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Improvement of a highly sensitive and specific whole-cell biosensor by adding a positive feedback amplifier

Synthetic and systems Biotechnology 2023年第2期8卷 292-299页

作者： Shuting Hu Guangbao Zhang Xiaoqiang Jia Department of Biochemical Engineering School of Chemical Engineering and TechnologyTianjin UniversityTianjin300072PR China Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education) School of Chemical Engineering and TechnologyTianjin UniversityTianjin300350PR China Collaborative Innovation Center of Chemical Science and Engineering(Tianjin) Tianjin300072PR China

In this study,we designed a Cd^(2+)whole-cell biosensor with both positive and negative feedback cascade am-plifiers in Pseudomonas putida KT2440(LTCM)based on our previous design with only a negative feedback amplifier(TCM).The results showed that the newly developed biosensor LTCM was greatly improved compared to ***,the linear response range of LTCM was expanded while the maximum linear response range was raised from 0.05 to 0.1μ***,adding a positive feedback amplifier further increased the fluorescence output signal of LTCM 1.11–2.64 times under the same culture ***,the response time of LTCM for detection of practical samples was reduced from 6 to 4 *** the same time,LTCM still retained very high sensitivity and specificity,while its lowest detection limit was 0.1 nM Cd^(2+)and the specificity was 23.29(compared to 0.1 nM and 17.55 in TCM,respectively).In summary,the positive and negative feedback cascade amplifiers effectively improved the performance of the biosensor LTCM,resulting in a greater linear response range,higher output signal intensity,and shorter response time than TCM while retaining comparable sensitivity and specificity,indicating better potential for practical applications.

关键词： Cadmium detection Positive feedback amplifier Sensitivity Specificity Whole-cell biosensor

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Insights into the cross-amyloid aggregation of Aβ_(40) and its N-terminal truncated peptide Aβ_(11-40) affected by epigallocatechin gallate

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Chinese Journal of chemical engineering 2022年第5期35卷 284-293页

作者： Yue Liang Wenjuan Wang Yan Sun Xiaoyan Dong Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering(Ministry of Education) School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300354China

Inhibition of protein misfolding and aggregation is a great challenge in the field of biochemical and biopharmaceutical ***’s disease(AD)is a protein-misfolding disease,and the interactions between 40-amino-acid-residueβ-amyloid peptide(Aβ_(40)) and its N-terminal truncated peptide Aβ_(11-40) demonstrate that Aβ_(11-40) may play an important role in the pathological process of ***,the effect of inhibitors on Aβ_(11-40) aggregation and on the cross-amyloid aggregation(coassembly)between Aβ_(40) and Aβ_(11-40) has never been ***,coaggregation and seeding interactions between Aβ_(40) and Aβ_(11-40) as well as the effect of epigallocatechin gallate(EGCG),a small molecule inhibitor,on the cross-amyloid aggregation have been investigated by extensive *** is found that Aβ_(11-40) participates in the aggregation of Aβ_(40) and leads to the formation of coaggregates that contain lessβ-sheet structures than pure Aβ_(40) *** aggregation kinetics along with morphologies and secondary structures of the coaggregates are also significantly affected by the Aβ_(40)/Aβ_(11-40) *** accelerates the nucleation of Aβ_(40) but retards that of Aβ_(11-40) by affecting their elongation and secondary nucleation processes in solution and on solid ***,EGCG makes the conformations of the seeding-induced Aβaggregates more compact,especially for the homologous *** titration calorimetry measurement indicates that hydrophobic interactions mainly contribute to the inhibition of the two Aβisoforms by *** findings of this research have provided new insights into Aβaggregation and the effect of an important inhibitor and the results would benefit in the development of potent inhibitors against co-assembly of different amyloid proteins.

