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Optimization of nonspecific protease activity fabrication by Bacillus subtilis N30 isolated from Taiwan using different models of response surface methodology

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Biocatalysis and Agricultural Biotechnology 2023年 50卷

作者： Hong-Quang Luong Thanh-Ninh Le Pei-Hsuan Lee Pao-Chuan Hsieh Department of Food Science National Pingtung University of Science and Technology Pingtung 91207 Taiwan ROC Faculty of Chemical Engineering and Food Technology Nong Lam University Ho Chi Minh City Viet Nam Bachelor of Program in Scientific Agriculture National Pingtung University of Science and Technology Pingtung 91207 Taiwan ROC

This study aimed to identify the best growth conditions for Bacillus subtilis N30 to produce viable cell counts , pH values, and protease activity through fermentation. The research employed response surface criteria and desirability function analysis. Box-Behnken and central composite designs (BBD and CCD) were used to determine the number of processing variables and experimental runs. The results of screening single factor experimental design as maltose content (0.5–1.5% w/v), malt extract concentration (0.25%–0.75% w/v), and incubation time between 30 and 42 h were applied for coded levels (−1, 0, +1) of each independent variable correlated to steps of 0.5, 0.25, and 6, respectively. Viable cell count, pH level, and protease activity were response variables. Analysis of variance (ANOVA) revealed no significant difference when using CCD but significant linear, quadratic, and interaction influences of fermentation parameters on the response of protease activity when using BBD. Both the BBD and CCD models did not fit the viable cell count and pH. According to numerical optimization, the ideal fermentation parameters were a maltose concentration of 1.19%, a malt extract of 0.56%, and a culture time of 33 h, with a predicted protease activity yield of 5.88 U/ml. This optimum condition for growing Bacillus subtilis N30 with high protease activity will be applied in the fermentation of soy milk since it contains many short-chain peptides (2–20 amino acids), which may help prevent and manage high blood pressure for human beings.

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Partially Ion-Paired Solvation Structure Design for Lithium-Sulfur Batteries under Extreme Operating Conditions

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Angewandte Chemie 2023年第5期136卷

作者： Dr. Guorui Cai Hongpeng Gao Mingqian Li Varun Gupta John Holoubek Prof. Tod A. Pascal Prof. Ping Liu Prof. Zheng Chen Department of NanoEngineering University of California San Diego La Jolla CA 92093 USA Program of Materials Science and Engineering University of California San Diego La Jolla CA 92093 USA Sustainable Power and Energy Center University of California San Diego La Jolla CA 92093 USA Program of Chemical Engineering University of California San Diego La Jolla CA 92093 USA

Achieving increased energy density under extreme operating conditions remains a major challenge in rechargeable batteries. Herein, we demonstrate an all-fluorinated ester-based electrolyte comprising partially fluorinated carboxylate and carbonate esters. This electrolyte exhibits temperature-resilient physicochemical properties and moderate ion-paired solvation, leading to a half solvent-separated and half contact-ion pair in a sole electrolyte. As a result, facile desolvation and preferential reduction of anions/fluorinated co-solvents for LiF-dominated interphases are achieved without compromising ionic conductivity (>1 mS cm −1 even at −40 °C). These advantageous features were found to apply to both lithium metal and sulfur-based electrodes even under extreme operating conditions, allowing stable cycling of Li || sulfurized polyacrylonitrile (SPAN) full cells with high SPAN loading (>3.5 mAh cm −2 ) and thin Li anode (50 μm) at −40, 23 and 50 °C. This work offers a promising path for designing temperature-resilient electrolytes to support high energy density Li metal batteries operating in extreme conditions.

关键词： Battery Electrolyte Extreme Temperatures Ion Solvation Ion Transports

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Cascade Synthesis of Fe-N2-Fe Dual-Atom Catalysts for Superior Oxygen Catalysis

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Angewandte Chemie 2024年第40期136卷

作者： Shuang Zhao Minjie Liu Zehua Qu Yan Yan Zhirong Zhang Jifeng Yang Siyuan He Zhou Xu Yiquan Zhu Laihao Luo Kwun Nam Hui Mingkai Liu Jie Zeng School of Chemistry & Materials Science Jiangsu Key Laboratory of Green Synthetic Chemistry for Functional Materials Jiangsu Normal University Xuzhou 221116 China State Key Laboratory of Molecular Engineering of Polymers Department of Macromolecular Science Fudan University Shanghai 200433 China School of Chemistry & Chemical Engineering Anhui University of Technology Ma'anshan Anhui 243002 P. R. China Hefei National Research Center for Physical Sciences at the Microscale Key Laboratory of Strongly-Coupled Quantum Matter Physics of Chinese Academy of Sciences Key Laboratory of Surface and Interface Chemistry and Energy Catalysis of Anhui Higher Education Institutes Department of Chemical Physics University of Science and Technology of China Hefei Anhui 230026 P. R. China Institute of Applied Physics and Materials Engineering University of Macau Macau China

