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Plasmonic bacteria on a nanoporous mirror via hydrodynamic trapping for rapid identification of waterborne pathogens

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Light(Science & Applications) 2018年第1期7卷 359-367页

作者： Keumrai Whang Jong-Hwan Lee Yonghee Shin Wooju Lee Young Wan Kim Dongchoul Kim Luke P.Lee Taewook Kang Department of Chemical and Biomolecular Engineering Sogang UniversitySeoul 04107Korea Berkeley Sensor and Actuator Center Departments of BioengineeringElectrical Engineering and Computer ScienceBiophysics Graduate ProgramUniversity of CaliforniaBerkeleyBerkeleyCA 94720USA Department of Mechanical Engineering Sogang UniversitySeoul 04107Korea

A rapid,precise method for identifying waterborne pathogens is critically needed for effective disinfection and better ***,conventional methods,such as culture-based counting,generally suffer from slow detection times and low ***,we developed a rapid detection method for tracing waterborne pathogens by an innovative optofluidic platform,a plasmonic bacteria on a nanoporous mirror,that allows effective hydrodynamic cell trapping,enrichment of pathogens,and optical signal *** designed and simulated the integrated optofluidic platform to maximize the enrichment of the bacteria and to align bacteria on the nanopores and plasmonic mirror via hydrodynamic cell *** nanoparticles are self-assembled to form antenna arrays on the surface of bacteria,such as Escherichia coli and Pseudomonas aeruginosa,by replacing citrate with hydroxylamine hydrochloride in order to amplify the signal of the plasmonic optical *** to the synergistic contributions of focused light via the nanopore geometry,self-assembled nanoplasmonic optical antennas on the surface of bacteria,and plasmonic mirror,we obtain a sensitivity of detecting *** as low as 102 cells/ml via surface-enhanced Raman *** believe that our label-free strategy via an integrated optofluidic platform will pave the way for the rapid,precise identification of various pathogens.

关键词： mirror trapping precise

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Control of brain network dynamics across diverse scales of space and time

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Physical Review E 2020年第6期101卷 062301-062301页

作者： Evelyn Tang Harang Ju Graham L. Baum David R. Roalf Theodore D. Satterthwaite Fabio Pasqualetti Danielle S. Bassett Department of Bioengineering School of Engineering & Applied Science University of Pennsylvania Pennsylvania 19104 USA Max Planck Institute for Dynamics and Self-Organization Göttingen 37079 Germany Neuroscience Graduate Program Perelman School of Medicine University of Pennsylvania Pennsylvania 19104 USA Department of Psychiatry Perelman School of Medicine University of Pennsylvania Pennsylvania 19104 USA Department of Mechanical Engineering University of California Riverside Riverside California 92521 USA Department of Physics & Astronomy College of Arts & Sciences University of Pennsylvania Pennsylvania 19104 USA Department of Electrical & Systems Engineering School of Engineering & Applied Science University of Pennsylvania Pennsylvania 19104 USA Department of Neurology Perelman School of Medicine University of Pennsylvania Pennsylvania 19104 USA Santa Fe Institute Santa Fe New Mexico 87501 USA

The human brain is composed of distinct regions that are each associated with particular functions and distinct propensities for the control of neural dynamics. However, the relation between these functions and control profiles is poorly understood, as is the variation in this relation across diverse scales of space and time. Here we probe the relation between control and dynamics in brain networks constructed from diffusion tensor imaging data in a large community sample of young adults. Specifically, we probe the control properties of each brain region and investigate their relationship with dynamics across various spatial scales using the Laplacian eigenspectrum. In addition, through analysis of regional modal controllability and partitioning of modes, we determine whether the associated dynamics are fast or slow, as well as whether they are alternating or monotone. We find that brain regions that facilitate the control of energetically easy transitions are associated with activity on short length scales and slow timescales. Conversely, brain regions that facilitate control of difficult transitions are associated with activity on long length scales and fast timescales. Built on linear dynamical models, our results offer parsimonious explanations for the activity propagation and network control profiles supported by regions of differing neuroanatomical structure.

