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Angiogenesis driven extracellular matrix remodeling of 3D bioprinted vascular networks

Bioprinting 2023年 30卷

作者： Li, Ying Betty Sodja, Caroline Rukhlova, Marina Nhan, Jordan Poole, Joshua J.A. Allen, Harry Yimer, Selam Baumann, Ewa Bedford, Erin Prazak, Hannah Costain, Will J. Murugkar, Sangeeta St-Pierre, Jean-Philippe Mostaço-Guidolin, Leila Jezierski, Anna Human Health Therapeutics Research Centre National Research Council of Canada Ottawa ON Canada University of Ottawa Department of Chemical and Biological Engineering Faculty of Engineering Ottawa ON Canada Carleton University Department of Systems and Computer Engineering Faculty of Engineering and Design Ottawa ON Canada Carleton University Department of Physics Faculty of Science Ottawa ON Canada University of Ottawa Department of Biomedical Science Faculty of Engineering Ottawa ON Canada Aspect Biosystems Inc. Vancouver BC Canada University of Ottawa Department of Biochemistry Microbiology and Immunology Faculty of Medicine Ottawa ON Canada

Angiogenesis plays a pivotal role in development and tissue growth, as well as in pathological conditions such as cancer. Being able to understand the basic mechanisms involved in the vascularization of tissues and angiogenic network formation provides a window to advance the development of in vitro tissue models and enhance tissue engineering applications. In this study, we leveraged a novel microfluidic-based three dimensional (3D) bioprinting technology and alginate-collagen type I (AGC) bioink, to develop a 3D bioprinting strategy to enable the biofabrication of complex angiogenic networks within the 3D structure. These networks were comprised of simian vacuolating virus 40 (SV40) transformed adult rat brain endothelial cell (SV-ARBEC)-laden hydrogel rings. With mechanical properties relevant for vascular tissue engineering applications, these bioprinted constructs formed spontaneous vascular networks, reminiscent of anisotropic tissue-like structures, while retaining high cellular viability. The vascular network formation was accompanied by extracellular matrix (ECM) remodeling, confirming sequential SV-ARBEC mediated collagen type I fiber deposition and reorganization. Treatment with broad spectrum matrix metalloproteinase (MMP) inhibitor supressed SV-ARBEC angiogenic sprouting, highlighting requirements of ECM remodeling in angiogenic network formation. This novel 3D microfluidic bioprinting technology and biocompatible AGC hydrogel fiber rings supported robust SV-ARBEC angiogenesis and corresponding ECM remodeling, allowing us to present a strategy suitable to advancing applications in vascular research and supporting the further development of disease models, novel testing beds for drug discovery and tissue engineering applications. © 2023

关键词： 3D bioprinting Alginate Angiogenesis Bioink Collagen ECM Remodeling Microfluidics Vascular networks

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Is monochloramine pre-oxidation a viable strategy for enhancing the treatment efficiency of algae-laden water with conventional drinking water treatment process?

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Chemosphere 2024年 352卷 141312页

作者： Liu, Han Lv, Huanyu Xu, Hangzhou Rao, Dandan Zhang, Jian Sun, Bo Shandong Key Laboratory of Water Pollution Control and Resource Reuse School of Environmental Science & Engineering Shandong University Shandong Qingdao 266237 China Department of Chemical and Environmental Engineering University of California Riverside A235 Bourns Hall 3401 Watkins Drive Riverside 92521 CA United States College of Safety and Environmental Engineering Shandong University of Science and Technology Shandong Qingdao 266590 China

Algal blooms worldwide pose many challenges to drinking water production. Pre-oxidation with NaClO, KMnO4, or ozone is commonly used to enhance algal removal in conventional drinking water treatment processes. However... 详细信息

