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Propolis nanoemulsion extract from celebes stingless bee (Tetragonula biroi) phytochemistry and antibacterial analysis to periodontopathogen bacteria

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Journal of Oral Biology and Craniofacial Research 2025年第3期15卷 576-584页

作者： Amalia, Nadya Rafika Budhy, Theresia Indah Ridwan, Rini Devijanti Rianti, Devi Bramantoro, Taufan Luthfi, Muhammad Ramadhani, Nastiti Faradilla Pramusita, Adya Putranti, Nurul Aisyah Rizki Nugraha, Albertus Putera Situmorang, Putri Cahaya Shariff, Khairul Anuar Nugraha, Alexander Patera Dental Health Science Study Program Faculty of Dental Medicine Universitas Airlangga Surabaya Indonesia Department of Oral and Maxillofacial Pathology Faculty of Dental Medicine Universitas Airlangga Surabaya Indonesia Department of Oral Biology Faculty of Dental Medicine Universitas Airlangga Surabaya Indonesia Department of Dental Material Faculty of Dental Medicine Universitas Airlangga Surabaya Indonesia Department of Dental Public Health Faculty of Dental Medicine Universitas Airlangga Surabaya Indonesia Department of Dentomaxillofacial Radiology Faculty of Dental Medicine Universitas Airlangga Surabaya Indonesia Department of Orthodontic Faculty of Dental Medicine Universitas Airlangga Surabaya Indonesia Faculty of Medicine Universitas Airlangga Surabaya Indonesia Biology Study Program Faculty of Mathematics and Natural Sciences Universitas Sumatera Utara Medan Indonesia School of Materials and Mineral Resources Engineering Engineering Campus Universiti Sains Malaysia Penang Nibong Tebal 14300 Malaysia

Background: Propolis from Sulawesi's stingless bees (Tetragonula biroi) contains antioxidants, more flavonoids than propolis from Apis bees, and the antibacterial ability. Objective: to examine the antibacterial properties of Propolis Nanoemulsion Extract (PNE), which is extracted from the Celebes Stingless Bee (T. biroi), in relation to the periodontopathogen bacteria such as Fusobacterium nucleatum (Fn), Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitans (Aa), and Provotella intermedia (Pi). This investigation also examines PNE's phytochemistry, particle size analysis (PSA), and zeta potential. Methods: The maceration process with 96 % ethanol was used to create PNE from Celebes stingless bee (T. biroi), which was then subjected to zeta potential measurement and PSA. Phytochemical analysis was used to identify phytochemical constituents in the PNE (T. biroi). Diffusion zone, minimum bactericidal concentration (MBC), and minimum inhibitory concentration (MIC) were used to assess antibacterial efficacy against Aa, Pg, Pi, and Fn. Furthermore, the statistical analysis was used to extract the data. Results: Phenols, alkaloids, and flavonoids were identified;however, triterpenoids and saponins were not. Between 151.28 and 182.2 diameter nanometers (***) was the range of the PNE's diameter. At 1.56 % propolis (T. biroi) concentration, the MIC, MBC, and diffusion zone analysis performed better than at 0.76 %, with a significant difference (p:0.01;p < 0.05) to Aa, Pg, Pi, and Fn. Conclusions: The highest antibacterial activity against Aa, Pg, Pi, and Fn as periodontopathogen bacteria is demonstrated by alkaloids, flavonoids, and phenols in PNE from Celebes (T. biroi) at a concentration of 1.56 %. © 2025 The Authors

关键词： Antibacterial Communicable disease Infectious disease Medicine Periodontal disease

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Process Optimization and Scaling of Molybdenum and Tungsten in Additive Manufacturing Techniques

SSRN

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SSRN 2024年

作者： Islam, Zahabul Kunkel, William Hatler, Caleb Thoma, Dan J. Mechanical and Manufacturing Engineering Program School of Engineering Bowling Green State University Bowling GreenOH43403 United States Alloy Design and Development Laboratory Department of Materials Science and Engineering University of Wisconsin MadisonWI53706 United States

