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Denaturation methods for reusable magnetic biosensors

Lab on a chip 2025年 2025 May 29页

作者： Suhyeon Park Songeun Kim Shan X Wang Jung-Rok Lee Department of Mechanical and Biomedical Engineering Ewha Womans University Seoul 03760 Republic of Korea. Graduate Program in Smart Factory Ewha Womans University Seoul 03760 Republic of Korea. jungrok@ewha.ac.kr. Department of Materials Science and Engineering Stanford University Stanford California 94305 USA. Department of Electrical Engineering Stanford University Stanford California 94305 USA.

Nanoscale biosensors for sensitive DNA detection require advanced and precise fabrication techniques, which make them highly expensive and result in low yield rates. For such DNA biosensors, sensor regeneration is highly desirable. In this study, we investigated the effectiveness of various denaturants, including ultrapure water, urea solution, tris-ethylenediaminetetraacetic acid buffer, and dimethyl sulfoxide (DMSO), for the denaturation of target DNAs hybridized to probe DNAs on sensors. We used giant magnetoresistive (GMR) biosensors equipped with a temperature control unit in conjunction with magnetic nanoparticles. To examine the effect of DNA sequence on denaturation efficiency, 14 orthogonal DNA pairs were designed and tested. Furthermore, to maintain a consistent sensitivity in subsequent measurements, we evaluated the integrity of the probe DNAs on the sensors after denaturation. Among all the denaturants tested, 40% DMSO demonstrated excellent performance in the denaturation of probe DNAs covalently bonded to the sensors, without any heating process. This optimal denaturant can be applied to other planar DNA biosensor systems; moreover, GMR biosensors can facilitate the evaluation of newly developed denaturants.

关键词： Magnetic nanoparticles

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Spatiotemporal T cell dynamics in a 3D bioprinted immunotherapy model

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Bioprinting 2022年 28卷

作者： Morley, Cameron D. Flores, Catherine T. Drake, Jeffery A. Moore, Ginger L. Mitchell, Duane A. Angelini, Thomas E. University of Florida Herbert Wertheim College of Engineering Department of Mechanical and Aerospace Engineering Gainesville 32611 FL United States University of Florida Brain Tumor Immunotherapy Program Preston A. Wells Jr. Center for Brain Tumor Therapy Lillian S. Wells Department of Neurosurgery University of Florida 1149S Newell Dr L2-100 Gainesville 32611 FL United States University of Florida Herbert Wertheim College of Engineering Department of Materials Science and Engineering Gainesville 32611 FL United States University of Florida Herbert Wertheim College of Engineering J. Crayton Pruitt Family Department of Biomedical Engineering Gainesville 32611 FL United States

3D bioprinting-focused research is often driven by the aspiration to manufacture functional tissues and organs for implantation, yet decades of additional research is likely needed to develop such technologies, broadly. By contrast, we currently have 3D bioprinting tools capable of generating precise, spatially defined distributions of different cell types that can be used in fundamental and applied research, without delay. Here, we demonstrate this capability by 3D printing adoptive immunotherapy models using KR158B cells (murine glioma), hematopoietic stem cells (HSCs), and tumor-reactive T cells. We leverage a recently developed material made from packed microgel particles that serves simultaneously as a 3D printing support material and a 3D culture medium. With this approach we create well-defined 3D cell distributions and investigate the interactions between the different cell populations with time-lapse confocal microscopy. We find that 3D printed tumor spheroids containing HSCs recruit T cells more rapidly than those lacking HSCs, where T cell motion appears to be guided by diffusing cytokines originating at the tumor spheroid surface. After the T cells interact with the glioma structures, we collect the different cell populations by running our 3D bioprinter in reverse and perform transcriptomic analysis. We find that the differences in gene expression, comparing tumor spheroids printed with and without HSCs, are consistent with those found in a murine model. These results establish a path forward for developing validated 3D printed models for pre-clinical testing. © 2022 Elsevier B.V.

关键词： Adoptive Cell Transfer (ACT) Bioprinting Cell migration Microgel

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Tribological properties of hydroxyapatite-coated nanorods on Ti-6Al-4V surfaces

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Scientific reports 2025年第1期15卷 19113页

