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A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2

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National science Review 2022年第8期9卷 100-109页

作者： Zhi Chen Jingfeng Li Tianzhong Li Taojian Fan Changle Meng Chaozhou Li Jianlong Kang Luxiao Chai Yabin Hao Yuxuan Tang Omar A.Al-Hartomy Swelm Wageh Abdullah G.Al-Sehemi Zhiguang Luo Jiangtian Yu Yonghong Shao Defa Li Shuai Feng William J.Liu Yaqing He Xiaopeng Ma Zhongjian Xie Han Zhang Shenzhen Engineering Laboratory of Phosphorene and Optoelectronics International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education Shenzhen Institute of Translational Medicine Department of Otolaryngology Shenzhen Second People’s Hospital the First Affiliated Hospital Institute of Microscale Optoelectronics Shenzhen University Shenzhen International Institute for Biomedical Research Shenzhen Han’s Tech Limited Company Shenzhen Metasensing Tech Limited Company Department of Physics Faculty of Science King Abdulaziz University Research Center for Advanced Materials Science (RCAMS) King Khalid University Department of Chemistry College of Science King Khalid University Zhongmin (Shenzhen) Intelligent Ecology Co.Ltd Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Department of Laboratory Medicine Shenzhen Children’s Hospital Optoelectronics Research Center School of Science Minzu University of China NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Research Unit of Adaptive Evolution and Control of Emerging Viruses Chinese Academy of Medical Sciences Institute of Pathogenic Organism Shenzhen Center for Disease Control and Prevention Respiratory Department Shenzhen Children’s Hospital Institute of Pediatrics Shenzhen Children’s Hospital

The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus, and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed. On the other hand, several concerning SARS-Co V-2 variants,including Omicron, are required to be identified as soon as the samples are identified as ‘positive'.Unfortunately, a traditional PCR test does not allow their specific identification. Herein, for the first time,we have developed MOPCS(Methodologies of Photonic CRISPR Sensing), which combines an optical sensing technology-surface plasmon resonance(SPR) with the ‘gene scissors' clustered regularly interspaced short palindromic repeat(CRISPR) technique to achieve both high sensitivity and specificity when it comes to measurement of viral variants. MOPCS is a low-cost, CRISPR/Cas12a-systemempowered SPR gene-detecting platform that can analyze viral RNA, without the need for amplification,within 38 min from sample input to results output, and achieve a limit of detection of 15 fM. MOPCS achieves a highly sensitive analysis of SARS-Co V-2, and mutations appear in variants B.1.617.2(Delta),B.1.1.529(Omicron) and BA.1(a subtype of Omicron). This platform was also used to analyze some recently collected patient samples from a local outbreak in China, identified by the Centers for Disease control and Prevention. This innovative CRISPR-empowered SPR platform will further contribute to the fast, sensitive and accurate detection of target nucleic acid sequences with single-base mutations.

关键词： surface plasmon resonance CRISPR SARS-CoV-2 Omicron variant

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Small-Scale Testbeds for Connected and Automated Vehicles and Robot Swarms: Challenges and a Roadmap

arXiv

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arXiv 2025年

作者： Xu, Jianye Alrifaee, Bassam Betz, Johannes Mokhtarian, Armin Mittal, Archak Cai, Mengchi Mangharam, Rahul Shehata, Omar M. Elias, Catherine M. Zaech, Jan-Nico Scheffe, Patrick Jahncke, Felix Ulhas, Sangeet Sankaramangalam Arfvidsson, Kaj Munhoz Department of Computer Science RWTH Aachen University Germany Department of Aerospace Engineering University of the Bundeswehr Munich Germany Professorship of Autonomous Vehicle Systems Technical University of Munich Germany Department of Civil Engineering Indian Institute of Technology Bombay India School of Vehicle and Mobility Tsinghua University China Department of Electrical and Systems Engineering University of Pennsylvania United States Mechatronics Engineering Department Faculty of Engineering and Materials Science The German University in Cairo Egypt Department of Computer Science Engineering The German University in Cairo Egypt INSAIT Sofia University "St. Kliment Ohridski" Bulgaria School for Engineering of Matter Transport and Energy Arizona State University United States Division of Decision and Control Systems KTH Royal Institute of Technology Sweden

