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Extraction, Characterization, and Biocompatibility Evaluation of Type I Collagen from Chicken Feet Skin

biomedical Materials and Devices 2025年 1-15页

作者： Zourazema, Sevda Zendeh, Zahra Aksun, Elif Tugce Gorgun, Busra Guzel, Fatma Dogan Cecen, Berivan Kozaci, Leyla Didem Department of Translational Medicine Institute of Health Sciences Ankara Yildirim Beyazit University Ankara 06800 Türkiye Department of Food Processing-Food Technology Vocational School of Health Services Ankara Yildirim Beyazit University Ankara 06010 Türkiye Faculty of Engineering and Natural Sciences Department of Biomedical Engineering Ankara Yildirim Beyazit University Ankara Türkiye Department of Biomedical Engineering Newark College of Engineering New Jersey Institute of Technology Newark 07102 NJ United States Stem Cell and Tissue Engineering Application and Research Center Istinye University Istanbul 34010 Türkiye Department of Medical Biochemistry Faculty of Medicine Ankara Yildirim Beyazit University Ankara 06800 Türkiye

Abstract: The study focused on extracting type I collagen, a protein derived from chicken feet skin, for applications in the biomedical and cosmetic industries. The research thoroughly explored the extraction methodology and characterization techniques and assessed the biocompatibility of the collagen. Acid extraction yielded substantial amounts of type I collagen with an efficiency of 11.35%. The characterization process involved various analyses, including FTIR, SDS-PAGE, western blot, and SEM imaging, to evaluate purity, structural integrity, and morphology. In vitro testing confirmed the biocompatibility of the extracted collagen, indicating its potential as a natural polymer for medical studies. The collagen extracted from chicken feet skin demonstrated significant bioactivity, positioning it as a promising polymer for tissue engineering, wound healing, and cosmetic applications. This study underlines the importance of developing biocompatible biomaterials and provides valuable insights into a sustainable source of collagen from animal by-products. © This is a U.S. Government work and not under copyright protection in the US;foreign copyright protection may apply 2024.

关键词： Biocompatibility Chicken foot skin Type I collagen extraction

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USP1 inhibits influenza A and B virus replication in MDCK cells by mediating RIG-I deubiquitination

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Cellular and Molecular Life Sciences 2025年第1期82卷 1-15页

作者： Liao, Yuejiao Wang, Siya Tang, Tian Li, Chengfan Yang, Chenhao Ma, Liyuan Ye, Jin Wang, Jiamin Yang, Di Qiao, Zilin Ma, Zhongren Liu, Zhenbin Engineering Research Center of Key Technology and Industrialization of Cell-Based Vaccine Ministry of Education Northwest Minzu University Lanzhou 730030 China Gansu Tech Innovation Center of Animal Cell Biomedical Research Center Northwest Minzu University Lanzhou 730030 China Key Laboratory of Biotechnology & Bioengineering of State Ethnic Affairs Commission Biomedical Research Center Northwest Minzu University Lanzhou 730030 China Life Science and Engineering College of Northwest Minzu University Lanzhou 730030 China Department of Experiment & Teaching Northwest Minzu University Lanzhou 730030 China

The post-translational modification and stability regulation of RIG-I play critical roles in promoting IFN-I production and maintaining immune homeostasis. In this study, we found that ubiquitin-specific peptidase 1 (USP1) promotes RIG-I protein stability through deubiquitination, which in turn enhances antiviral immunity through the production of inflammatory cytokines, and inhibits the replication of influenza virus in MDCK cells. In contrast, USP1 knockdown inhibited the deubiquitination of RIG-I, decreased the RIG-I protein level, and significantly increased the influenza virus titer. Meanwhile, inhibition of USP1 expression did not have a significant effect on the proliferation of MDCK cells, suggesting that USP1 could be used as a target gene to establish a vaccine-producing MDCK cell line. The above results provide a more comprehensive understanding of the function of USP1 and the antiviral response mechanism, and provide a theoretical and methodological basis for the screening of target genes for the artificial establishment of high-yield MDCK cell lines for vaccine production. © The Author(s) 2025.

关键词： Influenza virus Innate immunity MDCK cells RIG-I USP1 Vaccine production

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Skeletal Muscle Oxygen Consumption Dynamic Features Base on Near-Infrared Spectroscopy 6th

Skeletal Muscle Oxygen Consumption Dynamic Features Base on ...