作者： Sarah Akua Osafo Tabiri Asumadu Desmond Klenam Precious Etinosa John David Obayemi Benjamin Agyei-Tuffour Abu Yaya David Dodoo-Arhin Stanley Chijioke Eluu Wole Soboyejo Department of Materials Science and Engineering School of Engineering Sciences College of Basic and Applied Sciences University of Ghana Legon Accra Ghana. Department of Biomaterial Sciences Dental School University of Ghana Korle Bu Campus Accra Ghana. Department of Mechanical Engineering Program in Materials Science and Engineering Worcester Polytechnic Institute 100 Institute Road Worcester MA 01609 USA. Department of Materials Engineering Sunyani Technical University Sunyani Ghana. Global Center of Advanced Materials and Manufacturing Department of Mechanical Engineering College of Engineering State University of New York (SUNY) Polytechnic Institute 100 Seymour Street Utica NY 13502 USA. Next Frontiers in Advanced Material Laboratory School of Chemical and Metallurgical Engineering University of the Witwatersrand 1 Jan Smuts Avenue Johannesburg 2050 South Africa. Department of Biomedical Engineering Worcester Polytechnic Institute Gateway Park Life Sciences and Bioengineering Centre 60 Prescott Street Worcester MA 01609 USA. Department of Biotechnology Ebonyi State University Abakaliki Nigeria. Department of Pharmaceutical Microbiology and Biotechnology Faculty of Pharmaceutical Sciences Nnamdi Azikiwe University Awka Anambra State Nigeria. Department of Mechanical Engineering Program in Materials Science and Engineering Worcester Polytechnic Institute 100 Institute Road Worcester MA 01609 USA. soboyew@sunypoly.edu. Global Center of Advanced Materials and Manufacturing Department of Mechanical Engineering College of Engineering State University of New York (SUNY) Polytechnic Institute 100 Seymour Street Utica NY 13502 USA. soboyew@sunypoly.edu. Department of Biomedical Engineering Worcester Polytechnic Institute Gateway Park Life Sciences and Bioengineering Centre 60 Prescott Street Worcester MA 01609 USA. soboyew@sunypoly.edu.

This paper presents the tribological properties and bioactivity of nanostructured hydroxyapatite (HA) from biowaste sources and coated onto Ti-6Al-4V substrates using a novel pack cementation method. The process introduced HA pillars/nanorods on the surfaces of Ti-6Al-4V to enhance their osseointegration for dental implants. The mechanical and tribological properties were studied with nanoindentation, and pin-on-disk techniques, following the microstructural characterization of the coatings with atomic force microscopy, X-ray diffraction, Raman spectroscopy, scanning electron microscopy, and energy dispersive x-ray spectroscopy. The study also examines the surface bioactivity and elucidates the underlying friction and wear mechanisms of the HA-coated and annealed Ti-6Al-4V surface. The study results show a bone bonding capacity of the biowaste-derived HA-coated substrate with improved hardness and tribological properties. The implications of the study are discussed for the development of nano-structured HA-coated Ti-6Al-4V for dental implants with improved osseointegration for dental and biomedical applications.

关键词： Bioactivity Dental implants Osseointegration Surface modification Titanium alloys Wear resistance

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Evaluation of mechanical properties, biodegradability and bioactivity of forsteritediopside scaffolds coated by polycaprolacton fumarate

Evaluation of mechanical properties, biodegradability and bi...

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materials science and Technology 2019, MS and T 2019

作者： Sadeghzade, Sorour Emadi, Rahmatollah Tavangarian, Fariborz Materials research Group Department of Materials engineering Isfahan University of Technology Isfahan84156-83111 Iran Mechanical Engineering Program School of Science Engineering and Technology Pennsylvania State University HarrisburgPA17057 United States

ISBN: (纸本)0873397703

One of the significant challenges in bone tissue engineering is the fabrication of highly porous scaffolds with interconnected pores and appropriate mechanical properties. Commonly available synthetic scaffolds are made of ceramic or polymer, but a better combination of properties can be achieved with a composite or multi-component structure. The aim of this study is to produce three-dimensional (forsterite-diopside) porous scaffolds using space holder technique in order to use in bone tissue engineering. Therefore, forsterite and Diopside nanopowders were synthesized via mechanical activation and sol-gel methods, respectively, and then were mixed with various ratios. After addition of sodium chloride as spacer with appropriate weight percentage, the powder mixture was compacted under the uniaxial compression. In order to remove sodium chloride and develop a porous structure, samples were sintered. After determination of the optimal scaffolds in terms of mechanical properties, the scaffolds were coated with 6 wt.% poly caprolactone fumarate solution and their mechanical, physical and biological properties was evaluated. The results showed that the addition of 10 wt.% diopside nanopowder altered the properties of the scaffolds such that compressive strength increased to 4.36 MPa compared to pure forsterite (3.45 MPa). Applying the polymer coating with cross-linked structure on the optimal scaffolds improved the compressive strength by 23%. Based on the results of Archimedes' principle and SEM micrographs, the addition of 10 wt.% diopside had a negligible effect on the porosity but improved the pores morphology. SEM micrographs of scaffolds after soaking in SBF depicted tiny agglomerated bone-like apatite particles. Considering the results obtained, it seems that, the produced scaffolds could be a good candidate for bone tissue engineering applications. Copyright © 2019 MS&T19®

关键词： Porosity

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Evaluation of bioactivity and mechanical properties of silica-based ceramic for using in tissue engineering application

Evaluation of bioactivity and mechanical properties of silic...

