by incorporating Sr, which resulted in the formation of Mg–Sr-calcium silicate (CS) cement. Furthermore, we evaluated its biocompatibility and efficacy in promoting osteogenesis and angiogenesis. The results revealed that the setting time of the cement was extended with the individual incorporation of either Mg or Sr;notably, the setting time of M5S5 (integrating both Sr and Mg in equal proportions) was 2.7 min shorter than that of M5S0 (integrating only Mg). In addition, the presence of Sr enhanced the mechanical strength to an ultimate strength of 1.89 MPa and reduced the biodegradation rate of the Mg-doped CS cement from a weight loss of 13.9 %–11.5 %. Sr and Mg also demonstrated a synergistic effect on cell proliferation, with no observed cytotoxicity in human periodontal ligament cells (hPDLs). Osteogenic markers such as alkaline phosphate, bone sialoprotein, and osteocalcin were significantly expressed in hPDLs under the combined influence of Mg and Sr on osteogenic activity. Similarly, the angiogenesis-related proteins, vascular endothelial growth factor and angiopoietin-1 exhibited considerable enhancement, indicating the angiogenic capability of M5S5. In summary, our in vitro experiments confirmed that M5S5 is a potential candidate for osteogenic substitutes and alveolar bone regeneration, suggesting its potential applicability in clinical practice. © 2023 Elsevier Lt

关键词： Cements

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Decoding White Matter Fiber ODFs: A Mixture Learning Framework in x-q Space

Decoding White Matter Fiber ODFs: A Mixture Learning Framewo...

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2024 IEEE International Conference on Bioinformatics and Biomedicine, BIBM 2024

作者： Ma, Jiquan Deng, Chengdong Chen, Geng Jiang, Haotian Huang, Shijie Kim, Jaeil Wen, Xuyun Shen, Dinggang Heilongjiang University Department of Computer Science and Technology Harbin China Northwestern Polytechnical University School of Computer Science and Engineering Xi'an China Kyungpook National University School of Computer Science and Engineering Daegu Korea Republic of Nanjing University of Aeronautics and Astronautics School of Computer Science and Technology Nanjing China ShanghaiTech University School of Biomedical Engineering State Key Laboratory of Advanced Medical Materials and Devices Shanghai China Shanghai United Imaging Intelligence Co. Ltd. Shanghai China Shanghai Clinical Research and Trial Center Shanghai China

ISBN: (纸本)9798350386226

Diffusion magnetic resonance imaging (dMRI), as a powerful non-invasive white matter imaging technology, plays an important role in studying brain white matter. The fiber orientation distribution functions (fODFs) derived from dMRI data provide the key directional information of fiber tracts for revealing the 3D geometric structure of brain white matter. The estimation of fODFs faces two challenges, including (i) the demand for dMRI data densely sampled in q-space and (ii) the joint consideration of x-q space. To address these challenges, we propose a mixture learning framework with q-space sparely sampled dMRI data as input. Specifically, we propose an x-space learning module based on 3D U-Net to learn x-space features and a q-space learning module based on spherical convolutional neural networks to learn q-space features. Two kinds of features are then fused with a mixture learning fusion module for fODFs estimation. The whole framework is supervised with an x-q space loss function. Our framework makes full use of joint x-q space information for fODFs estimation with clinically available q-space sparsely sampled dMRI data. Extensive experiments on three public datasets show that our framework is effective in fODFs estimation and outperforms cutting-edge models. © 2024 IEEE.