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6th International Conference on biomedical and Health Informatics, ICBHI 2024

作者： Lu, Shao-Hung Fu, Tieh-Cheng Tsai, Cheng-Lun Lin, Kang-Pin Technology Translation Center for Medical Device Chung Yuan Christian University Taoyuan Taiwan Department of Physical Medicine and Rehabilitation Chang Gung Memorial Hospital Keelung Taiwan Biomedical Engineering Department of Chung Yuan Christian University Taoyuan Taiwan Electrical Engineering Department Chung Yuan Christian University Taoyuan Taiwan

ISBN: (纸本)9783031863226

This study utilized Near-Infrared Spectroscopy (NIRS) to assess the oxygen metabolism characteristics of lower limb skeletal muscles during ankle pumping exercises, simulating short-duration, high-intensity dynamic exercise. Four NIRS probes were used to simultaneously measure the bilateral medial gastrocnemius muscles. Data were collected from 20 healthy subjects over two sessions of ankle pumping exercises, including measurements of oxygenated, deoxygenated, and total hemoglobin. The results showed that oxygen metabolism during the second exercise session (2.24 ± 0.88 µM) was consistently higher than during the first (26.56 ± 14.36 µM), likely due to a post-exercise effect. During the second session, the blood flow response in the medial gastrocnemius was classified into three distinct metabolic patterns: an increase, stabilization, or decrease in total hemoglobin levels. Among these, an increase in total hemoglobin during exercise was the most common, observed in 64% of cases, possibly resulting from endothelial-related vasodilation induced by exercise. The variations in NIRS measurements within the same muscle are hypothesized to be due to the differing compositions of arteries, veins, and microvessels through which the light passes. Therefore, when using NIRS to evaluate dynamic tissue oxygen metabolism and hemodynamic physiological responses, careful consideration should be given to factors such as the measurement location within the muscle, the initial state of the muscle tissue, the warm-up duration and intensity before exercise, and the baseline metabolic characteristics prior to the exercise session. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2025.

关键词： Near infrared spectroscopy

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Microneedle delivery platform integrated with Staphylococcus epidermidis-derived extracellular vesicles-based nanoantibiotics for efficient bacterial infection atopic dermatitis treatment

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Acta Pharmaceutica Sinica B 2025年第4期15卷 2197-2216页

作者： Zhou, Hong Zhang, Shuting Liu, Xinxin Feng, Aiping Chen, Siyuan Liu, Wei National Engineering Research Center for Nanomedicine College of Life Science and Technology Huazhong University of Science and Technology Wuhan 430074 China Department of Dermatology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430074 China Research Institute for Biomaterials Tech Institute for Advanced Materials Bioinspired Biomedical Materials & Devices Center College of Materials Science and Engineering Jiangsu Collaborative Innovation Center for Advanced Inorganic Function Composites Suqian Advanced Materials Industry Technology Innovation Center Nanjing Tech University Nanjing 211816 China

Due to the difficulty of overcoming the abnormal epidermal barriers and addressing S. aureus infections without disrupting indigenous skin microbiota, effective treatment of bacterial infection atopic dermatitis (AD) remains a significant clinical challenge. Skin microbiota-derived extracellular vesicles (EVs) shows protentional for skin disease treatment, but the lack of antimicrobial activity and limited skin penetration hamper their application in bacterial infection AD treatment. Here, we developed novel nanoantibiotics by loading Lev into S. epidermidis-derived EVs (Lev@SE-EVs), with supreme antimicrobial activity, regulating epidermal immune responses and enhanced epidermal barrier functionality. The nanoantibiotics were further integrated into hyaluronic acid-based microneedle (MN) for efficient transdermal delivery of therapeutic agents and effectively treating bacterial infection in AD. Upon insertion into the skin, the rapidly released Lev@SE-EVs from MN are uptake by S. aureus in a selective manner, fibroblasts, and surrounding immune cells to exert therapeutic effects in the infected dermal layer, resulting in mitigated skin inflammation, reduced S. aureus burden and increased dermis repair. Notably, Lev@SE-EVs induce IL-17A⁺ CD8⁺ T-cell accumulation in the skin in an unrelated inflammation manner, which may represent heterologous protection. This EVs-integrated MN assisted Lev@SE-EVs to alleviate skin inflammation, repair skin, and provide an effective and safe therapeutic approach for bacterial infection AD treatment. © 2025 The Authors

关键词： Atopic dermatitis Bacterial infection Extracellular vesicles Microneedle Nanoantibiotics Skin microbiota Staphylococcus aureus Staphylococcus epidermidis

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Editors-in-Chief Roundtable Discussion: Towards Improving Quality of Journal Papers

Transactions of Japanese Society for Medical and Biological ...