关键词： Protein Aggregation Kinetics Nucleation Elongation Molecular interactions

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Catalytically active membranes for esterification:A review

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Chinese Journal of chemical engineering 2023年第1期53卷 142-154页

作者： Monique Juna L.Leite Ingrid Ramalho Marques Mariane Carolina Proner Pedro H.H.Araújo Alan Ambrosi Marco Di Luccio Laboratory of Membrane Processes LABSEMDepartment of Chemical and Food EngineeringFederal University of Santa Catarina88040-970 FlorianópolisSCBrazil Laboratory of Control and Polymerization Processes LCPDepartment of Chemical and Food EngineeringFederal University of Santa Catarina88040-970 FlorianópolisSCBrazil

Esterification is an important process in the food industry and can be carried out via homogeneous or heterogeneous *** homogeneous catalyst,despite providing high conversion,can cause corrosion in reactors,which is not observed with the use of heterogeneous ***,some of these catalysts require a high process temperature and may lose their catalytic activity with ***,catalytic membranes have been proposed as a promising *** combination of catalysis and separation in a single module provides greater conversion,reduction of excess reagents,compact industrial plant,making the process more *** this context,this work aims to present a literature review on the catalytic membrane for the synthesis of esters,improving the understanding of the production and *** review examines the materials,catalysts used,and synthetic pathways.A comparison between the methods,as well as limitations and gaps in the literature,are highlighted.

关键词： Catalytic membrane Esterification Optimization Pervaporation Separation Selectivity

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A novel responsive stabilizing Janus nanosilica as a nanoplugging agent in water-based drilling fluids for exploiting hostile shale environments

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Petroleum Science 2024年第2期21卷 1190-1210页

作者： Alain Pierre Tchameni Lv-Yan Zhuo Lesly Dasilva Wandji Djouonkep Robert Dery Nagre Lu-Xin Chen Lin Zhao Chao Ma Bin-Qiang Xie College of Petroleum Engineering Yangtze UniversityWuhan430100HubeiChina Key Laboratory of Drilling and Production Engineering for Oil and Gas HubeiChina Lost Circulation Control Laboratory National Engineering Laboratory for Petroleum Drilling EngineeringYangtze UniversityWuhan430100HubeiChina College of Petroleum Engineering Shandong Institute of Petroleum and Chemical TechnologyDongying257061ShandongChina Petroleum Engineer Research Institute of Petrochina Dagang Oilfield Tianjin300280China Department of Chemical Engineering Kumasi Technical UniversityP.O.Box 854KumasiGhana

Thermo-responsive nanocomposites have recently emerged as potential nanoplugging agents for shale stabilization in high-temperature water-based drilling fluids(WBDFs). However, their inhibitory properties have not been very effective in high-temperature drilling operations. Thermo-responsive Janus nanocomposites are expected to strongly interact with clay particles from the inward hemisphere of nanomaterials, which drive the establishment of a tighter hydrophobic membrane over the shale surface at the outward hemisphere under geothermal conditions for shale stabilization. This work combines the synergistic benefits of thermo-responsive and zwitterionic nanomaterials to synchronously enhance the chemical inhibitions and plugging performances in shale under harsh conditions. A novel thermoresponsive Janus nanosilica(TRJS) exhibiting zwitterionic character was synthesized, characterized,and assessed as shale stabilizer for WBDFs at high temperatures. Compared to pristine nanosilica(Si NP)and symmetrical thermo-responsive nanosilica(TRS), TRJS exhibited anti-polyelectrolyte behaviour, in which electrolyte ions screened the electrostatic attraction between the charged particles, potentially stabilizing nanomaterial in hostile shaly environments(i.e., up to saturated brine or API brine). Macroscopically, TRJS exhibited higher chemical inhibition than Si NP and TRS in brine, prompting a better capability to control pressure penetration. TRJS adsorbed onto the clay surface via chemisorption and hydrogen bonding, and the interactions became substantial in brine, according to the results of electrophoretic mobility, surface wettability, and X-ray diffraction. Thus, contributing to the firm trapping of TRJS into the nanopore structure of the shale, triggering the formation of a tight hydrophobic membrane over the shale surface from the outward hemisphere. The addition of TRJS into WBDF had no deleterious effect on fluid properties after hot-treatment at 190℃, implying that TRJ

关键词： Janus nanosilica Thermo-responsive copolymer Anti-polyelectrolyte effect Shale stabilizer Inhibition Plugging Drilling fluid

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Sigma-Mixed Unscented Kalman Filter-Based Fault Detection for Traction systems in High-Speed Trains

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Chinese Journal of Electronics 2023年第5期32卷 982-991页

