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Quantification of membrane protein diffusion on endothelial cell surfaces under shear stress using a photochromic fluorescent protei

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Journal of Biorheology 2024年第2期38卷 88-93页

作者： Kawasaki, Naoto Hamasaki, Keita Sasaki, Saori Takeishi, Naoki Kudo, Susumu Graduate School of Engineering of Functional Control Systems Shibaura Institute of Technology 3-7-5 Toyosu Tokyo Koto-ku135-8548 Japan Department of Applied Chemistry Shibaura Institute of Technology 3-7-5 Toyosu Tokyo Koto-ku135-8548 Japan Department of Mechanical Engineering Kyushu University 744 Motooka Nishi-ku Fukuoka819-0395 Japan

The fluidity of endothelial cell (EC) membranes, consisting of a lipid bilayer and heterogeneous multicom-ponent, undergoes alterations in response to shear stress. Although localized variations in membrane fluidity are assumed to induce region-specific signal transduction by modulating membrane protein dynamics on almost planar bilayers, membrane protein diffusion under shear stress remains uncertain. Hence, this study aimed to quantify membrane protein diffusion on ECs under fluid shear stress. We used the photochromic fluorescent protein Dronpa to tag a glycosylphosphatidylinositol-anchored protein (GPI-AP), which diffuses across the outer membranes, and quantified its surface diffusion based on the spatiotemporal distribution of Dronpa-Green-labeled GPI-AP (DGGPI-AP) on EC membranes. We developed an experimental platform to meas-ure the GPI-AP surface diffusion under fluid shear stress and quantified the diffusion coefficient of GPI-AP in two distinct membrane regions: upstream and downstream relative to the direction of fluid flow. Our experimental results showed that there were not statistically significant differ-ences in GPI-AP diffusion on EC membranes between the time points or between the upstream and downstream regions of ECs for at least 15 minutes under shear stress. Our developed methodology and experimental results will be useful to understand a relationship between the membrane protein diffusion and shear-induced cellular processes. © Japanese Society of Biorheology 2024.

关键词： Shear stress

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Robust Consensus Tracking control of Uncertain Multi-Agent systems With Local Disturbance Rejection

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IEEE/CAA Journal of Automatica Sinica 2023年第2期10卷 427-438页

作者： Pan Yu Kang-Zhi Liu Xudong Liu Xiaoli Li Min Wu Jinhua She the Faculty of Information Technology and the Beijing Institute of Artificial Intelligence Beijing University of TechnologyBeijing 100124China IEEE the Department of Electrical and Electronic Engineering Chiba UniversityChiba 263-8522Japan the Beijing Aerospace Automatic Control Institute Beijing 100854China the School of Automation China University of GeosciencesWuhan 430074China the School of Engineering Tokyo University of TechnologyHachiojiTokyo 192-0982Japan

In this paper,a new distributed consensus tracking protocol incorporating local disturbance rejection is devised for a multi-agent system with heterogeneous dynamic uncertainties and disturbances over a directed *** is of two-degree-of-freedom ***,a robust distributed controller is designed for consensus tracking,while a local disturbance estimator is designed for each agent without requiring the input channel information of *** condition for asymptotic disturbance rejection is ***,even when the disturbance model is not exactly known,the developed method also provides good disturbance-rejection ***,a robust stabilization condition with less conservativeness is derived for the whole multi-agent ***,a design algorithm is ***,comparisons with the conventional one-degree-of-freedombased distributed disturbance-rejection method for mismatched disturbances and the distributed extended-state observer for matched disturbances validate the developed method.

关键词： Directed graph distributed control disturbance rejection dynamic uncertainties multi-agent systems robust control

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Composite Model Predictive control for PMSM Servo systems Based on Backlash Cancellation and Disturbance Compensation 41

Composite Model Predictive Control for PMSM Servo Systems Ba...

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第41届中国控制会议

作者： Lei Li Zuo Wang Shihua Li School of Automation Southeast University Key Laboratory of Measurement and Control of Complex Systems of Engineering Ministry of Education Shenzhen Research Institute Southeast University

ISBN: (数字)9789887581536

ISBN: (纸本)9781665482561

This paper investigates the speed regulation problem of permanent magnet synchronous motor(PMSM) servo system with backlash based on model predictive control(MPC).It is known that the existing backlash in servo systems causes steady-state errors,limit cycles and rigid *** order to counteract the adverse performance degradations of the backlash nonlinearity,a composite MPC strategy is investigated based on backlash cancellation and disturbance ***,an approximated dead-zone model of backlash is ***,a generalized proportional-integral observer(GPIO) is constructed to estimate the lumped disturbances,including backlash modelling residual error,parameter uncertainties and external *** introducing disturbance estimation and backlash transmission torque into system model,a composite MPC can be obtained based on the prediction-precision enhanced model,which brings significantly improvements to control ***,the efficacy of the presented method is demonstrated by simulation results.

