In this paper, we have formulated and studied a stage-structure predator-prey model. Here, we consider stage-structure on both prey as well as predator population which means that the prey population is divided into t...
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In this paper, a delayed predator-prey model has been developed. Here, on the basis of infectious disease, the prey population has been divided into two sub-populations such as (i) susceptible prey and (ii) infected p...
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Background & AimCRISPR/Cas9 technology is a leading method for efficient gene editing, enabling the creation of isogenic pairs of mutant and control human induced pluripotent stem cells (hiPSCs). While hiPSCs prov...
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Background & AimCRISPR/Cas9 technology is a leading method for efficient gene editing, enabling the creation of isogenic pairs of mutant and control human induced pluripotent stem cells (hiPSCs). While hiPSCs provide valuable opportunities for disease modeling and personalized medicine, challenges remain in distinguishing specific mutation effects from genetic backgrounds. Manual genome editing processes often face scalability and standardization limitations. The StemCellFactory, a modular robotic system, has been developed to automate the reprogramming, expansion, and differentiation of hiPSCs, enhancing the application of CRISPR/Cas9 in research. MethodologyHiPSCs were cultured in StemMACS iPS-Brew XF medium, with daily medium changes and mycoplasma testing. For CRISPR/Cas9 editing, target gene-specific CRISPR-RNA and tracrRNA formed ribonucleoprotein complexes, utilizing an optimized nucleofection protocol. HiPSCs were dissociated, counted, and nucleofected through manual and automated processes. The automated StemCellFactory workflow included cell preparation, nucleofection, and cell seeding, managed by the COPE software for data visualization. Genotyping of polyclonal hiPSCs was performed via Sanger sequencing to assess editing efficiency, with automated isolation of monoclonal colonies conducted using an integrated CellCelector. ResultsThe StemCellFactory successfully integrated a 4D-Nucleofector with a 96-well shuttle device for automated CRISPR/Cas9 editing. Adjustments replaced manual consumables with scalable formats, enhancing efficiency. The CM150 electroporation program yielded high cell viability (83.5%) and indel rates (81.7%). Optimal culture conditions combined Laminin 521 with CloneR-supplemented iPS-Brew medium, supporting hiPSC survival and colony formation. The automated system allowed parallel processing of up to 24 conditions, demonstrating high editing efficiencies, with indel rates exceeding 80% across all tested cell lines, comparable to ma
Formal methods (in a broad sense) have been around almost since the beginning of computer science. Nonetheless, there is a perception in the formal methods community that take-up by industry is low considering the pot...
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Let σ = {σi|i ∈ I} be a partition of the set P of all primes and G a finite group. A chief factor H/K of G is said to be σ-central if the semidirect product (H/K) (G/CG(H/K)) is a σi-group for some i = i(H/K). G ...
This work presents results of computer simulation of a flat reflective array of rectangular waveguides and results of the investigation of the influence of phase errors on the formation of the radiation field.
This work presents results of computer simulation of a flat reflective array of rectangular waveguides and results of the investigation of the influence of phase errors on the formation of the radiation field.
Throughout this paper, all groups are finite. Let σ- = -σ-i|i ∈ I} be some partition of the set of all primes P. If n is an integer, the symbol σ-(n) denotes the set -σ-i|σ-i ∩ π(n) ≠ ø.}. The integers n...
The paper proposes the architecture of distributed multilevel detection system of malicious software in local area networks. Its feature is the synthesis of its requirements of distribution, decentralization, multilev...
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The paper proposes the architecture of distributed multilevel detection system of malicious software in local area networks. Its feature is the synthesis of its requirements of distribution, decentralization, multilevel. This allows you to use it autonomously. In addition, the feature of autonomous program modules of the system is the same organization, which allows the exchange of knowledge in the middle of the system, which, unlike the known systems, allows you to use the knowledge gained by separate parts of the system in other parts. The developed system allows to fill it with subsystems of detection of various types of malicious software in local area networks.
A numerical study on the flow over a square cylinder in the vicinity of a wall is conducted for different Couette-Poiseuille-based non-uniform flow with the non-dimensional pressure gradient P varying from 0 to 5. The...
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