检索结果-内蒙古大学图书馆

Improving algorithm for the alignment of consecutive, whole-slide, immunohistochemical section Images

Journal of Pathology Informatics 2021年第1期12卷 29页

作者： Liang, Cher-Wei Chang, Ruey-Feng Fang, Pei-Wei Chen, Chiao-Min Department of Pathology Fu Jen Catholic University Hospital Fu Jen Catholic University New Taipei City Taiwan School of Medicine College of Medicine Fu Jen Catholic University New Taipei City Taiwan Department and Graduate Institute of Pathology National Taiwan University Hospital and National Taiwan University College of Medicine Taipei Taiwan Department of Computer Science and Information Engineering National Taiwan University Taipei Taiwan Graduate Institute of Biomedical Electronics and Bioinformatics National Taiwan University Taipei Taiwan Most Joint Research Center for Ai Technology and All Vista Healthcare Taipei Taiwan

Background: Accurate and precise alignment of histopathology tissue sections is a key step for the interpretation of the proteome topology and cell level three-dimensional (3D) reconstruction of diseased tissues. However, the realization of an automated and robust method for aligning nonglobally stained immunohistochemical (IHC) sections is still challenging. In this study, we aim to assess the feasibility of multidimensional graph-based image registration on aligning serial-section and whole-slide IHC section images. Materials and Methods: An automated, patch graph-based registration method was established and applied to align serial, whole-slide IHC sections at ×10 magnification (average 32,947 × 27,054 pixels). The alignment began with the initial alignment of high-resolution reference and translated images (object segmentation and rigid registration) and nonlinear registration of low-resolution reference and translated images, followed by the multidimensional graph-based image registration of the segmented patches, and finally, the fusion of deformed patches for inspection. The performance of the proposed method was formulated and evaluated by the Hausdorff distance between continuous image slices. Results: Sets of average 315 patches from five serial whole slide, IHC section images were tested using 21 different IHC antibodies across five different tissue types (skin, breast, stomach, prostate, and soft tissue). The proposed method was successfully automated to align most of the images. The average Hausdorff distance was 48.93 μm with a standard deviation of 14.94 μm, showing a significant improvement from the previously published patch-based nonlinear image registration method (average Hausdorff distance of 93.89 μm with 50.85 μm standard deviation). Conclusions: Our method was effective in aligning whole-slide tissue sections at the cell-level resolution. Further advancements in the screening of the proteome topology and 3D tissue reconstruction could be expe

关键词： Cell-level resolution Image registration Immunohistochemistry Multidimensional graph-based registration Proteome topology Three-dimensional tissue reconstruction Whole-slide images

来源：评论

学校读者我要写书评

暂无评论

380P Positron emission tomography prognostic utility in HER2-positive advanced breast cancer patients starting treatment with TPH regimen

引用

ESMO Open 2025年 10卷 104951-104951页

作者： M. Kubeczko A. d'Amico O. Chrabański M. Mianowska-Malec K. Swiderska A. Polakiewicz-Gilowska A. Lesniak B. Lanoszka K. Chomik B.D. Grandys N. Lisovska E. Stobiecka J. Simek B. Pycinski D. Handkiewicz Junak E. Chmielik A. Prat M. Jarzab Breast Cancer Center Maria Sklodowska-Curie National Research Institute of Oncology Gliwice Poland Department of Nuclear Medicine and Endocrine Oncology National Oncology Institute Maria Sklodowskiej-Curie National Research Institute Gliwice Branch Gliwice Poland Tumor Pathology Department Maria Sklodowska-Curie National Research Institute of Oncology Gliwice Poland Faculty of Biomedical Engineering Silesian University of Technology Zabrze Poland Department of Nuclear Medicine and Endocrine Oncology Maria Sklodowska-Curie National Research Institute of Oncology Gliwice Poland Translational Genomics and Targeted Therapies in Solid Tumors IDIBAPS - Fundació de Recerca Clinic Barcelona - Institut d'Investigacions Biomediques August Pi i Sunyer Barcelona Spain

来源：评论

学校读者我要写书评

暂无评论

Dual Stimuli-Responsive MOF-Based Nanosystem for Synergistic Photothermal/Pharmacological Antibacterial Therapy

SSRN

引用

SSRN 2020年

作者： Xiao, Ya Xu, Mengran Lv, Na Cheng, Chen Huang, Pei Li, Jiabin Hu, Yi Sun, Ming Department of Stomatology Anhui Medical University Hefei230022 China Department of Infectious Diseases Anhui Medical University Hefei230022 China Hefei National Lab for Physical Sciences at the Microscale and the Centers for Biomedical Engineering University of Science and Technology of China Hefei230027 China Anhui Center for Surveillance of Bacterial Resistance Institute of Bacterial Resistance Anhui Medical University Department of Infectious Diseases Anhui Medical University Hefei China

