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2012 MRS Fall Meeting

作者： Wang, Qi Webster, Thomas J. Bioengineering Program College of Engineering Northeastern University Boston MA 02115 United States Department of Chemical Engineering College of Engineering Northeastern University Boston MA 02115 United States

ISBN: (纸本)9781605114750

Bacterial infections are commonly found on paper towels and other paper products leading to the potential spread of bacteria and consequent health concerns. The objective of this in vitro study was to introduce antibacterial properties to paper towel surfaces by coating them with selenium nanoparticles. Scanning electron microscopy was used to measure the size and distribution of the selenium coatings on the paper towels. Atomic force microscopy was used to measure the surface roughness of paper towels before and after coated with selenium nanoparticles. The amount of selenium precipitated on the paper towels was measured by atomic absorption spectroscopy. In vitro bacterial studies with Staphylococcus aureus were conducted to assess the effectiveness of the selenium coating at inhibiting bacterial growth. Results showed that the selenium nanoparticles coated on the paper towel surface were well distributed and semispherical about 50nm in diameter. Most importantly, the selenium nanoparticle coated paper towels inhibited S. aureus growth by 90% after 24 hours and 72 hours compared with the uncoated paper towels. Thus, the study showed that nano-selenium coated paper towels may lead to an increased eradication of bacteria to more effectively clean a wide-range of clinical environments, thus, improving health. © 2013 Materials Research Society.

关键词： Bacteria

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Reduced Adhesion of Staphylococcus aureus to ZnO/PVC Nanocomposites

Reduced Adhesion of Staphylococcus aureus to ZnO/PVC Nanocom...

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Annual Northeast bioengineering Conference

作者： Benjamin M. Geilich Thomas J. Webster Program in Bioengineering and Department of Chemical Engineering College of Engineering Northeastern University Boston MA 02115 USA

ISBN: (纸本)9781467349284

In hospitals and clinics worldwide, medical device surfaces have become a rapidly growing source of nosocomial infections. In particular, patients requiring mechanical ventilation (and, thus, intubation with an endotracheal tube) for extended lengths of time are faced with a high probability of contracting ventilator-associated pneumonia. In this study, polyvinyl chloride (PVC) taken from a conventional endotracheal tube was embedded with varying concentrations of zinc oxide (ZnO) nanoparticles. Staphylococcus aureus biofilms were then grown on these nanocomposite surfaces during a 24-hour culture. Bacterial proliferation on the samples embedded with the highest concentration of ZnO nanoparticles was 87% less when compared to the control, indicating that this technique is effective at reducing biofilm formation on PVC surfaces without the use of antibiotics.

关键词： nanomaterials endotracheal tube biofilm zinc oxide nanoparticles Staphylococcus aureus

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Bacterial adhesion to nanomodified surfaces: Dynamic flow effects on S. aureus and P. aeruginosa

Bacterial adhesion to nanomodified surfaces: Dynamic flow ef...

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2012 MRS Fall Meeting

作者： Machado, Mary C. Tarquinio, Keiko M. Webster, Thomas J. School of Engineering Brown University Providence RI 02912 United States Pediatric Critical Care Medicine Rhode Island Hospital Providence RI 02917 United States Program in Bioengineering Department of Chemical Engineering Boston MA 02115 United States

ISBN: (纸本)9781605114750

Ventilator associated pneumonia (VAP) is a serious and costly clinical problem. Specifically, receiving mechanical ventilation over 24 hours increases the risk of VAP and is associated with high morbidity, mortality and medical costs. Cost effective endotracheal tubes (ETTs) that are resistant to bacterial infection would help to prevent this problem. The objective of this study was to determine differences in bacterial growth on nanomodified and unmodified ETTs under dynamic airway conditions. A bench top model based upon the general design of Hartmann et al. (1999) was constructed to test of the effectiveness of nanomodified ETTs under the airflow conditions present in the airway. Twenty-four hour studies performed in a dynamic flow chamber showed a marked difference in the biofilm formation on different areas of unmodified tubes. Areas where tubes were curved, such as at the entrance to the mouth and the connection between the oropharynx and the larynx, seemed to collect the largest amount of biofilm. The biofilm formation on ETTs in the airflow system after 24 hours showed a large difference depending upon where tubes were oriented within the apparatus. This illustrates the importance of dynamic flow on biofilm formation in pediatric ETTs. It is of particular interest that increased biofilm density on both unmodified and nanomodified tubes appeared to occur at curves in the tube where changes in flow pattern occurred. This emphasizes the need for more accurate models of airflow within pediatric ETTs, suggesting that not only does flow affect pressure gradients along the tube, but in fact, determines the composition of the film itself. More testing is needed to determine the effects of biofilm formation on the efficiency of ETT under airflow, however this study provides significant evidence for nanomodification alone (without the use of antibiotics) to decrease bacteria function. © 2013 Materials Research Society.

