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arXiv 2022年

作者： Schwartz, Jonathan Harris, Chris Pietryga, Jacob Zheng, Huihuo Kumar, Prashant Visheratina, Anastasiia Kotov, Nicholas A. Major, Brianna Avery, Patrick Ercius, Peter Ayachit, Utkarsh Geveci, Berk Muller, David A. Genova, Alessandro Jiang, Yi Hanwell, Marcus Hovden, Robert Department of Material Science and Engineering University of Michigan Ann ArborMI United States Kitware Inc Clifton ParkNY United States Argonne Leadership Computing Facility Argonne National Laboratory LemontIL United States Department of Chemical Engineering University of Michigan Ann ArborMI United States The Molecular Foundry Lawrence Berkeley National Laboratory BerkeleyCA United States School of Applied and Engineering Physics Cornell University IthacaNY United States Advanced Photon Source Argonne National Laboratory LemontIL United States National Synchrotron Light Source II Brookhaven National Laboratory UptonNY United States Applied Physics Program University of Michigan Ann ArborMI United States

The demand for high-throughput electron tomography is rapidly increasing in biological and material sciences. However, this 3D imaging technique is computationally bottlenecked by alignment and reconstruction which runs from hours to days. We demonstrate real-time tomography with dynamic 3D tomographic visualization to enable rapid interpretation of specimen structure immediately as data is collected on an electron microscope. Using geometrically complex chiral nanoparticles, we show volumetric interpretation can begin in less than 10minutes and a high-quality tomogram is available within 30 minutes. Real-time tomography is integrated into tomviz, an opensource and cross-platform 3D data analysis tool that contains intuitive graphical user interfaces (GUI), to enable any scientist to characterize biological and material structure in 3D. © 2022, CC BY.

关键词： Graphical user interfaces

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Plasma-Derived Nanoclusters for Site-Specific Multimodality Photo/Magnetic Thrombus Theranostics

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Advanced Healthcare Materials 2023年第28期12卷 e2301504页

作者： Liu, Chia-Hung Liu, Ming-Che Jheng, Pei-Ru Yu, Jiashing Fan, Yu-Jui Liang, Jia-Wei Hsiao, Yu-Cheng Chiang, Chih-Wei Bolouki, Nima Lee, Jyh-Wei Hsieh, Jang-Hsing Mansel, Bradley W Chen, Yan-Ting Nguyen, Hieu Trung Chuang, Er-Yuan Department of Urology School of Medicine College of Medicine Taipei Medical University 250 Wu-Hsing Street Taipei11031 Taiwan TMU Research Center of Urology and Kidney Taipei Medical University 250 Wu-Hsing Street Taipei11031 Taiwan Department of Urology Shuang Ho Hospital Taipei Medical University 291 Zhongzheng Road Zhonghe District New Taipei City23561 Taiwan Clinical Research Center Taipei Medical University Hospital Taipei11031 Taiwan School of Dental Technology College of Oral Medicine Taipei Medical University Taipei11031 Taiwan Graduate Institute of Biomedical Materials and Tissue Engineering International Ph.D. Program in Biomedical Engineering Graduate Institute of Biomedical Optomechatronics School of Biomedical Engineering Research Center of Biomedical Device Innovation Entrepreneurship Education Center College of Interdisciplinary Studies Taipei Medical University Taipei11031 Taiwan Department of Chemical Engineering College of Engineering National Taiwan University Taipei106 Taiwan Department of Orthopedics Taipei Medical University Taipei11031 Taiwan Department of Orthopedics Taipei Medical University Hospital Taipei11031 Taiwan Department of Physical Electronics Faculty of Science Masaryk University Brno60177 Czech Republic Department of Materials Engineering Ming Chi University of Technology New Taipei City24301 Taiwan Center for Plasma and Thin Film Technologies Ming Chi University of Technology New Taipei City24301 Taiwan National Synchrotron Radiation Research Center Hsinchu Science Park Hsinchu30076 Taiwan Department of Orthopedics and Trauma Faculty of Medicine University of Medicine and Pharmacy at Ho Chi Minh City Ho Chi Minh City700000 Viet Nam Cell Physiology and Molecular Image Research Center Taipei Medical University Wan Fang Hospital Taipei11696 Taiwan Precision Medicine and Translational Cancer Research Center Taipei Medical University Hospital Taipei11031 Taiwan