The serious threat of drug-resistant bacterial pathogens has arisen through overuse of antibiotics. Photothermal therapy (PTT) has come to prominence as viable alternative strategy for antibacterial therapy. In this work, we report a NIR/pH dual stimuli-responsive antibacterial formulation based on zeolitic imidazolate frameworks-8 (ZIF-8) with strong antibacterial activity that combines photothermal heating with enhanced antibiotic delivery. ZIF-8 with polydopamine (PDA) surface modification was used to encapsulate the antibiotic vancomycin to construct a novel antimicrobial formulation (Van@ZIF-8@PDA). This treatment was tested against Gram-positive vancomycin-resistant Staphylococcus aureus Mu50. Results showed that the PDA coating improved ZIF-8 stability and dispersion, while also conferring a high photothermal conversion efficiency. Hyperthermia activated by near-infrared (NIR) light irradiation, in conjunction with pH-dependent nanoparticle degradation to release vancomycin, enabled tight control of drug delivery that functioned synergistically in the elimination of both planktonic bacteria prior to biofilm formation and established biofilms. We found that this combined formulation compromises cell structure while also degrading bacterial DNA. Moreover, further investigation showed that the Van@ZIF-8@PDA nanoparticles exhibit excellent biocompatibility, with low toxicity toward host organs and tissues, while also reducing the antibiotic concentration needed for effective bacterial control. Finally, we treated Mu50 in a mouse model of skin abscess and found that Van@ZIF-8@PDA was effective and safe in vivo. Cumulatively, this study shows that this NIR/pH dual stimuli-responsive nanoparticle-based formulation offers a promising potential strategy for clinical application against bacterial infection that circumvents antibiotic resistance. © 2020, The Authors. All rights reserved.

关键词： Antibiotics

来源：评论

学校读者我要写书评

暂无评论

ATP6V0d2 mediates leucine-induced mTORCI activation and polarization of macrophages

引用

Protein & Cell 2019年第8期10卷 615-619页

作者： Pingfei Li Xiaofei Deng Jing Luo Yufei Chen Guoyu Bi Feili Gong Zhengping Wei Na Liu Huabin Li Arian Laurence Xiang-Ping Yang Department of Immunology School of Basic Medicine Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China Department of Immuno logy School of Basic Medicine Hubei University of Medicine Shiyan 442000 China The First Affiliated Hospital Biomedical Translational Research Institute and Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering Jinan University Guangzhou 510632 China Department of Otolaryngology-Head and Neck Surgery Center for Allergic and Inflammatory Diseases Affiliated Eye and ENT Hospital Fudan University Shanghai 200031 China Department of Haematology University College London Hospitals NHS trust London UK

Dear Editor, mTORCI, as a center regulatory hub of metabolism, senses the cellular energy status, nutrition and extracellular stimuli and regulates cell growth, differentiation and functions of immune cells (Powell et al., 2012). Lysosomal localization of key signal comp orients is critical for mTORCI activati on: mTORCI activation requires co-localization of activated Rheb and mTORCI to the lysosome membrane (Buerger et al., 2006). Signals in eluding growth factors, cellular stresses and energy levels act on the disruption the formation of tuberous sclerosis complex (TSC) complex, comprised of TSC1, TSC2 and TBC1D7, which leads to the translocation and activation of Rheb on the lysosome membrane (Dibble et al., 2012). In response to nutrient levels, specifically the availability of amino acids and glucose (Efeyan et al., 2013), mTORCI is recruited to the lysosomal surface by Rag GTPases that are heterodimers of RagA or RagC bound to RagB or RagD. Multiple protein complexes have been implicated in regulation of mTORCI upon nutrient sensing including Ragulator, GATOR1, GATOR2, KICSTOR and vacuolar ATPases (Wolfson et al., 2017). Vacuolar ATPases are large multiple-protein complexes that acidify the lysosome and may mediate additional functions independent of their proton pump activity (Nishi and Forgac, 2002).