关键词： Magnetic resonance imaging

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Microstructural Control of Zn Alloy by Melt Spinning - a Novel Approach Towards Fabrication of Advanced Biodegradable biomedical materials

SSRN

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SSRN 2025年

作者： Rahim, Shebeer Abdul Tomei, Joseph Licavoli, Joseph Bakhsheshi-Rad, Hamid R. Goldman, Jeremy Drelich, Jaroslaw W. Department of Materials Science and Engineering Michigan Technological University 1400 Townsend Drive HoughtonMI49931 United States Advanced Materials Research Center Department of Materials Engineering Islamic Azad University Najafabad Branch Najafabad Iran Department of Biomedical Engineering Michigan Technological University 1400 Townsend Drive HoughtonMI49931 United States

Biodegradable metallic stents that dissolve over time are essential for treating vascular artery disease. Previous designs made from polymers and magnesium have not achieved the required mechanical properties and degradation patterns. Here, we report a novel zinc alloy that possesses a combination of high strength, good ductility, and uniform degradation behavior. The Zn-0.9Cu-0.4Mn-0.01Mg alloy is produced using melt spinning (a rapid solidification technique), compaction, and extrusion to enhance the synergy between strength and ductility. The melt-spun extruded alloy exhibits an elongation to failure of nearly 30% and a tensile strength exceeding 320 MPa, meeting the mechanical performance criteria required for vascular stenting materials. Melt spinning results in weak texture facilitating basal slip dislocations, and promoting ductility, while maintaining high strength. The microstructure of the melt-spun alloy displays a more uniform and finer microstructure as compared to the extruded alloy. The fine grain size and the uniform dispersion of secondary phases contribute to the uniform degradation behavior of the melt-spun extruded alloy, with a corrosion rate of ~ 0.6 mm/year and low corrosion current density of ~ 40 µA/cm2. The findings suggest that rapid solidification of zinc alloys through melt spinning is a promising approach for developing biodegradable medical implants of predictable degradation. © 2025, The Authors. All rights reserved.

关键词： Ductility

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Nano-Heterostructured materials - Based Sensors for Safety and biomedical Applications 12

Nano-Heterostructured Materials - Based Sensors for Safety a...

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12th IEEE International Conference "Nanomaterials: Applications and Properties", NAP 2022

作者： Lupan, Oleg Magariu, Nicolae Kruger, Helge Sereacov, Alexandr Ababii, Nicolai Railean, Serghei Zimoch, Lukas Adelung, Rainer Hansen, Sandra Technical University Of Moldova Center For Nanotechnology And Nanosensors Department Of Microelectronics And Biomedical Engineering Chisinau Moldova Christian-Albrechts Universität Zu Kiel Faculty Of Engineering Department Of Materials Science Chair For Functional Nanomaterials KielD-24143 Germany

ISBN: (数字)9781665489829

ISBN: (纸本)9781665489829

Permanent monitoring of lithium-ion batteries (LIBs) and volatile organic compounds (VOCs) in various environments, especially for safety and biomedical applications, is a growing field due to the high reactivity of the materials used, which require specialized sensor structures. In this work, we summarize the detection performance of metal oxide heterostructures against battery solvents and volatile organic compounds and propose a way to tailor the sensor selectivity by modifying structural properties on the nanoscale. Therefore, the oxides are grown by a simple chemical solution method and by thermal layer deposition followed by thermal annealing at various temperatures. Subsequently, the morphology and structure as well as the electronic, chemical, and sensing properties of the formed semiconducting oxide heterostructures are investigated. Gas sensing studies have shown that the surface coverage with metal oxides and the formation of nano-heterostructures is an efficient approach to improve the LIB electrolyte sensing. The present approach demonstrates that the combination of the ability to sense the electrolyte vapors used in LIBs and the size control of different oxides enabled by the used synthesis route makes these nano-heterostructures extremely attractive for all kinds of sensing purposes, especially for battery safety control and biomedical applications. Our developments are very important for future LIB sensors and necessary for understanding the effect of the heterostructure type and the thickness of the top nanofilm on the gas response, which thus far has not been reported in the literature. © 2022 IEEE.