作者： CHENG Chao WANG Weijun MENG Xiangxi SHAO Haidong CHEN Hongtian Department of Computer Science and Technology Changchun University of Technology Department of Mathematics and Statistics Changchun University of Technology Institute of System Research China Industrial Control Systems Cyber Emergency Response Team Department of Mechanical and Vehicle Engineering Hunan University Department of Chemical and Materials Engineering University of Alberta

Fault detection(FD) for traction systems is one of the active topics in the railway and academia because it is the initial step for the running reliability and safety of high-speed trains. Heterogeneity of data and complexity of systems have brought new challenges to the traditional FD methods. For addressing these challenges, this paper designs an FD algorithm based on the improved unscented Kalman filter(UKF) with consideration of performance degradation. It is derived by incorporating a degradation process into the state-space *** network topology of traction systems is taken into consideration for improving the performance of state estimation. We first obtain the mixture distribution by the mixture of sigma points in UKF. Then, the Lévy process with jump points is introduced to construct the degradation model. Finally, the moving average interstate standard deviation(MAISD) is designed for detecting *** the proposed methods via a traction systems in a certain type of trains obtains satisfactory results.

关键词： Degradation Network topology Fault detection Rail transportation State-space methods Safety Kalman filters

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Development of biology learning programs for entrepreneurship through microbiology lectures in educational institutions of the UK: Explanatory research

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Journal of Commercial Biotechnology 2023年第5期28卷 168-181页

作者： Janssen, Lisa Metrakos, Stephanie The Sargent Centre for Process Systems Engineering Imperial College London London United Kingdom Department of Chemical Engineering Imperial College London London United Kingdom

This explanatory research examines the development of biology learning programs for entrepreneurship through microbiology lectures in UK educational institutions. Specifically, it investigates the impact of bio-entrepreneurship learning and problem-based learning on students' attitudes towards bio-entrepreneurship, considering mediating roles of teaching styles and learners' interests. The quantitative methodology was adopted, with 244 students completing a survey questionnaire. Results showed bio-entrepreneurship learning and problem-based learning positively influence attitudes towards bio-entrepreneurship. Furthermore, teaching styles significantly mediated the relationship between learning approaches and attitudes. However, learners' interests did not mediate these associations. The study provides theoretical contributions on intersecting microbiology education and entrepreneurship. Practical implications highlight designing customized bio-entrepreneurship curricula by leveraging problem-based learning and effective instructional techniques. Limitations include contextual focus on the UK and lacking analysis of actual entrepreneurial activities. Further research should examine long-term impacts on career pursuits and compare cross-national effectiveness. © 2023, Journal of Commercial Biotechnology. All rights reserved.

关键词： Microbiology

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Social NSTransformers: Low-Quality Pedestrian Trajectory Prediction

IEEE Transactions on Artificial Intelligence

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IEEE Transactions on Artificial Intelligence 2024年第11期5卷 5575-5588页

作者： Jiang, Zihan Ma, Yiqun Shi, Bingyu Lu, Xin Xing, Jian Goncalves, Nuno Jin, Bo Suzhou215000 China Northeast Forestry University College of Computer and Control Engineering Harbin150000 China University of Coimbra Institute of Systems and Robotics Department of Electrical and Computer Engineering Coimbra3030-290 Portugal

This article introduces a novel model for low-quality pedestrian trajectory prediction, the social nonstationary transformers (NSTransformers), that merges the strengths of NSTransformers and spatiotemporal graph transformer (STAR). The model can capture social interaction cues among pedestrians and integrate features across spatial and temporal dimensions to enhance the precision and resilience of trajectory predictions. We also propose an enhanced loss function that combines diversity loss with logarithmic root mean squared error (log-RMSE) to guarantee the reasonableness and diversity of the generated trajectories. This design adapts well to complex pedestrian interaction scenarios, thereby improving the reliability and accuracy of trajectory prediction. Furthermore, we integrate a generative adversarial network (GAN) to model the randomness inherent in pedestrian trajectories. Compared to the conventional standard Gaussian distribution, our GAN approach better simulates the intricate distribution found in pedestrian trajectories, enhancing the trajectory prediction's diversity and robustness. Experimental results reveal that our model outperforms several state-of-the-art methods. This research opens the avenue for future exploration in low-quality pedestrian trajectory prediction. © 2024 IEEE.