Traditional thrombolytic therapeutics for vascular blockage are affected by their limited penetration into thrombi, associated off-target side effects, and low bioavailability, leading to insufficient thrombolytic efficacy. It is hypothesized that these limitations can be overcome by the precisely controlled and targeted delivery of thrombolytic therapeutics. A theranostic platform is developed that is biocompatible, fluorescent, magnetic, and well-characterized, with multiple targeting modes. This multimodal theranostic system can be remotely visualized and magnetically guided toward thrombi, noninvasively irradiated by near-infrared (NIR) phototherapies, and remotely activated by actuated magnets for additional mechanical therapy. Magnetic guidance can also improve the penetration of nanomedicines into thrombi. In a mouse model of thrombosis, the thrombosis residues are reduced by ≈80% and with no risk of side effects or of secondary embolization. This strategy not only enables the progression of thrombolysis but also accelerates the lysis rate, thereby facilitating its prospective use in time-critical thrombolytic treatment. © 2023 Wiley-VCH GmbH.

关键词： Diseases

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Recent Advancements in engineering Strategies for Manipulating Neural Stem Cell Behavior

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Current tissue microenvironment reports 2020年第2期1卷 41-47页

作者： Brian J O'Grady Ethan S Lippmann Department of Chemical and Biomolecular Engineering Vanderbilt University Nashville TN USA. Department of Biomedical Engineering Vanderbilt University Nashville TN USA. Vanderbilt Brain Institute Vanderbilt University Nashville TN USA. Interdisciplinary Materials Science Program Vanderbilt University Nashville TN USA.

PURPOSE OF REVIEW:Stem cells are exquisitely sensitive to biophysical and biochemical cues within the native microenvironment. This review focuses on emerging strategies to manipulate neural cell behavior using these influences in three-dimensional (3D) culture systems. RECENT FINDINGS:Traditional systems for neural cell differentiation typically produce heterogeneous populations with limited diversity rather than the complex, organized tissue structures observed . Advancements in developing engineering tools to direct neural cell fates can enable new applications in basic research, disease modeling, and regenerative medicine. SUMMARY:This review article highlights engineering strategies that facilitate controlled presentation of biophysical and biochemical cues to guide differentiation and impart desired phenotypes on neural cell populations. Specific highlighted examples include engineered biomaterials and microfluidic platforms for spatiotemporal control over the presentation of morphogen gradients.

关键词： biomaterial morphogen gradient neural stem cell stem cell differentiation

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The potential and prospect of biomass as primary energy in Indonesia

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IOP Conference Series: Earth and Environmental science 2021年第1期896卷

作者： A I D Lantasi Syafrudin Budiyono Master program of Environmental Science School of Postgraduate Studies Diponegoro University Department of Environmental Engineering Faculty of Engineering Diponegoro University Department of Chemical Engineering Faculty of Engineering Diponegoro University

Energy is one of the needs to support daily human activities. Without energy, various activities such as industry, health, and household will be disrupted. Total energy consumption in Indonesia in 2018 reached 875 million barrels of oil equivalent (BOE), which was dominated by fuel oil. It illustrates that national energy consumption is still dominated by primary energy sources originating from fossil fuels. Various previous studies have suggested the environmental and health impacts of air pollution from burning fossil fuels. This article will briefly discuss biomass as a primary energy source based on the results of literature studies. The discussion section will discuss the environmental impacts of biomass utilization based on several scientific studies and the form of biomass utilization in Indonesia that has been carried out. As a follow-up to several scientific facts, it is necessary to mix other primary energy from new and renewable energy sources.