Metal additive manufacturing processes pose a daunting challenge in defining optimal process parameters that yield porosity defect-free, dense parts. This is particularly true in refractory metals, where high melting temperatures limit process parameter windows. This present study investigates a dimensionless number analysis that has been derived through the Buckingham-PI theorem and demonstrates a robust correlation between process parameters and the density of the fabricated components. The introduction of this dimensionless number enables the initial estimation of process parameters without extensive modeling or experimentation. Additionally, a universal scaling law is proposed to enhance the translation of predicting process parameters for achieving an optimal density in as-built parts between different processing techniques. A combination of this proposed dimensionless number and scaling law offers process maps for tungsten and molybdenum with previously unknown process parameters. The universality of the proposed dimensionless number also demonstrates transferability, enabling the prediction of process parameters both for laser powder bed fusion and directed energy deposition systems. © 2024, The Authors. All rights reserved.

关键词： 3D printing

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Evolution of 14-connected Zr₆ Secondary Building Units through Postsynthetic Linker Incorporation (Jun, 10.1021/acsami.1c07701, 2021)

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ACS APPLIED materials & INTERFACES 2021年第33期13卷 40155-40155页

作者： Hurlock, Matthew J. Hao, Leiduan Kriegsman, Kyle W. Guo, Xiaofeng O'Keeffe, Michael Zhang, Qiang Department of Chemistry Washington State University Pullman Washington 99164 United States Department of Chemistry Materials Science and Engineering Program Washington State University Pullman Washington 99164 United States School of Molecular Sciences Arizona State University Tempe Arizona 85287 United States

Two new zirconium MOFs, WSU-6 and WSU-7, were synthesized through postsynthetic modifications. In both cases, linker insertion was conducted on a MOF consisting of eight-connected (8-c) Zr6 cluster and four-connected (4-c) ETTC linker, WSU-5, which possesses the uncommon 4, 8-c scu-c topology. The insertion of 1, 4-benzenedicarboxylate into the MOF formed the new 4, 12-c mjh topology, WSU-6. Interestingly, when 2, 6-naphthalenedicarboxylate was inserted, WSU-7 can be formed, which possesses a new 4, 14-c jkz topology. WSU-7 contains very rare 14-c Zr6 secondary building units (SBUs) and is the first MOF to have a Zr6 SBUs with connectivity greater than 12. The three Zr-MOFs were structurally characterized, and the photoluminescence properties of the materials were also studied.

关键词： metal−organic frameworks postsynthetic ligand insertion new topology 14-connected Zr6 SBU luminescence

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An Anti-Plasma Etching Film of Y2O3 Film

An Anti-Plasma Etching Film of Y2O3 Film

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International Conference on Applied System Innovation (ICASI)

作者： Chia-Chih Wei Hsuan-Jung Chen Yi-Mu Lee Shao-Fu Chang Chien-Chon Chen College of Engineering and Science Ph.D. Program in Materials and Chemical Engineering National United University Miaoli Taiwan Department of Chemistry Michigan State University MI USA Department of Electronic National United University Miaoli Taiwan Department of Energy Engineering National United University Miaoli Taiwan

ISBN: (数字)9798350394924

ISBN: (纸本)9798350394931

In this paper, we have presented our experiment results including (a) use a slowly thermal spray process deposition an yttrium oxide (Y 2 O 3 ) film with thickness of $200\ \mu\mathrm{m}$ on the aluminum alloy (AA6061). The image analysis showed that the porosity of Y 2 O 3 film can be reduced to less than 2%. The further aerosol deposition can be used filling the pores and can reduce the porosity of the film to less than 0.5%. This low-porosity Y 2 O 3 film will be able to meet the increasingly stringent requirements for surface coating protection of semiconductor vacuum chamber. (b) Compared the corrosion characterization in 3.5 wt. % NaCl solution between naked AA6061 and AA6061 with Y 2 O 3 coating, a high corrosion reactivity ( $\mathrm{I}_{\text{corr}}=10^{-3.3}\mathrm{A}/\text{cm}^{2}$ ) of naked AA6061 then AA6061 with Y 2 O 3 coating ( $\mathrm{I}_{\text{corr}}=10^{-5.8}\mathrm{A}/\text{cm}^{2}$ ). The corrosion reactivity of AA6061 with Y 2 O 3 is reduced by 316 times to a naked AA6061.