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Transactions of Japanese Society for Medical and Biological engineering 2025年第1期63卷 65-72页

作者： Fukuoka, Yutaka Ono, Yumie Yokosawa, Koichi Kawada, Toru Nakamura, Kazuhiro Maeda, Yoshinobu Nishikawa, Atsushi Kasahara, Masahiko School of Engineering Kogakuin University Japan School of Science and Technology Meiji University Japan Faculty of Health Sciences Hokkaido University Japan Department of Cardiovascular Dynamics National Cerebral and Cardiovascular Center Japan Research Institute for Brain and Blood Vessels Akita Japan Graduate School of Science and Technology Niigata University Japan Graduate School of Engineering Science The University of Osaka Japan Editorial Office of the Advanced Biomedical Engineering and the Transactions of Japanese Society for Medical and Biological Engineering Japan

To improve the quality of journal papers in the Advanced biomedical engineering as well as the Transactions of Japanese Society for Medical and Biological engineering, the Editors-in-Chief held roundtable discussions on various issues including initial checks on submitted manuscripts (ethical issues, COI, and recommendations by the International Committee of Medical Journal Editors), improving the template files and the submission system, originality criteria, journal impact factor, article publishing charge (APC) and J-STAGE Data, etc. The editors also discussed future plans for educational seminars and articles. By publicizing editorial policies including the above issues to society members, the editorial committee aims to further improve the quality of journal papers and to enhance the journals’ presence. © 2025, Japan Soc. of Med. Electronics and Biol. engineering. All rights reserved.

关键词： Biochemical engineering

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An "on–off-on" photoelectrochemical aptasensor using CoO as a signal label for T-2 toxin detection

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Microchimica Acta 2025年第6期192卷 1-11页

作者： Shang, Keshuai Shan, Mengzhen Wang, Le Yu, Xiaolin Xu, Rui Jiang, Chenyu Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences Suzhou215163 China Jinan Guoke Medical Technology Development Co. Ltd Jinan250101 China Physical Education Department Shandong Traditional Chinese Medicine University Jinan250355 China Jinan Science and Technology Innovation Promotion Center Jinan250014 China

T-2 toxin, a mycotoxin commonly found in food, is recognized as one of the most harmful contaminants. Herein, a sensitive photoelectrochemical (PEC) aptasensor based on an "on–off-on" signal response strategy was developed for T-2 detection using WO3/CdIn2S4 as a photoanode. The sensitization of WO3 with CdIn2S4 significantly enhanced the photocurrent, leading to the initial "signal-on" state. As a signal label of probe DNA (pDNA), CoO significantly inhibited the photocurrent response of WO3/CdIn2S4 ("signal-off" state), enhancing the signal recovery space and improving the detection sensitivity. T-2 toxin is preferentially bound to aDNA, releasing the CoO-pDNA complex from the electrode, realizing the "signal-on" state again. This signal switching mechanism enabled a broad detection range from 1 fg mL–1 to 1 μg mL–1 with an ultralow detection limit of 0.434 fg mL–1 (S/N = 3), while the sensor exhibited excellent reproducibility, stability and selectivity. This platform not only provided a robust analytical tool for T-2 toxin detection in food safety but also established a generalizable sensing paradigm adaptable to other mycotoxins by replacing the recognition element. © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2025.

关键词： Image reconstruction

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Exploring the relationship between the gut microbiota and cognitive function in schizophrenia patients with distinct weights

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Schizophrenia Research 2025年 280卷 103-113页

作者： Zhu, Baoyuan Liang, Liqin Huang, Yuanyuan Wang, Haiyuan Zhou, Jing Xiong, Dongsheng Li, Shaochuan Li, Hehua Li, Xiaobo Chen, Shuhao Ning, Yuping Wu, Fengchun Wu, Kai School of Materials Science and Engineering South China University of Technology Guangzhou 510006 China School of Biomedical Sciences and Engineering South China University of Technology Guangzhou International Campus Guangzhou 511442 China Department of Psychiatry The Affiliated Brain Hospital Guangzhou Medical University Guangzhou 510370 China Guangdong Engineering Technology Research Center for Translational Medicine of Mental Disorders Guangzhou 510370 China National Engineering Research Center for Tissue Restoration and Reconstruction South China University of Technology Guangzhou 510006 China School of Biology and Biological Engineering South China University of Technology Guangzhou 510006 China Department of Biomedical Engineering New Jersey Institute of Technology Newark NJ United States Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China Guangzhou Medical University Guangzhou 510370 China Department of Aging Research and Geriatric Medicine Institute of Development Aging and Cancer Tohoku University Sendai 980-8575 Japan