Dual-atom catalysts (DACs) have been proposed to break the limitation of single-atom catalysts (SACs) in the synergistic activation of multiple molecules and intermediates, offering an additional degree of freedom for catalytic regulation. However, it remains a challenge to synthesize DACs with high uniformity, atomic accuracy, and satisfactory loadings. Herein, we report a facile cascade synthetic strategy for DAC via precise electrostatic interaction control and neighboring vacancy construction. We synthesized well-defined, uniformly dispersed dual Fe sites which were connected by two nitrogen bonds (denoted as Fe-N 2 -Fe). The as-synthesized DAC exhibited superior catalytic performances towards oxygen reduction reaction, including good half-wave potential (0.91 V), high kinetic current density (21.66 mA cm −2 ), and perfect durability. Theoretical calculation revealed that the DAC structure effectively tunes the oxygen adsorption configuration and decreases the cleavage barrier, thereby improving the catalytic kinetics. The DAC-based zinc-air batteries exhibited impressive power densities of 169.8 and 52.18 mW cm −2 at 25 °C and −40 °C, which is 1.7 and 2.0 times higher than those based on Pt/C+Ir/C, respectively. We also demonstrated the universality of our strategy in synthesizing other M-N 2 -M DACs (M=Co, Cu, Ru, Pd, Pt, and Au), facilitating the construction of a DAC library for different catalytic applications.

关键词： dual-atom catalyst single-atom catalyst synthetic strategy oxygen reduction reaction zinc-air battery

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Revealing the potential of extracted Tamarindus indica seed as a biocoagulant for aquaculture wastewater treatment: Effect of solvent type, concentration, dose, and toxicity assessment

Results in Engineering

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Results in engineering 2025年 27卷

作者： Setyo Budi Kurniawan Azzahra Aulia Haya Thin Soedarti Eko Prasetyo Kuncoro Łukasz Sługocki Kacper Nowakowski Peer Mohamed Abdul Muhammad Fauzul Imron Department of Chemical and Process Engineering Faculty of Engineering and Built Environment Universiti Kebangsaan Malaysia UKM Bangi Selangor 43600 Malaysia Research Center for Environment and Clean Technology National Research and Innovation Agency (BRIN) Jakarta Pusat 10340 Indonesia Study Program of Environmental Engineering Department of Biology Faculty of Science and Technology Universitas Airlangga Campus C UNAIR Jalan Mulyorejo Surabaya 60115 Indonesia Department of Biology Faculty of Science and Technology Universitas Airlangga Campus C UNAIR Jalan Mulyorejo Surabaya 60115 Indonesia Department of Hydrobiology Institute of Biology University of Szczecin Wąska 13 Szczecin 71-715 Poland Center of Molecular Biology and Biotechnology University of Szczecin Wąska 13 Szczecin 71-715 Poland Sanitary Engineering Section Department of Water Management Faculty of Civil Engineering and Geosciences Delft University of Technology Stevinweg 1 CN Delft 2628 The Netherlands

Aquaculture wastewater contains high levels of organic matter and nutrients, which can be harmful to aquatic life if discharged improperly into surface water bodies. Coagulation-flocculation is currently the best practice for treating aquaculture effluent with biocoagulants, offering an alternative to metal-based coagulants. This study aims to investigate the potential of Tamarindus indica seeds as a biocoagulant for treating aquaculture wastewater, focusing on the optimal solvent extraction, concentration, and dose. This study also examines the toxicity of biocoagulants to aquatic organisms. Coagulation-flocculation study was conducted under jar test experiment with NaCl, NaOH, and HCl used as solvents; concentration of 0-10 g/L; and doses of 1-5 % v/v under 120 rpm (rotation per minute) rapid mixing for 1 min, 20 rpm slow mixing for 20 mins, and 60 mins sedimentation time. A characterization study showed that NaCl-treated T. indica has a positive zeta potential charge, attributed to the presence of hydroxyl, carbonyl, and amide functional groups. Under this optimum condition (NaCl-extract, 6 g/L, and 4 % v/v), the biocoagulant achieved high removal (>50 %) of turbidity, TSS (total suspended solid), and ammonia and considerably good removal of other parameters (TN [total nitrogen], BOD 5 [biological oxygen demand], COD [chemical oxygen demand]). The toxicity test revealed that no mortality was observed at a concentration of 1 g/L, whereas 10 g/L resulted in a 100 % mortality rate after 24 hours of exposure. Further toxicity analysis is suggested to be conducted using treated final effluent (not directly using biocoagulant substances) to observe the direct impact of the treated wastewater if discharged into the water bodies.