Algal blooms worldwide pose many challenges to drinking water production. Pre-oxidation with NaClO, KMnO₄, or ozone is commonly used to enhance algal removal in conventional drinking water treatment processes. However, these currently utilized oxidation methods often result in significant algal cell lysis or impede the operation of the subsequent units. Higher algal removal with pre-chlorination in algal solutions prepared with natural water, compared to those prepared with ultrapure water, has been observed. In the present studies, preliminary findings indicate that ammonium in natural water alters chlorine species to NH₂Cl, leading to improved treatment efficiency. NH₂Cl with 1.5–3.0 mg∙L⁻¹ as Cl₂ with an oxidation time of 3–7 h significantly enhancing algal removal by coagulation. The selective oxidation of surface-absorbed organic matter (S-AOM) by NH₂Cl, followed by the subsequent peeling off of this material from the algal surface, leading to an increase in zeta potential from −20.2 mV to −3.8 mV, constitutes the primary mechanism of enhanced algal removal through coagulation. These peeled S-AOM retained their large molecular weight and acted as polymer aids. Compared with NaClO and KMnO₄, NH₂Cl displays the best performance in improving algal removal, avoiding cell lysis, and decreasing the potential for nitrogenous disinfection byproducts formation under the reaction conditions used in this study. Notably, in major Chinese cities, water purification plants commonly rely on suburban lakes or reservoirs as water sources, necessitating the transportation of raw water over long distances for times up to several hours. These conditions favor the implementation of NH₂Cl pre-oxidation. The collective results indicate the potential of NH₂Cl oxidation as a viable pretreatment strategy for algal contamination during water treatment processes. © 2024 Elsevier L

关键词： Algal removal Enhanced coagulation Monochloramine Pre-oxidation

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Exponential Consensus of Multiple Agents over Dynamic Network Topology: controllability, Connectivity, and Compactness

arXiv

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arXiv 2023年

作者： Ma, Qichao Qin, Jiahu Anderson, Brian D.O. Wang, Long Department of Automation University of Science and Technology of China Hefei230027 China Institute of Artificial Intelligence Hefei Comprehensive National Science Center Hefei230088 China School of Engineering Australian National University ActonACT2601 Australia Center for Systems and Control College of Engineering Peking University Beijing100871 China

This paper investigates the problem of securing exponentially fast consensus (exponential consensus for short) for identical agents with finite-dimensional linear system dynamics over dynamic network topology. Our aim is to find the weakest possible conditions that guarantee exponentially fast consensus using a Lyapunov function consisting of a sum of terms of the same functional form. We first investigate necessary conditions, starting by examining the system (both agent and network) parameters. It is found that controllability of the linear agents is necessary for reaching consensus. Then, to work out necessary conditions incorporating the network topology, we construct a set of Laplacian matrix-valued functions. The precompactness of this set of functions is shown to be a significant generalization of existing assumptions on network topology, including the common assumption that the edge weights are bounded piecewise constant functions or continuous functions. With the aid of such a precompactness assumption and restricting the Lyapunov function to one consisting of a sum of terms of the same functional form, we prove that a joint (δ, T)-connectivity condition on the network topology is necessary for exponential consensus. Finally, we investigate how the above two "necessities" work together to guarantee exponential consensus. To partially address this problem, we define a synchronization index to characterize the interplay between agent parameters and network topology. Based on this notion, it is shown that by designing a proper feedback matrix and under the precompactness assumption, exponential consensus can be reached globally and uniformly if the joint (δ, T)-connectivity and controllability conditions are satisfied, and the synchronization index is not less than one. © 2023, CC BY-NC-ND.

关键词： Network topology

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Constructing electron transport channel of SnO2/BaSO4 on g-C3N4 for enhanced visible-light-driven photocatalytic H2 production

Materials Today Catalysis

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Materials Today Catalysis 2025年 9卷

作者： Dong, Gongyue Guo, Xiaojuan Cheng, Cheng Chen, Feng Zhang, Jinfeng Du, Yanping Meng, Wei Liu, Maochang Shi, Jinwen International Research Center for Renewable Energy State Key Laboratory of Multiphase Flow in Power Engineering Xi'an Jiaotong University Xi'an710049 China Guangdong Provincial Key Laboratory of Multi-energy Complementary Distributed Energy Systems Dongguan University of Technology Dongguan523808 China School of Engineering Lancaster University LancasterLA1 4YW United Kingdom Gansu Company PipeChina Lanzhou730060 China Hunan Provincial Key Lab of Dark Tea and Jin-Hua Department of Material & Chemical Engineering Hunan City University Yiyang413000 China