Background: The gut microbiota is disrupted in schizophrenia (SZ) patients and is associated with cognitive function. This study aimed to investigate the gut microbiota composition in SZ patients with different body mass index (BMI) levels and their associations with cognitive function. Methods: We analyzed 16S rRNA sequencing data from 156 SZ patients, including 88 with overweight/obesity (OW) and 68 with normal weight (NW), and 156 normal control (NC), including 48 with OW and 108 with NW. We analyzed differences in microbial diversity and gut microbiota composition between SZ patients and NC at different BMI levels. Additionally, we explored the correlations between microbial communities, and symptom severity, as well as cognitive function. Furthermore, we examined between-group differences in metabolic pathways. Results: The abundance of Turicibacter was higher in the SZ_OW group but lower in the SZ_NW group compared to the NC groups at the same BMI level, respectively. In the SZ_OW group, increased Collinsella was significantly negatively associated with cognitive function, whereas decreased Clostridium and Butyricicoccus were significantly positively associated with cognitive function. Additionally, the functional analysis revealed enrichment of “metabolism of other amino acids” and “neurodegenerative disease” pathways, associated with non-standard amino acid metabolism and oxidative stress in the SZ_OW group compared to the NC_OW group. Conclusions: Our findings revealed significant differences in the gut microbiota between SZ patients and NC with different BMI levels and identified microbial associations with clinical characteristics, providing new insights into the mechanism of how the gut microbiota could impact cognitive deficits in SZ patients with obesity. © 2025

关键词： 16S rRNA Cognitive function Gut microbiota Obesity Schizophrenia

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Injectable chitosan microspheres resisting inflammatory and oxidativestress for ameliorating intervertebral disc degeneration

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Science China Materials 2025年第2期68卷 610-625页

作者： Lin Huang Wantao Wang Lei Liu Wenzheng Ma Jinghao Fan Dan Zhou Lei Zhao Zhaomin Zheng Hongmei Liu Decheng Wu Guangdong Provincial Key Laboratory of Advanced Biomaterials Department of Biomedical EngineeringSouthern University of Science and TechnologyShenzhen 518055China Department of Spine Surgery The First Affiliated HospitalPain Research CenterSun Yat-sen UniversityGuangzhou 510080China

The treatment of intervertebral disc (IVD) degeneration remains a significant challenge due to the uniqueischemic structure of the IVD, which comprises the scavengingof inflammatory cytokines, alleviation of cellular oxidativestress responses, restoration of nuclei pulposus (NP) cell viability,and recovery of IVD biomechanical function. Herein,we developed an injectable microsphere (CS-MnO_(2)@PC) byincorporating chitosan microspheres (CS) with manganesedioxide (MnO_(2)) nanozymes and celecoxib encapsulated inPluronic F-127 (PC) nanosized micelles, via in situ redox orSchiff base reaction. The hybrid carrier demonstrates robustcapabilities in scavenging free radicals, alleviating extracellularoxidative stress, and reducing inflammatory cytokinesin NP cells, as evidenced by RT-qPCR and immunofluorescence staining assays. In vivo evaluations further indicatethat this hybrid carrier helps preserve NP hydration andthe lamellar structure of the annulus fibrosus (AF), as confirmed by radiological analysis and histological staining *** injectable chitosan microspheres, combiningnanozymes and nanosized drug micelles, represent a promising therapeutic strategy for degenerative IVD.

关键词： chitosan microsphere nanozymes inflammatory relieving oxidative stress regulation intervertebral disc degeneration

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Swelling and erosion assisted sustained release of tea polyphenol from antibacterial ultrahigh molecular weight polyethylene for joint replacement

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Chinese Chemical Letters 2025年第2期36卷 316-320页

作者： Yue Ren Kang Li Yi-Zi Wang Shao-Peng Zhao Shu-Min Pan Haojie Fu Mengfan Jing Yaming Wang Fengyuan Yang Chuntai Liu State Key Laboratory of Structural Analysis Optimization and CAE Software for Industrial EquipmentNational Engineering Research Center for Advanced Polymer Processing TechnologyZhengzhou UniversityZhengzhou 450002China Tianjian Laboratory of Advanced Biomedical Sciences Academy of Medical SciencesZhengzhou UniversityZhengzhou 450000China Department of Stomatology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450000China