关键词： Aquaculture Biocoagulant Coagulation Effluent Environmental pollution Flocculation

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Enriching and Characterizing T-Cell Repertoires from 3' Barcoded Single-Cell Whole Transcriptome Amplification Products

arXiv

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arXiv 2022年

作者： Jivanjee, Tasneem Ibrahim, Samira Nyquist, Sarah K. James Gatter, G. Bromley, Joshua D. Jaiswal, Swati Berger, Bonnie Behar, Samuel M. Christopher Love, J. Shalek, Alex K. Institute for Medical Engineering & Science Department of Chemistry Massachusetts Institute of Technology CambridgeMA United States Koch Institute for Integrative Cancer Research Massachusetts Institute of Technology CambridgeMA United States Ragon Institute of MGH MIT and Harvard CambridgeMA United States Broad Institute of MIT and Harvard CambridgeMA United States Massachusetts Institute of Technology CambridgeMA United States Program in Computational and Systems Biology Massachusetts Institute of Technology CambridgeMA United States Computer Science and Artificial Intelligence Laboratory Massachusetts Institute of Technology CambridgeMA United States Microbiology Graduate Program Massachusetts Institute of Technology CambridgeMA02139 United States Department of Microbiology and Physiological Systems University of Massachusetts Medical School WorcesterMA United States Department of Mathematics Massachusetts Institute of Technology CambridgeMA United States Department of Chemical Engineering Massachusetts Institute of Technology CambridgeMA United States

Antigen-specific T cells play an essential role in immunoregulation and many diseases such as cancer. Characterizing the T cell receptor (TCR) sequences that encode T cell specificity is critical for elucidating the antigenic determinants of immunological diseases and designing therapeutic remedies. However, methods of obtaining single-cell TCR sequencing data are labor and cost intensive, typically requiring both cell sorting and full length single-cell RNA-sequencing (scRNA-seq). New high-throughput 3' cell-barcoding scRNA-seq methods can simplify and scale this process;however, they do not routinely capture TCR sequences during library preparation and sequencing. While 5' cell-barcoding scRNA-seq methods can be used to examine TCR repertoire at single-cell resolution, doing so requires specialized reagents which cannot be applied to samples previously processed using 3' cell-barcoding methods. Here, we outline a method for sequencing TCRα and TCRβ transcripts from samples already processed using 3' cell-barcoding scRNA-seq platforms, ensuring TCR recovery at a single-cell resolution. In short, a fraction of the 3' barcoded whole transcriptome amplification (WTA) product typically used to generate a massively parallel 3' scRNA-seq library is enriched for TCR transcripts using biotinylated probes, and further amplified using the same universal primer sequence from WTA. Primer extension using TCR V-region primers and targeted PCR amplification using a second universal primer results in a 3' barcoded single-cell CDR3-enriched library that can be sequenced with custom sequencing primers. Coupled with 3' scRNA-seq of the same WTA, this method enables simultaneous analysis of single-cell transcriptomes and TCR sequences which can help interpret inherent heterogeneity among antigen-specific T cells and salient disease biology. The method presented here can also be adapted readily to enrich and sequence other transcripts of interest from both 3' and 5' barcoded scRNA-seq

关键词： RNA

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Corrigendum to “Phosphazene-based covalent organic polymers as metal-free catalysts with improved H 2 generation from NaBH 4 in methanol with superior catalytic activity and re-generation ability” [J. Environ. Chem. Eng. 12 2 (2024) 112066]

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Journal of Environmental chemical engineering 2024年第6期12卷

作者： Sahin Demirci Mustafa Yildiz Nurettin Sahiner Department of Chemistry Faculty of Sciences and Nanoscience and Technology Research and Application Center (NANORAC) Canakkale Onsekiz Mart University Terzioglu Campus Canakkale 17100 Turkey Department of Ophthalmology Morsani College of Medicine University of South Florida Eye Institute 12901 Bruce B Down Blvd MDC 21 Tampa FL 33612 USA Department of Chemical & Biomedical Engineering Director Materials Science and Engineering Program University of South Florida Tampa FL 33620 USA