关键词： Network diffusion Synchronization Brain network Magnetic resonance imaging

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Bioelectrical Interfaces Beyond Cellular Excitability: Cancer, Aging, and Gene Expression Reprogramming

arXiv

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arXiv 2025年

作者： Cadinu, Paolo Burgess, Matthew Jones, Catarina Franco Luciani, Titouan Iarossi, Marzia Schröter, Manuel Nakatsuka, Nako Djamgoz, Mustafa B.A. Gonçalves, Gil Sanjuán-Alberte, Paola Mendes, Paula M. Rawson, Frankie J. Nair, Malavika Levin, Michael Moreddu, Rosalia Program in Cellular and Molecular Medicine Boston Children’s Hospital BostonMA02115 United States Department of Microbiology Blavatnik Institute Harvard Medical School BostonMA02115 United States Institute of Biomedical Engineering Department of Engineering Science University of Oxford Oxford United Kingdom Department of Bioengineering Institute for Bioengineering and Biosciences Instituto Superior Técnico University of Lisbon Lisbon Portugal Associate Laboratory i4HB Institute for Health and Bioeconomy Instituto Superior Técnico Universidade de Lisboa Lisbon Portugal Department of Neuroscience Institut Pasteur Paris75015 France Department of Biomedical Engineering Technion Israel Institute of Technology Haifa Israel ETH Zurich Department of Biosystems Science and Engineering Basel Switzerland Laboratory of Chemical Nanotechnology Neuro-X Institute EPFL Genève1202 Switzerland Department of Life Sciences Imperial College London United Kingdom Department of Mechanical Engineering University of Aveiro Aveiro3810-193 Portugal Guimarães4800-058 Portugal School of Chemical Engineering University of Birmingham Birmingham United Kingdom Bioelectronics Laboratory Division of Regenerative Medicine and Cellular Therapies School of Pharmacy Biodiscovery Institute University of Nottingham Nottingham United Kingdom Allen Discovery Centre Tufts University Tufts University MedfordMA United States Wyss Institute for Bioinspired Engineering Harvard University BostonMA United States School of Electronics and Computer Science University of Southampton Southampton United Kingdom Institute for Life Sciences University of Southampton Southampton United Kingdom

Bioelectrical interfaces represent a significant evolution in the intersection of nanotechnology and biophysics, oRering new strategies for probing and influencing cellular processes. These systems capitalize on the subtle but powerful electric fields within living matter, potentially enabling applications beyond cellular excitability, ranging from targeted cancer therapies to interventions in genetic mechanisms and aging. This perspective article envisions the translation, development and application of next-generation solid-state bioelectrical interfaces and their transformative impact across several critical areas of medical research. © 2025, CC BY.

关键词： Medical nanotechnology

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Correction: Assessment of mechanically Induced Changes in Helical Fiber Microstructure Using Diffusion Tensor Imaging

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Annals of biomedical engineering 2024年第4期52卷 845页

作者： Roberto Alonso Pineda Guzman Noel Naughton Shreyan Majumdar Bruce Damon Mariana E Kersh Department of Mechanical Science & Engineering University of Illinois Urbana-Champaign Urbana IL USA. Beckman Institute for Advanced Science & Technology University of Illinois Urbana-Champaign Urbana IL USA. Carle Clinical Imaging Research Program Stephens Family Clinical Research Institute Carle Health Urbana IL USA. Department of Bioengineering University of Illinois Urbana-Champaign Urbana IL USA. Department of Biomedical Engineering Vanderbilt University Nashville TN USA. Vanderbilt University Institute of Imaging Science Vanderbilt University Medical Center Nashville TN USA. Department of Radiology and Radiological Science Vanderbilt University Nashville TN USA. Department of Mechanical Science & Engineering University of Illinois Urbana-Champaign Urbana IL USA. mkersh@illinois.edu. Beckman Institute for Advanced Science & Technology University of Illinois Urbana-Champaign Urbana IL USA. mkersh@illinois.edu. Carle Illinois College of Medicine University of Illinois Urbana-Champaign Urbana IL USA. mkersh@illinois.edu.

