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Single-shot Quantitative Gradient Phase Microscopy (QGPM) to Monitor Cellular Kinetics

Microscopy and Microanalysis 2024年第SUPPLEMENT_1期30卷

作者： Mohammadi, Melika Haji Hur, Sun Woong Kwon, Minsung Samuel, Ashok Zachariah Bhargava, Rohit Department of Bioengineering University of Illinois Urbana-Champaign Urbana IL United States Beckman Institute for Advanced Science and Technology Urbana IL United States Departments of Chemical and Biomolecular Engineering Electrical and Computer Engineering Mechanical Science and Engineering and Chemistry Cancer Center at Illinois University of Illinois Urbana-Champaign Urbana IL United States

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Edge Selections in Bilinear Dynamic Networks

arXiv

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arXiv 2020年

作者： de Oliveira, Arthur Castello B. Siami, Milad Sontag, Eduardo D. Department of Electrical & Computer Engineering Northeastern University BostonMA02115 United States Departments of Bioengineering Northeastern University BostonMA02115 United States

We develop some basic principles for the design and robustness analysis of a continuous-time bilinear dynamical network, where an attacker can manipulate the strength of the interconnections/edges between some of the agents/nodes. We formulate the edge protection optimization problem of picking a limited number of attack-free edges and minimizing the impact of the attack over the bilinear dynamical network. In particular, the H2-norm of bilinear systems is known to capture robustness and performance properties analogous to its linear counterpart and provides valuable insights for identifying which edges are most sensitive to attacks. The exact optimization problem is combinatorial in the number of edges, and brute-force approaches show poor scalability. However, we show that the H2-norm as a cost function is supermodular and, therefore, allows for efficient greedy approximations of the optimal solution. We illustrate and compare the effectiveness of our theoretical findings via numerical simulations. Copyright © 2020, The Authors. All rights reserved.

关键词： Cost functions

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Author Correction: Dynamical flexible inference of nonlinear latent factors and structures in neural population activity

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Nature biomedical engineering 2025年第2期9卷 283页

作者： Hamidreza Abbaspourazad Eray Erturk Bijan Pesaran Maryam M Shanechi Ming Hsieh Department of Electrical and Computer Engineering Viterbi School of Engineering University of Southern California Los Angeles CA USA. Departments of Neurosurgery Neuroscience and Bioengineering University of Pennsylvania Philadelphia PA USA. Ming Hsieh Department of Electrical and Computer Engineering Viterbi School of Engineering University of Southern California Los Angeles CA USA. shanechi@usc.edu. Thomas Lord Department of Computer Science Alfred E. Mann Department of Biomedical Engineering Neuroscience Graduate Program University of Southern California Los Angeles CA USA. shanechi@usc.edu.

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Polarimetric infrared spectroscopic imaging using quantum cascade lasers

Polarimetric infrared spectroscopic imaging using quantum ca...

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Advanced Chemical Microscopy for Life Science and Translational Medicine 2020

作者： Phal, Yamuna Yeh, Kevin Bhargava, Rohit Department of Electrical and Computer Engineering University of Illinois at Urbana-Champaign UrbanaIL United States Departments of Bioengineering Chemical and Biomolecular Engineering Mechanical Science and Engineering University of Illinois at Urbana-Champaign UrbanaIL United States Cancer Center at Illinois University of Illinois at Urbana-Champaign UrbanaIL United States 4265 Beckman Institute for Advanced Science and Technology University of Illinois at Urbana-Champaign 405 N. Mathews Avenue UrbanaIL61801 United States

ISBN: (纸本)9781510632677

Infrared spectroscopic imaging has emerged as a powerful label-free diagnostic tool to study the molecular composition and organization in biological tissues and cells. We report infrared spectroscopic imaging using polarized light to study differential absorption of plane-polarized light by an oriented sample to detect valuable information, such as, birefringence and dichroism. For instance, the organization of collagen, specifically fiber orientation and alignment, is crucial in understanding the progression and metastasis of cancer. Recent advancements in the development of Quantum Cascade Lasers (QCL) sources have opened new avenues for high SNR measurements in the field of IR spectroscopy. In addition, QCL sources are intrinsically polarized and orientation information can be obtained at discrete frequencies with different polarization orientations, allowing much faster acquisition than a corresponding FT-IR approach. We demonstrate improved performance in terms of fast and comprehensive polarimetric image acquisition and analysis using custom-built QCL microscope and evaluate its impact on applications by analyzing the important spectral bands of surgical tissue sections. © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