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Novel Biosynthesis of Spherical Tungsten Trioxide Nanoparticles for Sustainable Photocatalytic Application

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Applied Organometallic Chemistry 2025年第6期39卷

作者： Nazish Parveen Kholoud M. Alnahdi Shada A. Alsharif Sajid Ali Ansari Mohd Zahid Ansari Ahmad Umar Chawki Awada Haya Abdullah Al-Dosari Department of Chemistry College of Science King Faisal University Al-Hasa Saudi Arabia Saudi Arabia Department of Physics Faculty of Science University of Tabuk Tabuk Saudi Arabia University College of Umluj University of Tabuk Tabuk Saudi Arabia Department of Physics College of Science King Faisal University Hofuf Al-Ahsa Saudi Arabia Chemical Engineering Program Texas A&M University at Qatar Doha Qatar Department of Chemistry College of Science and Arts and Promising Centre for Sensors and Electronic Devices (PCSED) Najran University Najran Saudi Arabia STEM Pioneers Training Lab Najran University Najran Saudi Arabia Department of Materials Science and Engineering The Ohio State University Columbus Ohio USA

We developed a novel method for synthesizing tungsten trioxide nanoparticles, utilizing orange juice as a sustainable and economical catalyst. This innovative approach eliminates the need for expensive metals and harsh chemicals. The natural components of orange juice, particularly ascorbic acid and citric acid, play a crucial role in the formation and stabilization of these nanoparticles. We thoroughly tested the synthesized nanoparticles using a suite of analytical techniques, including X-ray diffraction, X-ray photoelectron spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and Fourier transform infrared spectroscopy. The results were compelling. When exposed to UV light, the nanoparticles were able to degrade approximately 95% of the model pollutant, methyl orange, demonstrating a rapid and efficient performance. Without the presence of these nanoparticles, the degradation of the pollutant was minimal. This confirms the effectiveness of the tungsten trioxide nanoparticles as a potent and environmentally friendly solution for pollutant degradation in various applications. This method not only offers a green alternative to traditional nanoparticle synthesis but also highlights the potential of using common natural resources in advanced environmental applications.

关键词： biosynthesis photocatalytic activity sustainable materials UV light WO3 NPs

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Comparative toxicity of silver nanoparticles and silver ions to Escherichia coli

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Journal of Environmental sciences 2018年第4期30卷 50-60页

作者： Yoojin Choi Hyun-A Kim Kyoung-Woong Kim Byung-Tae Lee Department of Chemical and Biomolecular Engineering (BK21 Plus Program) Korea Advanced Institute of Science and Technology (KAIST) School of Earth Sciences and Environmental Engineering Gwangju Institute of Science and Technology (GIST)

With the increase in silver（Ag）-based products in our lives, it is essential to test the potential toxicity of silver nanoparticles（Ag NPs） and silver ions（Ag ions） on living organisms under various conditions. Here, we investigated the toxicity of Ag NPs with Ag ions to Escherichia coli K-12 strain under various conditions. We observed that both Ag NPs and Ag ions display antibacterial activities, and that Ag ions had higher toxicity to E. coli K-12 strain than Ag NPs under the same concentrations. To understand the toxicity of Ag NPs at a cellular level, reactive oxygen species（ROS） enzymes were detected for use as antioxidant enzymatic biomarkers. We have also studied the toxicity of Ag NPs and Ag ions under various coexistence conditions including： fixed total concentration, with a varied the ratio of Ag NPs to Ag ions; fixed the Ag NPs concentration and then increased the Ag ions concentration; fixed Ag ions concentration and then increasing the Ag NPs *** to Ag NPs and Ag ions clearly had synergistic toxicity; however, decreased toxicity（for a fixed Ag NPs concentration of 5 mg/L, after increasing the Ag ions concentration） to E. coli K-12 strain. Ag NPs and Ag ions in the presence of L-cysteine accelerated the bacterial cell growth rate, thereby reducing the bioavailability of Ag ions released from Ag NPs under the single and coexistence conditions. Further works are needed to consider this potential for Ag NPs and Ag ions toxicity across a range of environmental *** Significance Statement： As silver nanoparticles（Ag NPs）-based products are being broadly used in commercial industries, an ecotoxicological understanding of the Ag NPs being released into the environment should be further considered. Here, we investigate the comparative toxicity of Ag NPs and silver ions（Ag ions） to Escherichia coli K-12 strain, a representative ecotoxicological bioreporter. This study showed that toxicities of Ag NPs and Ag ions to E. coli

