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Beneath the waves: Unveiling the potent cytotoxic power of S. marginatum

Oral Oncology Reports 2024年 10卷 100349-100349页

作者： Arul, Balasubramanian Saravanan, Muniyappan Silambarasan, Tamil Selvan Venkatesan, Perumal Kothai, Ramalingam Department of Pharmacy Practice Vinayaka Missions College of Pharmacy Vinayaka Missions Research Foundation (Deemed to be University) Salem 636008 Tamil Nadu India Centre for Global Health Research Saveetha Medical College and Hospitals Saveetha Institute of Medical and Technical Sciences (SIMATS) Saveetha University Chennai 602105 Tamil Nadu India Center for Injury Biomechanics Materials and Medicine Department of Biomedical Engineering New Jersey Institute of Technology Newark 07102 USA

Highlights•Seaweed is recognized for its rich source of novel and promising lead biomolecules, particularly in brown seaweeds. •Seaweed polysaccharides exhibit diverse biological activities, including anticancer, antioxidant, antiviral, and etc. •Seaweed polysaccharides like alginic acid, fucoidans, and laminarians are widely used in pharmaceutical formulations. •Ethanolic extracts of S. marginatum show cytotoxic activity against various cancer cell lines, including choriocarcinoma. •S. marginatum has potential for developing therapies to treat OSCC through its biomedical secondary metabolites.

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A simple and label-free dual-modal aptazyme for fluorescent/colorimetric sensitive on-site detection of small molecules in real samples

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Sensors and Actuators B: Chemical 2025年 442卷

作者： Luo, Yingping Wu, Guohua Lu, Qinrui Lai, Tongtong Xie, Linhui Zhang, Jingyan Niu, Shiwen Tang, Jiancai Yu, Haixiang Department of Pharmacology North Sichuan Medical College Nanchong637100 China Henan Key Laboratory of Rare Diseases Endocrinology and Metabolism Center The First Affiliated Hospital and College of Clinical Medicine of Henan University of Science and Technology Luoyang471003 China Department of Biomedical Engineering Sichuan University Chengdu610065 China School of Basic Medical Sciences Tianjin Medical University Tianjin300070 China Department of Basic Medical Sciences North Sichuan Medical College Nanchong637100 China School of Life Science and Technology China Pharmaceutical University Nanjing211198 China

Dual-modal biosensors integrating colorimetric and fluorescent signal reporters have demonstrated high robustness and accuracy for onsite small-molecule detection. However, the development of such assays is limited by the lack of a universal reporter for both detection modes. In this study, we leverage the activity of G-quadruplex DNAzyme to catalyze both fluorescent and chromogenic substrates, presenting a generalizable strategy to engineer label-free dual-mode aptazyme (LDAzyme), for the naked-eye detection of small molecules. An LDAzyme consists of two strands that self-assemble to form an active G-quadruplex DNAzyme in the absence of the target. Upon target binding, a conformational change dissociates the LDAzyme and inactivates the DNAzyme motif. This target-induced change in aptazyme activity enables the reporting of target concentrations fluorescently and colorimetrically using fluorescent (rhodamine B) and chromogenic (2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid, ABTS) substrates, respectively. As a demonstration, we develope aflatoxin B1- and lactate-activated LDAzymes, which achieved sensitive and specific detection of their targets in both fluorescence and colorimetry modes. The similar dynamic ranges of the two modes facilitate the robust quantitation of the analyte in complex matrices, including herbal and food extracts and serum. Notably, the high activity of LDAzymes allows for the visual detection of targets in real samples within 15 min in both modes. Given the favorable sensing characteristics of LDAzymes, we believe that such a design strategy could be widely applied to various analytes by substituting the corresponding aptamer sequence in the construct, which should accelerate the development of on-site or mix-and-measure assays. © 2025 Elsevier B.V.