关键词： Resistance Technological innovation Powders Image analysis Corrosion Semiconductor device manufacture Thermal spraying

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Fabrication of Low-Temperature Solid Oxide Fuel Cells via Development of Magnetron Sputtering Process

Fabrication of Low-Temperature Solid Oxide Fuel Cells via De...

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243rd ECS Meeting

作者： Ryu, Sangbong Yu, Wonjong Tran, Quang Tuyen Lee, Sanghoon Thieu, Cam Anh Sreenarayanan, Bhagath Feng, Shijie Meng, Shirley Fullerton, Eric Liu, Ping Minh, Nguyen Center for Energy Research University of California La Jolla San Diego92093 United States Department of NanoEngineering University of California La Jolla San Diego92093 United States Materials Science and Engineering University of California La Jolla San DiegoCA92093 United States Center for Memory and Recording Research University of California La Jolla San Diego92093 United States

ISBN: (纸本)9781607685395

We herein report the development of the sputtering process for the fabrication of low-temperature thin-film solid oxide fuel cells (TF-SOFCs) supported by anodized aluminum oxide (AAO). The process development has been conducted for each component, anode, electrolyte, electrolyte/cathode interlayer, and cathode to scale up the cell from 1cm x 1cm to 15 cm x 15 cm. In order to fabricate a uniform thin film regardless of the size of AAO, sputtering conditions such as power, pressure, angle, target-to-substrate distance (TSD), and time have been developed. The recipe for the anode and cathode has aimed at a porous structure with nano-grains for high reactivity. In contrast, the recipe for the electrolyte has been optimized for thickness uniformity and high density. With these sputtering recipes TF-SOFCs with the required microstructure and thickness uniformity have been successfully fabricated on 15 cm x 15 cm AAO substrates. © The Electrochemical Society.

关键词： Anodes

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Morphological Investigation and Ph Sensing Properties of β-Ga2O3 Egfet-Ph Sensor

SSRN

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SSRN 2023年

作者： Wuu, Dong-Sing Yadlapalli, Bharath Kumar Chou, Hsin-Yu Chiang, Jung-Lung Department of Materials Science and Engineering National Chung Hsing University Taichung40227 Taiwan Ph.D. Program Prospective Technology of Electrical Engineering and Computer Science National Chin-Yi University of Technology Taichung411030 Taiwan Department of Applied Materials and Optoelectronic Engineering National Chi Nan University Nantou54561 Taiwan

In our study, β-Ga2O3 particles of various morphologies deposited on FTO glass substrates through hydrothermal method under different pH conditions and pre-stirring temperatures. The particles examined using XRD, FESEM, FTIR and Raman spectroscopy instruments. The results showed that the β-Ga2O3 microrods prepared with pH=3.15 solution at different pre-stirring temperatures followed by hydrothermal reaction had several crystalline orientations ([−401], [002], [403]). The FTIR and Raman spectra measurements confirmed the existence of Ga−O−Ga and Ga−OH bending modes. In the range of pH=3-11, the EGFET-pH sensor prepared with β-Ga2O3 microrods shown a high current-pH sensitivity of 0.541 (mA)1/2 /pH with a linearity of 97.83 % and a high voltage-pH sensitivity of 73.99 mV/pH with linearity of 96.85 %. These results suggest that β-Ga2O3 microrods have pH sensitivity higher than the ideal Nernstian response. This confirms the use of β-Ga2O3 microrods as a sensing membrane in high-performance pH sensors or biosensors. © 2023, The Authors. All rights reserved.

关键词： Gallium compounds

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Ga2O3 nanorods synthesized by hydrothermal method for dual-functional sensing of pH value and glucose

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Sensors and Actuators A: Physical 2023年 363卷

作者： Chou, Hsin-Yu Yadlapalli, Bharath Kumar Yen, Chao-Chun Singh, Anoop Kumar Chiang, Jung-Lung Wuu, Dong-Sing Department of Materials Science and Engineering National Chung Hsing University Taichung40227 Taiwan Ph.D. Program Prospective Technology of Electrical Engineering and Computer Science National Chin-Yi University of Technology Taichung411030 Taiwan Department of Applied Materials and Optoelectronic Engineering National Chi Nan University Nantou54561 Taiwan