关键词： Nanotoxicity Silver nanoparticles Silver ions Coexist condition Escherichia coli

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Phage therapy

NATURE REVIEWS METHODS PRIMERS

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NATURE REVIEWS METHODS PRIMERS 2025年第1期5卷 1页

作者： [Anonymous] Human Microbiome Research Program Department of Bacteriology and Immunology Faculty of Medicine University of Helsinki Helsinki Finland European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Study Group for Non-traditional Antibacterial Therapy (ESGNTA) Basel Switzerland Institute of Biomedical and Oral Research (IBOR) Faculty of Dental Medicine Hebrew University of Jerusaleum Jerusalem Israel The Israeli Phage Center (IPTC) of the Hebrew University and Hadassah Medical Center Jerusalem Israel Department of Clinical Microbiology and Infectious Diseases Hadassah–Hebrew University Medical Center Jerusalem Jerusalem Israel Laboratory of Gene Technology Department of Biosystems KU Leuven Leuven Belgium Leicester Centre for Phage Research Department of Genetics and Genome Biology University of Leicester Leicester UK Center for Evolutionary Hologenomics Globe Institute University of Copenhagen Copenhagen Denmark Centre of Excellence for Omics-Driven Computational Biodiscovery (COMBio) AIMST University Bedong Kedah Malaysia Laboratory of Phage Molecular Biology Institute of Immunology and Experimental Therapy Wrocław Poland Wrocław University of Science and Technology Faculty of Medicine Wrocław Poland Department of Biological Sciences University of Pittsburgh Pittsburgh PA USA Laboratory for Molecular and Cellular Technology Queen Astrid Military Hospital Brussels Belgium Department of Biological and Environmental Science Nanoscience Center University of Jyväskylä Jyväskylä Finland Chemical Engineering Department Loughborough University Loughborough UK

Bacteriophage (phages) are viruses that exclusively use bacterial cells for propagation, killing the bacterial host in the process. In phage therapy, phages are used to reduce bacterial numbers, thereby curing bacterial infections. Although this principle is conceptually straightforward, its practical application faces several hurdles. In this Primer, the practical aspects of phage therapy are outlined. We introduce the microbiological methods used to prepare and characterize phages and elucidate their interactions with bacteria. The discussion covers how the information in complete phage genome sequences is used, along with how RNA sequencing can enhance our understanding of phage biology. Selection parameters for therapeutic phages for clinical applications and key elements in industrial-scale phage production are provided. A summary of clinical trials both past and present, phage administration and dosing issues is analysed, as well as limitations associated with phage therapy and mitigation strategies. Finally, we speculate on the future of phage therapy.

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Phytocannabinoids: Pharmacological effects, biomedical applications, and worldwide prospection

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Journal of traditional and complementary medicine 2023年第6期13卷 575-587页

作者： Ana L G de Brito Siqueira Pedro V V Cremasco Juliana O Bahú Aline Pioli da Silva Lucas R Melo de Andrade Paula G A González Sara Crivellin Viktor O Cárdenas Concha Karolline Krambeck Leandro Lodi Patrícia Severino Eliana B Souto Institute of Science and Technology Federal University of Alfenas (UNIFAL) Poços de Caldas 37715-400 Minas Gerais Brazil. National Institute of Science and Technology in Biofabrication (INCT-BIOFABRIS) School of Chemical Engineering University of Campinas Albert Einstein Ave. Cidade Universitária Zeferino Vaz Campinas 13083-852 SP Brazil. Institute of Environmental Chemical and Pharmaceutical Science School of Chemical Engineering Federal University of São Paulo (UNIFESP) São Nicolau St. Jd. Pitangueiras Diadema 09913-030 SP Brazil. Laboratory of Pharmaceutical Technology Faculty of Pharmaceutical Sciences Food and Nutrition Federal University of Mato Grosso do Sul Campo Grande 79070-900 MS Brazil. Laboratory of Pharmaceutical Technology Department of Drug Sciences Faculty of Pharmacy University of Porto 4050-313 Porto Portugal. UCIBIO - Applied Molecular Biosciences Unit MEDTECH Faculty of Pharmacy University of Porto 4050-313 Porto Portugal. Associate Laboratory i4HB - Institute for Health and Bioeconomy Faculty of Pharmacy University of Porto 4050-313 Porto Portugal. Laboratory of Nanotechnology and Nanomedicine (LNMed) Institute of Technology and Research (ITP) Murilo Dantas Ave. 300 Aracaju 49010-390 Sergipe Brazil. Industrial Biotechnology Program University of Tiradentes (UNIT) Murilo Dantas Ave. 300 Aracaju 49010-390 Sergipe Brazil.