关键词： Colorimetry

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Multimodal spatial gradients to explain regional susceptibility to fibrillar tau in Alzheimer's disease

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Alzheimer's and Dementia 2025年第5期21卷 e70170页

作者： Luan, Ying Zheng, Lukai Denecke, Jannis Dehsarvi, Amir Roemer-Cassiano, Sebastian N. Dewenter, Anna Steward, Anna Shcherbinin, Sergey Svaldi, Diana Otero Kotari, Vikas Higgins, Ixavier Alonzo Pontecorvo, Michael J. Valentim, Carolina Schnabel, Julia A. Casale, Francesco Paolo Dyrba, Martin Teipel, Stefan Franzmeier, Nicolai Ewers, Michael Department of Radiology Zhongda Hospital School of Medicine Southeast University Nanjing China Institute for Stroke and Dementia Research (ISD) University Hospital Ludwig Maximilian University (LMU) Munich Germany Eli Lilly and Company Indianapolis IN United States Institute of Machine Learning in Biomedical Imaging Helmholtz Munich Neuherberg Germany TUM School of Computation Information and Technology & TUM Institute for Advanced Study Technical University of Munich Munich Germany School of Biomedical Engineering and Imaging Sciences King's College London London Strand United Kingdom German Center for Neurodegenerative Diseases (DZNE) Rostock Germany Department of Psychosomatic Medicine Rostock University Medical Center Rostock Germany Munich Cluster for Systems Neurology (SyNergy) Munich Germany Department of Psychiatry and Neurochemistry The Sahlgrenska Academy Institute of Neuroscience and Physiology University of Gothenburg Gothenburg Sweden

INTRODUCTION: In Alzheimer's disease (AD), fibrillar tau gradually progresses from initial seed to larger brain area. However, those brain properties underlying the region-dependent susceptibility to tau accumulation remain unclear. METHODS: We constructed multimodal spatial gradients to characterize molecular properties and connectomic architecture. A predictive model for regional tau deposition was developed by integrating embeddings in the principal gradients of global connectome gradients with gene expression, neurotransmitters, myelin, and amyloid-beta. The model was trained on amyloid-beta–positive participants from Alzheimer's Disease Neuroimaging Initiative (ADNI) and externally validated in independent datasets. RESULTS: The combination of gradients explained up to 77.7% of cross-sectional and 77.3% of longitudinal inter-regional variance of tau deposition. Gene set enrichment analysis of a major gene expression gradient points to synaptic transmission to confer increased susceptibility to tau. DISCUSSION: Our findings reveal a spatially heterogeneous molecular landscape shaping regional susceptibility to tau deposition, presenting a powerful system-level explanatory model of tau pathology in AD. Highlights: Spatial gradients of fundamental molecular brain properties associated with tau pathology. The explanatory power showed high consistency across studies. Genetic analyses suggested that synapse expression plays a vital role in tau accumulation. © 2025 The Author(s). Alzheimer's & Dementia published by Wiley Periodicals LLC on behalf of Alzheimer's Association.

关键词： Alzheimer's disease functional connectivity gene expression multimodal gradients neurotransmitters predictive model tau positron emission tomography

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Improvement of extracellular polysaccharides production from Cordyceps militaris immobilized alginate beads in repeated-batch fermentation