This article proposes a roadmap to address the current challenges in small-scale testbeds for Connected and Automated Vehicles (CAVs) and robot swarms. The roadmap is a joint effort of participants in the workshop "1st Workshop on Small-Scale Testbeds for Connected and Automated Vehicles and Robot Swarms," held on June 2 at the IEEE Intelligent Vehicles Symposium (IV) 2024 in Jeju, South Korea. The roadmap contains three parts: 1) enhancing accessibility and diversity, especially for underrepresented communities, 2) sharing best practices for the development and maintenance of testbeds, and 3) connecting testbeds through an abstraction layer to support collaboration. The workshop features eight invited speakers, four contributed papers [1]–[4], and a presentation of a survey paper on testbeds [5]. The survey paper provides an online comparative table of more than 25 testbeds, available at https://***/testbeds-survey. The workshop’s own website is available at https://***/iv24-workshop. Copyright © 2025, The Authors. All rights reserved.

关键词： Testbeds

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Multi-Objective Design Optimization and Sensitivity Analysis of Field Emission Electric Propulsion for Cubesat Platforms

SSRN

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SSRN 2024年

作者： Yeo, Suk Hyun Dinaol, Gadisa Ogawa, Hideaki Bang, Hyo-Choong Department of Aeronautics and Astronautics Graduate School of Engineering Kyushu University 744 Motooka Nishi-ku Fukuoka819-0395 Japan Department of Aerospace Engineering The Korea Advanced Institute of Science and Technology KAIST 291 Daejoen34141 Korea Republic of Space Systems Engineering Japan Aerospace System and Control Lab Korea Republic of

Field-emission electric propulsion is an electrostatic space electric propulsion technology that offers various advantageous features including efficient design, high specific impulse, and versatile thrust capabilities ranging from micro-Newton to milli-Newton levels. These characteristics make FEEP a promising technology for small satellite platforms, enabling precise attitude control, orbit maintenance, and de-orbiting through ionization and acceleration of a liquid metal propellant. The growing demand for small propulsion systems in CubeSat platforms has spurred significant progress in modeling and characterizing FEEP thrusters with the aim to enhance their overall performance. However, little study has been conducted to investigate the effect of geometric configurations on electric fields or expelled ion trajectories for design optimization. In this study, multi-objective design optimization is performed by incorporating electrostatic simulation coupled with an analytical performance model into evolutionary algorithms based on prediction from surrogate modeling, aiming to optimize the thruster emission design to maximize thruster performance. Physical insights into the key design factors influencing the performance of FEEP have been gained by probing into the interaction between ion particles and electric field behavior within the thruster. It has been found that the length of the emitter tip has a significant effect on plume divergence, \textit{i.e.,} a longer emitter tip under the influence of electric field at higher emitter current tends to result in lower initial acceleration of emitted ions and subsequently wider spread or divergence of the ion beam. A shorter emitter tip, on the other hand, generates a sharper E-field gradient, resulting in a more focused and narrower ion beam. Additionally, sensitivity analysis has identified the mass flow rate and potential distributions as the most influential design factors on performance due to the active roles they

关键词： Sensitivity analysis

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Upgrading and testing of the UPV/EHU Stellarator 13

Upgrading and testing of the UPV/EHU Stellarator

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13th International Conference on Information, Intelligence, systems and Applications, IISA 2022

作者： Garrido, Aitor J. Marco, Aitor De La Sen, Manuel Oleagordia, Inigo Garrido, Izaskun University of the Basque Country - UPV/EHU Automatic Control Group - Acg Institute of Research and Development of Processes - Iidp Department of Automatic Control and Systems Engineering Faculty of Engineering of Bilbao Po Rafael Moreno 3 Bilbao48013 Spain University of the Basque Country - UPV/EHU. Automatic Control Group - Ac Advanced Design and Analysis Department Idom Zarandoa Etorb. 23 Bilbao48015 Spain University of the Basque Country - UPV/EHU Automatic Control Group - Acg Institute of Research and Development of Processes - Iidp Department of Electricity and Electronics Faculty of Science and Technology B° Sarriena s/n Leioa48080 Spain