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materials science and Technology 2019, MS and T 2019

作者： Tavangarian, Fariborz Sadeghzade, Sorour Emadi, Rahmatollah Mechanical Engineering Program School of Science Engineering and Technology Pennsylvania State University HarrisburgPA17057 United States Materials research Group Department of Materials engineering Isfahan University of Technology Isfahan84156-83111 Iran

ISBN: (纸本)0873397703

Bone engineering presents an alternative approach to repair and regenerate a damaged tissue. For bone repair and bone tissue engineering applications, bioactive materials with different degradation and mechanical properties are needed. Recently hardystonite bioceramic have received great attention for treatment of bone defects. In this study, a bioactive hardystonite (HT) - forstrite (FO) nanocomposite was fabricated by two step sintering method. The structure, morphology and bioactivity potential of the nanocomposite were examined using transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). The composite with optimized mechanical properties was HT-20wt.%Fo that had the compressive modulus and strength of 287 MPa and 149 MPa, respectively. The feasibility of the produced composite for bone tissue engineering application was evaluated using simulated body fluid (SBF). A range of characterization techniques was applied to confirm the deposition of Hydroxyl carbonated apatite (HCA) deposition on the surface of HT-20wt.%Fo composite following 7 days in SBF. Overall, results suggest that HT-20wt.%Fo composite with improved mechanical properties, and apatite formation ability can be a promising candidate for bone tissue engineering applications. Copyright © 2019 MS&T19®

关键词： Nanocomposites

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Correction to: Nucleation of voids at Li‑metal–ceramic–electrolyte interfaces

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MRS Communications 2021年第5期11卷 699-699页

作者： Raj, Rishi Materials Science and Engineering Program Department of Mechanical Engineering University of Colorado Boulder Boulder USA

This article was updated to correct the fourth paragraph in the introduction. Due to errors introduced during the production process Ω appeared as W throughout the paragraph.

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Coordination corrected ab initio formation enthalpies

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npj Computational materials 2019年第1期5卷 633-644页

作者： Rico Friedrich Demet Usanmaz Corey Oses Andrew Supka Marco Fornari Marco Buongiorno Nardelli Cormac Toher Stefano Curtarolo Department of Mechanical Engineering and Materials Science Duke UniversityDurhamNC 27708USA Center for Materials Genomics Duke UniversityDurhamNC 27708USA Department of Physics and Science of Advanced Materials Program Central Michigan UniversityMount PleasantMI 48859USA Department of Physics and Department of Chemistry University of North TexasDentonTX 76203USA Materials Science Electrical EngineeringPhysics and ChemistryDuke UniversityDurhamNC 27708USA Fritz-Haber-Institut der Max-Planck-Gesellschaft 14195 Berlin-DahlemGermany

The correct calculation of formation enthalpy is one of the enablers of ab-initio computational materials *** several classes of systems(***)standard density functional theory produces incorrect *** we propose the“coordination corrected enthalpies”method(CCE),based on the number of nearest neighbor cation–anion bonds,and also capable of correcting relative stability of *** uses calculations employing the Perdew,Burke and Ernzerhof(PBE),local density approximation(LDA)and strongly constrained and appropriately normed(SCAN)exchange correlation functionals,in conjunction with a quasiharmonic Debye model to treat zero-point vibrational and thermal *** benchmark,performed on binary and ternary oxides(halides),shows very accurate room temperature results for all functionals,with the smallest mean absolute error of 27(24)meV/atom obtained with *** zero-point vibrational and thermal contributions to the formation enthalpies are small and with different signs—largely canceling each other.

关键词： corrected vibrational oxides

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Interdependence of Support Wettability - Electrodeposition Rate- Sodium Metal Anode and SEI Microstructure

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Angewandte Chemie 2024年第8期137卷

作者： Chang-An Lo Yixian Wang Varun R. Kankanallu Aditya Singla Dean Yen Xiaoyin Zheng Kaustubh G. Naik Bairav S. Vishnugopi Callum Campbell Vikalp Raj Chonghang Zhao Lu Ma Jianming Bai Feipeng Yang Ruipeng Li Mingyuan Ge John Watt Partha P. Mukherjee David Mitlin Yu-chen Karen Chen-Wiegart Department of Materials Science and Chemical Engineering Stony Brook University Stony Brook NY 11794 USA Materials Science and Engineering Program & Texas Materials Institute The University of Texas at Austin Austin TX 78712 USA School of Mechanical Engineering Purdue University West Lafayette IN 47907 USA National Synchrotron Light Source II Brookhaven National Laboratory Upton NY 11973 USA Center for Integrated Nanotechnologies Los Alamos National Laboratory Los Alamos NM 87545 USA