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LWT 2024年 193卷

作者： Hou, Shi-Jing Cheng, Kuan-Chen Lin, Shyh-Hsiang Hsiao, I-Lun Santoso, Shella Permatasari Singajaya, Stephanie Chou, Yu-Chieh Lin, Shin-Ping Master Program in Food Safety Taipei Medical University 250 Wu-Hsing Street Taipei11031 Taiwan Institute of Biotechnology National Taiwan University 1 Roosevelt Rd. Sec. 4 Taipei10617 Taiwan Graduate Institute of Food Science and Technology National Taiwan University 1 Roosevelt Rd. Sec. 4 Taipei10617 Taiwan Department of Medical Research China Medical University Hospital China Medical University 91 Hsueh-Shih Rd. Taichung40402 Taiwan Department of Optometry Asia University 500 Lioufeng Rd. Wufeng Taichung41354 Taiwan School of Nutrition and Health Sciences Taipei Medical University 250 Wu-Hsing Street Taipei11031 Taiwan School of Food Safety Taipei Medical University 250 Wu-Hsing Street Taipei11031 Taiwan Department of Chemical Engineering Widya Mandala Surabaya Catholic University Kalijudan 37 East Java Surabaya60114 Indonesia Collaborative Research Center for Sustainable and Zero Waste Industries Kalijudan 37 East Java Surabaya60114 Indonesia Ph.D. Program in Drug Discovery and Development Industry Taipei Medical University 250 Wu-Hsing Street Taipei11031 Taiwan Research Center of Biomedical Device Taipei Medical University 250 Wu-Hsing Street Taipei11031 Taiwan TMU Research Center for Digestive Medicine Taipei Medical University 250 Wu-Hsing Street Taipei11031 Taiwan

Cordyceps militaris (C. militaris) is a unique medicinal fungus of the genus Cordyceps. It has high economic value due to various biological functions from the extracellular polysaccharides (EPSs) it produces. The aim of this study was to establish a mycelium-immobilization system for EPS production in a bubble column system. First, EPS production was optimized by 90.2% (from 0.72 to 1.37 g/L) using response surface methodology under an alginate-immobilized mycelium system with 6 days of cultivation. The highest EPS production was obtained (5.3 g/L) in a flask system through elongation to 15 days of cultivation. EPS production subsequently further increased to 10.42 g/L utilizing a bubble column reactor. Furthermore, the system can be reused for at least three rounds. In the EPS characteristics analysis, the produced EPSs were mainly composed of glucose and galactose. They presented strong antibacterial activity against Staphylococcus aureus. In terms of antioxidant activity, respective the EPS concentration that caused 50% scavenging of DPPH and ABTS free radicals values (IC50) were 4.65 and 7.92 mg/mL. In summary, these findings should be helpful for establishing a suitable industrial fermentation system for C. militaris and can be studied in the food and medical industries in future work. © 2024 The Authors

关键词： Mycelium

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The microstructural evolution and the processing map of extruded Mg-4Al-1Si magnesium alloys

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Journal of Alloys and Compounds 2025年 1035卷

作者： Xiaolong Mi Lifei Wang Xiaoye Zhou Jun Cheng Xiao Wang Kunkun Deng Zhiyong You Kwang Seon Shin Shanxi Key Laboratory of Magnesium-based Materials College of Materials Science and Engineering Taiyuan University of Technology Taiyuan 030024 China Department of Materials Science and Engineering Shenzhen MSU-BIT University Shenzhen 518172 China Northwest Institute for Non-ferrous Metal Research Shaanxi Key Laboratory of Biomedical Metal Materials Xi’an 710016 China National Key Laboratory of Helicopter Aeromechanics Nanjing University of Aeronautics and Astronautics Nanjing 210016 China Magnesium Technology Innovation Center School of Materials Science and Engineering Seoul National University Gwanak-ro Gwanak-gu Seoul 08826 South Korea

Owing to its remarkable creep resistance and cost-efficient production, the Mg-4Al-1Si magnesium alloy presents considerable potential for widespread industrial utilization. Nevertheless, the hot deformation behavior and key processing characteristics of this alloy have not yet been fully elucidated, requiring further investigation. Therefore, this study establishes a hot processing map for the extruded Mg-4Al-1Si alloy and identifies its optimal processing window. We systematically investigate the microstructural evolution and dynamic recrystallization (DRX) mechanisms in both stable and unstable regions of the processing map. Moreover, the dominant deformation mechanisms in the stable regions are investigated through combined in-grain misorientation axis analysis and viscoplastic self-consistent modeling. The experimental results demonstrate that the extruded Mg-4Al-1Si alloy exhibits an optimal processing window within the temperature range of 640–723 K and strain rate range of 0.0001–0.0015 s⁻¹ at a true strain of 0.2. Microstructural analysis reveals that discontinuous DRX (DDRX) dominates in unstable processing regions, while stable regions exhibit a synergistic effect of continuous DRX (CDRX) and DDRX mechanisms. As the strain increasing, CDRX becomes significantly enhanced in the stable region. Furthermore, basal and pyramidal slip systems constitute the primary deformation mechanisms operating in the stable region. This study provides critical guidance for the design of hot deformation processes in Mg-4Al-1Si Mg alloys.