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Morphology of living cells cultured on nanowire arrays with varying nanowire densities and diameters

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Science China(Life Sciences) 2018年第4期61卷 427-435页

作者： Zhen Li Henrik Persson Karl Adolfsson Stina Oredsson Christelle N. Prinz Division of Solid State Physics Lund University Lund 22100 Sweden Nano Lund Lund University Lund 22100 Sweden Department of Biology Lund 22362 Sweden Present address: Translational & Bioengineering Program Mechanical and Industrial Engineering University of Toronto Toronto MSG 1M1 ON Canada

Vertical nanowire arrays are increasingly investigated for their applications in steering cell behavior. The geometry of the array is an important parameter, which influences the morphology and adhesion of cells. Here, we investigate the effects of array geometry on the morphology of MCF7 cancer cells and MCF10A normal-like epithelial cells. Different gallium phosphide nanowire array-geometries were produced by varying the nanowire density and diameter. Our results show that the cell size is smaller on nanowires compared to flat gallium phosphide. The cell area decreases with increasing the nanowire density on the substrate. We observed an effect of the nanowire diameter on MCF10A cells, with a decreased cell area on 40 nm diameter nanowires, compared to 60 and 80 nm diameter nanowires in high-density arrays. The focal adhesion morphology depends on the extent to which cells are contacting the substrate. For low nanowire densities and diameters, ceils are lying on the substrate and we observed large focal adhesions at the cell edges. In contrast, for high nanowire densities and diameters, cells are lying on top of the nanowires and we observed point-like focal adhesions distributed over the whole cell. Our results constitute a step towards the ability to fine-tune cell behavior on nanowire arrays.

关键词： nanowires cell morphology diameter density aspect ratio

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Characterizing cellular mechanical phenotypes with mechanonode-pore sensing

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Microsystems & Nanoengineering 2018年第1期4卷 438-449页

作者： Junghyun Kim Sewoon Han Andy Lei Masaru Miyano Jessica Bloom Vasudha Srivastava Martha R.Stampfer Zev J.Gartner Mark A.LaBarge Lydia L.Sohn Department of Mechanical Engineering University of California at BerkeleyBerkeleyCA 94720-1740USA Department of Bioengineering University of California at BerkeleyBerkeleyCA 94720-1762USA Department of Population Sciences Beckman Research InstituteCity of HopeDuarteCA 91010USA Department of Pharmaceutical Chemistry University of CaliforniaSan FranciscoSan FranciscoCA 94143USA Biological Systems and Engineering Division Lawrence Berkeley National LaboratoryCA 94720USA Graduate Program in Bioengineering University of CaliforniaBerkeleyand University of CaliforniaSan FranciscoBerkeleyCA 94720USA

The mechanical properties of cells change with their differentiation,chronological age,and malignant ***,these properties may be useful label-free biomarkers of various functional or clinically relevant cell ***,we demonstrate mechano-node-pore sensing(mechano-NPS),a multi-parametric single-cell-analysis method that utilizes a four-terminal measurement of the current across a microfluidic channel to quantify simultaneously cell diameter,resistance to compressive deformation,transverse deformation under constant strain,and recovery time after *** define a new parameter,the whole-cell deformability index(wCDI),which provides a quantitative mechanical metric of the resistance to compressive deformation that can be used to discriminate among different cell *** wCDI and the transverse deformation under constant strain show malignant MCF-7 and A549 cell lines are mechanically distinct from non-malignant,MCF-10A and BEAS-2B cell lines,and distinguishes between cells treated or untreated with cytoskeleton-perturbing small *** categorize cell recovery time,ΔT_(r),as instantaneous(ΔTr~0 ms),transient(ΔT_(r)≤40 ms),or prolonged(ΔT_(r)>40 ms),and show that the composition of recovery types,which is a consequence of changes in cytoskeletal organization,correlates with cellular *** the wCDI and cell-recovery time,mechano-NPS discriminates between sub-lineages of normal primary human mammary epithelial cells with accuracy comparable to flow cytometry,but without antibody ***-NPS identifies mechanical phenotypes that distinguishes lineage,chronological age,and stage of malignant progression in human epithelial cells.

