To demonstrate the feasibility of using bioaugmentation to enhance biodegradation of quinoline, four strains capable of using quinoline as sole source of carbon, nitrogen and energy were isolated from different enviro...
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To demonstrate the feasibility of using bioaugmentation to enhance biodegradation of quinoline, four strains capable of using quinoline as sole source of carbon, nitrogen and energy were isolated from different environmental samples by enrichment technique. Screening for quinoline degrader with the highest quinoline mineralizing rate was carried out in respirometer and one bacterium identified as \%Burkholderia pickettii W2\% was chosen as inoculum in bioaugmentation tests. Quinoline biodegradation experiment results showed that this bacterium degraded quinoline very quickly. 100, 200 and 500 mg/L quinoline can be transformed completely within 1, 2 and 7 hours respectively. A bioaugmentation procedure was proposed and laboratory experiments confirmed that bioaugmentation was an effective way to improve the performance of traditional wastewater treatment facilities for quinoline removal. The effect of inoculum size on bioaugmentation was also investigated in this paper.
In the present research, a submerged membrane bioreactor was tested to treat domestic wastewater. Three experimental runs were conducted all with a hydraulic retention time of 5h and sludge retention times (SRTs) of 5...
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In the present research, a submerged membrane bioreactor was tested to treat domestic wastewater. Three experimental runs were conducted all with a hydraulic retention time of 5h and sludge retention times (SRTs) of 5, 10, and 20 d. The pollutant removal performance of the membrane bioreactor, the membrane effluent quality, and a kinetic model for sludge growth in the bioreactor were investigated. The combined process was capable of removing over 90% of both COD (chemical oxygen demand) and NH 3 N on the average. The total removal for COD was almost independent of SRT, but that for NH 3 N improved with increasing SRT. Membrane effluent quality meets the water quality standard for reuse issued by the Ministry of Construction of China. Increasing SRT causes the concentrations of suspended solids (SS) and volatile suspended solids (VSS) in the bioreactor to increase. However, the ratio of VSS/SS did not change much. Kinetic analysis showed that the sludge yield coefficient (kg VSS·kg COD -1 ) and the endogenous coefficient of microorganisms were 0.25 and 0.04d -1 , which are similar to those of the conventional activated sludge process.
A long term domestic wastewater treatment experiment was conducted using a recirculating ceramic ultra filtration membrane bioreactor (CUFMB) system. Three experiments were run with a hydraulic retention time of 5h, ...
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A long term domestic wastewater treatment experiment was conducted using a recirculating ceramic ultra filtration membrane bioreactor (CUFMB) system. Three experiments were run with a hydraulic retention time of 5h, sludge retention times of 5d, 15d, and 30d and a membrane surface flow rate of 4m/s. The experiment studied the membrane fouling mechanism and cleaning techniques. The results show that a CUFMB system can provide continuous good quality effluent which is completely acceptable for reuse. The system is also not affected by fluctuations of the inlet flow. The CUFMB sludge loading rate is similar to that of conventional biological treatment units. However, the volumetric loading rate of the CUFMB is 24 times that of conventional biological treatment units. Membrane fouling occurs due to channel clogging, which could be easily removed, and surface fouling, which can be effectively removed using the method described in this work which includes water rinsing, base cleaning, and acid washing.
The adsorption behaviour of 4 chlorophenol from aqueous solution to activated sludge was quantitatively characterized in this paper. The effects of the initial pH values, initial chlorophenol concentration and adsorb...
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The adsorption behaviour of 4 chlorophenol from aqueous solution to activated sludge was quantitatively characterized in this paper. The effects of the initial pH values, initial chlorophenol concentration and adsorbent dosage on bioadsorption were investigated. The maximum adsorption capacity was found to be 110.5 mg/g at 100 mg/L initial concentration. The Freundlich and Langmuir adsorption isotherms were applied to describe the biosorption processes and the isotherm constants were evaluated.
In this paper, the distribution, removal and binding forms of heavy metals in polluted seeds of rice were studied using the methods of histochemistry and biochemistry. The results indicate that the distribution of hea...
