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Bioceramic-mediated chondrocyte hypertrophy promotes calcified cartilage formation for rabbit osteochondral defect repair

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Bioactive Materials 2024年第10期40卷 306-317页

作者： Rachel H.Koh Junhee Kim Jeong-Uk Kim Seunghyun L.Kim Arun Kumar Rajendran Seunghun SLee Heesoo Lee Joo Hyun Kim Ji Hoon Jeong Yongsung Hwang Jong Woo Bae Nathaniel S.Hwang School of Chemical and Biological Engineering Institute of Chemical ProcessesSeoul National UniversitySeoul08826South Korea Interdisciplinary Program in Bioengineering Seoul National UniversitySeoul08826South Korea Department of Biomedical Engineering Dongguk UniversitySeoul10326South Korea Soonchunhyang Institute of Medi-bio Science(SIMS) Soonchunhyang UniversityCheonan31151South Korea Department of Integrated Biomedical Science Soonchunhyang UniversityAsan31538South Korea Department of Orthopaedic Surgery Konkuk University Chungju HospitalKonkuk University School of MedicineChungju27376South Korea BioMAX Institute Seoul National UniversitySeoul08826South Korea

Osteochondral tissue is a highly specialized and complex tissue composed of articular cartilage and subchondral bone that are separated by a calcified cartilage *** or gradient scaffolds,often in conjunction with stem cells and growth factors,have been developed to mimic the respective layers for osteochondral defect *** this study,we designed a hyaline cartilage-hypertrophic cartilage bilayer graft(RGD/RGDW)with ***,we demonstrated that RGD peptide-modified chondroitin sulfate cryogel(RGD group)is chondro-conductive and capable of hyaline cartilage ***,we incorporated whitlockite(WH),a Mg^(2+)-containing calcium phosphate,into RGD cryogel(RGDW group)to induce chondrocyte hypertrophy and form collagen X-rich hypertrophic *** is the first study to use WH to produce hypertrophic ***-laden RGDW cryogel exhibited significantly upregulated expression of hypertrophy markers in vitro and formed ectopic hypertrophic cartilage in vivo,which mineralized into calcified cartilage in bone ***,RGD cryogel and RGDW cryogel were combined into bilayer(RGD/RGDW group)and implanted into rabbit osteochondral defect,where RGD layer supports hyaline cartilage regeneration and bioceramic-containing RGDW layer promotes calcified cartilage *** the RGD group(monolayer)formed hyaline-like neotissue that extends into the subchondral bone,the RGD/RGDW group(bilayer)regenerated hyaline cartilage tissue confined to its respective layer and promoted osseointegration for integrative defect repair.

关键词： Bioceramic Whitlockite Bilayer scaffold Chondrocyte hypertrophy Osteochondral defect repair

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Mechano-responsive hydrogel for direct stem cell manufacturing to therapy

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Bioactive Materials 2023年第6期24卷 387-400页

作者： Yufeng Shou Ling Liu Qimin Liu Zhicheng Le Khang Leng Lee Hua Li Xianlei Li Dion Zhanyun Koh Yuwen Wang Tong Ming Liu Zheng Yang Chwee Teck Lim Christine Cheung Andy Tay Department of Biomedical Engineering National University of Singapore117583Singapore Institute for Health Innovation&Technology National University of Singapore117599Singapore School of Civil Engineering and Architecture Wuhan University of Technology430070WuhanChina NUS Tissue Engineering Program National University of Singapore117510Singapore Lee Kong Chian School of Medicine Nanyang Technological University636921Singapore School of Mechanical and Aerospace Engineering Nanyang Technological University639798Singapore Institute of Molecular and Cell Biology Agency for ScienceTechnology and Research(A*STAR)138648Singapore Department of Orthopaedic Surgery Yong Loo Lin School of MedicineNational University of Singapore119288Singapore Mechanobiology Institute National University of Singapore117411Singapore

Bone marrow-derived mesenchymal stem cell(MSC)is one of the most actively studied cell types due to its regenerative potential and immunomodulatory *** cell expansion methods using 2D tissue culture plates and 2.5D microcarriers in bioreactors can generate large cell numbers,but they compromise stem cell potency and lack mechanical preconditioning to prepare MSC for physiological loading expected in *** overcome these challenges,in this work,we describe a 3D dynamic hydrogel using magneto-stimulation for direct MSC manufacturing to *** our technology,we found that dynamic mechanical stimulation(DMS)enhanced matrix-integrinβ1 interactions which induced MSCs spreading and *** addition,DMS could modulate MSC biofunctions including directing MSC differentiation into specific lineages and boosting paracrine activities(e.g.,growth factor secretion)through YAP nuclear localization and FAK-ERK *** our magnetic hydrogel,complex procedures from MSC manufacturing to final clinical use,can be integrated into one single platform,and we believe this‘all-in-one’technology could offer a paradigm shift to existing standards in MSC therapy.

