In embroidery CAD, embroidery module is one of the parts which are badly affected by requirements changes. Factory pattern provides a suitable solution to decouple client from server. In traditional factory pattern, s...
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We present JAWS, a job-aware, data-driven batch scheduler that improves query throughput for data-intensive scientific database clusters. As datasets reach petabyte-scale, workloads that scan through vast amounts of d...
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In embroidery CAD, embroidery module is one of the parts which are badly affected by requirements changes. Factory pattern provides a suitable solution to decouple client from server. In traditional factory pattern, s...
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The Wired Equivalent Privacy (WEP) protocol protection technique suggested for ad hoc network falls short of the objective of data privacy, data integrity and authentication. Various security standards such as IEEE 80...
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The Web Services are interdependent among themselves in distributed environment. Offering such interdependency is a challenging task due to geographical distance of nodes in an distributed environment which results in...
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A Top-k aggregate query, which is a powerful technique when dealing with large quantity of data, ranks groups of tuples by their aggregate values and returns k groups with the highest aggregate values. However, compar...
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We have demonstrated a heterogeneously integrated Si/III-V laser based on an ultra-large-angle super-compact curved diffraction grating suitable for WDM applications in EPICs The lasing threshold is 150mA giving a max...
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We report the design and experimental results of an electrically pumped Silicon/AlGaInAs evanescent laser with right-angled-wedge reflector defined in the silicon layer. A continuous-wave laser with a lasing threshold...
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This paper reports a novel method to chemically modify specific nucleotides in a stretched double-stranded DNA, and to label the modified nucleotides with fluoresceinated antibodies in order to visualize them using bo...
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ISBN:
(纸本)9781618390622
This paper reports a novel method to chemically modify specific nucleotides in a stretched double-stranded DNA, and to label the modified nucleotides with fluoresceinated antibodies in order to visualize them using both fluorescence microscope and AFM. We show that the modification is possible even with 48.5 kb DNA stretched to the full length and immobilized on a solid surface, where the bases are forming closed pairs and the interaction with the surface can cause steric hindrance to chemical reactions. This method is expected to be used not only for a high-resolution microscope-based direct DNA sequencing, but also for a method to align molecules and nano-particles using DNA as a template.
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