关键词： Biofilms

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Introducing Antibacterial Properties to Paper Towels Through the Use of Selenium Nanoparticles

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MRS Online Proceedings Library 2013年第1期1498卷 21-26页

作者： Qi Wang J. Webster Thomas Bioengineering Program and Engineering College of Engineering Northeastern University Boston U.S.A. Department of Chemical Engineering College of Engineering Northeastern University Boston U.S.A.

关键词： coating nanostructure Se

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Proceedings of the Frontiers of Retrovirology Conference 2016

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Retrovirology 2016年第1期13卷 1-40页

作者： Irena Zurnic Sylvia Hütter Nicole Stanke Tobias Kern Fabian Lindel Gesche Gerresheim Martin Hamann Erik Müllers Dirk Lindemann Ute Lehmann Juliane Reh Paul Lesbats Peter Cherepanov Melanie Mann Erik Serrao Alan Engelman Claire Da Silva Santos Kevin Tartour Andrea Cimarelli Rya Burdick Jianbo Chen Jaya Sastri Wei-Shau Hu Vinay Pathak Oliver T. Keppler Patrícia de Sousa-Pereira Hanna-Mari Baldauf Marcel Stern Karine Pradeau Sylvia Eiler Nicolas Levy Marc Ruff Sarah Lennon Sarah Cianferani Stéphane Emiliani Vincent Parissi Sylvie Rato Miguel Munoz Angela Ciuffi Antonio Rausell Amalio Telenti Alexander Zhyvoloup Ian Anderson Chen- Hsuin Lee Ariberto Fassati Anat Melamed Delphine Planas Petronela Ancuta Yuwei Zhang Huicheng Chen Annie Bernier Annie Gosselin Janos Kriston-Vizi Robin Ketteler Andy Merritt Charles Bangham Jocelyn Turpin Anthony Rodari Benoit Van Driessche Mathilde Galais Nadége Delacourt Sylvain Fauquenoy Caroline Vanhulle Anna Kula Carine Van Lint Ernst J. Wolvetang Arsène Burny Olivier Rohr Thijs van Montfort Renee van der Sluis Ben Berkhout Dave Speijer Gang Wang Na Zhao Atze T. Das Mirte Scherpenisse Bo Meng Andrzej Rutkowski Lars Dölken Andrew Lever Neil Berry Claire Ham Neil Almond Hoi Ping Mok Nicholas Norton Axel Fun Jack Hirst Mark Wills Chris Hellmund Harriet Groom Thomas Schuster Benedikt Asbach Ralf Wagner Christine Gross Martina Kalmer Andrea K. Thoma-Kress Norbert Donhauser Sebastian Millen Sandra M. Müller-Schmucker Veit Wiesmann Thomas Wittenberg Jan Gettemans Minghua Li Shan-Lu Liu Eric O. Freed Janis Müller Jan Münch Simone Joas Clement Wesley Gnanadurai Edina Lump Christina M. Stürzel Daniel Sauter Frank Kirchhoff Katharina Mack Kathrin Starz Christina Stürzel Xaver Sewald Nasim Motamedi Pradeep Uchil Ruoxi Pi Christin Herrmann Walther Mothes Mark Ladinsky Pamela Bjorkman Jagadish Beloor Priti Kumar Thomas Murooka Thorsten Mempel Michael Brehm Dale Greiner Erica Parrish Nicholas F. Parrish Beatrice H. Hahn Frederic Bibollet-Ruche Ulrike Sauermann Katharina Töpfer Tina Schultheiss Chris Molecular Virology and Gene Therapy Molecular Medicine Leuven Belgium Molecular Virology Technical University Dresden Dresden Germany Center for Regenerative Therapies Dresden Dresden Germany Potsdam University Potsdam Germany The Francis Crick Institute London Great Britain Institute of Clinical and Molecular Virology Friedrich-Alexander-Universität Erlangen-Nuremberg Erlangen Germany Dana Farber Cancer Institute Boston MA United States CIRI Lyon France National Cancer Institute Frederick MD United States Virology Max von Pettenkofer-Institut LMU Munich Munich Germany Institute of Medical Virology University of Frankfurt Frankfurt a. M. Germany German Center for Infection Research Munich Germany Institute of Medical Virology University Hospital Frankfurt Frankfurt a. M. Germany CIBIO/InBIO University of Porto Vairão Portugal Faculty of Sciences University of Porto Porto Portugal Virology Max von Pettenkofer Institute LMU Munich Germany Institute for Medical Virology Frankfurt a. M. Germany Integrative structural Biology IGBMC Illkirch France Institut Pluridisciplinaire Hubert Curien Strasbourg France Institut Cochin Paris France CNRS UMR5234 MFP Lab Bordeaux France Associated International Laboratory (LIA) Microbiology and Immunology CNRS/Université de Bordeaux/Heinrich Pette Institute-Leibniz Institute for Experimental Virology Bordeaux/Hamburg France Institute of Microbiology University Hospital Center and University of Lausanne Lausanne Switzerland Imagine Institute Paris Descartes University Paris France J. Craig Venter Institute La Jolla CA United States Infection University College London London Great Britain Medicine Imperial College London Great Britain Microbiology & Infection University of Montreal Montreal Great Britain CRCHUM Montreal Canada University College London LMCB London Great Britain MRC Technology Centre for Therapeutic Discovery London Great Britain Section of Virology Department of Medicine Imperial Co