关键词： Zinc oxide

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Piezoelectric electrostatic superlattices in monolayer MoS2

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Physical Review materials 2024年第1期8卷 014002-014002页

作者： Ashwin Ramasubramaniam Doron Naveh Department of Mechanical and Industrial Engineering University of Massachusetts Amherst 01003 MA United States Materials Science and Engineering Graduate Program University of Massachusetts Amherst 01003 MA United States Faculty of Engineering Bar-Ilan University Ramat Gan 52900 Israel Institute for Nanotechnology and Advanced Materials Bar-Ilan University Ramat Gan 52900 Israel

Modulation of electronic properties of materials by electric fields is central to the operation of modern semiconductor devices, providing access to complex electronic behaviors and greater freedom in tuning the energy bands of materials. Here, we explore one-dimensional superlattices induced by a confining electrostatic potential in monolayer MoS2, a prototypical two-dimensional semiconductor. Using first-principles calculations, we show that periodic potentials applied to monolayer MoS2 induce electrostatic superlattices in which the response is dominated by structural distortions relative to purely electronic effects. These structural distortions reduce the intrinsic band gap of the monolayer substantially while also polarizing the monolayer through piezoelectric coupling, resulting in spatial separation of charge carriers as well as Stark shifts that produce dispersive minibands. Importantly, these minibands inherit the valley-selective magnetic properties of monolayer MoS2, enabling fine control over spin-valley coupling in MoS2 and similar transition-metal dichalcogenides.

关键词： Electronic structure Piezoelectricity Valley degrees of freedom

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PtS-decorated WS2 microflakes based sensors for selective ammonia detection at room temperature

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Sensors and Actuators B: Chemical 2023年第1期394卷

作者： Guang, Qiyilan Huang, Baoyu Yu, Jun Bonyani, Maryam Moaddeli, Mohammad Kanani, Mansour Mirzaei, Ali Kim, Hyoun Woo Kim, Sang Sub Li, Xiaogan School of Microelectronics Dalian University of Technology Dalian116024 China School of Biomedical Engineering Dalian University of Technology Dalian116024 China Department of Materials Science and Engineering School of Engineering Shiraz University Shiraz Iran Department of Materials Science and Engineering Shiraz University of Technology Shiraz Iran Division of Materials Science and Engineering Hanyang University Seoul133-791 Korea Republic of The Research Institute of Industrial Science Hanyang University Seoul133-791 Korea Republic of Department of Materials Science and Engineering Inha University Incheon402-751 Korea Republic of

In this paper, platinum sulfide decorated WS2 microflakes (PtS/WS2) heterostructure nanocomposites were prepared by an in-situ chemical reduction process. The chemiresistive-type gas sensor based on the synthesized PtS/WS2 heterostructure nanocomposites exhibited a significantly improved sensing properties compared to that of the pure WS2 microflakes at different concentration of NH3 from 1 ppm to 10 ppm at room temperature. As the concentrations of PtS nanoparticles decorated on the surface of WS2 microflakes increased, the response of the PtS/WS2 heterostructure sensor to ammonia firstly increased when the concentration of PtS increased up to 1 wt%, whereas above 1%, the response decreased instead. Moreover, it was found that the response speeds of the PtS-decorated WS2 were significantly improved compared to that pure WS2. The sensors also exhibited excellent selectivity to formaldehyde, toluene, acetone, methanol and ethanol at room temperature. The sensing mechanisms were studied by using the in-situ diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS). The enhanced sensing properties could be explained by "electronic sensitization" effect induced by the PtS/WS2 heterostructure, being confirmed by density functional theory (DFT) calculations. © 2023

关键词： Fourier transform infrared spectroscopy

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Diffractive Imaging of Transient Electronic Coherences in Molecules with Electron Vortices

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Physical Review Letters 2025年第7期134卷 073001-073001页

作者： Haowei Wu Haiwang Yong Department of Chemistry and Biochemistry University of California San Diego La Jolla California 92093 USA Program in Materials Science and Engineering University of California San Diego La Jolla California 92093 USA

Direct imaging of transient electronic coherences in molecules has been challenging, with the potential to control electron motions and influence reaction outcomes. We propose a novel time-resolved vortex electron diffraction technique to spatially resolve transient electronic coherences in isolated molecules. By analyzing helical dichroism diffraction signals, the contribution of electronic populations cancels out, isolating the purely electronic coherence signals. This allows direct monitoring of the time evolution and decoherence of transient electronic coherences in molecules.

关键词： Molecules

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