检索结果-内蒙古大学图书馆

Spatiotemporal Control over Polynucleotide Brush Growth on DNA Origami Nanostructures

Angewandte Chemie 2023年第48期135卷

作者： Dr. Yunqi Yang Dr. Qinyi Lu Yu Chen Marcello DeLuca Prof. Dr. Gaurav Arya Prof. Dr. Yonggang Ke Prof. Dr. Stefan Zauscher Department of Mechanical Engineering and Materials Science Department of Biomedical Engineering Duke University Durham NC 27708 USA Department of Chemistry Emory University Atlanta GA 30322 USA University Program in Materials Science and Engineering Duke University Durham NC 27708 USA Department of Mechanical Engineering and Materials Science Duke University Durham NC 27708 USA Department of Biomedical Engineering Georgia Institute of Technology and Emory University Atlanta GA 30322 USA

DNA nanotechnology provides an approach to create precise, tunable, and biocompatible nanostructures for biomedical applications. However, the stability of these structures is severely compromised in biological milieu due to their fast degradation by nucleases. Recently, we showed how enzymatic polymerization could be harnessed to grow polynucleotide brushes of tunable length and location on the surface of DNA origami nanostructures, which greatly enhances their nuclease stability. Here, we report on strategies that allow for both spatial and temporal control over polymerization through activatable initiation, cleavage, and regeneration of polynucleotide brushes using restriction enzymes. The ability to site-specifically decorate DNA origami nanostructures with polynucleotide brushes in a spatiotemporally controlled way provides access to “smart” functionalized DNA architectures with potential applications in drug delivery and supramolecular assembly.

关键词： DNA Nanostructures Restriction Enzymes Sequential Brush Growth Surface-Initiated Polymerization Terminal Deoxynucleotidyl Transferase

来源：评论

学校读者我要写书评

暂无评论

Electrophoretic deposition (Epd) of natural hydroxyapatite coatings on titanium ti-29nb-13ta-4.6zr substrates for implant material 16th

Electrophoretic deposition (Epd) of natural hydroxyapatite c...

引用

16th International Conference on Quality in Research, QiR 2019

作者： Fajri, Hidayatul Ramadhan, Fuad Nuzul Ficky, N. Juliadmi, Dian Tjong, Djong Hon Manjas, Menkher Affi, Jon Yetri, Yuli Gunawarman Biomedical Science Faculty of Medicine Universitas Andalas Padang Indonesia Mechanical Engineering Dept Faculty of Engineering Universitas Andalas Padang Indonesia Department of Biology Faculty of Science Universitas Andalas Padang Indonesia Department of Surgery Faculty of Medicine Universitas Andalas Padang Indonesia Mechanical Engineering Dept Padang State Polytechnic Padang Indonesia

ISBN: (纸本)9783035715996

This study aims to investigate the effect of the electrophoretic deposition process (EPD) of natural HA (extracted from bovine bones) with various particle size on Ti-29Nb-13Ta-4.6Zr (TNTZ) coating surfaces. HA particles were refined from bovine bone powders using planetary ball mill and then sieving to separate the particle based on its size. The maximum size according to sieving mesh size is #25 µm, #63 µm and #125 µm. The coating process was conducted by using EPD apparatus with voltage and time process 10V and 5 minutes, respectively, for each sample. The coating layer morphology was observed with Stereo Microscopy, Scanning Electron Microscopy (SEM) and the thickness was measured with Thickness Gauge. The result shows that the size of the particle determines the coating layer characteristics. The best of HA coating quality according to the implant coating standard is obtained for the 25 µm particle size with the surface coverage is 99%. The thickness is 121 µm and the ratio of chemical composition Calcium and Phosphor Ca/P) is 1,49%. These may be concluded that, on the point of view physical characteristics, natural HA from bovine bone has potential enough as a coating layer to improve the bioactivity implant for biomedical application. However, the mechanical characteristic of the layer is still needed to determine the strength of coating layer for avoiding delamination during application. © 2020 Trans Tech Publications Ltd, Switzerland.

关键词： Particle size

来源：评论

学校读者我要写书评

暂无评论

Design of flow channel for cell sorter by dielectrophoresis with photolithography technique 24

Design of flow channel for cell sorter by dielectrophoresis ...