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The role of bone marrow-derived cells in the origin of liver cancer revealed by single-cell sequencing

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Cancer Biology & Medicine 2020年第1期17卷 142-153页

作者： Lu Chen Xianfu Yi Piao Guo Hua Guo Ziye Chen Chunyu Hou Lisha Qi Yongrong Wang Chengwen Li Peng Liu Yucun Liu Yuanfu Xu Ning Zhang The concrete information of affiliations should be presented for instanceDepartment of Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjin's Clinical Research Center for CancerTianjin 300070China School of Biomedical Engineering and Technology Tianjin Medical UniversityTianjin 300070China The Center for Translational Cancer Research Peking University First HospitalBeijing 100034China State Key Laboratory of Experimental Hematology National Clinical Research Center for Blood DiseasesInstitute of Hematology&Blood Diseases HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeTianjin 300020China CAMS Key Laboratory for Prevention and Control of Hematological Disease Treatment Related Infection Tianjin 300020China Cytogenetics Laboratory Institute of Hematology and Blood Diseases HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeTianjin 300020China

Objective:Epithelial cancers often originate from progenitor cells,while the origin of hepatocellular carcinoma(HCC)is still ***,one of the deadliest cancers,is closely linked with liver injuries and chronic inflammation,which trigger massive infiltration of bone marrow-derived cells(BMDCs)during liver ***:To address the possible roles of BMDCs in HCC origination,we established a diethylnitrosamine(DEN)-induced HCC model in bone marrow transplanted *** and frozen tissue immunofluorescence were used to verify DENinduced HCC in the pathology of the *** cellular origin of DEN-induced HCC was further studied by single cell sequencing,single-cell nested PCR,and immunofluorescence-fluorescence in situ ***:Studies by using single cell sequencing and biochemical analysis revealed that HCC cells in these mice were coming from donor mice BMDCs,and not from recipient ***,the copy numbers of mouse orthologs of several HCC-related genes previously reported in human HCC were also altered in our mouse ***-induced HCCs exhibited a similar histological phenotype and genomic profile as human ***:These results suggested that BMDCs are an important origin of HCC,which provide important clues to HCC prevention,detection,and treatments.

关键词： Hepatocellular carcinoma bone marrow-derived cells(BMDCs) origination genome sequencing copy number alteration

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Internal force-controlled (IFC) sonication with tunable conditions for the large-scale preparation of porous silicon nanoparticles

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Journal of Drug Delivery Science and technology 2025年 110卷

作者： Hwang, Nam Cheol Baek, Seung Woo Choi, Dongjae Jeong, Hwajun Kim, Dokyoung Department of Biomedical Science Kyung Hee University Seoul 02447 South Korea College of Medicine Kyung Hee University Seoul 02447 South Korea College of Medicine Yonsei University Seoul 03722 South Korea Department of Anatomy College of Medicine Chung-Ang University Seoul 06974 South Korea Department of Anatomy and Neurobiology College of Medicine Kyung Hee University Seoul 02447 South Korea Department of Precision Medicine Kyung Hee University Seoul 02447 South Korea Medical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute School of Medicine Core Research Institute (CRI) Kyung Hee University Seoul 02447 South Korea KHU-KIST Department of Converging Science and Technology Kyung Hee University Seoul 02447 South Korea UC San Diego Materials Research Science and Engineering Center La Jolla 92093 CA United States

