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Performance Enhancement of Electrochemiluminescence with the Immunosensor Controlled Using Magnetized Masks for the Determination of Epithelial Cancer Biomarker EpCAM

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Analytical Chemistry 2023年第2期95卷 986-993页

作者： Huang, Chun-Yao Lin, Feng-Yen Chang, Chi-Jung Lu, Chien-Hsing Chen, Jem-Kun Taipei Heart Institute Taipei Medical University 250 Wu-Hsing Street Taipei110 Taiwan Division of Cardiology and Cardiovascular Research Center Taipei Medical University Hospital 252 Wu-Hsing Street Taipei110 Taiwan Department of Internal Medicine School of Medicine College of Medicine Taipei Medical University 250 Wu-Hsing Street Taipei110 Taiwan Department of Biomedical Sciences and Engineering National Central University 300 Zhongda Road Taoyuan City320317 Taiwan Department of Materials Science and Engineering National Taiwan University of Science and Technology 43 Sec. 4 Keelung Road Taipei106 Taiwan Department of Chemical Engineering Feng Chia University 100 Wenhwa Road Taichung40724 Taiwan Department of Obstetrics and Gynecology Taichung Veterans General Hospital 1650 Taiwan Boulevard Sect. 4 Taichung40705 Taiwan Institute of Biomedical Sciences Ph.D. Program in Translational Medicine Rong-Hsing Research Center for Translational Medicine National Chung-Hsing University Taichung940227 Taiwan

The performance of an electrochemiluminescence (ECL) immunosensor was improved with a particle gradient. SiO2-coated magnetic beads were adopted as nanocarriers for gradient manipulation and immobilized with the primary antibody. Cadmium telluride quantum dots were coated with a layer of protein G for conjugation and orientation of the secondary antibody as signal labels. ECL immunosensor gradients on the electrode were formed by magnetolithography (ML) with magnetized nickel masks of column and stripe arrays. The immunosensor generally aggregated as an island on the substrate, leading to a decrease of efficiency in the characteristic signals. Stripe arrays of magnetized nickel were designed to generate cylindrical magnetic flux on the substrate to improve the particle manipulation with the gradient. Various gradients of the sandwich-structured immunosensor substantially affected the electrochemical performance. Compared to the gradient-free immunosensor, the gradient of the immunosensor generated by ML using a 3 μm stripe array mask enhanced the ECL intensity ∼2.2 times. The results of quantification of epithelial cell adhesion molecules (EpCAM) with the gradient immunosensor showed a broad linear range (15-420 pg mL-1), a low limit of detection (5.5 pg mL-1), and high reliability for EpCAM-spiked serum samples, indicating that the immunosensor gradient substantially enhances the performance of the ECL assay. © 2022 American Chemical Society.

关键词： Immunosensors

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Concurrent Light-Based Motor Mapping of Multiple Limbs Using Deep-Learning-Driven Pose Estimation

Concurrent Light-Based Motor Mapping of Multiple Limbs Using...

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Optics and the Brain, BRAIN 2024 - Part of Optica Biophotonics Congress: biomedical Optics

作者： Khanal, Nischal Padawer-Curry, Jonah Bice, Annie R. Bauer, Adam Q. Mallinckrodt Institute of Radiology Washington University School of Medicine in St. Louis Washington University in St. Louis St. LouisMO United States Department of Biomedical Engineering Washington University in St. Louis St. LouisMO United States Imaging Science Program Washington University in St. Louis St. LouisMO United States

Light-based motor mapping and DeepLabCut (DLC-LBMM) were used to map cortical representations of multiple limbs concurrently in 3D. Motor representations of fine, articulated movements, and multi-limb movement traject... 详细信息

ISBN: (纸本)9781957171340

关键词： Mapping

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Improving Mechanical Durability of SLA-Printed Components for Load-Bearing 154th

Improving Mechanical Durability of SLA-Printed Components fo...

