Traditional materials have certain restrictions in an industrial application today inhibiting them from the attainment of the best blend of toughness, density and strength. The paradigm shift in the use of agro-waste ...
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Paralysis leads to loss of motor or sensory function, and electric wheelchairs help restore mobility. With advancing technology, electric wheelchairs can now be controlled using myoelectric signals from muscles like t...
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Breast cancer is a global health problem. Mexican statistics show that 28.2% of new cancer cases in women come from the breast, so efforts must be made to control the burden of the disease. Early detection is an essen...
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Patient safety arises from the need to prevent and protect patients from avoidable mistakes. Two fundamental concepts that shape patient safety are error and adverse events, the consequences may range from potentially...
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Liquid crystal display (LCD)-based vat photopolymerization (VPP) is a versatile and accessible 3D printing technology due to its low cost and fast printing speed. It is ideal for applications requiring rapid fabricati...
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Spectroscopic single-molecule localization microscopy allows multi-color super-resolution images with high spectral sensitivity. While its profile has been steadily growing, it is still under-utilized in biology labs,...
Spectroscopic single-molecule localization microscopy allows multi-color super-resolution images with high spectral sensitivity. While its profile has been steadily growing, it is still under-utilized in biology labs, and the complexity of adoption for existing is prohibitive. In this protocol, we provide essential information for researchers to implement sSMLM in a laboratory setting. We describe how to assemble and optically align the illumination and detection paths of a 3D dual-wedge prism-based sSMLM instrument. We provide detailed step-by-step instructions on performing spectral and axial calibration using fluorescent beads and a nanohole array, respectively. We also discuss using sSMLM to image fluorescently labeled cells and report a new MATLAB package RainbowSTORM v2 to reconstruct super-resolution 3D images. Further, we present representative images as typical anticipated results. By demonstrating an implementation of spectroscopic single-molecule localization microscopy that is simple to implement with commercial microscope systems using freely distributed hardware and by introducing new calibration and image processing user interfaces, we hope to remove some of the barriers to entry that biological labs face when entering the spectroscopic super-resolution field. Ultimately, this protocol will allow users to achieve multi-color super-resolution imaging using a single excitation wavelength.
Most infectious diseases are caused by viruses that are spread from one person to another through the air. Isolation room is a room that keeps virus in infected people from spreading to other people by maintaining tem...
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Microrobots have emerged as promising tools for biomedical and in vivo applications, leveraging their untethered actuation capabilities and miniature size. Despite extensive research on diversifying multi-actuation mo...
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Microrobots, untethered miniature devices capable of performing tasks at the microscale, have gained significant attention in the fields of robotics and biomedicine. These devices hold immense potential for various in...
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