关键词： ATP6V0d2 mediates leucine-induced mTORCI activation macrophages

来源：评论

学校读者我要写书评

暂无评论

Enhanced electrochemical immunosensor utilizing pATA/AuNPs for sensitive detection of the leukemia-associated biomarker CD123 in bone marrow supernatant

引用

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 2025年 211卷 107124页

作者： Xin Li Qing Wang Chun-Rong Qin Ai-Lin Liu Yong Chen Department of Hematology Fujian Institute of Hematology Fujian Provincial Key Laboratory on Hematology Fujian Medical University Union Hospital 350001 Fuzhou China Department of Blood Transfusion Medicine School of Medical Technology and Engineering Fujian Medical University Fuzhou350122 China. Department of Histology and Embryology School of Basic Medical Sciences Fujian Medical University Fuzhou 350122 China Sichuan Nursing Vocational College Chengdu 610100 China. Center for Reproductive Medicine Shenzhen Maternity and Child Healthcare Hospital Shenzhen 518000 China. Department of Pharmaceutical Analysis Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province School of Pharmacy Fujian Medical University 350122 Fuzhou China. Department of Histology and Embryology School of Basic Medical Sciences Fujian Medical University Fuzhou 350122 China. Electronic address: fjmucy@***.

BACKGROUND:Cluster of differentiation 123 (CD123) prominently overexpress in various hematological malignancies and plays a crucial role in diagnosis and prognosis of leukemia. Clinical studies have demonstrated that cell-free CD123 levels also significantly influence leukemia immunotherapy outcomes. The development of novel electrochemical immunosensors addresses the need for point-of-care detection tools, thereby facilitating advancements in clinical monitoring technologies. METHODS:This study presented a practical electrochemical immunosensor constructed using sandwich strategy for CD123 detection based on the modification of gold nanoparticles and poly(2-aminoterephthalic acid). Common proteins and tumor-related biomarkers found in human were selected as interference factors to evaluate the detection specificity of the electrochemical immunosensor. Further, the electrochemical immunosensor was utilized to directly detect CD123 in bone marrow supernatant from leukemia patients. RESULTS:The constructed electrochemical immunosensor exhibited good linearity for CD123 detection over a range of 0.02 to 2.5 µg/mL, with a detection limit of 12.8 ng/mL, alongside satisfactory specificity and repeatability. Furthermore, the immunosensor was successfully employed to detect CD123 levels in the bone marrow supernatant of leukemia patients, demonstrating results that were highly consistent with those obtained via ELISA. CONCLUSIONS:The developed approach is anticipated to provide robust technical support for the long-term monitoring of leukemia patients during their diagnosis and treatment.

关键词： Bone marrow supernatant CD123 Electrochemical immunosensor Leukemia Poly(2-aminoterephthalic acid)

来源：评论

学校读者我要写书评

暂无评论

Induction of apoptosis by cold atmospheric pressure plasma for oral squamous cell carcinoma cells

引用

Plasma Medicine 2018年第4期8卷 411-418页

作者： Ramireddy, Latha Lai, Chih Ho Low, Bih Show Li, Chuan Hsieh, Jang Hsing Lee, Jyh Wei Wu, Hui Yu Center for Plasma and Thin Film Technologies Ming Chi University of Technology New Taipei City Taishan Taiwan Department of Microbiology and Immunology Chang Gung University Taoyuan Taiwan Center for General Education Chang Gung University Taoyuan Taiwan Department of Biomedical Engineering National Yang Ming University Taipei Taiwan Department of Materials Engineering Ming Chi University of Technology New Taipei City Taishan Taiwan

Cold atmospheric pressure plasma (CAPP) techniques have developed rapidly during the last decades and have become an important field in plasma medicine. Plasma is ionized gas that is comprised of reactive atoms, molecules, ions, and radicals. CAPP generates various types of highly reactive oxygen and nitrogen species at room temperature that play an important part in biological applications including cancer therapy. In recent years, CAPP has gained increasingly more interest among cancer researchers, but the mechanism of CAPP’s effect is not fully understood. The aim of this study is to investigate both the mechanism of CAPP on oral squamous cell carcinoma (OSCC) cells as well as plasma-induced radicals. We observe plasma-induced highly reactive species in a plasma plume and cell culture. A cell proliferation assay shows that CAPP significantly decreases proliferation of oral squamous cells in vitro. Western blot analysis shows cleavage of poly (ADP-ribose) polymerase protein after plasma treatment, indicating that CAPP can induce cell death via the apoptosis pathway. These findings show that CAPP could be used as an alternative adjuvant therapeutic tool for the treatment of OSCC. © 2018 by Begell House, Inc.