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In this paper, the distribution, removal and binding forms of heavy metals in polluted seeds of rice were studied using the methods of histochemistry and biochemistry. The results indicate that the distribution of heavy metals in rice grains was very uneven. The heavy metals in rice grains chiefly exist in association with the parts of high protein. Separation of proteins by Sephadex chromatography and subsequent analysis by atomic absorption spectroscopy revealed that Cd, Pb and As were mainly bound to protein of 54.5 and 5.50 kD molecular weights. During the rice processing, the concentrations of heavy metals in the edible rice decrease greatly, for some heavy metals in these parts of chaff, coarse rice bran and fine rice bran were removed.
With a review of advances in the high-order-accurate finite difference methods for fluid flow and heat transfer, the practical limitations of the existing methods are analyzed and the key evolution trends are noted. E...
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With a review of advances in the high-order-accurate finite difference methods for fluid flow and heat transfer, the practical limitations of the existing methods are analyzed and the key evolution trends are noted. Emphasis is put on the compact finite difference methods such as the optimal difference methods and perturbational difference methods developed recently. Some conclusions are finally given.
Microorganisms capable of degrading di-n-butyl phthalate (DBP) were immobilized by polyvinyl alcohol (PVA) and used for DBP degradation. The characteristics of DBP degradation by immobilized and free cells were invest...
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Microorganisms capable of degrading di-n-butyl phthalate (DBP) were immobilized by polyvinyl alcohol (PVA) and used for DBP degradation. The characteristics of DBP degradation by immobilized and free cells were investigated. The experimental results showed that the degradation rate of immobilized cells was higher than that of free cells. The optimal pH and temperature for DBP degradation were determined to be 7.0 and 25 degrees C, respectively. The semi-continuous degradation test demonstrated that high-frequency feeding of low concentration (50 mg/L) was more favourable than low-frequency feeding of high concentration (150 mg/L) for DBP degradation. The main intermediates of DBP degradation by immobilized cells were identified using a gas chromatography/mass spectrometry method. The results revealed the presence of monobutyl phthalate, phthalic acid, and protocatechuate. A tentative pathway for DBP degradation was proposed, which demonstrated that the metabolic mechanism of immobilized cells remained the same as that of the free cells. (C) 1997 Elsevier Science Ltd.
The biodegradation of di-n-butyl phthalate (DBP) in soil microcosoms by indigenous microbial population and a DBP degrading inoculum as well as DBP adsorption by soil was investigated. The results demonstrated that th...
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The biodegradation of di-n-butyl phthalate (DBP) in soil microcosoms by indigenous microbial population and a DBP degrading inoculum as well as DBP adsorption by soil was investigated. The results demonstrated that the adsorption of DBP by soil conformed to Freundlich equation. The indigenous bacteria were capable of degrading phthalate in soil. The innoculation of the soil with DBP degrader enhanced the DBP degradation rate. The enumeration of microorganisms indicated a good correlation between phthalate degradation and microbial counts. (C) 1997 Elsevier Science Ltd.
Activated sludge obtained from a coke-plant wastewater treatment plant was acclimated and used as seeding microbes to investigate the kinetics of biodegradation of di-n-butyl phthalate (DBP), one of the most frequentl...
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Activated sludge obtained from a coke-plant wastewater treatment plant was acclimated and used as seeding microbes to investigate the kinetics of biodegradation of di-n-butyl phthalate (DBP), one of the most frequently identified phthalic acid esters in diverse environments and the most widely used plasticizer in China. DBP biodegradation conformed to the first-order model with respect to its concentration and the rate constant was independent of the initial concentration when the initial DBP concentration was less than 200 mg/litre. (C) 1997 Elsevier Science Ltd.
A new cell immobilization method based on the replacement of KCl by KCl+chitosan as the gelling agent was developed. The experimental results showed that through addition of chitosan into gelling agent, the mechanica...
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A new cell immobilization method based on the replacement of KCl by KCl+chitosan as the gelling agent was developed. The experimental results showed that through addition of chitosan into gelling agent, the mechanical strength and the thermal stability of the carrageenan gel were greatly improved. The new immobilization method was used to entrap a chlorophenol degrading microorganism. The immobilized microbial cells were applied for chlorophenol biodegradation. The experiments demonstrated that immobilized cells exhibit a higher bioactivity in the degradation of chlorophenol than free cells.
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