Oral presentationsSession 1: Entry & uncoatingO1 Host cell polo-like kinases (PLKs) promote early prototype foamy virus (PFV) replicationIrena Zurnic, Sylvia Hütter, Ute Lehmann, Nicole Stanke, Juliane Re... 详细信息

Oral presentations

Session 1: Entry & uncoating

O1 Host cell polo-like kinases (PLKs) promote early prototype foamy virus (PFV) replication

Irena Zurnic, Sylvia Hütter, Ute Lehmann, Nicole Stanke, Juliane Reh, Tobias Kern, Fabian Lindel, Gesche Gerresheim, Martin Hamann, Erik Müllers, Paul Lesbats, Peter Cherepanov, Erik Serrao, Alan Engelman, Dirk Lindemann

O2 A novel entry/uncoating assay reveals the presence of at least two species of viral capsids during synchronized HIV-1 infection

Claire Da Silva Santos, Kevin Tartour, Andrea Cimarelli

O3 Dynamics of nuclear envelope association and nuclear import of HIV-1 complexes

Rya Burdick, Jianbo Chen, Jaya Sastri, Wei-Shau Hu, Vinay Pathak

O4 Human papillomavirus protein E4 potently enhances the susceptibility to HIV infection

Oliver T. Keppler

Session 2: Reverse transcription & integration

O5 Structure and function of HIV-1 integrase post translational modifications

Karine Pradeau, Sylvia Eiler, Nicolas Levy, Sarah Lennon, Sarah Cianferani, Stéphane Emiliani, Marc Ruff

O6 Regulation of retroviral integration by RNA polymerase II associated factors and chromatin structure

Vincent Parissi

Session 3: Transcription and latency

O7 A novel single-cell analysis pipeline to identify specific biomarkers of HIV permissiveness

Sylvie Rato, Antonio Rausell, Miguel Munoz, Amalio Telenti, Angela Ciuffi

O8 A capsid-dependent integration program linking T cell activation to HIV-1 gene expression

Alexander Zhyvoloup, Anat Melamed, Ian Anderson, Delphine Planas, Janos Kriston-Vizi, Robin Ketteler, Chen-Hsuin Lee, Andy Merritt, Petronela Ancuta, Charles Bangham, Ariberto Fassati

O9 Characterisation of new RNA polymerase III and RNA polymerase II transcriptional promoters in the Bovine Leukemia Virus genome

Anthony Rodari, Benoit Van Driessche, Mathilde Galais, Nadége Delacourt, Sylv

关键词： Human Immunodeficiency Virus Infection Simian Immunodeficiency Virus Feline Immunodeficiency Virus Bovine Leukemia Virus Prototype Foamy Virus

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Bacterial Adhesion to Nanomodified Surfaces: Dynamic Flow Effects on S. aureus and P. aeruginosa

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MRS Online Proceedings Library 2013年第1期1498卷 79-84页

作者： C. Machado Mary M. Tarquinio Keiko J. Webster Thomas School of Engineering Brown University Providence USA Pediatric Critical Care Medicine Rhode Island Hospital Providence USA Program in Bioengineering and Department of Chemical Engineering Boston USA