Porous silicon nanoparticles (pSiNPs) have garnered significant attention for various applications, including drug delivery and biosensing. Conventionally, their production has been restricted to small-scale synthesis, often resulting in nanoparticles of variable sizes, which has limited their commercialization and practical use. In this study, we present a novel method for the scalable production of pSiNPs on a gram scale in a relatively short time using internal force-controlled (IFC) sonication. IFC sonication ensures power adjustment (from 60 W to 300 W), short processing times (<3 h), high yield (63.0 ± 4.18 %), and high reproducibility. This method produces nanoparticles with a superior degree of uniformity and consistent sizes, thereby addressing size variation challenges observed in previous approaches. These advancements facilitate large-scale production and enhance the reproducibility and applicability of pSiNPs in various fields. © 2025 Elsevier B.V.

关键词： Homogenizer Large-scale fabrication Nanomaterials Porous silicon Ultrasonication

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TP53 loss creates therapeutic vulnerability in colorectal cancer (vol 30, pg 697, 2015)

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NATURE 2021年第7875期597卷 E6-E6页

作者： Liu, Yunhua Zhang, Xinna Han, Cecil Wan, Guohui Huang, Xingxu Ivan, Cristina Jiang, Dahai Rodriguez-Aguayo, Cristian Lopez-Berestein, Gabriel Rao, Pulivarthi H. Maru, Dipen M. Pahl, Andreas He, Xiaoming Sood, Anil K. Ellis, Lee M. Anderl, Jan Lu, Xiongbin Department of Cancer Biology The University of Texas MD Anderson Cancer Center Houston TX USA Department of Gynaecologic Oncology and Reproductive Medicine The University of Texas MD Anderson Cancer Center Houston USA Center for RNA Interference and Non-coding RNAs The University of Texas MD Anderson Cancer Center Houston TX USA Department of Experimental Therapeutics The University of Texas MD Anderson Cancer Center Houston TX USA School of Life Science and Technology ShanghaiTech University Shanghai China Department of Paediatrics Baylor College of Medicine Houston TX USA Department of Pathology The University of Texas MD Anderson Cancer Center Houston TX USA Heidelberg Pharma GmbH Ladenburg Germany Department of Biomedical Engineering The Ohio State University Columbus OH USA Department of Surgical Oncology The University of Texas MD Anderson Cancer Center Houston TX USA

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An Open Natural Language Processing (NLP) Framework for EHR-based Clinical Research: A Case Demonstration Using the National COVID Cohort Collaborative (N3C)

arXiv

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arXiv 2021年

作者： Liu, Sijia Wen, Andrew Wang, Liwei He, Huan Fu, Sunyang Miller, Robert Williams, Andrew Harris, Daniel Kavuluru, Ramakanth Liu, Mei Abu-El-rub, Noor Schutte, Dalton Zhang, Rui Rouhizadeh, Masoud Osborne, John D. He, Yongqun Topaloglu, Umit Hong, Stephanie S. Saltz, Joel H. Schaffter, Thomas Pfaff, Emily Chute, Christopher G. Duong, Tim Haendel, Melissa A. Fuentes, Rafael Szolovits, Peter Xu, Hua Liu, Hongfang Department of Artificial Intelligence and Informatics Mayo Clinic United States Tufts Clinical and Translational Science Institute Tufts Medical Center United States Department of Internal Medicine University of Kentucky United Kingdom Department of Internal Medicine University of Kansas Medical Center United States Department of Pharmaceutical Care & Health Systems University of Minnesota Twin Cities United States Department of Pharmaceutical Outcomes & Policy University of Florida United States Department of Computer Science University of Alabama at Birmingham United States University of Michigan Medical School United States Wake Forest Baptist Medical Center United States Department of Medicine Johns Hopkins University United States Department of Biomedical Informatics Stony Brook University United States Sage Bionetwork United States Department of Medicine University of North Carolina Chapel Hill United States Albert Einstein College of Medicine United States Center for Health AI University of Colorado Anschutz Medical Campus United States Alex Informatics United States Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology United States School of Biomedical Informatics University of Texas Health Science Center Houston United States