This study examines how current collector support chemistry (sodiophilic intermetallic Na 2 Te vs. sodiophobic baseline Cu) and electrodeposition rate affect microstructure of sodium metal and its solid electrolyte interphase (SEI). Capacity and current (6 mAh cm −2 , 0.5–3 mA cm −2 ) representative of commercially relevant mass loading in anode-free sodium metal battery (AF-SMBs) are analyzed. Synchrotron X-ray nanotomography and grazing-incidence wide-angle X-ray scattering (GIWAXS) are combined with cryogenic ion beam (cryo-FIB) microscopy. Highlighted are major differences in film morphology, internal porosity, and crystallographic preferred orientation e.g. (110) vs. (100) and (211) with support and deposition rate. Within the SEI, sodium fluoride (NaF) is more prevalent with Te−Cu versus sodium hydride (NaH) and sodium hydroxide (NaOH) with baseline Cu. Due to competitive grain growth the preferred orientation of sodium crystallites depends on film thickness. Mesoscale modeling delineates the role of SEI (ionic conductivity, morphology) on electrodeposit growth and onset of electrochemical instability.

关键词： sodium metal battery sodium metal anode solid electrolyte interphase (SEI) fiber texture film growth

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On-chip unidirectional waveguiding for surface acoustic waves along a defect line in a triangular lattice

arXiv

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arXiv 2021年

作者： Zhou, Yun Zhang, Naiqing Bisharat, Diaaaldin J. Davis, Robert J. Zhang, Zichen Friend, James Bandaru, Prabhakar R. Sievenpiper, Daniel F. Department of Mechanical Engineering University of California San Diego La Jolla CA92093-0411 United States Department of Electrical Engineering University of California San Diego La Jolla CA92093-0411 United States Program in Materials Science University of California San Diego La Jolla CA92093-0411 United States

The latest advances in topological physics have yielded a rich toolset to design highly robust wave transfer systems, for overcoming issues like beam steering and lateral diffraction in surface acoustic waves (SAWs). However, presently used designs for topologically protected SAWs have been largely limited to spin or valley-polarized phases, which rely on non-zero Berry curvature effects. Here we propose and experimentally demonstrate a highly robust SAW waveguide on lithium niobate (LiNbO3), based on a line defect within a true triangular phononic lattice, which instead employs an intrinsic chirality of phase vortices and maintains a zero Berry curvature. The guided SAW mode spans a wide bandwidth (Equation presented) and shows confinement in the lateral direction with 3 dB attenuation within half of the unit-cell length. SAW routing around sharp bends has been demonstrated in such waveguide, with less than ~4% reflection per bend. The waveguide has also been found robust for defect lines with different configurations. The fully on-chip system permits unidirectional SAW modes that are tightly bound to the waveguide, which provides a compact footprint ideal for miniaturization of practical applications and offers insight into the possibility of manipulating highly focused SAW propagation. Copyright © 2021, The Authors. All rights reserved.

关键词： Acoustic waves

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Characteristic of beef tallow/rice husk charcoal/plaster composite phase change materials

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AIP Conference Proceedings 2023年第1期2568卷

作者： I. Made Astika I. Nyoman Suprapta Winaya I. Ketut Gede Wirawan Akhmad Herman Yuwono I. Dewa Gede Ary Subagia 1Doctoral Study Program of Engineering Science Faculty of Engineering Udayana University Kampus Sudirman Denpasar Bali 80234 Indonesia. 2Study Program of Mechanical Engineering Faculty of Engineering Udayana University Kampus Bukit Jimbaran Badung Bali 80361 Indonesia 3Department of Metallurgy and Materials Engineering Faculty of Engineering Universitas Indonesia Kampus Baru UI Depok Depok 16424 Indonesia

Composite materials are a combination of several materials to form a new material with specific properties. Chemical compatibility of the constituent materials is a factor that affects the characteristics of composite materials. Currently, composite phase change materials (PCM) are commonly applied as thermal energy storage systems. This study investigated the characteristics of beef tallow/rice husk charcoal/plaster PCM composite. The weight fraction of rice husk charcoal used was 10%. To prevent leakage of beef tallow from the composite structure, the researcher used polyvinyl acetate (PVAc) to cover the tallow/RHC composite before mixing it with plaster material. As much as 30% of the tallow/RHC/PVAc composite weight fraction is integrated into the plaster. PCM composite was made by using a direct incorporation method. The FTIR test results on the PCM composites produced showed good chemical compatibility among different components. The scanning electron microscope results showed the formation of microcapsules where the rice husk charcoal and polyvinyl acetate functions as a wrapper/capsule for beef tallow. This composite is suitable for application in building walls as thermal energy storage.

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