关键词： microfluidics mechanical phenotyping node-pore sensing biosensors label-free

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Phase imaging with computational specificity (PICS) for measuring dry mass changes in sub-cellular compartments

arXiv

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arXiv 2020年

作者： Kandel, Mikhail E. He, Yuchen R. Lee, Young Jae Chen, Taylor Hsuan-Yu Sullivan, Kathryn Michele Aydin, Onur Saif, Taher M.A. Kong, Hyunjoon Sobh, Nahil Popescu, Gabriel Beckman Institute University of Illinois at Urbana-Champaign UrbanaIL United States Department of Electrical and Computer Engineering University of Illinois at Urbana-Champaign UrbanaIL United States Neuroscience Program University of Illinois at Urbana-Champaign UrbanaIL United States Department of Bioengineering University of Illinois at Urbana-Champaign UrbanaIL United States Department of Mechanical Science and Engineering University of Illinois at Urbana-Champaign UrbanaIL United States Chemical and Biomolecular Engineering University of Illinois at Urbana-Champaign UrbanaIL United States Carl Woese Institute for Genomic Biology University of Illinois at Urbana-Champaign UrbanaIL United States

Due to its specificity, fluorescence microscopy (FM) has become a quintessential imaging tool in cell biology. However, photobleaching, phototoxicity, and related artifacts continue to limit FM’s utility. Recently, it has been shown that artificial intelligence (AI) can transform one form of contrast into another. We present PICS, a combination of quantitative phase imaging and AI, which provides information about unlabeled live cells with high specificity. Our imaging system allows for automatic training, while inference is built into the acquisition software and runs in real-time. Applying the computed fluorescence maps back to the QPI data, we measured the growth of both nuclei and cytoplasm independently, over many days, without loss of viability. Using a QPI method that suppresses multiple scattering, we measured the dry mass content of individual cell nuclei within spheroids. In its current implementation, PICS offers a versatile quantitative technique for continuous simultaneous monitoring of individual cellular components in biological applications where long-term label-free imaging is desirable. Copyright © 2020, The Authors. All rights reserved.

关键词： Fluorescence microscopy

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Design rules for a tunable merged-tip microneedle

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Microsystems & Nanoengineering 2018年第1期4卷 85-94页

作者： Jungeun Lim Dongha Tahk James Yu Dal-Hee Min Noo Li Jeon School of Mechanical and Aerospace Engineering Seoul National UniversitySeoul 08826South Korea Division of WCU Multiscale Mechanical Design Seoul National UniversitySeoul 08826South Korea Institute of Advanced Machinery and Design Seoul National UniversitySeoul 08826South Korea Interdisciplinary Program for Bioengineering Seoul National UniversitySeoul 08826South Korea Department of Chemistry Seoul National UniversitySeoulSouth Korea

This publication proposes the use of an elasto-capillarity-driven self-assembly for fabricating a microscale merged-tip structure out of a variety of biocompatible UV-curable polymers for use as a microneedle *** addition,the novel merged-tip microstructure constitutes a new class of microneedles,which incorporates the convergence of biocompatible polymer micropillars,leading to the formation of a sharp tip and an open cavity capable of both liquid trapping and volume *** combined with biocompatible photopolymer micropillar arrays fabricated with photolithography,elasto-capillarity-driven self-assembly provides a means for producing a complex microneedle-like structure without the use of micromolding or *** publication also explores and defines the design rules by which several fabrication aspects,such as micropillar dimensions,shapes,pattern array configurations,and materials,can be manipulated to produce a customizable microneedle array with controllable cavity volumes,fracture points,and merge *** addition,the incorporation of a modular through-hole micropore membrane base was also investigated as a method for constitutive payload delivery and fluid-sampling *** flexibility and fabrication simplicity of the merged-tip microneedle platform holds promise in transdermal drug delivery applications.

