The 3D tissue construction is one of the important issues for regenerative medicine. Recently, several studies have been reported, related to realize small size of 3D tissue like cells based structure in vitro. Howeve...
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ISBN:
(纸本)9781467355599
The 3D tissue construction is one of the important issues for regenerative medicine. Recently, several studies have been reported, related to realize small size of 3D tissue like cells based structure in vitro. However, large size functional 3D tissue has not been achieved due to the size causing difficulties. In large size cells based structure, supplying oxygen and nutrients into the center part of the structure is prevented. In this case, cells at center part will die. In this paper, we propose an automated fine lattice shape structure construction system based on hydrogel fiber to produce active 3D tissues like structure. Several preliminary experiments were done to generate the suitable hydrogel fibers for our system. With respect to the experimental results, to evaluate the proposed system, the fine lattice structure was constructed as an example of 3D tissue form. Through the experiments of fine lattice shape structure construction, we observed the cell growth in the constructed lattice form, which proves the functionality of the structure. Furthermore, we designed the gel fiber discharge nozzle using laminar flow. Then, we confirmed that it is possible to form a thinner fiber in our system.
In case of emergency, we need to grasp the situation and make correct assessment quickly. The video data taken from monitoring cameras are important information in the emergent situation. In our research, we built a s...
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In case of emergency, we need to grasp the situation and make correct assessment quickly. The video data taken from monitoring cameras are important information in the emergent situation. In our research, we built a support system for identifying video data of monitoring cameras. The system collects video data from the net-cameras through the Internet and deals with them as spatiotemporal data. To search video data, we proposed an intuitive search method for users. In the method, we assume a virtual wall in the city. This method allows users to search for the video data, which is recorded during a period, and records a virtual wall in a certain direction. The users set the above search-key on the map, and it will be transformed to the search-key for the spatiotemporal access structure to proceed the search. When the system shows the search result, the video data, which is easy to understand the whole scene, will be displayed first. As the horizontal direction of the screen corresponds to the virtual wall's direction in the real world, the user can narrow down the search result in the horizontal direction of the screen. We built a prototype in Java, Javaservlet and C++.
The single cell analysis has attracted much attention to reveal the detailed and localized biological information, which is not obtained by the conventional group cell analysis due to the statistical assay, on individ...
The single cell analysis has attracted much attention to reveal the detailed and localized biological information, which is not obtained by the conventional group cell analysis due to the statistical assay, on individual cells. To analyze the detailed and localized properties of single cells, local environmental control technique, which is the technique to change and sense the local environment around single cells, is effective. Therefore, local environmental control technology for single cell analysis is desired and being established. In particular, a system that operates with stability is important. To build the system which can achieve high speed (high time resolution) and prolonged analysis is useful for an understanding of various life processes. In this study, we try to develop a real-long-time observation and cultivation system that can changing and keeping local environment condition stably and apply this system for analyze the bacteria flagella motor.
Both static and dynamic multiscalings of fluctuations of energy flux and energy dissipation rate in the Gledzer-Ohkitani-Yamada (GOY) shell model of turbulence are numerically investigated. We compute the large deviat...
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Both static and dynamic multiscalings of fluctuations of energy flux and energy dissipation rate in the Gledzer-Ohkitani-Yamada (GOY) shell model of turbulence are numerically investigated. We compute the large deviation rate function of energy flux not only in the inertial range (IR) but also around the crossover between the inertial range and the dissipation range (DR). The rate function in IR exists to be concave, which assures the applicability of the Legendre transformation with the anomalous scaling exponents that have been investigated so far, and turns out to be independent of the Reynolds number. On the contrary, near the crossover scale, an intermediate dissipation range (IMDR) scaling is observed with the rate function in IMDR, which is accounted with the argument on dissipation scale fluctuation dominated by the energy flux fluctuation in the inertial range. Furthermore, to study the difference between IR intermittency and DR intermittency, we compute finite time-averaged quantities of energy flux and energy dissipation rate and investigate their multiscaling behavior. The difference observed in terms of their dynamic multiscaling is discussed.