引用

24th World Multi-Conference on Systemics, Cybernetics and Informatics, WMSCI 2020

作者： Hashimoto, Shigehiro Matsuzawa, Ryota Endo, Yuji Noji, Takeru Yoshinaka, Kiyoshi Biomedical Engineering Department of Mechanical Engineering Kogakuin University Tokyo163-8677 Japan Health and Medical Research Institute National Institute of Advanced Industrial Science and Technology Tsukuba305-8564 Japan

ISBN: (纸本)9781950492381

The flow channel for cell sorter in vitro has been designed by dielectrophoresis with photolithography technique. A pair of asymmetric surface electrodes of titanium (thickness of 200 nm) were incorporated just before the branching flow channel: a triangular electrode with the tip angle of 0.35 rad, and a rectangular electrode of the flat edge as reference. The cyclic alternating electric current of the square wave (between 0.25 μs and 0.3 μs of periods) was introduced between the surface electrodes. The suspension of C2C12 (mouse myoblast cell line) was injected into the flow channel, and the flow rate was controlled by the pressure head between the inlet and the outlet. Experimental results of the pilot test show that the absolute value of the amplitude of the acceleration by the electric field, which is perpendicular to the flow direction, increases with the radius of the cell. © WMSCI 2020 - 24th World Multi-Conference on Systemics, Cybernetics and Informatics, Proceedings. All rights reserved.

关键词： Electrophoresis

来源：评论

学校读者我要写书评

暂无评论

Microbiota-dependent early-life programming of gastrointestinal motility

引用

iScience 2024年第10期27卷 110895页

作者： Frith, Mary E. Kashyap, Purna C. Linden, David R. Theriault, Betty Chang, Eugene B. Interdisciplinary Scientist Training Program University of Chicago Chicago 60637 IL United States Department of Medicine University of Chicago Chicago 60637 IL United States Division of Gastroenterology and Hepatology Department of Medicine Mayo Clinic Rochester 55905 MN United States Enteric Neuroscience Program Department of Physiology and Biomedical Engineering Mayo Clinic Rochester 55905 MN United States Department of Surgery University of Chicago Chicago 60637 IL United States

Gastrointestinal microbes modulate peristalsis and stimulate the enteric nervous system (ENS), whose development, as in the central nervous system (CNS), continues into the murine postweaning period. Given that adult CNS function depends on stimuli received during critical periods of postnatal development, we hypothesized that adult ENS function, namely motility, depends on microbial stimuli during similar critical periods. We gave fecal microbiota transplantation (FMT) to germ-free mice at weaning or as adults and found that only the mice given FMT at weaning recovered normal transit, while those given FMT as adults showed limited improvements. RNA sequencing (RNA-seq) of colonic muscularis propria revealed enrichments in neuron developmental pathways in mice exposed to gut microbes earlier in life, while mice exposed later—or not at all—showed exaggerated expression of inflammatory pathways. These findings highlight a microbiota-dependent sensitive period in ENS development, pointing to potential roles of the early-life microbiome in later-life dysmotility. © 2024 The Author(s)

关键词： Developmental neuroscience Microbiome Neuroscience

来源：评论

学校读者我要写书评

暂无评论

Promises and technological prospects of two-dimensional Rashba materials

arXiv

引用

arXiv 2024年

作者： Bordoloi, Arjyama Garcia-Castro, A.C. Romestan, Zachary Romero, Aldo H. Singh, Sobhit Department of Mechanical Engineering University of Rochester RochesterNY14627 United States School of Physics Universidad Industrial de Santander Carrera 27 Calle 9 Bucaramanga680002 Colombia Department of Physics and Astronomy West Virginia University MorgantownWV26505-6315 United States Materials Science Program University of Rochester RochesterNY14627 United States

The Rashba spin-orbit coupling effect, primarily arising from structural-inversion asymmetry in periodic crystals, has garnered considerable attention due to its tunability and potential applications in spintronics. Its capability to manipulate electron spin without an external magnetic field opens new avenues for spintronic device design, particularly in semiconductor technology. Within this framework, 2D Rashba materials hold special interest due to their inherent characteristics, which facilitate miniaturization and engineering capabilities. In this Perspective article, we provide an overview of recent advancements in the research of 2D Rashba materials, aiming to offer a comprehensive understanding of the diverse manifestations and multifaceted implications of the Rashba effect in material science. Rather than merely presenting a list of materials, our approach involves synthesizing various viewpoints, assessing current trends, and addressing challenges within the field. Our objective is to bridge the gap between fundamental research and practical applications by correlating each material with the necessary advancements required to translate theoretical concepts into tangible technologies. Furthermore, we highlight promising avenues for future research and development, drawing from insights gleaned from the current state of the field. © 2024, CC BY.