Scientific evidence exists about the association between neurological diseases (i.e., Parkinson's disease, Alzheimer's disease, amyotrophic lateral sclerosis (ALS), multiple sclerosis, depression, and memory loss) and oxidative damage. The increasing worldwide incidence of such diseases is attracting the attention of researchers to find palliative medications to reduce the symptoms and promote quality of life, in particular, in developing countries, e.g., South America and Africa. Among potential alternatives, extracts of L. are suitable for people who have neurological disorders, spasticity, and pain, nausea, resulting from diseases such as cancer and arthritis. In this review, we discuss the latest developments in the use of Cannabis, its subtypes and constituents, extraction methods, and relevant pharmacological effects. Biomedical applications, marketed products, and prospects for the worldwide use of L. extracts are also discussed, providing the bibliometric maps of scientific literature published in representative countries from South America (i.e., Brazil) and Africa (i.e., South Africa). A lack of evidence on the effectiveness and safety of Cannabis, besides the concerns about addiction and other adverse events, has led many countries to act with caution before changing Cannabis-related regulations. Recent findings are expected to increase the social acceptance of Cannabis, while new technologies seem to boost the global cannabis market because the benefits of (-)-trans-delta-9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD) use have been proven in several studies in addition to the potential to general new employment.

关键词： Cannabidiol Cannabis Sativa L. Commercial products Market Trans-Delta-9-tetrahydrocannabinol

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Tribological properties of hydroxyapatite-coated nanorods on Ti-6Al-4V surfaces

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Scientific reports 2025年第1期15卷 19113页

作者： Sarah Akua Osafo Tabiri Asumadu Desmond Klenam Precious Etinosa John David Obayemi Benjamin Agyei-Tuffour Abu Yaya David Dodoo-Arhin Stanley Chijioke Eluu Wole Soboyejo Department of Materials Science and Engineering School of Engineering Sciences College of Basic and Applied Sciences University of Ghana Legon Accra Ghana. Department of Biomaterial Sciences Dental School University of Ghana Korle Bu Campus Accra Ghana. Department of Mechanical Engineering Program in Materials Science and Engineering Worcester Polytechnic Institute 100 Institute Road Worcester MA 01609 USA. Department of Materials Engineering Sunyani Technical University Sunyani Ghana. Global Center of Advanced Materials and Manufacturing Department of Mechanical Engineering College of Engineering State University of New York (SUNY) Polytechnic Institute 100 Seymour Street Utica NY 13502 USA. Next Frontiers in Advanced Material Laboratory School of Chemical and Metallurgical Engineering University of the Witwatersrand 1 Jan Smuts Avenue Johannesburg 2050 South Africa. Department of Biomedical Engineering Worcester Polytechnic Institute Gateway Park Life Sciences and Bioengineering Centre 60 Prescott Street Worcester MA 01609 USA. Department of Biotechnology Ebonyi State University Abakaliki Nigeria. Department of Pharmaceutical Microbiology and Biotechnology Faculty of Pharmaceutical Sciences Nnamdi Azikiwe University Awka Anambra State Nigeria. Department of Mechanical Engineering Program in Materials Science and Engineering Worcester Polytechnic Institute 100 Institute Road Worcester MA 01609 USA. soboyew@sunypoly.edu. Global Center of Advanced Materials and Manufacturing Department of Mechanical Engineering College of Engineering State University of New York (SUNY) Polytechnic Institute 100 Seymour Street Utica NY 13502 USA. soboyew@sunypoly.edu. Department of Biomedical Engineering Worcester Polytechnic Institute Gateway Park Life Sciences and Bioengineering Centre 60 Prescott Street Worcester MA 01609 USA. soboyew@sunypoly.edu.

