We report the development of an implantable multifunctional (glucose and cholesterol) needle type biosensor with integrated RF wireless circuitry for continuous in vivo monitoring of metabolites during short term stay...
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A microdroplet can act as a high quality factor optical cavity that supports Morphology Dependent Resonances (MDRs). Enhanced radiative energy transfer through these optical resonances can also be utilized as a transd...
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A microdroplet can act as a high quality factor optical cavity that supports Morphology Dependent Resonances (MDRs). Enhanced radiative energy transfer through these optical resonances can also be utilized as a transduction mechanism for chemical and biological sensing. Enhancement in radiative energy transfer is observed when a donor/acceptor pair is present in the resonant medium of a microcavity. Here, we demonstrate avidin-biotin binding and its detection through a FRET pair as a potential application for ultra-sensitive detection for fluoroimmunoassays. The binding interaction between the biotinylated donor molecules and streptavidin-acceptor conjugate was used to observe the energy transfer between the dye pairs. The radial modes of MDRs extend to approximately 0.6 r0 inside the droplet. As a result, the fluorescent emission around the center is not coupled to the optical resonances losing sensitivity. To address this problem, we prepared water-in-oil emulsions of avidin and biotin containing solutions. The water phase contains the streptavidin-Alexa Fluor 610 and the oil phase contains biotinylated fluorescent bead. Streptavidin-biotin binding reaction occurs at the water-oil interface. The water phase accumulates at the droplet air interface due to higher specific density enhancing the resonance coupling. Water and oil phase are index-matched to avoid scattering problems. As a result, a large portion of the avidin-biotin complex was localized at the pendant droplet and air interface. Strong coupling of acceptor emission into optical resonances shows that the energy transfer is efficiently mediated through these resonances.
Based on electrical and magnetic properties, such as saturation magnetization, initial permeability, and coercivity, in this work are presented some considerations about the possibilities of applications of nanocrysta...
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The regulations of Formula SAE® competitions restrain design, tuning, and improvement on engine in order to keep safety on driving. The competitors have basically chosen a 600 cc, 4-stroke engine for motorcycle a...
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The goal of the current study was to develop a three dimensional scaffold for creating tissue engineered bioactive bandages. In the current study, we chose polycaprolactone (PCL) as the biomaterial and optimized the e...
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ISBN:
(纸本)0974249211
The goal of the current study was to develop a three dimensional scaffold for creating tissue engineered bioactive bandages. In the current study, we chose polycaprolactone (PCL) as the biomaterial and optimized the electro spinning process parameters: concentration, voltage and the distance between capillary and collection screen. Using scanning electron microscopy (SEM), we measured the fiber diameter and also studied the growth of NIH 3T3 fibroblasts into electrospun PCL scaffolds after various days post seeding. Our studies have been able to obtain uniform fibers of a minimal diameter of 650nm which also provides the best environment for cell growth.
The introduction of engineering Problem Solving for Future Teachers course to assess changes in students' attitudes and knowledge in an engineering for educators class was discussed. Students attitudes towards eng...
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The introduction of engineering Problem Solving for Future Teachers course to assess changes in students' attitudes and knowledge in an engineering for educators class was discussed. Students attitudes towards engineering science were taken through the use of a 20 question survey. Students were presented with statements and had to answer on a 6 point scale ranging from strongly disagree to strongly agree. The results show that students' attitudes to engineering science have significantly improved, and they are more likely to include engineering concepts in their future classes.
A lateral aperture with a round profile ideal for patch clamping is created by isotropic dry etching through a silicon micropartition between two microchannels. A special package design is introduced to establish an a...
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