In this work, hydrothermal (HT)-synthesized gallium oxide (Ga2O3) nanorods were applied on indium tin oxide (ITO) glass substrates to create a dual-functional pH-glucose sensing device. Extended-gate field-effect transistors (EGFETs) were designed for pH and glucose sensing, and the response to pH levels ranging from 1 to 11 was tested using both chemical bath deposition (CBD) and high-pressure hydrothermal methods. Two sensor configurations, Ga2O3(CBD)/ITO/glass and Ga2O3(HT)/ITO/glass, were immersed in solution pH1 to 11 and cultured medium for hydrogen ion and glucose sensing. The results demonstrated that, for pH sensing in different samples, both IDS-VDS analysis and IDS-VGS analysis showed superior linearity and sensitivity with the HT method. For glucose detection, the HT method was selected for further experiments due to its maintained concentration-dependent relationship and selectivity towards various common sugars. When using culture medium DMEM as the sample (4.5 mg/ml glucose concentration), the accuracy of glucose estimation reached 99%. Stability and biocompatibility tests were also conducted, and the results indicated consistent performance. In summary, the HT-synthesized Ga2O3 nanorods exhibited high sensitivity, selectivity, and stability without affecting cell growth. Therefore, they hold great potential for applications not only in general blood glucose sensing but also in studying cell microenvironment metabolism. © 2023 Elsevier B.V.

关键词： Nanorods

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Injury and obesity differentially and synergistically induce dysregulation of synovial immune cells in osteoarthritis

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Annals of the Rheumatic Diseases 2025年第6期84卷 1033-1044页

作者： Harasymowicz, Natalia S. Harissa, Zainab Rashidi, Neda Lenz, Kristin Tang, Ruhang Guilak, Farshid Department of Orthopaedic Surgery Washington University St. Louis MO United States Shriners Hospitals for Children St. Louis MO United States Center of Regenerative Medicine Washington University St. Louis MO United States Department of Orthopaedic Surgery Operations University of Utah Salt Lake City UT United States Molecular Medicine Program University of Utah Salt Lake City UT United States Department of Biomedical Engineering Washington University in Saint Louis St. Louis MO United States Department of Mechanical Engineering & Materials Science Washington University St. Louis MO United States

Objectives: The heterogeneity and phenotype of immune cells orchestrate many physiologic and pathologic processes. Recent evidence suggests that immune cells play critical roles in the progression of osteoarthritis (OA). We hypothesised that injury and obesity, two major risk factors for OA, affect the immunophenotype of the synovium, the primary reservoir of immune cells in the joint. Methods: Using single-cell transcriptomics, immunoprofiling, transgenic mouse models, and genetic fate mapping methods, we characterised the presence and fate of multiple populations of immune cells found in the knee joint capsule. Results: We found that joint injury and obesity differentially and synergistically alter the architectural, cellular, and molecular profiles of the synovial capsule. We observed fewer patrolling monocytes in obese animals and found a significantly higher influx of proinflammatory monocyte-derived macrophages in the first 3 days after joint injury in obese compared with that in control animals. We also showed a significant loss of barrier-forming synovial lining macrophages 3 days after destabilisation of medial meniscus surgery, with a significant restoration of their numbers in normal weight but not in obese mice in advanced stages of OA. Finally, we characterised the presence and changes of other immune cell subtypes, including T, B, and mast cells and neutrophils, as well as local synovial fluid cytokines associated with injury and obesity. Conclusions: Our data revealed that injury and obesity independently and synergistically contribute to the dysregulation of the synovial immune landscape, providing new insight into their role in the pathogenesis of OA. © 2025 The Authors

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Understanding the effect of curvature on the magnetization reversal of three-dimensional nanohelices

arXiv

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arXiv 2023年

作者： Fullerton, John McCray, Arthur R.C. Petford-Long, Amanda K. Phatak, Charudatta Materials Science Division Argonne National Laboratory LemontIL United States Applied Physics Program Northwestern University EvanstonIL United States Department of Materials Science and Engineering Northwestern University EvanstonIL United States