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154th Annual Meeting and Exhibition of The Minerals, Metals and Materials Society, TMS 2025

作者： Fani, Niloofar Monfared, Armaghan Hashemi Sadeghzade, Sorour Tavangarian, Fariborz Mechanical Engineering Program School of Science Engineering and Technology Pennsylvania State University Harrisburg MiddletownPA17057 United States Department of Biomedical Engineering Pennsylvania State University State College University ParkPA16802 United States School of Engineering Westlake University Hangzhou310024 China

ISBN: (纸本)9783031807473

This study explored the impact of post-printing parameters in stereolithography (SLA) technology, particularly focusing on how photopolymerization affected the mechanical properties of samples. Building on previous research, the investigation revealed that different curing temperatures significantly influenced the rigidity and stiffness of the printed samples. The mechanical properties of the samples were evaluated through three-point bending tests. Curing at 60 °C left some polymer chains uncured, resulting in less rigid samples but increased load capacity (up to 30 ± 2.4 N) and deflection (2.9 ± 0.6 mm). On the other hand, curing at 70 °C enhanced the rigidity and stiffness, especially for larger samples. The results demonstrated that photopolymerization and the resulting polymer chain cross-linking are crucial for achieving the desired mechanical properties. Notably, as the diameter of the samples decreased, the difference in stiffness between the 60 and 70 °C cured samples also reduced. The findings highlight the importance of optimizing curing temperatures to tailor the mechanical performance of SLA-printed components for specific applications. © The Minerals, Metals & Materials Society 2025.

关键词： Bending tests

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Utilizing Low-Dimensional Molecular Embeddings for Rapid Chemical Similarity Search

arXiv

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arXiv 2024年

作者： Kirchoff, Kathryn E. Wellnitz, James Hochuli, Joshua E. Maxfield, Travis Popov, Konstantin I. Gomez, Shawn Tropsha, Alexander Department of Computer Science UNC Chapel Hill United States Eshelman School of Pharmacy UNC Chapel Hill United States Department of Pharmacology UNC Chapel Hill United States Joint Department of Biomedical Engineering UNC Chapel Hill NCSU United States

Nearest neighbor-based similarity searching is a common task in chemistry, with notable use cases in drug discovery. Yet, some of the most commonly used approaches for this task still leverage a brute-force approach. In practice this can be computationally costly and overly time-consuming, due in part to the sheer size of modern chemical databases. Previous computational advancements for this task have generally relied on improvements to hardware or dataset-specific tricks that lack generalizability. Approaches that leverage lower-complexity searching algorithms remain relatively underexplored. However, many of these algorithms are approximate solutions and/or struggle with typical high-dimensional chemical embeddings. Here we evaluate whether a combination of low-dimensional chemical embeddings and a k-d tree data structure can achieve fast nearest neighbor queries while maintaining performance on standard chemical similarity search benchmarks. We examine different dimensionality reductions of standard chemical embeddings as well as a learned, structurally-aware embedding-SmallSA-for this task. With this framework, searches on over one billion chemicals execute in less than a second on a single CPU core, five orders of magnitude faster than the brute-force approach. We also demonstrate that SmallSA achieves competitive performance on chemical similarity benchmarks. © 2024, CC BY.

关键词： Embeddings

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Injectable cross-linked hyaluronic acid hydrogels with epigallocatechin gallate loading as vitreous substitutes

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International Journal of Biological Macromolecules 2024年第Part1期275卷 133467页

作者： Chen, Huai-An Tai, Yi-Ning Hsieh, Erh-Hsuan Thacker, Minal Lin, I-Chan Tseng, Ching-Li Lin, Feng-Huei Graduate Institute of Biomedical Materials and Tissue Engineering College of Biomedical Engineering Taipei Medical University Shuang-Ho Campus New Taipei City Taiwan Institute of Biomedical Engineering College of Medicine & College of Engineering National Taiwan University Taipei City Taiwan 17W Hong Kong Science Park Hong Kong Department of Ophthalmology Wan Fang Hospital Taipei Medical University Taipei City Taiwan Department of Ophthalmology School of Medicine College of Medicine Taipei Medical University Taipei City Taiwan International Ph. D. Program in Biomedical Engineering College of Biomedical Engineering Taipei Medical University Shuang-Ho Campus New Taipei City Taiwan Research Center of Biomedical Device College of Biomedical Engineering Taipei Medical University Taipei City Taiwan International Ph. D. Program in Cell Therapy and Regenerative Medicine College of Medicine Taipei Medical University Taipei City Taiwan National Health Research Institutes Miaoli County Taiwan