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DeePMD-kit v2: A software package for Deep Potential models

arXiv

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arXiv 2023年

作者： Zeng, Jinzhe Zhang, Duo Lu, Denghui Mo, Pinghui Li, Zeyu Chen, Yixiao Rynik, Marián Huang, Li'ang Li, Ziyao Shi, Shaochen Wang, Yingze Ye, Haotian Tuo, Ping Yang, Jiabin Ding, Ye Li, Yifan Tisi, Davide Zeng, Qiyu Bao, Han Xia, Yu Huang, Jiameng Muraoka, Koki Wang, Yibo Chang, Junhan Yuan, Fengbo Bore, Sigbjørn Løland Cai, Chun Lin, Yinnian Wang, Bo Xu, Jiayan Zhu, Jia-Xin Luo, Chenxing Zhang, Yuzhi Goodall, Rhys E.A. Liang, Wenshuo Singh, Anurag Kumar Yao, Sikai Zhang, Jingchao Wentzcovitch, Renata Han, Jiequn Liu, Jie Jia, Weile York, Darrin M. Weinan, E. Car, Roberto Zhang, Linfeng Wang, Han Laboratory for Biomolecular Simulation Research Institute for Quantitative Biomedicine Department of Chemistry and Chemical Biology Rutgers University PiscatawayNJ08854 United States AI for Science Institute Beijing100080 China DP Technology Beijing100080 China Academy for Advanced Interdisciplinary Studies Peking University Beijing100871 China HEDPS CAPT College of Engineering Peking University Beijing100871 China College of Electrical and Information Engineering Hunan University Changsha China Yuanpei College Peking University Beijing100871 China Program in Applied and Computational Mathematics Princeton University PrincetonNJ08540 United States Department of Experimental Physics Comenius University Mlynská Dolina F2 Bratislava842 48 Slovakia Center for Quantum Information Institute for Interdisciplinary Information Sciences Tsinghua University Beijing100084 China Center for Data Science Peking University Beijing100871 China ByteDance Research Zhonghang Plaza No. 43 North 3rd Ring West Road Haidian District Beijing China College of Chemistry and Molecular Engineering Peking University Beijing100871 China Baidu Inc. Beijing China Key Laboratory of Structural Biology of Zhejiang Province School of Life Sciences Westlake University Zhejiang Hangzhou China Westlake AI Therapeutics Lab Westlake Laboratory of Life Sciences and Biomedicine Zhejiang Hangzhou China Department of Chemistry Princeton University PrincetonNJ08544 United States SISSA Scuola Internazionale Superiore di Studi Avanzati Trieste34136 Italy Laboratory of Computational Science and Modeling Institute of Materials École Polytechnique Fédérale de Lausanne Lausanne1015 Switzerland Department of Physics National University of Defense Technology Hunan Changsha410073 China State Key Lab of Processors Institute of Computing Technology Chinese Academy of Sciences Beijing China University of Chinese Academy of Sciences Beijing China School of Electronics Engineerin

DeePMD-kit is a powerful open-source software package that facilitates molecular dynamics simulations using machine learning potentials (MLP) known as Deep Potential (DP) models. This package, which was released in 2017, has been widely used in the fields of physics, chemistry, biology, and material science for studying atomistic systems. The current version of DeePMD-kit offers numerous advanced features such as DeepPot-SE, attention-based and hybrid descriptors, the ability to fit tensile properties, type embedding, model deviation, Deep Potential - Range Correction (DPRc), Deep Potential Long Range (DPLR), GPU support for customized operators, model compression, non-von Neumann molecular dynamics (NVNMD), and improved usability, including documentation, compiled binary packages, graphical user interfaces (GUI), and application programming interfaces (API). This article presents an overview of the current major version of the DeePMD-kit package, highlighting its features and technical details. Additionally, the article benchmarks the accuracy and efficiency of different models and discusses ongoing developments. © 2023, CC BY-NC-ND.

关键词： Molecular dynamics

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Converting hydroponic effluent into high PUFA-rich biomass by mixotrophically cultivated microalga Chlorella sp. through light regulation for sustainable production of bioingredient and bioenergy

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Journal of Environmental chemical engineering 2025年第3期13卷

作者： Nanthakrit Sriket Antira Wichaphian Apiwit Kamngoen Jeeraporn Pekkoh Wasu Pathom-aree Yupa Chromkaew Benjamas Cheirsilp Wageeporn Maneechote Yoong Kit Leong Sirasit Srinuanpan Master of Science Program in Applied Microbiology (International Program) Department of Biology Faculty of Science Chiang Mai University Chiang Mai 50200 Thailand Department of Biology Faculty of Science Chiang Mai University Chiang Mai 50200 Thailand Microbial Biorefinery and Biochemical Process Engineering Research Group Chiang Mai University Chiang Mai 50200 Thailand Center of Excellence in Microbial Diversity and Sustainable Utilization Chiang Mai University Chiang Mai 50200 Thailand Department of Plant and Soil Sciences Faculty of Agriculture Chiang Mai University Chiang Mai 50200 Thailand Program of Biotechnology Faculty of Agro-Industry Prince of Songkla University Hat Yai Songkhla 90110 Thailand Office of Research Administration Office of the University Chiang Mai University Chiang Mai 50200 Thailand Department of Chemical and Materials Engineering Tunghai University Taichung 407 Taiwan