关键词： Magnetic moments

来源：评论

学校读者我要写书评

暂无评论

Deep Learning for High-Speed Laryngeal Imaging Analysis

Deep Learning for High-Speed Laryngeal Imaging Analysis

引用

International Conference on Computational Intelligence and Knowledge Economy (ICCIKE)

作者： Maryam Naghibolhosseini Ahmed M Yousef Mohsen Zayernouri Stephanie RC Zacharias Dimitar D Deliyski Department of Communicative Sciences and Disorders Michigan State University East Lansing MI USA Department of Mechanical Engineering and Statistics and Probability Michigan State University East Lansing MI USA Scottsdale Department of Otolaryngology-Head and Neck Surgery Mayo Clinic Head and Neck Regenerative Medicine Program Mayo Clinic Phoenix AZ USA

High-speed imaging of the larynx provides a valuable means for studying vocal folds function and vibratory behaviors. Using laryngeal high-speed videoendoscopy (HSV) with a flexible nasolaryngoscope, one can record the detailed vibratory movements of vocal folds during connected speech. This high-speed imaging tool enables us to study the normal function of the vocal folds and how this function can be disrupted due to the presence of voice disorders. In this work, HSV data were utilized during connected speech from subjects with normophonic voices (no voice disorders) and a neurological voice disorder. The data were obtained using a high-speed camera, coupled with a flexible endoscope, at 4,000 frames per second. Deep learning was used for the analysis of the big HSV dataset to extract the vibratory behaviors of the vocal folds. This deep-learning-based tool achieved high levels of accuracy for analysis of challenging HSV data in connected speech. This tool provides a computationally cost-effective and an accurate measurement approach that could help design more advanced voice assessment protocols in future.

关键词： Deep learning Image quality Protocols Endoscopes Image edge detection Cameras Larynx

来源：评论

学校读者我要写书评

暂无评论

Plasma Surface Treatment of Cocr and Nicr Alloys for Dental Applications: A Comparative Study of Tin Thin Film Plasma Deposition Techniques

SSRN

引用

SSRN 2024年

作者： Balica, Naasson Matheus Pereira Almeida, Larissa Solano Rossino, Luciana Sgarbi Pupim, Denise Monção, Renan Matos Costa, Thércio Henrique de Carvalho Santos, Rafaela Luiz Pereira Sousa, Rômulo Ribeiro Magalhães Nunes, Lívio César Cunha Federal University of Piauí Technology Center Graduate Program in Materials Science and Engineering Avenida Universitária s/n Ininga PI Teresina Brazil Federal University of São Carlos Graduate Program in Materials Science SP Sorocaba Brazil University of São Paulo Ribeirão Preto School of Dentistry Avenida do Café SP Ribeirão Preto Brazil Federal University of Rio Grande do Norte Technology Center Department of Mechanical Engineering University Campus Natal RN Lagoa Nova Brazil

Cobalt-chromium and nickel-chromium metal alloys have been widely used as biomaterials in the dental field due to their excellent physical-mechanical properties and biocompatibility, as well as the low cost of the material. However, in unfavorable environments, such as the oral cavity, these dental prostheses composed of these alloys can suffer alterations in their structure where they can undergo rapid changes with the surface corrosion process, which can vary with pH and temperature, typically related to changes in the patient's diet. The aim of this work is to carry out a comparative study between the cathodic cage (CC) and hollow cathode (HC) methods, in which a plasma deposition treatment of titanium nitride (TiN) thin films was carried out on CoCr and NiCr samples, in order to subsequently evaluate their surface characteristics and tribological properties in relation to each method. The samples were characterized using X-ray diffractometry (XRD), scanning electron microscopy (SEM/EDS) and micro abrasive wear tests on each sample from the three groups of alloys mentioned. It was observed that the TiN films provided greater resistance to the wear mechanism of all the samples, where through the characterizations, it can be inferred that the two types of treatments carried out on the samples are suitable for dental applications. © 2024, The Authors. All rights reserved.