Hyaluronic acid (HA) serves as a vitreous substitute owing to its ability to mimic the physical functions of native vitreous humor. However, pure HA hydrogels alone do not provide sufficient protection against potential inflammatory risks following vitrectomy. In this study, HA was crosslinked with 1,4-butanediol diglycidyl ether (BDDE) to form HA hydrogels (HB). Subsequently, the anti-inflammatory agent epigallocatechin gallate (EGCG) was added to the hydrogel (HBE) for ophthalmic applications as a vitreous substitute. The characterization results indicated the successful preparation of HB with transparency, refractive index, and osmolality similar to those of native vitreous humor, and with good injectability. The anti-inflammatory ability of HBE was also confirmed by the reduced expression of inflammatory genes in retinal pigment epithelial cells treated with HBE compared with those treated with HB. In a New Zealand white rabbit model undergoing vitreous substitution treatment, HBE 50 (EGCG 50 μM addition) exhibited positive results at 28 days post-surgery. These outcomes included restored intraocular pressure, improved electroretinogram responses, minimal increase in corneal thickness, and no inflammation during histological examination. This study demonstrated the potential of an injectable HA-BDDE cross-linked hydrogel containing EGCG as a vitreous substitute for vitrectomy applications, offering prolonged degradation time and anti-inflammatory effects postoperatively. © 2024 The Authors

关键词： Hyaluronic acid

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Revolutionizing Neurological Diagnostics: Integrating 6G Technology with Deep Learning for Enhanced Detection of Multiple Sclerosis and Myelitis

Revolutionizing Neurological Diagnostics: Integrating 6G Tec...

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Telecommunications Conference (ITC-Egypt), International

作者： Rana M. NourEldeen Rahma A. Elshshtawy Omnia A. Elgohary Fatma Alzahraa A. Wahba Marco K. Attia Zeinab B. Abo elazm Mohamed G. Khattap Hend Galal Eldeen Mohamed Ali Hassan Mariam Mostafa Nayra Ibrahim Reem Ramadan Yomna Refaat Mennatallah Khaled Ali Maher Mohamed Abd Elaziz Ahmed Gamal Abdellatif Ibrahim Radiology and Medical Imaging Faculty of Applied Health Sciences Galala University Suez Egypt Biomedical Informatics Faculty of Computer Science and Engineering Galala University Suez Egypt Artificial Intelligence Science Program Faculty of Computer Science and Engineering Galala University Suez Egypt Department of Electronics and Electrical Communications Engineering Air Defense College Alexandria Egypt

ISBN: (数字)9798350351408

ISBN: (纸本)9798350351415

This paper proposes an alternative detection frame-work for multiple sclerosis (MS) and idiopathic acute transverse myelitis (ATM) within the 6G-enabled Internet of Medical Things (IoMT) environment. The developed framework relies on the implementation of a deep learning technique known as Dense Convolutional Networks (DenseNets) in the 6G-enabled IoMT to enhance prediction performance. To validate the performance of DenseNets, we compared it with other deep learning techniques, including Convolutional Neural Networks (CNN) and MobileNet, using real-world datasets. The experimental results show the high performance of DenseNets in predicting MS and ATM compared to other methods, achieving an accuracy of nearly 90 %.

关键词： 6G mobile communication Deep learning Multiple sclerosis Accuracy Internet of Medical Things Telecommunications Convolutional neural networks

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Salacca zalacca extract's antiaging effect on aging genes, protein levels, and apoptosis in UV-induced fibroblast cells

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Journal of Taibah University Medical sciences 2025年第3期20卷 349-358页

作者： Widowati, Wahyu Dani, Dani Vera, Vera Wargasetia, Teresa L. Rahardja, Fanny Tih, Fen Onggowidjaja, Philips Tjokropranoto, Rita Zahiroh, Fadhilah H. Azis, Rizal Priyandoko, Didik Surakusumah, Wahyu Hadiprasetyo, Dhanar S. Faculty of Medicine Maranatha Christian University West Java Bandung Indonesia Bimolecular and Biomedical Research Center Aretha Medika Utama West Java Bandung Indonesia Biomedical Engineering Department of Electrical Engineering Faculty of Engineering Universitas Indonesia West Java Depok Indonesia Biology Study Program Faculty of Mathematics and Science Education Universitas Pendidikan Indonesia West Java Bandung Indonesia Faculty of Pharmacy Universitas Jenderal Achmad Yani West Java Cimahi Indonesia