关键词： Collagen

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Data-driven sparse skin stimulation can convey social touch information to humans

arXiv

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arXiv 2021年

作者： Salvato, Mike Williams, Sophia R. Nunez, Cara M. Zhu, Xin Israr, Ali Lau, Frances Klumb, Keith Abnousi, Freddy Okamura, Allison M. Culbertson, Heather Department of Mechanical Engineering Stanford University StanfordCA94305 United States Department of Electrical Engineering Stanford University StanfordCA94305 United States Departments of Bioengineering and Mechanical Engineering Stanford University StanfordCA94305 United States Department of Computer Science University of Southern California Los AngelesCA90089 United States Facebook Inc. 1 Hacker Way Menlo ParkCA94025 United States

During social interactions, people use auditory, visual, and haptic cues to convey their thoughts, emotions, and intentions. Due to weight, energy, and other hardware constraints, it is difficult to create devices that completely capture the complexity of human touch. Here we explore whether a sparse representation of human touch is sufficient to convey social touch signals. To test this we collected a dataset of social touch interactions using a soft wearable pressure sensor array, developed an algorithm to map recorded data to an array of actuators, then applied our algorithm to create signals that drive an array of normal indentation actuators placed on the arm. Using this wearable, low-resolution, low-force device, we find that users are able to distinguish the intended social meaning, and compare performance to results based on direct human touch. As online communication becomes more prevalent, such systems to convey haptic signals could allow for improved distant socializing and empathetic remote human-human interaction. Copyright © 2021, The Authors. All rights reserved.

关键词： Actuators

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Analysis of 3D Collagen Organization in Non-pregnant Rat Cervix Tissue

Analysis of 3D Collagen Organization in Non-pregnant Rat Cer...

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Frontiers in Optics, FiO_2019

作者： Lee, Woowon Moghaddam, Amir Ostadi McFarlin, Barbara L. Wagoner Johnson, Amy J. Toussaint, Kimani C. University of Illinois at Urbana-Champaign Department of Mechanical Science and Engineering UrbanaIL United States University of Illinois at Chicago Department of Women Children and Family Health Science ChicagoIL United States University of Illinois at Urbana-Champaign Departments of Electrical and Computer Engineering and Bioengineering UrbanaIL United States

We quantitatively analyze non-pregnant rat cervix on three distinct regions using second-harmonic generation imaging. 3D structural parameters related to collagen organization are obtained on each region respectively.... 详细信息

ISBN: (纸本)9781943580675

关键词： Rats

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Construction and analysis of posterior matching in arbitrary dimensions via optimal transport

arXiv

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arXiv 2019年

作者： Mesa, Diego A. Ma, Rui Gorantla, Siva Coleman, Todd P. Departments of Biomedical Informatics Electrical Engineering and Computer Science Vanderbilt University Dexcomm Inc Google Inc Department of Bioengineering University of California San Diego

The posterior matching scheme, for feedback encoding of a message point lying on the unit interval over memoryless channels, maximizes mutual information for an arbitrary number of channel uses. However, it in general does not always achieve any positive rate;so far, elaborate analyses have been required to show that it achieves any positive rate below capacity. More recent efforts have introduced a random "dither" shared by the encoder and decoder to the problem formulation, to simplify analyses and guarantee that the randomized scheme achieves any rate below capacity. Motivated by applications (e.g. human-computer interfaces) where (a) common randomness shared by the encoder and decoder may not be feasible and (b) the message point lies in a higher dimensional space, we focus here on the original formulation without common randomness, and use optimal transport theory to generalize the scheme for a message point in a higher dimensional space. By defining a stricter, almost sure, notion of message decoding, we use classical probabilistic techniques (e.g. change of measure and martingale convergence) to establish succinct necessary and sufficient conditions on when the message point can be recovered from infinite observations: Birkhoff ergodicity of a random process sequentially generated by the encoder. We also show a surprising "all or nothing" result: the same ergodicity condition is necessary and sufficient to achieve any rate below capacity. We provide applications of this message point framework in human-computer interfaces and multi-antenna communications. Copyright © 2019, The Authors. All rights reserved.