ISBN: (数字)9781665463904

ISBN: (纸本)9781665463904

This paper deals with the upgrade and testing of the of the Ultra-Low Iota Super Elongated Stellarator of the University of the Basque Country. The main upgrades affect to the coil system but also to the microwave source and the data acquisition system. Therefore, it is necessary to update its State-Space model. The updated model is validated by means of experimental output data showing an accurate matching with the real system. Then, in order to test the new model, a Model Predictive control-MPC scheme is successfully implemented both in simulation and experimentally using a real-time control platform. © 2022 IEEE.

关键词： Model predictive control

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Stabilizing control Design by Integrable Differential Passive Outputs*

Stabilizing Control Design by Integrable Differential Passiv...

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IEEE Conference on Decision and control

作者： Morteza Nazari Monfared Yu Kawano Michele Cucuzzella Department of Electrical Computer and Biomedical Engineering University of Pavia Pavia Italy Graduate School of Advance Science and Engineering Hiroshima University Higashi-hiroshima Japan Jan C. Willems Center for Systems and Control ENTEG Faculty of Science and Engineering (FSE) University of Groningen (UG) Nijenborgh 4 Groningen AG the Netherlands

ISBN: (数字)9798350316339

ISBN: (纸本)9798350316346

Differential passivity of a nonlinear system has been introduced as passivity of its variational system. Applying standard passivity-based control techniques to differentially passive systems leads to controllers for the corresponding variational systems. However, it is challenging to construct controllers for the original nonlinear systems if the differential passive outputs are non-exact differential one-forms. In this letter, our objective is to provide a systematic procedure to address this issue when differential passive outputs are integrable, i.e., when non-exact differential passive outputs can be made exact by multiplying them by suitable integrating factors. In particular, under suitable detectability assumptions, we propose one static and two dynamic state feedback stabilizing controllers, where each dynamic controller has a form of input-and output-shaping, respectively. We illustrate their effectiveness by stabilization of counter-current heat exchangers.

关键词： Heating systems State feedback Systematics control design Color Nonlinear systems Standards

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Robust flocking for non-identical second-order nonlinear multi-agent systems

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Autonomous Intelligent systems 2021年第1期1卷 81-90页

作者： Xiuxian Li Housheng Su Li Li Department of Control Science and Engineering College of Electronics and Information Engineeringand Shanghai Research Institute for Intelligent Autonomous SystemsTongji UniversityShanghai 200092China Institute for Advanced Study Tongji UniversityShanghai 200092China School of Automation Image Processing and Intelligent Control Key Laboratory of Education Ministry of ChinaHuazhong University of Science and TechnologyLuoyu Road 1037Wuhan 430074China

This paper investigates the robust flocking problem for second-order nonlinear systems with a leader and external *** contrast with most of second-order systems in the literature,the intrinsic dynamics here are nonlinear and non-identical that depend not only on the velocity but also on the position,which is more ***,the interaction topology is undirected and *** that the leader’s velocity may be constant or time-varying,two distributed flocking control laws have been proposed for two cases to make the differences of the velocities between all followers and the leader approach to zero *** proposed distributed flocking control laws are both model-independent which results in the effectiveness of the controllers to cope with the different intrinsic dynamics of the followers and the leader under some assumptions on boundedness of several *** example is given to illustrate the validity of the theoretical results.

关键词： Flocking Multi-agent systems Second-order nonlinear systems Non-identical dynamics

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Penetration Testing for IoT Security: The Case Study of a Wireless IP Security CAM 2

Penetration Testing for IoT Security: The Case Study of a Wi...