Objectives: Ultraviolet (UV) exposure can hasten the aging process of the skin. The use of chemicals for anti-aging has long-term adverse effects. The natural ingredients of snake fruit (Salacca zalacca L.) are known to have bioactive properties such as polyphenols, flavonoids, chlorogenic acid, and caffeic acid which have antiaging potential. The study aims to ascertain the potential of S. zalacca Extract (SZE) as an antiaging agent by in vitro assay. Methods: The SZE compound content was analyzed by LC-MS/MS. SZE viability test on human skin fibroblast (BJ) cells was carried out using the WST assay. BJ cells were UV-induced as a cell model of premature aging. SZE 6.25, 12.5, and 25 μg/mL were administered to UV-induced BJ cells. The gene expression of COL1A1, MMP-1, FGF-2, and GPX-1 were analyzed by quantitative Real-Time PCR. Elastin (ELN), Hyaluronidase (HAse), Cyclooxigenase-2 (COX-2), 8-Hydroxydeoxyguanosine (8-OHdG), and Melatonin (MT) protein levels were analyzed by ELISA assay. The apoptosis of BJ cells was analyzed using flow cytometry. One-way ANOVA in SPSS Software was used for statistical analysis. Results: Treatment with SZE increased COL1A1, FGF-2, and GPX-1 gene expression and also decreased MMP-1 gene expression. SZE also increased ELN and MT levels in UV-induced BJ cells. After SZE treatment, the protein levels of HAse, COX-2, and 8-OHdG decreased compared to the positive control. SZE also succeeded in maintaining the lives of BJ cells and reducing apoptosis in BJ cells. Conclusions: SZE has the potential to be an antiaging agent by in vitro assay. © 2025 The Authors

关键词： Antiaging Antioxidant Chlorogenic acid Salacca zalacca Ultraviolet

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Mussel-Inspired and Bioclickable Peptide Engineered Surface to Combat Thrombosis and Infection

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Research 2022年第3期2022卷 91-104页

作者： Xiaohui Mou Hongbo Zhang Hua Qiu Wentai Zhang Ying Wang Kaiqin Xiong Nan Huang Hélder A.Santos Zhilu Yang Affiliated Dongguan Hospital Southern Medical UniversityDongguanGuangdong 523059China Guangdong Provincial Key Laboratory of Shock and Microcirculation GuangzhouGuangdong 510080China Key Laboratory of Advanced Technologies of Materials Ministry of EducationSchool of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031China Pharmaceutical Sciences Laboratory Åbo Akademi UniversityTurku Biosciences CenterUniversity of Turku andÅbo Akademi University20520 TurkuFinland State Key Laboratory of Molecular Engineering of Polymers Fudan UniversityShanghai 200438China Department of Biomedical Engineering and W.J.Kolff Institute for Biomedical Engineering and Materials Science University Medical Center Groningen/University of GroningenAnt.Deusinglaan 19713 AV GroningenNetherlands Drug Research Program Division of Pharmaceutical Chemistry and TechnologyFaculty of PharmacyUniversity of HelsinkiFI-00014 HelsinkiFinland

Thrombosis and infections are the two major complications associated with extracorporeal circuits and indwelling medical devices,leading to significant mortality in *** address this issue,here,we report a biomimetic surface engineering strategy by the integration of mussel-inspired adhesive peptide,with bio-orthogonal click chemistry,to tailor the surface functionalities of tubing and *** by mussel adhesive foot protein,a bioclickable peptide mimic(DOPA)4-azidebased structure is designed and grafted on an aminated tubing robustly based on catechol-amine ***,the dibenzylcyclooctyne(DBCO)modified nitric oxide generating species of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid(DOTA)chelated copper ions and the DBCO-modified antimicrobial peptide(DBCO-AMP)are clicked onto the grafted surfaces via bio-orthogonal *** combination of the robustly grafted AMP and Cu-DOTA endows the modified tubing with durable antimicrobial properties and ability in long-term catalytically generating NO from endogenous snitrosothiols to resist adhesion/activation of platelets,thus preventing the formation of ***,this biomimetic surface engineering technology provides a promising solution for multicomponent surface functionalization and the surface bioengineering of biomedical devices with enhanced clinical performance.