关键词： Cell death

来源：评论

学校读者我要写书评

暂无评论

Author Correction: Cell-based versus corticosteroid injections for knee pain in osteoarthritis: a randomized phase 3 trial

引用

Nature medicine 2024年第3期30卷 905页

作者： Ken Mautner Michael Gottschalk Scott D Boden Alison Akard Won C Bae Lora Black Blake Boggess Paramita Chatterjee Christine B Chung Kirk A Easley Greg Gibson Josh Hackel Katie Jensen Linda Kippner Chad Kurtenbach Joanne Kurtzberg R Amadeus Mason Benjamin Noonan Krishnendu Roy Verle Valentine Carolyn Yeago Hicham Drissi Department of Orthopaedics Emory University Atlanta GA USA. kmautne@emory.edu. Department of Orthopaedics Emory University Atlanta GA USA. Department of Radiology University of California Davis La Jolla CA USA. Sanford Health Sioux Falls SD USA. Duke Sports Medicine Durham NC USA. Marcus Center for Therapeutic Cell Characterization and Manufacturing Georgia Institute of Technology Atlanta GA USA. Department of Biostatistics and Bioinformatics Emory University Atlanta GA USA. Center for Integrative Genomics Georgia Institute of Technology Atlanta GA USA. Andrews Institute Gulf Breeze AL USA. Marcus Center for Therapeutic Cures Duke University Durham NC USA. Sanford Health Fargo ND USA. Biomedical Engineering Vanderbilt University Nashville TN USA. Department of Orthopaedics Emory University Atlanta GA USA. hicham.drissi@emory.edu.

来源：评论

学校读者我要写书评

暂无评论

Multiscale Engineered Bionic Solid-State Electrolytes Breaking the Stiffness-Damping Trade-Off

引用

Angewandte Chemie 2025年第11期137卷

作者： Dr. Junyu Hou Wu Sun Qunyao Yuan Longjiang Ding Yanhua Wan Zuohui Xiao Tianke Zhu Xingyu Lei Jingsen Lin Rongrong Cheacharoen Yunlei Zhou Dr. Shaolei Wang Dr. Farid Manshaii Jin Xie Wei Li Jie Zhao Department of Materials Science State Key Laboratory of Molecular Engineering of Polymers Fudan University Shanghai 200438 P. R. China Beijing Advanced Innovation Center for Biomedical Engineering School of Chemistry Beihang University Beijing 100191 P. R. China Department of Chemistry Laboratory of Advanced Materials Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials State Key Laboratory of Molecular Engineering of Polymers and iChEM Fudan University Shanghai 200438 P. R. China Department of Oral and Maxillofacial Implantology Shanghai PerioImplant Innovation Center Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine College of Stomatology Shanghai Jiao Tong University National Center for Stomatology National Clinical Research Center for Oral Diseases Shanghai Key Laboratory of Stomatology Shanghai Research Institute of Stomatology Shanghai 200011 P. R. China Metallurgy and Materials Science Research Institute Chulalongkorn University Bangkok 10330 Thailand Hangzhou Institute of Technology Xidian University Hangzhou 311200 P. R. China Department of Bioengineering University of California Los Angeles Los Angeles California 90095 USA School of Physical Science and Technology & Shanghai Key Laboratory of High-resolution Electron Microscopy ShanghaiTech University Shanghai 201210 P. R. China

All-solid-state lithium metal batteries (LMBs) are regarded as next-generation devices for energy storage due to their safety and high energy density. The issues of Li dendrites and poor mechanical compatibility with electrodes present the need for developing solid-state electrolytes with high stiffness and damping, but it is a contradictory relationship. Here, inspired by the superstructure of tooth enamel, we develop a composite solid-state electrolyte composed of amorphous ceramic nanotube arrays intertwined with solid polymer electrolytes. This bionic electrolyte exhibits both high stiffness (Young′s modulus=15 GPa, hardness=0.13 GPa) and damping (tan δ =0.08), breaking the trade-off. Thus, this composite electrolyte can not only inhibit Li dendrites growth but also ensure intimate contact with electrodes. Meanwhile, it also exhibits considerable Li + transference number (0.62) and room temperature ionic conductivity (1.34×10 −4 S cm −1 ), which is attributed to oxygen vacancies of the amorphous ceramic effectively decoupling the Li-TFSI ion pair. Consequently, the assembled Li symmetric battery shows an ultra-stable cycling (>2000 hours at 0.1 mA cm −2 at 60 °C, >500 hours at 0.1 mA cm −2 at 30 °C). Moreover, the LiFePO 4 /Li and LiNi 0.8 Co 0.1 Mn 0.1 O 2 /Li all-solid-state full cells both show excellent cycling performance. We demonstrate that this bionic strategy is a promising approach for the development of high-performance solid-state electrolytes.