Despite recent methodology advancements in clinical natural language processing (NLP), adoption of clinical NLP models within the clinical and translational research community remains hindered by issues with ETL process heterogeneity and human factor variations. In this study, we proposed an open NLP development framework with the aim of addressing these issues. The viability of such a platform was evaluated on a COVID-19 use case through sites participating in the National COVID Cohort Collaborative (N3C). As part of our assessment of the impact of single vs. multi-site NLP algorithm development, we evaluated the performance of both an NLP ruleset developed solely using a single site’s clinical narratives as well as one further refined using a synthetic derived dataset sourced from three sites (Mayo, UKen, and UMN). The single-site ruleset resulted in performances of 0.876, 0.706, and 0.694 in F-scores for Mayo, Minnesota, and Kentucky test datasets, respectively, while the multi-site NLP ruleset improved performances to 0.884, 0.769 and 0.806. The results of our use case test run inform us of the importance of a multisite federated development, evaluation, and implementation framework. As such, we aim to meet this need with our framework by providing the tools necessary to conduct NLP development in a collaborative manner through consensus building, process coordination, and best practice sharing. © 2021, CC BY.

关键词： Natural language processing systems

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Potent bivalent nanobody constructs that protect against the SARS-CoV-2 XBB variant

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Npj viruses 2025年第1期3卷 19页

作者： Peter J Halfmann Jeong Soo Lee Nikki McArthur Ojas Gupta Yoshihiro Kawaoka Ravi S Kane Department of Pathobiological Sciences Influenza Research Institute School of Veterinary Medicine University of Wisconsin Madison WI USA. School of Chemical & Biomolecular Engineering Georgia Institute of Technology Atlanta GA USA. Wallace H. Coulter Department of Biomedical Engineering Georgia Institute of Technology Atlanta GA USA. Department of Pathobiological Sciences Influenza Research Institute School of Veterinary Medicine University of Wisconsin Madison WI USA. yoshihiro.kawaoka@wisc.edu. Division of Virology Department of Microbiology and Immunology Institute of Medical Science University of Tokyo Tokyo Japan. yoshihiro.kawaoka@wisc.edu. The Research Center for Global Viral Diseases National Center for Global Health and Medicine Research Institute Tokyo Japan. yoshihiro.kawaoka@wisc.edu. Pandemic Preparedness Infection and Advanced Research Center (UTOPIA) University of Tokyo Tokyo Japan. yoshihiro.kawaoka@wisc.edu. School of Chemical & Biomolecular Engineering Georgia Institute of Technology Atlanta GA USA. ravi.kane@chbe.gatech.edu. Wallace H. Coulter Department of Biomedical Engineering Georgia Institute of Technology Atlanta GA USA. ravi.kane@chbe.gatech.edu. Parker H. Petit Institute for Bioengineering and Bioscience Georgia Institute of Technology Atlanta GA USA. ravi.kane@chbe.gatech.edu.

Most antibody-based therapeutics approved for SARS-CoV-2 treatment have shown greatly reduced neutralization activity against emerging Omicron variants. To target recent Omicron variants, we developed XBB-specific antibody-like therapeutics by screening a yeast surface-displayed single-domain antibody library against the receptor binding domain of the XBB spike protein. Three lead nanobodies, XNb 4.13, XNb 4.14, and XNb 4.15, were selected based on their binding affinity to the XBB spike protein and were fused to a mouse Fc domain. While all three constructs showed sub-nanomolar binding affinities to the XBB S protein, XNb 4.13-Fc and XNb 4.14-Fc also neutralized XBB in vitro. Intraperitoneal injection of XNb 4.13-Fc and XNb 4.14-Fc and intranasal delivery of XNb 4.13-Fc protected transgenic mice from a challenge with XBB virus with a significant reduction in viral lung titers post-infection. These antibody-like constructs identified using yeast surface display have potential as therapeutics that can protect against SARS-CoV-2 XBB variants.

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