关键词： DOTA generating Engine

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Enhancing Acute Ischemic Stroke Diagnosis Using IoMT and Deep Learning Technologies

Enhancing Acute Ischemic Stroke Diagnosis Using IoMT and Dee...

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Telecommunications Conference (ITC-Egypt), International

作者： Mohamed M. El-Sheikh Aya Bayoumy Nada M. Ahmed Amany Mohamed Eldeeb Fatma I. Rabea Amira A. Nawar Mohamed G. Khattap Hend Galal Eldeen Mohamed Ali Hassan Mariam Mostafa Nayra Ibrahim Reem Ramadan Yomna Refaat Mennatallah Khaled Ali Maher Mohamed Abd Elaziz Ahmed Gamal Abdellatif Ibrahim Radiology and Medical Imaging Faculty of Applied Health Sciences Galala University Suez Egypt Biomedical Informatics Faculty of Computer Science and Engineering Galala University Suez Egypt Artificial Intelligence Science Program Faculty of Computer Science and Engineering Galala University Suez Egypt Department of Electronics and Electrical Communications Engineering Air Defense College Alexandria Egypt

ISBN: (数字)9798350351408

ISBN: (纸本)9798350351415

This paper introduces an alternative technique for diagnosing Acute Ischemic Stroke within the IoMT environment. In the proposed approach, the collected data is transmitted to a cloud-based center where the technique utilizes EfficientNet, a deep learning model, designed to extract features from MRI images thereby enhancing the detection of acute ischemic infarctions. The performance of EfficientNet is compared against two other models, CNN and MobileNet, demonstrating its superior efficacy through metrics such as accuracy, precision, recall, and F1-score, which stand at 92.31%, 92.28%, 92.33%, and 92.30%, respectively.

关键词： Deep learning Accuracy Magnetic resonance imaging Soft sensors Medical services Feature extraction Regulation

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The roles of bone remodeling in normal hematopoiesis and age-related hematological malignancies

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Bone Research 2023年第2期11卷 273-291页

作者： Hengwei Zhang Jane LLiesveld Laura MCalvi Brea CLipe Lianping Xing Michael WBecker Edward MSchwarz Shu-Chi A.Yeh Center for Musculoskeletal Research University of Rochester Medical Center601 Elmwood AveBox 665RochesterNY14642USA Department of Pathology and Laboratory Medicine University of Rochester Medical CenterRochesterNYUSA Wilmot Cancer Center University of Rochester Medical CenterRochesterNYUSA Department of Medicine Division of Hematology/Oncology and Bone Marrow Transplantation ProgramUniversity of Rochester Medical CenterRochesterNYUSA Department of Medicine Division of Endocrinology/MetabolismUniversity of Rochester Medical CenterRochesterNYUSA Department of Orthopaedics University of Rochester Medical CenterRochesterNYUSA Department of Medicine Division of Allergy/Immunology/RheumatologyUniversity of Rochester Medical CenterRochesterNYUSA Department of Biomedical Engineering University of RochesterNYUSA Department of Physiology/Pharmacology University of Rochester Medical CenterRochesterNYUSA

Prior research establishing that bone interacts in coordination with the bone marrow microenvironment(BMME)to regulate hematopoietic homeostasis was largely based on analyses of individual bone-associated cell *** advances in intravital imaging has suggested that the expansion of hematopoietic stem cells(HSCs)and acute myeloid leukemia cells is restricted to bone marrow microdomains during a distinct stage of bone *** findings indicate that dynamic bone remodeling likely imposes additional heterogeneity within the BMME to yield differential clonal responses.A holistic understanding of the role of bone remodeling in regulating the stem cell niche and how these interactions are altered in age-related hematological malignancies will be critical to the development of novel *** advance this understanding,herein,we provide a synopsis of the cellular and molecular constituents that participate in bone turnover and their known connections to the hematopoietic ***,we elaborate on the coupling between bone remodeling and the BMME in homeostasis and age-related hematological malignancies and after treatment with bone-targeting *** then discuss unresolved questions and ambiguities that remain in the field.

关键词： remodeling homeostasis likely

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