Comprehending the interaction between geometry and magnetism in three-dimensional (3D) nanostructures is of importance to understand the fundamental physics of domain wall (DW) formation and pinning. Here, we use focused electron beam-induced deposition to fabricate magnetic nanohelices with increasing helical curvature with height. Using electron tomography and Lorentz transmission electron microscopy, we reconstruct the 3D structure and magnetization of the nanohelices. The surface curvature, helical curvature and torsion of the nanohelices are then quantified from the tomographic reconstructions. Furthermore, by using the experimental 3D reconstructions as inputs for micromagnetic simulations we can reveal the influence of surface and helical curvature on the magnetic reversal mechanism. Hence, we can directly correlate the magnetic behavior of a 3D nanohelix to its experimental structure. These results demonstrate how control of geometry in nanohelices can be utilized in the stabilization of DWs and control of the response of the nanostructure to applied magnetic fields. Copyright © 2023, The Authors. All rights reserved.

关键词： Magnetization reversal

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Versatile Organic Electrochemical Transistors with Self-Assembled Coronene Nanofiber Arrays for the Isolation and Detection of Circulating Tumor Cells and Enhanced Secretion of Extracellular Vesicles

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ACS applied materials & interfaces 2025年第23期17卷 2025 May 30页

作者： Edgar Daniel Quiñones Jiashing Yu Rou-Zhen Liu Yi-Shiuan Li Yu-Chuan Lu Yu-Sheng Hsiao Department of Materials Science and Engineering National Taiwan University of Science and Technology Taipei 106335 Taiwan. Taiwan International Graduate Program Sustainable Chemical Science & Technology Academia Sinica Institute of Chemistry Taipei 115 Taiwan. Department of Chemical Engineering National Taiwan University Taipei 10617 Taiwan. Department of Materials Engineering Ming Chi University of Technology New Taipei City 24301 Taiwan. Department of Surgical Oncology National Taiwan University Cancer Center National Taiwan University College of Medicine Taipei 10672 Taiwan. Department of Urology National Taiwan University Hospital Taipei 100225 Taiwan. Energy and Sustainability Tech Graduate Institute National Taiwan University of Science and Technology Taipei 106335 Taiwan.

The integration of organic bioelectronic interfaces has opened new avenues for merging biological systems with electronics, offering transformative solutions to challenges in both fields. In this study, we present a novel hybrid organic electrochemical transistor (OECT) featuring self-assembled three-dimensional (3D) nanofiber arrays (NFAs) of small-molecule semiconductors grown on poly(3,4-ethylenedioxythiophene):polystyrenesulfonate active-layer channels. The hybrid OECT was further evaluated for its potential to capture, recover, and biosense circulating tumor cells (CTCs), as well as to enhance extracellular vesicle (EV) secretion through electrical stimulation (ES). Using template-free self-assembly via standard thermal evaporation, out-of-plane coronene (CR)-based NFAs were fabricated and surface-engineered with 1-pyrenebutyric acid through π-π interactions, enabling bioaffinity coatings on OECTs for advanced biological applications. The device demonstrated efficient CTC isolation, achieving an isolation rate exceeding 90% for MCF-7 breast cancer cells spiked into THP-1 monocytic cells (10 cells mL), with minimal nonspecific binding by incorporating a specific antibody-coated CR-based NFA layer. Moreover, over 80% of the captured CTCs were released from CR-based NFAs during cyclic voltammetry sweeps in phosphate-buffered saline. The device also enhanced EV secretion by incorporating a collagen-coated CR-based NFA layer, which supported cell attachment and proliferation under sustained ES at 20 V, 0.5 Hz, with 5 ms pulses for 24-72 h periods. EV production increased by approximately 12.4-fold in MCF-7 cells and 8.0-fold in immortalized bone marrow stromal cells without significantly altering the EV size or requiring additional cellular modifications. This dual-functionality platform, enabled by a surface-engineered 3D-hybrid OECT, is a powerful tool for selective CTC isolation, liquid biopsy purification, and enhanced EV production. This versatility highlights

关键词： bioelectronic interfaces (BEIs) circulating tumor cells (CTCs) extracellular vesicle (EV) nanofiber arrays (NFAs) organic electrochemical transistor (OECT) poly(3,4-ethylenedioxythiophene):polystyrenesulfonate (PEDOT:PSS)

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