This paper presents the tribological properties and bioactivity of nanostructured hydroxyapatite (HA) from biowaste sources and coated onto Ti-6Al-4V substrates using a novel pack cementation method. The process introduced HA pillars/nanorods on the surfaces of Ti-6Al-4V to enhance their osseointegration for dental implants. The mechanical and tribological properties were studied with nanoindentation, and pin-on-disk techniques, following the microstructural characterization of the coatings with atomic force microscopy, X-ray diffraction, Raman spectroscopy, scanning electron microscopy, and energy dispersive x-ray spectroscopy. The study also examines the surface bioactivity and elucidates the underlying friction and wear mechanisms of the HA-coated and annealed Ti-6Al-4V surface. The study results show a bone bonding capacity of the biowaste-derived HA-coated substrate with improved hardness and tribological properties. The implications of the study are discussed for the development of nano-structured HA-coated Ti-6Al-4V for dental implants with improved osseointegration for dental and biomedical applications.

关键词： Bioactivity Dental implants Osseointegration Surface modification Titanium alloys Wear resistance

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Recyclable Fenton-like catalyst based on zeolite Y supported ultrafine,highly-dispersed Fe_2O_3 nanoparticles for removal of organics under mild conditions

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Chinese chemical Letters 2019年第2期30卷 324-330页

作者： Xuanyu Yang Xiaowei Cheng Ahmed A.Elzatahry Jinyang Chen Abdulaziz Alghamdi Yonghui Deng Department of Chemistry State Key Laboratory of Molecular Engineering of Polymers Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials iChEM (Collaborative Innovation Center of Chemistry for Energy Materials) Fudan University Materials Science and Technology Program College of Arts and Sciences Qatar University School of Environmental and Chemical Engineering Shanghai University Department of Chemistry College of Science King Saud University State Key Lab of Transducer Technology Shanghai Institute of Microsystem and Information Technology Chinese Academy of Sciences

A versatile wet impregnation method was employed to conveniently and controllably deposit Fe_2O_3 nanoparticles on zeolites including commercial Y, mordenite and ZSM-5 with the similar framework Si/Al ratios and crystal sizes, respectively. The ultrafine Fe_2O_3 nanoparticles in size of 5 nm can be highly dispersed on zeolite Y matrix due to its much better wettability than ZSM-5 and mordenite. By using the obtained Fe_2O_3/zeolite composite as the heterogeneous Fenton-like catalysts, the degradation of phenol as a model reaction was systematically investigated, including the zeolite supports, particle size and dispersion of Fe_2O_3, and reaction conditions of H_2O_2 concentration, temperature, and pH value. The catalyst based on zeolite Y with Fe loading of 9% exhibited the best phenol degradation efficiency (> 90%)in neutral pH within 2 h. Its high catalytic activity in Fenton reaction can be attributed to the bifunctional properties of strong surface BrФnsted acidity and high reactivity of octahedral Fe^(3+) in the highlydispersed ultrafine Fe_2O_3 nanoparticles in size of 5 nm, which were the primary active centers to quickly decompose H_2O_2 into hydroxyl radicals. Since phenol degradation can be performed under mild conditions of ambient temperature (283-323 K) and a wide pH range (4.0-7.0), the catalysts can be easily recovered for recyclable use with stable degradation activity, which own the immense potential in deep treatment of organic pollutants in industrial wastewater.

关键词： Fenton-like reaction Zeolite Fe2O3 nanoparticles Highly-dispersed Phenol degradation

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