关键词： Model predictive control(MPC) PMSM servo system Backlash cancellation Generalized proportional-integral observer(GPIO)

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Exploring Image Generation for UAV Change Detection

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IEEE/CAA Journal of Automatica Sinica 2022年第6期9卷 1061-1072页

作者： Xuan Li Haibin Duan Yonglin Tian Fei-Yue Wang Peng Cheng Laboratory Shenzhen 518000China IEEE State Key Laboratory of Virtual Reality Technology and Systems School of Automation Science and Electrical EngineeringBeihang UniversityBeijing 100083 Department of Automation University of Science and Technology of ChinaHefei 230027 State Key Laboratory for Management and Control of Complex Systems Institute of AutomationChinese Academy of SciencesBeijing 100190China Research Center for Computational Experiments and Parallel Systems Technology National University of Defense TechnologyChangsha 410073China

Change detection(CD)is becoming indispensable for unmanned aerial vehicles(UAVs),especially in the domain of water landing,rescue and ***,even the most advanced models require large amounts of data for model training and ***,sufficient labeled images with different imaging conditions are *** by computer graphics,we present a cloning method to simulate inland-water scene and collect an auto-labeled simulated *** simulated dataset consists of six challenges to test the effects of dynamic background,weather,and noise on change detection ***,we propose an image translation framework that translates simulated images to synthetic *** framework uses shared parameters(encoder and generator)and 22×22 receptive fields(discriminator)to generate realistic synthetic images as model training *** experimental results indicate that:1)different imaging challenges affect the performance of change detection models;2)compared with simulated images,synthetic images can effectively improve the accuracy of supervised models.

关键词： Change detection computer graphics image translation simulated images synthetic images unmanned aerial vehicles(UAVs)

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Application and Certification of Software Models Used in Development 6

Application and Certification of Software Models Used in Dev...

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6th IEEE International Conference and Workshop Obuda on Electrical and Power engineering, CANDO-EPE 2023

作者： Schuster, Gyorgy Obuda University Budapest Hungary Institute of Electronics and Communication Systems Hungary Kando Kalman Faculty of Electrical Engineering Department of Instrumentation and Automation Hungary

ISBN: (纸本)9798350328752

As the complexity of tasks increases, developers have to use different tools. We talked about this in details in some previous publications. In this paper, we will touch on a special area that is increasingly coming to the fore these days, which is the question of the application of models. However, the application of models in safety-critical systems raises the question of the reliability of the models and the questions of the certification of these software tools. © 2023 IEEE.

关键词： Software reliability

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System Structure for Railway Switch Points and Signals Electrical Interlocking Powered by Renewable Energy Sources and Emergency Cable Network

System Structure for Railway Switch Points and Signals Elect...

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2024 International Conference on Industrial engineering, Applications and Manufacturing, ICIEAM 2024

作者： Efanov, Dmitrii V. Ruziev, Davron Kh. Khóroshev, Valerii V. Transport Higher School Of Mechanical Engineering Material And Transport Institute In Peter The Great St. Petersburg Polytechnic University St. Petersburg Russia Tashkent State Transport University 'Automation And Remote Control' Department Tashkent Uzbekistan Russian University Of Transport 'Automation Remote Control And Communication On Railway Transport' Department Moscow Russia 'Automation Remote Control And Communication On Railway Transport' Department Moscow Russia

ISBN: (纸本)9798350395013

Article propose a hybrid technology of power supply in the electrical interlocking systems development for railway stations. The main feed input is proposed to be carried out from renewable energy sources. Such sources are near the railway track. Redundant power supply should be provided using traditional cable networks with local placement. The article presents the structures of electrical interlocking systems based on hybrid technology. In addition, when supplying power to railway automation and remote control, the power supply must be guaranteed. That is why, at this stage of the transition, it is necessary to maintain redundant cable glands. In the future, a complete transition to energy supply from renewable energy sources is proposed. The authors considered the use of energy sources with solar panels. The following solutions are proposed for organizing the power supply of railway automation and remote-control devices: providing power supply in the yard neck with local power sources - solar panels, linking between wayside device and the signal box using fiber optic cables and radio channels. The main attention is paid to the environmentally friendly energy sources, the wire routing shorts and maintenance. © 2024 IEEE.

关键词： Energy efficiency

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Accelerated Degradation Modeling of Power MOSFET Under Multiple Stresses 12

Accelerated Degradation Modeling of Power MOSFET Under Multi...

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12th International Conference on Quality, Reliability, Risk, Maintenance, and Safety engineering, QR2MSE 2022

作者： Ye, Xuerong Hu, Yifan Feng, Rui Chen, Cen Zhai, Guofu Tang, Loon Ching School of Electrical Engineering and Automation Harbin Institute of Technology Harbin China Shanghai Aerospace Control Technology Institute Shanghai China Department of Industrial and Systems Engineering & Management National University of Singapore Singapore

ISBN: (纸本)9781839538360

Power MOSFET is one of the most critical components in power electronic converters. However, it often performs a lower reliability index under multiple stresses. Conventional single-stress accelerated degradation test (ADT) and corresponding statistical inference are unable to evaluate its reliability accurately. In this article, a new accelerated degradation model is designed by combining multi-stress accelerated model and the generalized Wiener process model. This comprehensive model not only establishes the relationship between drift coefficient and multiple stresses but also take the nonlinearity and uncertainties existing in degradation process into consideration. Subsequently, an ADT with temperature, electrical and vibration stresses for power MOSFET is carried out to obtain the degradation data under different stress levels, which is employed for unknown parameter estimation. Comparative analysis demonstrates that the proposed model can provide a more accurate and reasonable reliability assessment result. © 2022 IEEE.