关键词： nanoscale biomedical fluid

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Single cell western blotting

Single cell western blotting

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17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

作者： Hughes, Alex J. Spelke, Dawn P. Xu, Zhuchen Schaffer, David V. Herr, Amy E. Department of Bioengineering University of California UC Berkeley Berkeley CA United States UC Berkeley-UCSF Graduate Program in Bioengineering UC Berkeley Berkeley CA United States Department of Chemical Engineering UC Berkeley Berkeley CA United States Helen Wills Neuroscience Institute UC Berkeley Berkeley CA United States California Institute for Quantitative Biosciences UC Berkeley Berkeley CA United States

Understanding protein-mediated cell signaling and differentiation processes requires capturing response heterogeneity for single cells in large populations. We report single-cell western blots on standard microscope s... 详细信息

ISBN: (纸本)9781632666246

关键词： Proteins

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engineering Microorganisms for Biomedical and Energy Applications

Engineering Microorganisms for Biomedical and Energy Applica...

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第八届全国微全分析系统学术会议、第三届全国微纳尺度生物分离分析学术会议暨第五届国际微化学与微系统学术会议

作者： I-Ming Hsing Danhui Cheng Department of Chemical and Biomolecular Engineering the Hong Kong University of Science and TechnologyHong Kong SARChina Division of Biomedical Engineering and Bioengineering Graduate Programthe Hong Kong University of Science and Technology Division of Biomedical Engineering and Bioengineering Graduate Program the Hong Kong University of Science and Technology

The availability of technologies to rapidly synthesize and decode genetic sequences has provided new applications for recombinant DNA technology and genetic engineering developed in early *** new genetic engineering approach,also coined as synthetic biology by many scientists,often involves engineering of microorganism through expression of heterologous gene segments in the microbial *** ability to engineer microorganisms empowered with tailor-made characteristics has provided exciting opportunities in different areas of *** instance,drug synthesis and novel drug delivery methods based on engineered bacteria strains have been extensively studied in biomedical ***,various synthetic biology approaches have been proposed to construct novel metabolic pathways in order to achieve higher yield in bioenergy & biofuel production,such as in ethanol fermentation,hydrogen production and bioelectricity generation through a microbial fuel *** this plenary presentation,we will start with an overview of this very exciting technology (*** biology),followed by a detailed discussion of two related projects in my *** the first part,we utilized yeast display technology for developing a new cell-based immunoassay *** the second one,we engineered photosynthetic bacteria to rewire its indigenous electron transfer pathway in pursuit of converting solar energy to ***,for the interests of our microfluidic community,some thoughts and opportunities of studying and utilizing engineered microorganisms in a microfluidic platform will be discussed.

关键词： Synthetic Biology Genetic engineering Yeast Surface Display Photosynthetic Bacteria

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Conference Scene: Scientists discuss the future of nanomedicine

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Nanomedicine 2013年第11期8卷 1747-1749页

作者： Benjamin M Geilich Thomas J Webster Program in Bioengineering & Department of Chemical Engineering Northeastern University Boston MA 02115 USA Center of Excellence for Advanced Materials Research King Abdulaziz University Jeddah 21589 Saudi Arabia.

The First International Translational Nanomedicine Conference was recently held at Northeastern University (MA, USA) from 26 to 28 July 2013, in the heart of historic Boston (MA, USA). Sponsored by the International Journal of Nanomedicine and Northeastern University’s College of engineering, the conference focused on strategies for turning the advances in nanomedicine research into actual products, as well as addressing other issues pertinent to the future of the field. Plenary talks were given by distinguished Northeastern University faculty members for 1 h, and 30-min technical talks were given by experts from all over the world. Interspersed through the lectures, over 100 students and researchers presented posters detailing their latest findings.

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engineering Rhodobacter Sphaeroides for direct electron transfer by heterogenous expression of redoxactive components from electrogenic species

Engineering Rhodobacter Sphaeroides for direct electron tran...

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Food, Pharmaceutical and bioengineering Division 2013 - Core programming Area at the 2013 AIChE Annual Meeting: Global Challenges for engineering a Sustainable Future

作者： Cheng, Danhui Wang, Wanyang Chow, King L. Hsing, I.-Ming Bioengineering Graduate Program Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong Division of Biomedical Engineering Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong Department of Chemical and Biomolecular Engineering Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong Division of Life Science Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong

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