Currently, addressing global environmental challenges—particularly the management of nutrients and resource recovery from hydroponic effluent (HE)—is crucial for promoting sustainable agriculture and fostering circularity in the pursuit of a net-zero future. This study aimed to evaluate the potential of Chlorella sp. to convert HE into high-value biomass rich in polyunsaturated fatty acids (PUFAs) under mixotrophic conditions with optimized light illumination. The results demonstrated that supplementing HE with half the concentration of commercial JM medium, and optimizing with blue light, a 16-hour light/8-hour dark photoperiod, and a light intensity of 50 µmol/m 2 /s resulted in a biomass production of 4.04 g/L, containing 16.81 % lipids and 34.04 % PUFAs. These conditions also achieved significant pollutant removal from HE, including 93.33 % nitrate reduction, 100 % ammonia removal, 99.08 % phosphate removal, and 92.68 % reduction in chemical oxygen demand. The nutritional profile of the fatty acids suggests potential applications in the functional ingredient sector, while their characteristics indicate strong potential as biodiesel feedstock, meeting international biodiesel fuel standards. Overall, this study presents an eco-friendly model for nutrient recovery from hydroponic effluent using Chlorella sp., promoting sustainable agriculture, reducing environmental impact, and contributing to bioresource innovation.

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Electrically powered locomotion of dual-nature colloidhedgehog and colloid-umbilic topological and elastic dipoles in liquid crystals

arXiv

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arXiv 2021年

作者： Senyuk, Bohdan Adufu, Richmond E. Smalyukh, Ivan I. Department of Physics University of Colorado BoulderCO80309 United States Department of Electrical Computer and Energy Engineering University of Colorado BoulderCO80309 United States Soft Materials Research Center Materials Science and Engineering Program University of Colorado BoulderCO80309 United States Chemical Physics Program Departments of Chemistry and Physics University of Colorado BoulderCO80309 United States Renewable and Sustainable Energy Institute National Renewable Energy Laboratory University of Colorado BoulderCO80309 United States

Colloidal particles in liquid crystals tend to induce topological defects and distortions of the molecular alignment within the surrounding anisotropic host medium, which results in elasticity-mediated interactions not accessible to their counterparts within isotropic fluid hosts. Such particle-induced coronae of perturbed nematic order are highly responsive to external electric fields, even when the uniformly aligned host medium away from particles exhibits no response to fields below the realignment threshold. Here we harness the non-reciprocal nature of these facile electric responses to demonstrate colloidal locomotion. Oscillations of electric field prompt repetitive deformations of the corona of dipolar elastic distortions around the colloidal inclusions, which at appropriately designed electric driving synchronize the displacement directions. We observe the colloid-hedgehog dipole accompanied by an umbilical defect in the tilt directionality field (c-field), along with the texture of elastic distortions that evolves with changing applied voltage. The temporal out-of-equilibrium evolution of the director and c-field distortions around particles upon turning voltage on and off is not invariant upon reversal of time, prompting lateral translations and interactions that markedly differ from what is accessible to these colloids under equilibrium conditions. Our findings may lead to both technological and fundamental science applications of nematic colloids as both model reconfigurable colloidal systems and as mesostructured materials with pre-designed temporal evolution of structure and composition. © 2021, CC BY-NC-ND.

关键词： Liquid crystals

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Conjugated and non-conjugated multifunctional electrochromic polymers containing triarylamine blocks

Conjugated and non-conjugated multifunctional electrochromic...

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中国化学会第二十次全国电化学大会

作者： Niu Haijun Zhang Xu Lu Qingyi Zheng Rongrong Yang Caiyu Chen Ye Key Laboratory of Functional Inorganic Material Chemistry Ministry of Education Department of Macromolecular Science and Engineering School of Chemical Chemical Engineering and Materials Heilongjiang University

Electrochromism is known as the alternation of color by the application of a potential. Color changes are commonly occur between a transparent state, where the chromophore only absorbs in the UV region, and a colored state or between two colored states in a given electrolyte *** materials have extensive application prospects as intelligent materials and have been used for smart windows, anti-glass and optical displays.

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