关键词： Power MOSFET

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Line-of-sight based three-dimensional path following control for an underactuated robotic dolphin

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Science China(Information Sciences) 2021年第1期64卷 215-226页

作者： Jincun LIU Zhenna LIU Junzhi YU State Key Laboratory for Turbulence and Complex System Department of Mechanics and Engineering ScienceBIC-ESATCollege of EngineeringPeking University Department of Electrical and Automation Shandong Labor Vocational and Technical College State Key Laboratory of Management and Control for Complex Systems Institute of AutomationChinese Academy of Sciences

This paper investigates the three-dimensional(3-D) path following control problem for an underactuated robotic dolphin. With a comprehensive consideration of the mechanical constraint and swimming principle of the robotic dolphin, a decoupling motion strategy is proposed to produce yaw and pitch maneuvers simultaneously. Then, kinematics and dynamics models for 3-D dolphin-like swimming are established,followed by simulations of the path following control. Furthermore, a novel lookahead based 3-D line-ofsight(LOS) guidance law is developed and implemented to obtain desired attitude angles with its simplicity,intuitiveness, and small computational footprint. Finally, simulation results illustrate the feasibility and effectiveness of the proposed path following control methods.

关键词： robotic dolphin path following 3-D LOS guidance law decoupling motion

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Unified Addressing Algorithm for Tasks in Cloud control systems 41

Unified Addressing Algorithm for Tasks in Cloud Control Syst...

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第41届中国控制会议

作者： Guan Wang Yufeng Zhan Yuanqing Xia Liping Yan Key Laboratory of Intelligent Control and Decision of Complex Systems School of AutomationBeijing Institute of Technology School of Information Science and Engineering University of ZaoZhuang

ISBN: (数字)9789887581536

ISBN: (纸本)9781665482561

With the rapid development of cloud computing and control theory,a new paradigm of networked control systems named cloud control systems has been proposed to satisfy the requirements of large-scale and complex *** is critical to manage large-scale tasks with personalized needs on the large number of heterogeneous ***,current TCP/IPbased addressing approach does not consider the runtime constraints of tasks,resulting highly concurrent service search,and communication between tasks will become the system *** this paper,we propose a task property encoding method which takes multi-dimensional properties into *** on the proposed encoding method,we design a uniform addressing method for tasks in the ***,we experimentally demonstrate the effectiveness of task property encoding,and the unified addressing method for tasks is more effective than other methods in the case of high task concurrency.

关键词： Cloud control systems address encoding cloud-edge-device cooperation

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Phase chimera states on nonlocal hyperrings

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Physical Review E 2024年第2期109卷 L022201-L022201页

作者： Riccardo Muolo Thierry Njougouo Lucia Valentina Gambuzza Timoteo Carletti Mattia Frasca Department of Systems and Control Engineering Tokyo Institute of Technology Tokyo 152-8552 Japan Department of Mathematics University of Namur B5000 Namur Belgium naXys Namur Institute for Complex Systems University of Namur B5000 Namur Belgium Faculty of Computer Science University of Namur B5000 Namur Belgium Department of Electrical and Electronic Engineering University of Buea P.O. Box 63 Buea Cameroon Department of Electrical Electronics and Computer Science Engineering University of Catania 95125 Catania Italy Istituto di Analisi dei Sistemi ed Informatica “A. Ruberti” IASI-CNR 00185 Roma Italy

Chimera states are dynamical states where regions of synchronous trajectories coexist with incoherent ones. A significant amount of research has been devoted to studying chimera states in systems of identical oscillators, nonlocally coupled through pairwise interactions. Nevertheless, there is increasing evidence, also supported by available data, that complex systems are composed of multiple units experiencing many-body interactions that can be modeled by using higher-order structures beyond the paradigm of classic pairwise networks. In this work we investigate whether phase chimera states appear in this framework, by focusing on a topology solely involving many-body, nonlocal, and nonregular interactions, hereby named nonlocal d-hyperring, (d+1) being the order of the interactions. We present the theory by using the paradigmatic Stuart-Landau oscillators as node dynamics, and we show that phase chimera states emerge in a variety of structures and with different coupling functions. For comparison, we show that, when higher-order interactions are “flattened” to pairwise ones, the chimera behavior is weaker and more elusive.

关键词： Chimera states Collective behavior in networks Dynamics of networks Pattern formation Synchronization Synchronization transition

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