关键词： Mesenchymal stem cell Dynamic mechanical stimulation Magnetic hydrogel Stem cell manufacturing Cell therapy

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Functional Peptide-Loaded Gelatin Nanoparticles as Eyedrops for Cornea Neovascularization Treatment

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International Journal of Nanomedicine 2023年 18卷 1413-1431页

作者： Chu, Ya-Chun Fang, Hsu-Wei Wu, Yu-Yi Tang, Yu-Jun Hsieh, Erh-Hsuan She, Yizhou Chang, Che-Yi Lin, I-Chan Chen, Yin-Ju Liu, Guei-Sheung Tseng, Ching-Li Graduate Institute of Biomedical Materials and Tissue Engineering College of Biomedical Engineering Taipei Medical University Taipei City Taiwan Department of Chemical Engineering and Biotechnology National Taipei University of Technology Taipei City Taiwan Institute of Biomedical Engineering and Nanomedicine National Health Research Institutes Miaoli County Taiwan School of Biomedical Engineering College of Biomedical Engineering Taipei Medical University Taipei City Taiwan Department of Ophthalmology Wan Fang Hospital Taipei Medical University Taipei City Taiwan Department of Ophthalmology School of Medicine College of Medicine Taipei Medical University Taipei City Taiwan Department of Radiation Oncology Taipei Medical University Hospital Taipei City Taiwan International Ph.D. Program in Biomedical Engineering College of Biomedical Engineering Taipei Medical University Taipei City Taiwan Center for Precision Medicine and Translational Cancer Research Taipei Medical University Hospital Taipei City Taiwan Centre for Eye Research Australia Royal Victorian Eye and Ear Hospital East Melbourne VIC Australia Ophthalmology Department of Surgery University of Melbourne East Melbourne VIC Australia Menzies Institute for Medical Research University of Tasmania Hobart TAS Australia Research Center of Biomedical Device College of Biomedical Engineering Taipei Medical University Taipei City Taiwan

Background: Corneal neovascularization (NV) is a process of abnormal vessel growth into the transparent cornea from the limbus and can disturb the light passing through the cornea, resulting in vision loss or even blindness. The use of nanomedicine as an effective therapeutic formulation in ophthalmology has led to higher drug bioavailability and a slow drug release rate. In this research, we designed and explored the feasibility of a new nanomedicine, gp91 ds-tat (gp91) peptide-encapsulated gelatin nanoparticles (GNP-gp91), for inhibiting corneal angiogenesis. Methods: GNP-gp91 were prepared by a two-step desolvation method. The characterization and cytocompatibility of GNP-gp91 were analyzed. The inhibition effect of GNP-gp91 on HUVEC cell migration and tube formation was observed by an inverted microscope. The drug retention test in mouse cornea was observed by in vivo imaging system, fluorescence microscope, and DAPI/TAMRA staining. Finally, the therapeutic efficacy and evaluation of neovascularization-related factors were conducted through the in vivo corneal NV mice model via topical delivery. Results: The prepared GNP-gp91 had a nano-scale diameter (550.6 nm) with positive charge (21.7 mV) slow-release behavior (25%, 240hr). In vitro test revealed that GNP-gp91 enhanced the inhibition of cell migration and tube formation capacity via higher internalization of HUVEC. Topical administration (eyedrops) of the GNP-gp91 significantly prolongs the retention time (46%, 20 min) in the mouse cornea. In chemically burned corneal neovascularization models, corneal vessel area with a significant reduction in GNP-gp91 group (7.89%) was revealed when compared with PBS (33.99%) and gp91 (19.67%) treated groups via every two days dosing. Moreover, GNP-gp91 significantly reduced the concentration of Nox2, VEGF and MMP9 in NV’s cornea. Conclusion: The nanomedicine, GNP-gp91, was successfully synthesized for ophthalmological application. These data suggest that GNP-gp91 contain

关键词： control release corneal neovascularization eye drops gelatin gp91 peptide nanoparticles ocular retention

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Soft Tubular Strain Sensors for Contact Detection 12th