关键词： Stars

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Creating and Studying a Methodology for Calculating a Resistive Voltage Divider for Use in Electronic Voltage Transformers 7

Creating and Studying a Methodology for Calculating a Resist...

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7th International Scientific and Technical Conference Relay Protection and Automation, RPA 2024

作者： Yablokov, A.A. Panashchatenko, A.V. Gotovkina, E.E. Maltsev, R.A. Ivanovo State Power Engineering University Electric Power Systems Automatic Control Department Electric Systems Department Ivanovo Russia

ISBN: (纸本)9798331501105

In 2017 the Russian government approved a programme, Russian Federation Digital Economy, the main goal of which is to create an ecosystem with large scale use of digital format data in all fields of social and economic activity [1]. The electric power industry is being transformed in accordance with the concept, Digital Transformation 2030, developed by Russian Power Grids PLC [2]. Pursuant to this concept, electric substations under construction should be digital. At present, Russia has a number of operating digital substations (DSS) (Medvedevskaya SS, UVAT SS, Desna SS, Tobol SS [3]-[5], while many DSSs are at the design or construction stage. The creation of new substations, and also reconstruction of already operating ones, requires solving the problems of operating conventional electromagnetic current and voltage transformers. Electromagnetic voltage transformers (VT) are energy-inefficient, explosion - and flammability- hazardous equipment (due to their requiring additional charging with low consumption by micro-processing devices), with large weight and dimensions (an NKF-100 type transformer weighing 840 kg). © 2024 IEEE.

关键词： Distribution transformers

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A NOTE ON THE QUALITATIVE BEHAVIOUR OF AN ODE MODEL RELATED TO ENDOTOXIN TOLERANCE

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UPB Scientific Bulletin, Series A: Applied Mathematics and Physics 2024年第4期86卷 127-130页

作者： Olteanu, Mircea Ştefan, Radu Department of Mathematical Methods and Models Faculty of Applied Sciences University "Politehnica" of Bucharest Romania Department of Automatic Control and Systems Engineering Faculty of Automatic Control and Computer Science University "Politehnica" of Bucharest Romania

关键词： Differential equations

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Understanding the Thermal Impedance of Silicone Rubber/Hexagonal Boron Nitride Composites as Thermal Interface Materials

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Chinese Journal of Polymer Science 2024年第3期42卷 352-363,I0008页

作者： Yuan Ji Shi-Da Han Hong Wu Shao-Yun Guo Feng-Shun Zhang Jian-Hui Qiu The State Key Laboratory of Polymer Materials Engineering Sichuan Provincial Engineering Laboratory of Plastic/Rubber Complex Processing TechnologyPolymer Research InstituteSichuan UniversityChengdu 610065China Institute of Chemical Materials China Academy of Engineering PhysicsMianyang 621900China Department of Mechanical Engineering Faculty of Systems Science and TechnologyAkita Prefectural UniversityAkita 015-0055Japan

Silicone rubber(SR) composites are most widely used as thermal interface materials(TIMs) for electronics heat dissipation. Thermal impedance as the main bottleneck limiting the performance of TIMs is usually neglected. Herein, the thermal impedance of SR composites loaded with different levels of hexagonal boron nitride(h-BN) as TIMs was elaborated for the first time by the ASTM D 5470 standard test and finite element analysis. It was found that elastic modulus and surface roughness of SR composites increased with the increase of h-BN content, indicating that the conformity was reduced. When the assembly pressure was 0.69 MPa, there existed an optimal h-BN content at which the contact resistance was minimum(0.39 K·cm^(2)·W^(-1)). Although the decreased bond line thickness(BLT) by increasing the assembly pressure was beneficial to reduce the thermal impedance, the proper assembly pressure should be selected to prevent the warpage of the contact surfaces and the increase in contact resistance, according to the compression properties of the SR composites. This study provides valuable insights into fabrication of high-performance TIMs for modern electronic device applications.

关键词： Thermal interface materials Hexagonal boron nitride Thermal impedance Surfaces

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