关键词： Solid-State Electrolytes Biomimetic Synthesis Amorphous Ceramic Arrays All-Solid-State Lithium Metal Batteries

来源：评论

学校读者我要写书评

暂无评论

Correction: In situ synthesis of chiral AuNCs with aggregation-induced emission using glutathione and ceria precursor nanosheets for glutathione biosensing

引用

Analyst 2022年第22期147卷 5248-5248页

作者： Mohamed Ibrahim Halawa Guoxing Wu Alaa Eldin A. Salem Lei Su Bing Shi Li Xueji Zhang College of Chemistry and Environmental Engineering Shenzhen University Shenzhen 518060 China Guangdong Laboratory of Artificial Intelligence & Digital Economy (SZ) Shenzhen University Shenzhen 518060 China College of Biomedical Engineering International Health Science Innovation Center Shenzhen Key Laboratory for Nano-Biosensing Technology Health Science Center Shenzhen University Shenzhen 518060 China Department of Pharmaceutical Analytical Chemistry Faculty of Pharmacy Mansoura University Mansoura Egypt Department of Chemistry College of Science United Arab Emirates University Al Ain United Arab Emirates

Correction for ‘In situ synthesis of chiral AuNCs with aggregation-induced emission using glutathione and ceria precursor nanosheets for glutathione biosensing’ by Mohamed Ibrahim Halawa et al., Analyst, 2022, https...

Correction for ‘In situ synthesis of chiral AuNCs with aggregation-induced emission using glutathione and ceria precursor nanosheets for glutathione biosensing’ by Mohamed Ibrahim Halawa et al., Analyst, 2022, https://***/10.1039/d2an00939k.

关键词：

来源：评论

学校读者我要写书评

暂无评论

Comprehensive histological imaging of native microbiota in human glioma

Research Square

引用

Research Square 2021年

作者： Zhao, Jiajia He, Dian Lai, Hei Ming Xu, Yingying Luo, Yunhao Li, Ting Liang, Jianhao Yang, Xiaodu Guo, Linlang Ke, Yiquan Zhou, Hongwei Wu, Wutian Guo, Hongbo Sun, Haitao Microbiome Medicine Center Clinical Biobank Center Department of Laboratory Medicine Zhujiang Hospital Southern Medical University Guangzhou510280 China Neurosurgery Center The National Key Clinical Specialty The Engineering Technology Research Center of Education Ministry of China on Diagnosis and Treatment of Cerebrovascular Disease Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration The Neurosurgery Institute of Guangdong Province Zhujiang Hospital Southern Medical University Guangzhou510280 China Department of Psychiatry Faculty of Medicine The Chinese University of Hong Kong Shatin Hong Kong School of Biomedical Engineering Guangdong Provincial Key Laboratory of Medical Image Processing Southern Medical University Guangzhou510515 China Department of Pathology Zhujiang Hospital Southern Medical University Guangzhou510280 China Jinan University Guangzhou510632 China Re-Stem Biotechnology Co. Ltd Suzhou215300 China Key Laboratory of Mental Health of the Ministry of Education Guangdong–Hong Kong–Macao Greater Bay Area Center for Brain Science and Brain-Inspired Intelligence Southern Medical University Guangzhou510515 China

Mounting evidence suggests that distinct microbial communities reside in tumors and play important roles in tumor physiology. Recently, Nejman et al. profiled the composition and localization of intratumoral bacteria using 16S DNA sequencing and histological visualization methods across seven tumor types, including human glioblastoma. However, considering potential contamination in their sample origins and processing, the results based on traditional histological methods need to be validated. Here, we propose a three-dimensional (3D) intratumoral microbiota visualization and quantification protocol to observe microbiota in intact tumor tissues on the premise of avoiding possible contamination in the surface of tissues, based on tissue clearing, immunofluorescent labeling, microscopy imaging, and image processing. For the first time, we have achieved 3D quantitative imaging of bacterial LPS fluorescent signals deep in gliomas in a contamination-free manner, which was founded mostly localized near nuclear membranes or in the intercellular space. Through an automated statistical algorithm, reliable signals can be distinguished for further analysis of their sizes, distribution, and fluorescence intensities. Combining two-dimensional images from multiple thin-section histological methods, including immunochemistry and fluorescence in situ hybridization, we provide a comprehensive histological investigation of the morphology and distribution of these signals on human glioma samples. We expect that this multi-evidence chain will provide supporting proof for the presence of intratumoral bacteria in human glioma and that the integrated pipeline can be applied to investigate the native bacteria within diverse tumors and contribute to the interpretation of their direct roles in the tumor microenvironment. © 2021, CC BY.

关键词： Tumors

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：