Soft Tubular Strain Sensors for Contact Detection

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Proceedings of the 12th International Conference on Biomimetic and Biohybrid Systems, Living Machines 2023

作者： Dai, Kevin Elangovan, Abirami Whirley, Karen Webster-Wood, Victoria A. Department of Mechanical Engineering Carnegie Mellon University Pittsburgh United States Department of Biomedical Engineering Carnegie Mellon University PittsburghPA United States McGowan Institute for Regenerative Medicine Carnegie Mellon University PittsburghPA United States

ISBN: (纸本)9783031388569

Sensing and actuation are intricately connected in soft robotics, where contact may change actuator mechanics and robot behavior. To improve soft robotic control and performance, proprioception and contact sensors are needed to report robot state without altering actuation mechanics or introducing bulky, rigid components. For bioinspired McKibben-style fluidic actuators, prior work in sensing has focused on sensing the strain of the actuator by embedding sensors in the actuator bladder during fabrication, or by adhering sensors to the actuator surface after fabrication. However, material property mismatches between sensors and actuators can impede actuator performance, and many soft sensors available for use with fluidic actuators rely on costly or labor-intensive fabrication methods. Here, we demonstrate a low-cost and easy-to manufacture-tubular liquid metal strain sensor for use with soft actuators that can be used to detect actuator strain and contact between the actuator and external objects. The sensor is flexible, can be fabricated with commercial-off-the-shelf components, and can be easily integrated with existing soft actuators to supplement sensing, regardless of actuator shape or size. Furthermore, the soft tubular strain sensor exhibits low hysteresis and high sensitivity. The approach presented in this work provides a low-cost, soft sensing solution for broad application in soft robotics. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2023.

关键词： Actuators

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ADDITIVE FRICTION STIR DEPOSITION DYNAMOMETER DESIGN AND EVALUATION 39

ADDITIVE FRICTION STIR DEPOSITION DYNAMOMETER DESIGN AND EVA...

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39th Annual Meeting of the American Society for Precision engineering, ASPE 2024

作者： Woodard, Tyler Edwards, Bradley Patterson, Matthew R. Charles, Elijah Schmitz, Tony Mechanical Aerospace and Biomedical Engineering Department University of Tennessee KnoxvilleTN37996 United States Manufacturing Demonstration Facility Oak Ridge National Laboratory Oak RidgeTN37830 United States

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Bioinspired Rotation Microneedles for Accurate Transdermal Positioning and Ultraminimal-Invasive Biomarker Detection with mechanical Robustness

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Research 2022年第3期2022卷 45-59页

作者： Yilin Cai Shiyi Huang Zhinan Zhang Jiazheng Zhang Xingyue Zhu Xiaoxiang Chen Xianting Ding State Key Laboratory of Mechanical System and Vibration School of Mechanical EngineeringShanghai Jiao Tong UniversityShanghai 200240China State Key Laboratory of Oncogenes and Related Genes Institute for Personalized MedicineSchool of Biomedical EngineeringShanghai Jiao Tong UniversityShanghai 200030China Department of Rheumatology Renji HospitalShanghai Jiao Tong University School of MedicineShanghaiChina

Microneedle permits transdermal biosensing and drug delivery with minor ***,accurate microneedle transdermal positioning with minimal skin deformation remains a significant technical challenge due to inhomogeneous skin topology and discontinuous force applied to the ***,we introduce bioinspired rotation microneedles for in vivo accurate microneedle positioning as inspired by honeybees’*** demonstrate the benefits of rotation microneedles in alleviating skin resistance through finite element analysis,full-thickness porcine validations,and mathematical derivations of microneedleskin interaction stress *** max penetration force was mitigated by up to 45.7%and the force attenuation rate increased to 2.73 times in the holding stage after penetration.A decrease in max skin deflection and a faster deformation recovery introduced by rotation microneedles implied a more precise penetration ***,we applied the rotation microneedles in psoriasis mice,a monogenic disorder animal model,for minimally invasive biological sample extraction and proinflammatory cytokine *** ultrasensitive detection method is realized by using only one microneedle to achieve cytokine mRNA level determination compared to commonly required biopsies or blood ***,rotation microneedles permit a simple,rapid,and ultraminimal-invasive method for subcutaneous trace biological sample acquisition and subsequent point-of-care diagnostics with minimal damage to both microneedles and skins.

关键词： rotation precise faster

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Viscoelastic Characterization of an Agar Tissue Phantom Using Acoustic Microelastography

Viscoelastic Characterization of an Agar Tissue Phantom Usin...