The considerable hazard posed by periprosthetic joint infections underlines the urgent need for the rapid advancement of in-situ drug delivery systems within joint ***,the pursuit of sustained antibacterial efficacy remains a formidable *** this context,we proposed a novel strategy that leverages swelling and erosion mechanisms to facilitate drug release of drug-loaded ultrahigh molecular weight polyethylene(UHMWPE),thereby ensuring its long-lasting antibacterial *** oxide(PEO),a hydrophilic polymer with fast hydrating ability and high swelling capacity,was incorporated in UHMWPE alongside the antibacterial tea polyphenol(epigallocatechin gallate,EGCG as representative).The swelling of PEO enhanced water infiltration into the matrix,while the erosion of PEO balanced the release of the encapsulated EGCG,resulting in a steady *** behavior was supported by the EGCG release profiles and the corresponding fitted release kinetic *** demonstrated by segmented antibacterial assessments,the antibacterial efficiency was enhanced 2to 3 times in the PEO/EGCG/UHMWPE composite compared to that of EGCG/***,the PEO/EGCG/UHMWPE composite exhibited favorable biocompatibility and mechanical performance,making it a potential candidate for the development of drug-releasing joint implants to combat prosthetic bacterial infections.

关键词： Tea polyphenol Antibacterial PEO UHMWPE Joint replacement

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Deciphering the toxic effects of polystyrene nanoparticles on erythropoiesis at single-cell resolution

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Zoological Research 2025年第1期46卷 165-176页

作者： Eun Jung Kwon Hyeon Mi Sung Hansong Lee Soyul Ahn Yejin Kim Chae Rin Lee Kihun Kim Kyungjae Myung Won Kyu Kim Dokyoung Kim Sanghwa Jeong Chang-Kyu Oh Yun Hak Kim Medical Research Institute Pusan National UniversityYangsanGyeongsangnam-do 50612Republic of Korea Institute for Future Earth Pusan National UniversityBusan 46241Republic of Korea Department of Convergence Medical Sciences School of MedicinePusan National UniversityYangsanGyeongsangnam-do 50612Republic of Korea Department of Biomedical Informatics School of MedicinePusan National UniversityYangsanGyeongsangnam-do 50612Republic of Korea Department of Anatomy School of MedicinePusan National UniversityYangsanGyeongsangnam-do 50612Republic of Korea Center for Genomic Integrity Institute for Basic ScienceUlsan 689-798Republic of Korea Department of Biomedical Engineering College of Information-Bio Convergence EngineeringUlsan National Institute of Science and TechnologyUlsan 44919Republic of Korea Natural Product Research Center Korea Institute of Science and Technology(KIST)GangneungGangwon-do 25451Republic of Korea Department of Convergence Medicine Yonsei University Wonju College of MedicineWonjuGangwon-do 26426Republic of Korea Natural Product Applied Science KIST SchoolUniversity of Science and Technology(UST)GangneungGangwon-do 25451Republic of Korea Department of Biomedical Science Graduate SchoolKyung Hee UniversitySeoul 02447Republic of Korea Department of Anatomy and Neurobiology College of MedicineKyung Hee UniversitySeoul 02447Republic of Korea Department of Precision Medicine Graduate SchoolKyung Hee UniversitySeoul 02447Republic of Korea Center for Converging Humanities Kyung Hee UniversitySeoul 02447Republic of Korea KHU-KIST Department of Converging Science and Technology Kyung Hee UniversitySeoul 02447Republic of Korea UC San Diego Materials Research Science and Engineering Center 9500 Gilman DriveLa JollaCalifornia 92093USA Medical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute Core Research Institute(CRI)Kyung Hee UniversitySeoul 02447Republic of Korea Center for Brain Technology Brain Science Institute

Polystyrene nanoparticles pose significant toxicological risks to aquatic ecosystems,yet their impact on zebrafish(Danio rerio)embryonic development,particularly erythropoiesis,remains *** study used single-cell RNA sequencing to comprehensively evaluate the effects of polystyrene nanoparticle exposure on erythropoiesis in zebrafish *** vivo validation experiments corroborated the transcriptomic findings,revealing that polystyrene nanoparticle exposure disrupted erythrocyte differentiation,as evidenced by the decrease in mature erythrocytes and concomitant increase in immature ***,impaired heme synthesis further contributed to the diminished erythrocyte *** findings underscore the toxic effects of polystyrene nanoparticles on hematopoietic processes,highlighting their potential to compromise organismal health in aquatic environments.

关键词： Polystyrene nanoparticles Zebrafish embryos Single-cell RNA sequencing Erythropoiesis

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