Problem solving abilities and the development of student interest in learning such activities is important in education, and group discussions are being introduced to classes as a means of achieving such effects. Vari...
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Problem solving abilities and the development of student interest in learning such activities is important in education, and group discussions are being introduced to classes as a means of achieving such effects. Various computerized group discussion support systems have been developed to support these classes. However, simply supporting the discussion in order to enhance student's ability to meaningfully participate in group discussions is no sufficient. The present paper focuses on the "evaluation" of the discussion so that the teacher can offer appropriate feedback to the students, which is also an important approach for improving skills necessary in discussions.
Recently, the importance of the joint design of identification and control has been recognized, and several controller design methods based on the iteration of identification and controller re-design have been propose...
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Recently, the importance of the joint design of identification and control has been recognized, and several controller design methods based on the iteration of identification and controller re-design have been proposed. In these methods, the frequency weighted identification plays an important role. On the other hand, as a powerful identification method, subspace state-space system identification (4SID) method has been proposed. However, it is difficult to use the frequency weight in conventional 4SID methods. In this paper, we propose a new frequency weighted subspace state-space system identification method for the joint design of identification and control. First, we give a new 4SID method using not only the input-output data of the system but also the nominal model data. Second, we show how to introduce the frequency weight to our identification method, which is relevant to the cost function for control. And third, we evaluate the effectiveness of the proposed method by a numerical example using an inverted pendulum system.
Prediction and observation of human motion are essential functions for robots co-existing with humans in everyday environments. We propose a people motion tracking and prediction approach by using the advantage of det...
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ISBN:
(纸本)9781467312752
Prediction and observation of human motion are essential functions for robots co-existing with humans in everyday environments. We propose a people motion tracking and prediction approach by using the advantage of detailed 3D information about the positions of body joints. Using the shoulder position displayed in a geometrical skeleton diagram of a human's upper body part, the body pose from the proposed human kinematic model is estimated, Human motion tracking and path prediction are achieved via the extended Kalman Filter. The proposed method is verified in an indoor environment where humans pass by each other. Experiment results demonstrate that walking people and their body pose are robustly tracked and predicted accurately, with less occlusions compared to traditional human tracking.
In this research, we explore using pointing and drawing in a remote collaboration system. Our application allows a local user with a tablet to communicate with a remote expert on a desktop computer. We compared perfor...
In this research, we explore using pointing and drawing in a remote collaboration system. Our application allows a local user with a tablet to communicate with a remote expert on a desktop computer. We compared performance in four conditions: (1) Pointers on Still Image, (2) Pointers on Live Video, (3) Annotation on Still Image, and (4) Annotation on Live Video. We found that using drawing annotations would require fewer inputs on an expert side, and would require less cognitive load on the local worker side. In a follow-on study we compared the conditions (2) and (4) using a more complicated task. We found that pointing input requires good verbal communication to be effective and that drawing annotations need to be erased after completing each step of a task.
Chaotic fluctuations of the order parameter in a coupled two-dimensional phase map model are numerically investigated. We discuss the system-size N dependence of the statistical properties of rare fluctuations observe...
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Chaotic fluctuations of the order parameter in a coupled two-dimensional phase map model are numerically investigated. We discuss the system-size N dependence of the statistical properties of rare fluctuations observed in the transition range between the quasiordered chaotic state and the fully developed one. It is found that the normalized probability distribution function (PDF) has a unique functional form irrespective of N. The asymptotic form of the PDF is discussed in connection with the universal distribution for correlated systems proposed by Bramwell et al. [Nature (London) 396, 552 (1998)]. Moreover, it is observed that the power spectrum PN(ω) of rare fluctuations asymptotically takes the power-law form PN(ω)∼ω−(1+α) (α=0.6∼0.7) irrespective of N. This result suggests that the temporal correlation decays as a stretched exponential.
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