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International Conference on Manipulation, Automation and Robotics at Small Scales (MARSS)

作者： Bhas N. Karmarkar Moustafa Sayed Ahmed John Reynolds Michael Bortner Shima Shahab Bahareh Behkam Department of Mechanical Engineering Virginia Tech Blacksburg VA USA Department of Chemical Engineering Virginia Tech Blacksburg VA USA Department of Mechanical Engineering School of Biomedical Engineering and Sciences Virginia Tech Blacksburg VA USA

ISBN: (数字)9798350376807

ISBN: (纸本)9798350376814

Elastography has long been used as a diagnostic tool in the clinic. This technique assesses tissue elasticity qualitatively or quantitatively in several pathologies, including liver fibrosis and some cancers. Most acoustic elastography approaches are based on the overall evaluation of the region/organ of interest. In contrast, microelastography is a technique that enables quantification of the stiffness at microscale spatial resolution. Acoustic impedance, the main component responsible for ultrasound imaging, varies with bulk modulus and mass density; thus, it can be used to characterize the material properties. However, in situ, spatial mapping of tissue properties at the microscale has rarely been studied. In this work, we combined acoustic actuation, high spatiotemporal resolution imaging, and computer vision to enable quantitative mapping of the mechanical properties of agar tissue phantoms at the microscale. We used a customized electrodynamic resonant vibration actuator at 2065 Hz to generate Rayleigh waves in the tissue phantoms, which were recorded at 9501 frames per second (fps). The resulting videos were analyzed using computer vision tools to quantify the speed of the acoustic shear wave and determine the shear modulus and viscosity of agar with an input of density. To validate our microelastography measurements, we performed oscillatory rheology using a parallel plate rheometer from 0.1-100 Hz, quantified the dynamic viscoelastic properties of agar, and extrapolated the measurements to the acoustic microelastography working frequency. The results from the two methods are in good agreement, showing a difference of 4.7%. In the future, we will adapt this technique for the spatial evaluation of mechanical properties of heterogeneous cancerous tissues at higher resolutions. Such capability can enable effective diagnosis and development of patient-specific treatment regimens.

关键词： Viscosity Computer vision Phantoms biomedical measurement Acoustic measurements Acoustics Frequency measurement

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Volume holographic illuminator for Airy light-sheet microscopy

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Optics Express 2024年第1期32卷 167-178页

作者： Hsu, Hung-Chuan Vyas, Sunil Wu, Jui-Ching Huang, Kuang-Yuh Liao, Hsien-Shun Yeh, J. Andrew Luo, Yuan Department of Mechanical Engineering National Taiwan University Taipei10617 Taiwan Institute of Medical Devices and Imaging System National Taiwan University Taipei10051 Taiwan Department of Clinical Laboratory Science and Medical Biotechnology National Taiwan University Taipei10048 Taiwan Institute of Nano Engineering and Microsystems National Tsing Hua University Hsinchu30013 Taiwan YongLin Institute of Health National Taiwan University Taipei10087 Taiwan Department of Biomedical Engineering National Taiwan University Taipei10051 Taiwan Graduate School of Advanced Technology National Taiwan University Taipei106319 Taiwan

Airy light sheets combined with the deconvolution approach can provide multiple benefits, including large field of view (FOV), thin optical sectioning, and high axial resolution. The efficient design of an Airy light-sheet fluorescence microscope requires a compact illumination system. Here, we show that an Airy light sheet can be conveniently implemented in microscopy using a volume holographic grating (VHG). To verify the FOV and the axial resolution of the proposed VHG-based Airy light-sheet fluorescence microscope, ex-vivo fluorescently labeled Caenorhabditis elegans (C. elegans) embryos were imaged, and the Richardson-Lucy deconvolution method was used to improve the image contrast. Optimized parameters for deconvolution were compared with different methods. The experimental results show that the FOV and the axial resolution were 196 µm and 3 µm, respectively. The proposed method of using a compact VHG to replace the common spatial light modulator provides a direct solution to construct a compact light-sheet fluorescence microscope. © 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.