关键词： Chromium alloys

来源：评论

学校读者我要写书评

暂无评论

Advanced in Vitro Models of Lymphatic Sprouting: Development and Applications of Tissue-Engineered Lymphatic Spheroids for Translational Research

SSRN

引用

SSRN 2024年

作者： Gonzalez-Vargas, Yarelis Hiehle, Greta Parker, Saiid Thompson, Nishone Liu, Alan Neipiyushchikh, Zhanna Dixon, J. Brandon Wallace H. Coulter Department of Biomedical Engineering Georgia Tech AtlantaGA United States Parker H. Petit Institute for Bioengineering and Bioscience Georgia Tech AtlantaGA United States Benjamin E. Mays High School AtlantaGA United States Optics11 Life BostonMA United States George W. Woodruff School of Mechanical Engineering Georgia Tech AtlantaGA United States

The lymphatic system plays a crucial role in maintaining fluid homeostasis, immune cell trafficking, and fat absorption. Lymphangiogenesis, the formation of new lymphatic vessels, occurs primarily by sprouting from pre-existing vessels in adults. While the importance of VEGF-C as a lymphatic-specific mediator has been established, the role of supporting cells and mechanical cues from the tissue microenvironment in guiding lymphatic sprouting remains unclear. The complexity of lymphatic growth and remodeling is further evident in pathological conditions, where various growth factors and cytokines contribute to the process. VEGF-C/VEGFR-3 interaction is a major pathway activated in response to biochemical or biomechanical stimuli, with co-receptors such as neuropilin 2 and integrins playing a role in modulating the signaling. The coordination of lymphatic endothelial cells (LECs) and lymphatic muscle cells (LMCs) during postnatal lymphangiogenesis is a major gap in our understanding of lymphatic biology. While studies have revealed some actuators for muscle cell recruitment, the mechanisms driving this process remain unclear. Most research in this field has relied on in vivo techniques and transgenic animals, limiting reproducibility and the ability to study the complex interactions between cells and their microenvironment. To address these limitations, there is a need for advanced in vitro models that can recapitulate the key features of lymphatic sprouting and provide a platform for studying the underlying mechanisms and testing potential therapeutic interventions. This study investigated tissue-engineered models of in vitro lymphatic sprouting. We developed spheroids from primary lymphatic cells for future tissue engineering applications. We successfully incorporated rat lymphatic endothelial and muscle cells as well as a basement membrane-like coating into a spheroid representative of a collecting lymphatic vessel. A 1:1 spheroid of lymphatic endothelial cells (LE

关键词： Muscle

来源：评论

学校读者我要写书评

暂无评论

Velocity of flowing myoblast cell at oblique micro grooves 24

Velocity of flowing myoblast cell at oblique micro grooves

引用

24th World Multi-Conference on Systemics, Cybernetics and Informatics, WMSCI 2020

作者： Hashimoto, Shigehiro Matsumoto, Taku Endo, Yuji Kuwabara, Shono Yonezawa, Hiroki Kiyoshi, Yoshinaka Biomedical Engineering Department of Mechanical Engineering Kogakuin University Tokyo163-8677 Japan Health and Medical Research Institute National Institute of Advanced Industrial Science and Technology Tsukuba305-8564 Japan

ISBN: (纸本)9781950492381

The velocity change of a flowing cell near the oblique micro groove on the bottom surface in the micro flow channel has been measured in vitro. The micro groove of the rectangular shape (4.5 μm depth, and 0.2 mm length) has been fabricated on the polydimethylsiloxane (PDMS) disk by the photolithography technique. The angle between the flow direction and the longitudinal axis of the groove is 45 degree. Variation has been made on the width (0.03 mm, 0.04 mm, and 0.05 mm) of the groove. A rectangular flow channel (0.05 mm height × 1 mm width × 25 mm length) has been constructed between two transparent PDMS disks. In the test, malnourished C2C12 (mouse myoblast cell line) was used in comparison with the normal C2C12. A flow velocity (0.02 mm/s © WMSCI 2020 - 24th World Multi-Conference on Systemics, Cybernetics and Informatics, Proceedings. All rights reserved.

关键词： Polydimethylsiloxane

来源：评论

学校读者我要写书评

暂无评论

Corrigendum to "Computational Modeling of Mouse Colorectum Capturing Longitudinal and Through-thickness Biomechanical Heterogeneity" [J. Mech. Behav. Biomed. Mater. 113 (2021) 104127]

引用

Journal of the mechanical behavior of biomedical materials 2024年 154卷 106287页

作者： Amirhossein Shokrani Ashkan Almasi Bin Feng David M Pierce School of Mechanical Aerospace and Manufacturing Engineering University of Connecticut Storrs CT USA. Department of Biomedical Engineering University of Connecticut Storrs CT USA. School of Mechanical Aerospace and Manufacturing Engineering University of Connecticut Storrs CT USA Department of Biomedical Engineering University of Connecticut Storrs CT USA. Electronic address: david.pierce@uconn.edu.

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：