关键词： microstructure needle cavity

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Towards a terramechanics for bio-inspired locomotion in granular environments

arXiv

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arXiv 2019年

作者： Li, Chen Ding, Yang Gravish, Nick Maladen, Ryan D. Masse, Andrew Umbanhowar, Paul B. Komsuoglu, Haldun Koditschek, Daniel E. Goldman, Daniel I. School of Physics Georgia Institute of Technology AtlantaGA30332 United States Interdisciplinary Bioengineering Program Georgia Institute of Technology AtlantaGA30332 United States Department of Mechanical Engineering Northwestern University EvanstonIL60208 United States Department of Electrical and Systems Engineering University of Pennsylvania PhiladelphiaPA19104 United States

Granular media (GM) present locomotor challenges for terrestrial and extraterrestrial devices because they can flow and solidify in response to localized intrusion of wheels, limbs, and bodies. While the development of airplanes and submarines is aided by understanding of hydrodynamics, fundamental theory does not yet exist to describe the complex interactions of locomotors with GM. In this paper, we use experimental, computational, and theoretical approaches to develop a terramechanics for bio-inspired locomotion in granular environments. We use a fluidized bed to prepare GM with a desired global packing fraction, and use empirical force measurements and the Discrete Element Method (DEM) to elucidate interaction mechanics during locomotion-relevant intrusions in GM such as vertical penetration and horizontal drag. We develop a resistive force theory (RFT) to account for more complex intrusions. We use these force models to understand the locomotor performance of two bio-inspired robots moving on and within GM. Copyright © 2019, The Authors. All rights reserved.

关键词： Finite difference method

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An Investigation into Rhythmic and Discrete Gait Using the MIT Skywalker

An Investigation into Rhythmic and Discrete Gait Using the M...

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IEEE International Conference on Biomedical Robotics and Biomechatronics

作者： Brennan Leo Jackson Rui Moura Coelho Hiroaki Hirai Hermano Igo Krebs Heath Science and Technology Program Massachusetts Institute of Technology Department of Mechanical Engineering Massachusetts Institute of Technology Department of Mechanical Sciences and Bioengineering Osaka University

ISBN: (纸本)9781538681848

Stroke is the most common form of central nervous system injury with over 800,000 new strokes occurring each year. It has been shown that experience dependent therapies are capable of promoting brain plasticity and can result in important improvements in motor control and recovery. Our working model postulates that movement is a combination of three different primitives, namely: discrete, rhythmic, and mechanical impedance. We employ this model for both upper and lower extremity training and tailor therapy to a particular patient need by focusing therapy to reduce deficits in the most impaired primitive. By developing therapies that focus on these primitives, we hypothesize that greater motor recovery can be obtained. This study provides a first look at data and attempts to differentiate between rhythmic and discrete walking in healthy subjects. By varying the walking speed, we observed for the two slower speeds a significant (p<0.001) increase in the amount of double stance duration in comparison to the faster speeds, relating to an increased discreteness of movement. Such metric, however, is unaffected when introducing a sidestep, a discrete event amidst a rhythmic movement. This result suggests further investigation into gait biomarkers that may be used to separate these primitives.

关键词： central nervous system injury Primitive working models Synaptic plasticity mechanical impedance Stroke MASSACHUSETTS INSTITUTE OF TECHNOLOGY Locomotion control of movement Gait discrete element Movements Healthy Volunteers

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