关键词： Signal encoding

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A robust Lyapunov criterion for non-oscillatory behaviors in biological interaction networks

arXiv

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arXiv 2020年

作者： Angeli, David Al-Radhawi, M. Ali Sontag, Eduardo D. The Department of Electrical & Electronic Engineering Imperial College London LondonSW7 2AZ United Kingdom Dipartimento di Ingegneria dell'Informazione University of Florence Florence Italy Department of Electrical and Computer Engineering Department of Bioengineering Northeastern University BostonMA02115 United States The Departments of Chemical Engineering and Mathematics Northeastern University The Laboratory of Systems Pharmacology Harvard Medical School BostonMA02115 United States

We introduce the notion of non-oscillation, propose a constructive method for its robust verification, and study its application to biological interaction networks (also known as, chemical reaction networks). We begin by revisiting Muldowney's result on the non-existence of periodic solutions based on the study of the variational system of the second additive compound of the Jacobian of a nonlinear system. We show that exponential stability of the latter rules out limit cycles, quasi-periodic solutions, and broad classes of oscillatory behavior. We focus then on nonlinear equations arising in biological interaction networks with general kinetics, and we show that the dynamics of the aforementioned variational system can be embedded in a linear differential inclusion. We then propose algorithms for constructing piecewise linear Lyapunov functions to certify global robust non-oscillatory behavior. Finally, we apply our techniques to study several regulated enzymatic cycles where available methods are not able to provide any information about their qualitative global behavior. Copyright © 2020, The Authors. All rights reserved.

关键词： Piecewise linear techniques

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Plasmonic bacteria on a nanoporous mirror via hydrodynamic trapping for rapid identification of waterborne pathogens

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Light(Science & Applications) 2018年第1期7卷 359-367页

作者： Keumrai Whang Jong-Hwan Lee Yonghee Shin Wooju Lee Young Wan Kim Dongchoul Kim Luke P.Lee Taewook Kang Department of Chemical and Biomolecular Engineering Sogang UniversitySeoul 04107Korea Berkeley Sensor and Actuator Center Departments of BioengineeringElectrical Engineering and Computer ScienceBiophysics Graduate ProgramUniversity of CaliforniaBerkeleyBerkeleyCA 94720USA Department of Mechanical Engineering Sogang UniversitySeoul 04107Korea

A rapid,precise method for identifying waterborne pathogens is critically needed for effective disinfection and better ***,conventional methods,such as culture-based counting,generally suffer from slow detection times and low ***,we developed a rapid detection method for tracing waterborne pathogens by an innovative optofluidic platform,a plasmonic bacteria on a nanoporous mirror,that allows effective hydrodynamic cell trapping,enrichment of pathogens,and optical signal *** designed and simulated the integrated optofluidic platform to maximize the enrichment of the bacteria and to align bacteria on the nanopores and plasmonic mirror via hydrodynamic cell *** nanoparticles are self-assembled to form antenna arrays on the surface of bacteria,such as Escherichia coli and Pseudomonas aeruginosa,by replacing citrate with hydroxylamine hydrochloride in order to amplify the signal of the plasmonic optical *** to the synergistic contributions of focused light via the nanopore geometry,self-assembled nanoplasmonic optical antennas on the surface of bacteria,and plasmonic mirror,we obtain a sensitivity of detecting *** as low as 102 cells/ml via surface-enhanced Raman *** believe that our label-free strategy via an integrated optofluidic platform will pave the way for the rapid,precise identification of various pathogens.

关键词： mirror trapping precise

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Understanding Continuous and Pleasant Linear Sensations on the Forearm from a Sequential Discrete Lateral Skin-Slip Haptic Device

arXiv

引用

arXiv 2019年

作者： Nunez, Cara M. Williams, Sophia R. Okamura, Allison M. Culbertson, Heather Departments of Bioengineering Electrical Engineering and Mechanical Engineering Stanford University StanfordCA94305 United States Department of Computer Science University of Southern California Los AngelesCA90089 United States

A continuous stroking sensation on the skin can convey messages or emotion cues. We seek to induce this sensation using a combination of illusory motion and lateral stroking via a haptic device. Our system provides discrete lateral skin-slip on the forearm with rotating tactors, which independently provide lateral skin-slip in a timed sequence. We vary the sensation by changing the angular velocity and delay between adjacent tactors, such that the apparent speed of the perceived stroke ranges from 2.5 to 48.2 cm/s. We investigated which actuation parameters create the most pleasant and continuous sensations through a user study with 16 participants. On average, the sensations were rated by participants as both continuous and pleasant. The most continuous and pleasant sensations were created by apparent speeds of 7.7 and 5.1 cm/s, respectively. We also investigated the effect of spacing between contact points on the pleasantness and continuity of the stroking sensation, and found that the users experience a pleasant and continuous linear sensation even when the space between contact points is relatively large (40 mm). Understanding how sequential discrete lateral skin-slip creates continuous linear sensations can influence the design and control of future wearable haptic devices. Copyright © 2019, The Authors. All rights reserved.

关键词： Feedback

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