关键词： Image enhancement

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Bioengineering approaches for patient-specific analysis of placenta structure and function

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Placenta 2025年 166卷 154-163页

作者： Scott, Adrienne K. Fodera, Daniella M. Yang, Patrick Arter, Abigail Hines, Amelia M. Kolluru, Samyuktha S. Zambuto, Samantha G. Myers, Kristin M. Kamilov, Ulugbek S. Odibo, Anthony O. Oyen, Michelle L. Department of Biomedical Engineering Washington University in St. Louis St. Louis MO United States Center for Women's Health Engineering Washington University in St. Louis St. Louis MO United States Center for Regenerative Medicine Washington University in St. Louis St. Louis MO United States Department of Biomedical Engineering Columbia University New York NY United States Department of Obstetrics and Gynecology Washington University School of Medicine in St. Louis St. Louis MO United States Department of Mechanical Engineering Columbia University New York NY United States Department of Computer Science & Engineering Washington University in St. Louis St. Louis MO United States Department of Electrical & Systems Engineering Washington University in St. Louis St. Louis MO United States

The leading cause of perinatal mortality is fetal growth restriction (FGR), defined as in utero fetal growth below the 10th percentile. Insufficient exchange of oxygen and nutrients at the maternal-fetal interface is associated with FGR. This transport occurs through the vasculature of the placenta, particularly in the terminal villi, where the vascular membranes have a large surface area and are the thinnest. Altered structure of the placenta villi is thought to contribute to decreased oxygen exchange efficiency, however, understanding how the three-dimensional microstructure and properties decrease this efficiency remains a challenge. Here, a novel, multiscale workflow is presented to quantify patient-specific biophysical properties, 3D structural features, and blood flow of the villous tissue. Namely, nanoindentation, optical coherence tomography, and ultrasound imaging were employed to measure the time-dependent material properties of placenta tissue, the 3D structure of villous tissue, and blood flow through the villi to characterize the microvasculature of the placenta at increasing length scales. Quantifying the biophysical properties, the 3D architecture, and blood flow in the villous tissue can be used to infer changes in maternal-fetal oxygen transport at the villous membrane. Overall, this multiscale understanding will advance knowledge of how microvascular changes in the placenta ultimately lead to FGR, opening opportunities for diagnosis and intervention. © 2024 The Authors

关键词： Doppler ultrasound Fetal growth restriction Nanoindentation Optical coherence tomography Placenta Villi

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Negative Poisson’s ratio locally resonant seismic metamaterials vibration isolation barrier

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Acta Mechanica Sinica 2024年第8期40卷 239-252页

作者： Haibin Ding Nianyong Huang Muhammad Changjie Xu Lihong Tonog State Key Laboratory of Performance Monitoring and Protecting of Rail Transit Infrastructure Nanchang330013China Institute of Geotechnical Engineering School of Civil Engineering and ArchitectureEast China Jiaotong UniversityNanchang330013China Department of Mechanical Manufacturing and Biomedical EngineeringTrinity College DublinDublinD02PN40Ireland Department of Mechanical Engineering University of GalwayUniversity RoadGalwayH91 HX31Ireland

In recent decades,the application of seismic metamaterials to protect civil infrastructures being free of the damage of earthquakes has been attracting extensive ***,the proposed locally resonant seismic metamaterials provide the probability of isolating the low-frequency seismic wave using a small-size isolation ***,in previous studies,the energy absorption properties of locally resonant seismic metamaterials remain one of the least understood aspects of *** from the fascinating energy absorption characteristic of negative Poisson ratio(NPR)metamaterial,we creatively design a new seismic metamaterial structure by assembling the locally resonant seismic metamaterial and NPR metamaterial,to isolate seismic *** sound cone technique combining the transmission spectrum is employed to identify the surface wave from the hybrid *** generation mechanism of frequency bandgap and the isolation effectiveness of the proposed seismic metamaterial are discussed in *** results indicate that the generation of ultra-low and ultra-wide frequency bandgap with the range of 0.65 Hz–18.9 Hz is attributed to the locally resonant and energy absorption of the proposed seismic metamaterial structure and the excellent isolation effect is achieved by transforming the surface wave into the bulk *** frequency bandgap narrows as the distance increases between each *** addition,the mechanical properties of the NPR bearing,such as the Poisson ratio,mass density,and elastic modulus,have remarkable impact on the frequency bandgap,especially on the upper bound *** practical engineering,the NPR bearing with a low Poisson ratio,small mass density,and high elastic modulus is suggested for the design of the NPR locally resonant seismic metamaterial *** domain analysis for the practical seismic wave verifies that the proposed seismic metamaterial has a promising application in isolating ultra-low and ultra-wi

关键词： Seismic metamaterials Locally resonant Negative Poisson ratio(NPR) Seismic waves Frequency bandgap Vibration isolation barrier

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