Graphitic carbon nitride (g-C3N4) is confronted with the issue of poor utilization of photogenerated charge carriers, thereby leading to limited performance of photocatalytic hydrogen (H2) production, which restricts its potential application. Herein, the electron transport material SnO2/BaSO4 was synthesized to integrate with g-C3N4 for addressing the above problem. Various characterizations were conducted to investigate the g-C3N4-SnO2/BaSO4 photocatalyst, and it demonstrated that photogenerated electrons from g-C3N4 expeditiously migrate to SnO2/BaSO4 nanoparticles, which markedly hindered photogenerated carriers’ recombination. Subsequently, the g-C3N4-SnO2/BaSO4 photocatalyst demonstrated promoted photocatalytic H2 production at a rate of 14.2 μmol h−1 under visible-light illumination, which was 2.5 times higher than that of pristine g-C3N4. © 2025 The Authors

关键词： Barium sulfate

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Data-driven Polytopic Output Synchronization of Heterogeneous Multi-agent systems from Noisy Data

arXiv

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arXiv 2023年

作者： Li, Yifei Liu, Wenjie Sun, Jian Wang, Gang Xie, Lihua Chen, Jie The National Key Lab of Autonomous Intelligent Unmanned Systems Beijing Institute of Technology Beijing100081 China The Beijing Institute of Technology Chongqing Innovation Center Chongqing401120 China School of Electrical and Electronic Engineering Nanyang Technological University Singapore The Department of Control Science and Engineering Tongji University Shanghai201804 China

This paper proposes a novel approach to addressing the output synchronization problem in unknown heterogeneous multi-agent systems (MASs) using noisy data. Unlike existing studies that focus on noiseless data, we introduce a distributed data-driven controller that enables all heterogeneous followers to synchronize with a leader’s trajectory. To handle the noise in the state-input-output data, we develop a data-based polytopic representation for the MAS. We tackle the issue of infeasibility in the set of output regulator equations caused by the noise by seeking approximate solutions via constrained fitting error minimization. This method utilizes measured data and a noise-matrix polytope to ensure near-optimal output synchronization. Stability conditions in the form of data-dependent semidefinite programs are derived, providing stabilizing controller gains for each follower. The proposed distributed data-driven control protocol achieves near-optimal output synchronization by ensuring the convergence of the tracking error to a bounded polytope, with the polytope size positively correlated with the noise bound. Numerical tests validate the practical merits of the proposed data-driven design and theory. © 2023, CC BY.

关键词： Multi agent systems

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Cr(Vi) Secondary Pollution in Cr(Iii)-Containing Waste Residue Triggered by Sulfite Under Visible Light Irradiation: The Formation of •Oh and Fe(V)

SSRN

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SSRN 2022年

作者： Lei, Dashi Gui, Wanting Zhang, Shuai Peng, Xiangyu Xiao, Wei Zhang, Jing Xue, Juanqin Wang, Yubin School of Resources Engineering Xi’an University of Architecture and Technology Xi’an710055 China Department of Chemical Engineering Xi’an University of Architecture and Technology Xi’an710055 China National Engineering Laboratory for VOCs Pollution Control Materials & Technology Research Center for Environmental Material and Pollution Control Technology University of Chinese Academy of Sciences Beijing101408 China

The reduced chromite ore processing residue (rCOPR) deposited in environments causes reoccurrence of Cr(VI), resulting in serious secondary pollution. In this work, we studied the release and oxidation behaviors of Cr(III)–Fe(III) hydroxide under visible light, in presence of sulfite, the common reductant in rCOPR. At acidic conditions, only Cr(III) released from CrxFe1−x(OH)3 under light, while a large amount of Cr(VI) was produced after adding sulfite. At basic pH of 9, there is still 1.71 μM Cr(VI) released into environment. Radicals fitting results reveal that sulfite triggers radical chain reactions and promotes hydroxyl radical (•OH) production, which is responsible for Cr(III) oxidation and dissolution at acidic conditions. XANES and HPLC-MS analysis demonstrate the formation of Fe(V) on solid surface, which leads to the oxidation of Cr(III) into Cr(VI) through non-radical mechanism. Moreover, coarsening grain size was proposed to enhance solid stability, and Cr(VI) release efficiency significantly decreased 93.57%. © 2022, The Authors. All rights reserved.

