In Wireless Multimedia Sensor Networks(WMSNs),nodes capable of retrieving video,audio,images,and small scale sensor data,tend to generate immense traffic of various *** energy-efficient transmission of such a vast amo...
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In Wireless Multimedia Sensor Networks(WMSNs),nodes capable of retrieving video,audio,images,and small scale sensor data,tend to generate immense traffic of various *** energy-efficient transmission of such a vast amount of heterogeneous multimedia content while simultaneously ensuring the quality of service and optimal energy consumption is ***,we propose a Power-Efficient Wireless Multimedia of Things(PE-WMoT),a robust and energy-efficient cluster-based mechanism to improve the overall lifetime of *** a PE-WMoT,nodes declare themselves Cluster Heads(CHs)based on available *** cluster formation and CH declaration processes are completed,the Sub-Cluster(SC)formation process triggers,in which application base nodes within close vicinity of each other organize themselves under the administration of a Sub-Cluster Head(SCH).The SCH gathers data from member nodes,removes redundancies,and forwards miniaturized data to its respective ***-WMoT adopts a fuzzy-based technique named the analytical hierarchical process,which enables CHs to select an optimal SCH among available SCs.A PE-WMoT also devises a robust scheduling mechanism between CH,SCH,and child nodes to enable collision-free data *** results revealed that a PE-WMoT significantly reduces the number of redundant packet transmissions,improves energy consumption of the network,and effectively increases network throughput.
Food and waterborne diseases pose considerable public health threats even in highly industrialized parts of the *** of these pathogens in food can be Escherichia coli O157:H7,Salmonella sp.,and Listeria ***,reliable d...
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Food and waterborne diseases pose considerable public health threats even in highly industrialized parts of the *** of these pathogens in food can be Escherichia coli O157:H7,Salmonella sp.,and Listeria ***,reliable detection of pathogens mitigates serious health problems and economic losses due to outbreaks and robust tests safeguard the food *** this study,a smartphone-based apparatus was employed to demonstrate quantitative detection of *** validate the applicability of the present smartphone-based fluorescence device,RNA was extracted from the *** K-12 strain and amplified using two different primers(dnaK and rpoA)via quantitative polymerase chain reaction(qPCR).Serial dilutions of RNA from 10 to 0.0001 ng/μL were prepared at the start of the PCR amplification and the PCR products were detected by CYBR Green1-based *** a proof-of-concept test for the smartphone system,samples from these PCR products were then *** detection system employed a novel algorithm to analyze fluorescence signals and read changes in *** DNA *** correlations between the fluorescence percentage and DNA concentrations were R=0.945 for the dnaK primer and R=0.893 for the rpoA primer,*** this new fluorescent analysis technique resulted in comparable accuracy to the real-time PCR fluorescent signal *** key innovation of this approach was to combine efficient image processing encoded into a smartphone application with a low-cost 3-D printed device that allowed quantification of bacterial nucleic acid.
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