We describe a fiber-optic-based confocal fluorescence spectrometer for obtaining depth-resolved, attenuated fluorescence emission spectra with lateral resolution of several microns and depth resolution of tens of micr...
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We describe a fiber-optic-based confocal fluorescence spectrometer for obtaining depth-resolved, attenuated fluorescence emission spectra with lateral resolution of several microns and depth resolution of tens of microns. The confocal optics of the spectrometer are small, inexpensive, and easy to construct and to interface to existing spectrometers. We compare the performance of this system to that of conventional fluorescence spectrometers for nonscattering homogeneous and inhomogeneous samples. We demonstrate that the confocal measurements readily provide information about the sample geometry and optical properties not available from nonconfocal measurements. Potential applications of the technique are discussed.
A new technique for modelling biological signals as a linear combination of non-orthogonal Gabor logons is described. The technique has been applied to two types of signals, Event-Related Potentials (ERPs) and temporo...
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Presents a new technique for reconstruction and imaging of volumetric neuromuscular activity using digital signal processing of multichannel surface potential recordings. Magnetic resonance imaging is used to chart an...
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Presents a new technique for reconstruction and imaging of volumetric neuromuscular activity using digital signal processing of multichannel surface potential recordings. Magnetic resonance imaging is used to chart anatomical structures and quantify material permittivity within the inhomogenous volume. Array signal processing is used localize neuromuscular action potentials based on the available anatomical and electromyographic measurements. Poisson's equation is then solved using finite element methods coupled with signal processing estimation techniques. This new imaging tool can greatly enhance clinical diagnosis and treatment of neuromuscular injuries and disorders by localizing pathological signals within muscle.< >
Multi-element synthetic aperture imaging methods suitable for applications with severe cost and size limitations are explored. Array apertures are synthesized using an active multi-element receive subaperture and a mu...
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Multi-element synthetic aperture imaging methods suitable for applications with severe cost and size limitations are explored. Array apertures are synthesized using an active multi-element receive subaperture and a multi-element defocused transmit subaperture. The performance of multi-element methods has been tested and compared to conventional imagers through measurements with a 3.5 MHz, 128-element transducer array on different gel phantoms. The multi-element synthetic aperture methods achieve higher electronic signal to noise ratio and better contrast resolution than conventional synthetic aperture techniques, approaching conventional phased array performance
A new technique for modelling biological signals as a linear combination of non-orthogonal Gabor logons is described. The technique has been applied to two types of signals, event-related potentials (ERPs) and temporo...
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A new technique for modelling biological signals as a linear combination of non-orthogonal Gabor logons is described. The technique has been applied to two types of signals, event-related potentials (ERPs) and temporomandibular joint (TMJ) clicks. Examination of time-frequency representations of these signals revealed that they appear to consist of a small number of localized energy concentrations. Attempts to capture this apparent low dimensionality with the standard orthogonal Gabor expansion and the standard wavelet transform were unsuccessful. However, the non-orthogonal Gabor decomposition method described in this paper provides a compact, accurate signal representation and the parameters provide a good basis for ERP category and TMJ click classification.< >
A thorough understanding of fundamental microwave absorption mechanisms in ionic crystalline solids is important for microwave sintering of ceramics, as well as the design of high speed electronic packaging, advanced ...
A thorough understanding of fundamental microwave absorption mechanisms in ionic crystalline solids is important for microwave sintering of ceramics, as well as the design of high speed electronic packaging, advanced radomes, etc. Of particular importance to these applications are how the density and type of crystalline defects affect the dominant microwave absorption mechanisms. We have designed experiments to measure microwave absorption in NaCl samples with controlled variations in defect conditions(pure, point defects, dislocations and grain boundaries) at different temperatures (20-400 °C) and frequencies (2-20 GHz). Initial results are reported and discussed.
An optical scattering method, which measures the size, number, and type of microemboli located in flowing whole blood, is described. A perturbation solution to the one speed radiative transport equation is used to mod...
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An optical scattering method, which measures the size, number, and type of microemboli located in flowing whole blood, is described. A perturbation solution to the one speed radiative transport equation is used to model the scattering geometry. The approximate two-parameter phase function is used to investigate scattering characteristics of microemboli. The nature of microemboli is determined by comparing the scattered intensity ratio in two forward directions, while the size and number are determined by considering intensities scattered perpendicularly to the incident direction. This method has been tested with several sizes of polystyrene spheres, air bubbles, and blood dots. The results showed excellent agreement with calculations.
This paper presents a comparison of H/sub /spl infin////spl mu/-synthesis sliding-mode that was done for heading and depth control of the Draper Laboratory/MIT Sea Grant Sea Squirt autonomous underwater vehicle (AUV)....
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This paper presents a comparison of H/sub /spl infin////spl mu/-synthesis sliding-mode that was done for heading and depth control of the Draper Laboratory/MIT Sea Grant Sea Squirt autonomous underwater vehicle (AUV). The approximate decoupling of the AUV heading and depth dynamics allowed us to have decoupled heading and depth designs for both the H/sub /spl infin////spl mu/-synthesis and the sliding-mode methodologies. The H/sub /spl infin////spl mu/-synthesis based controllers and the sliding-mode based controllers were implemented and tested by using a simulation of the AUV full order nonlinear model (including thruster and sensor dynamics). We analyzed the performance of the controllers (for a simultaneous depth and maneuver) over the approximate range of axial velocities commanded by the AUV. The performance of the controllers in the face of additive sensor noise was also analyzed.
The technique of serial optical sectioning by laser scanning confocal microscopy (LSCM), in conjunction with off-line digital image analysis, was used to quantize the morphological changes occurring during angiogenesi...
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The technique of serial optical sectioning by laser scanning confocal microscopy (LSCM), in conjunction with off-line digital image analysis, was used to quantize the morphological changes occurring during angiogenesis and revascularization of pancreatic islets transplanted at the renal subcapsular site in rats. The process consisted of in-vivo imaging of the microvasculature which was optically enhanced by the administration of a fluorescent probe into the circulating blood. Serial two-dimensional (2-D) optical sections were obtained through the vascular bed at varying z-intervals in order to perform a computer reconstruction of the complete three-dimensional (3-D) morphology. Image processing algorithms such as gray level thresholding, median filtering, skeletonization, region labeling and template matching were applied to compute the vessel density, lengths and diameters of the neovasculature, and the tortuosity index.< >
The authors address the analysis of three dimensional shape and shape change in nonrigid biological objects imaged via a stereo light microscope (SLM). Most existing stereo or motion analysis techniques cannot be appl...
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The authors address the analysis of three dimensional shape and shape change in nonrigid biological objects imaged via a stereo light microscope (SLM). Most existing stereo or motion analysis techniques cannot be applied to microscopic biological images because they usually lack salient features. The authors propose an integrated approach for the reconstruction of 3D structures and motion analysis for scenes where only a few informative features are available. The key components of this framework are: (1) image registration, (2) region-of-interest extraction, and (3) stereo and motion analysis using a cooperative spatial and temporal matching process. The authors describe these three stages of processing and illustrate the efficacy of the proposed approach using real images of a live frog's ventricle. The reconstructed dynamic 3-D structures of the ventricle are demonstrated in the authors' experimental results.< >
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