关键词： Oxidation

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Approaching the Influence of Marine Environment Degradation on Impacted Carbon Fiber/Vinyl Ester Laminate with Electronic Speckle Pattern Interferometry Investigation

Approaching the Influence of Marine Environment Degradation ...

引用

International Symposium on Dynamic Response and Failure of Composite Materials, DRAF 2022

作者： Ilaria, Papa Valentina, Lopresto Pietro, Russo Vito, Pagliarulo Department of Chemical Materials and Production Engineering University of Naples Federico II Piazzale V. Tecchio 80 Napoli80125 Italy CNR National Research Council IPCB Institute for Polymers Composites and Biomaterials Via Campi Flegrei 34 NA Pozzuoli80078 Italy CNR National Research Council ISASI Institute of Applied Sciences and Intelligent Systems Via Campi Flegrei 34 NA Pozzuoli80078 Italy

ISBN: (纸本)9783031285462

This work considered the influence of water absorption aging on the impact damage of wet carbon fiber/vinyl ester laminate. In detail, low-velocity impact tests were systematically performed before and after conditioning the composite in dry and water-saturated states in a saline environment at 3%, respectively. In addition, Electronic Speckle Pattern Interferometry analyzed the damage of the impacted specimens, and the results were compared to the absorbed energy according to three impact energy levels: 10J, 20J, and 30J. © 2023, The Author(s), under exclusive license to Springer Nature Switzerland AG.

关键词： Carbon fibers

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Fabrication of translucent graded dental crown using zirconia-yttrium multi-slurry tape casting 3D printer

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Journal of the Mechanical Behavior of Biomedical Materials 2024年 152卷 106406页

作者： Romario, Yulius Shan Bhat, Chinmai Ramezani, Maziar Pasang, Tim Chen, Zhangwei Jiang, Cho-Pei Graduate Institute of Manufacturing Technology National Taipei University of Technology 1 Sec. 3 Zhongxiao E. Rd. Taipei10608 Taiwan High-value Biomaterials Research and Commercialization Center National Taipei University of Technology 1 Sec. 3 Zhongxiao E. Rd. Taipei10608 Taiwan Department of Mechanical Engineering Auckland University of Technology Auckland1010 New Zealand Department of Engineering Design Manufacturing and Management Systems Western Michigan University KalamazooMI4900-5200 United States Additive Manufacturing Institute Shenzhen University Shenzhen518060 China Guangdong Key Laboratory of Electromagnetic Control and Intelligent Robotics College of Mechatronics and Control Engineering Shenzhen University Shenzhen518060 China Department of Mechanical Engineering National Taipei University of Technology 1 Sec. 3 Zhongxiao E. Rd. Taipei10608 Taiwan

This paper aims to fabricate functionally graded dental crown using a multi-slurry tape casting additive manufacturing technology. The different luminescence of the dental crown was obtained with different composition of zirconia and yttria. Zirconia with tunable mechanical properties and translucency are obtained by adding 3, 3.5, 4, 4.5, and 5 mol% of yttrium oxide to zirconia powder. After obtaining the printable slurry with maximum solid loading, the green bodies are prepared using the in-house built high-speed multi-ceramic tape casting technology. They are later sintered with two-stage sintering method. After the successful fabrication, the mechanical properties and translucency of the specimens were evaluated with Vickers hardness, three-point bending and translucency parameter tests. Finally, an FGM tooth crown with five photocurable slurries is proposed to demonstrate the translucent gradient effect of sintered part. The solid loading of 80% zirconia and 20% resin delivered samples without any surface cracks. The shrinkage ratio analysis showed that the sintered sample dimension was reduced by 20%, 20%, and 23% along X, Y, and Z directions. The samples fabricated with 3% yttrium oxide to zirconia delivered excellent hardness (1687 HV) and flexural strength (650.6 MPa). However, the relative luminescence increased with increasing the yttrium oxide for 3–5 mol%. With the optimized process parameters, the proposed dental crown is fabricated and analyzed for their shrinkage ratio, mechanical, and translucency properties. The study proposes the potential of fabricating customized dental crown with gradient translucent appearance. © 2024

关键词： Zirconia

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Power control of Cascaded Doubly Fed Induction Generator Integrated Into Wind Power Generation System Using Sliding Mode control

Power Control of Cascaded Doubly Fed Induction Generator Int...

引用

Renewable Solutions for Ecosystems: Towards a Sustainable Energy Transition (ICRSEtoSET), International Conference on

作者： Ali Mazari Kouider Laroussi Amar Maafa Hamou Ait Abbas Abdelah Kouzou Billel Naceri Laboratory of The Applied and Automation and Industrial Diagnostic University Of Djelfa Djelfa Algeria Department of Electrical Engineering University Of Bouira Bouira Algeria Laboratory of Electrical and Automatic Systems Engineering (LGSEA) University of Bouira Bouira Algeria Laboratory of Identification Commande Control and Communication (LI3CUB) University of Biskra Biskra Algeria

ISBN: (数字)9798350346336

ISBN: (纸本)9798350346343

This paper aims to study the performance of combining two doubly fed induction generators (DFIG) without changing their design, therefore the benefit of eliminating the brushes. The sliding mode control (SMC) is used for controlling the active and reactive powers for the cascaded doubly fed induction generator (CDFIG) integrated into wind power generation system (WPGS). Additionally, the system incorporates a maximum power point tracking strategy (MPPT) to optimize energy generation and a pitch control mechanism for the wind turbine's blades to adjust to changing wind conditions.

关键词： Maximum power point trackers Wind energy generation Brushes Uncertainty Wind speed Power control Doubly fed induction generators

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Reduced Graphene Oxide Reinforced Pda-Gly-Pva Composite Hydrogel as Strain Sensors with High Conductivity and Sensitivity for Monitoring Human Motion

SSRN

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SSRN 2023年

作者： Ke, Xiaoling Mu, Xiaojiang Chen, Siyi Zhang, Zhixiang Zhou, Jianhua Chen, Yulian Gao, Jie Liu, Jing Wang, Xiaoyang Ma, Chuanguo Miaoa, Lei Guangxi Key Laboratory of Information Materials Engineering Research Center of Electronic Information Materials and Devices Ministry of Education School of Materials Science and Engineering Guilin University of Electronic Technology Guilin541004 China College of Arts and Science University of Tokyo Tokyo1538902 Japan Department of Chemical Systems Engineering Graduate School of Engineering Nagoya University Nagoya464-8603 Japan Materials Science and Engineering Guilin University of Electronic Technology No.1 Jinji Road Qixing District Guilin541004 China

Hydrogels possessing soft skin-friendly properties and high biocompatibility are found to be a promising alternative to traditional sensors. However, it is still a great challenge to balance the electrical conductivity and the sensitivity. The sensitivity-improved strain sensor was designed via reduced graphene oxide (rGO) reinforced polydopamine (PDA)-glycerol (Gly)-polyvinyl alcohol (PVA) composite hydrogels (PGHs). The hydrogels exhibit excellent sensing sensitivity with the gauge factor of 2.78, conductivity up to 2.2 S/m, tensile deformation of 200%, fast response time of 370ms and recovery time of 260ms, surpassing majority of previously reported hydrogel-based strain sensors. This improvement can be attributed to the high electrical conductivity and uniform distribution of rGO assited with Gly and PDA. It also exhibits attractive monitoring effect for the hand movements and precise detection feedback for the slight dynamics of the pharynx. The exploitation of hydrogel-based strain sensors is demonstrated as a potential sustainable solution to dynamic detection and communication. © 2023, The Authors. All rights reserved.

关键词： Hydrogels

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