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2nd IEEE International Conference on AI in Cybersecurity, ICAIC 2023

作者： Almazrouei, Omar Magalingam, Pritheega Kamrul Hasan, Mohammad Almehrzi, Majed Alshamsi, Ahmed Universiti Teknologi Malaysia Razak Faculty of Technology and Informatics Advanced Informatics Department Kuala Lumpur Malaysia Universiti Kebngsaan Malaysia Center for Cyber Security Faculty of Information Sceince and Technology Bangi Selangor43600 Malaysia College of Technological Innovation Zayed University Abu Dhabi United Arab Emirates Masdar Institute of Science and Technology Department of Engineering Systems and Management Abu Dhabi United Arab Emirates

ISBN: (纸本)9798350396966

As the trend to use the Internet of Things (IoT) applications and devices increases, security and privacy have become key concerns. IoT application adoption has increased significantly over time, with sensitive data frequently gathered by IoT devices, accidentally or consciously. According to recent research, numerous types of IoT devices have substantial vulnerabilities, and in many cases, no security procedures are in place to secure them. The focus of this study was the security issues of Internet protocol (IP) cameras. The vulnerabilities of IP cameras were investigated in more depth, as well as their influence on security and privacy at the user level, in order to assist companies and security experts in predicting attacker behavior and securing the systems. The objective of this study was to research and uncover the security and privacy vulnerabilities associated with an IP camera. This was accomplished by performing a direct inspection of the camera. A real-world test was carried out with a VAVA Outdoor Wireless IP Security Cam, which was employed as a home security camera. Information was gathered from numerous sources on the Internet for this purpose, and then the software and hardware were used to examine the security features of this device. The findings of this study revealed that the IP camera contains security faults and vulnerabilities that put user safety at risk. © 2023 IEEE.

关键词： Internet of things

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A comprehensive review of electrochemical reduction of CO2 to methanol: Technical and design aspects

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Journal of CO2 Utilization 2023年 71卷

作者： Wiranarongkorn, Kunlanan Eamsiri, Kornkamol Chen, Yong-Song Arpornwichanop, Amornchai Center of Excellence in Process and Energy Systems Engineering Department of Chemical Engineering Faculty of Engineering Chulalongkorn University Bangkok10330 Thailand Department of Basic Science and Physical Education Faculty of Science at Sriracha Kasetsart University Chonburi20230 Thailand Department of Mechanical Engineering and Advanced Institute of Manufacturing with High-tech Innovations National Chung Cheng University Chiayi County621301 Taiwan Bio-Circular-Green-economy Technology & Engineering Center Department of Chemical Engineering Faculty of Engineering Chulalongkorn University Bangkok10330 Thailand

The electrochemical reduction of CO2 (ERC) is a promising CO2 utilization technology that can convert CO2 into a wide variety of fuels and chemicals via electrochemical reactions. Among the various products that can be produced from ERC, methanol is a potential liquid product that can be utilized as fuel and an intermediate feedstock for fuel and chemical production. Recently, many researchers have shown interest in the ERC process for selective methanol production. The development of ERC technology for methanol production has been done both in experimental studies, particularly electrocatalyst design and development, and in process design and analysis to overcome challenges such as low CO2 solubility, low product selectivity, inefficient catalysts, mass transfer limitations, high overpotentials, and technology commercialization. This review aims to present progress in studies on the production of methanol via ERC. An overview of the CO2 conversion process involving CO2 hydrogenation, ERC, and CO2 photoelectrochemical reduction is first described. Then, key factors affecting the ERC, such as the electrocatalyst, electrolyte, and operating conditions, are analyzed. Furthermore, process design and modeling analyses are discussed to consider the commercialization of the ERC to form methanol. Finally, suggestions for future research are given in the final section of this review. © 2023 The Authors

关键词： Carbon dioxide

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Enhancing Precision Agriculture Through Human-in-the-Loop Planning and control

Enhancing Precision Agriculture Through Human-in-the-Loop Pl...

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IEEE International Conference on Automation science and engineering (CASE)

作者： Shankar A. Deka Sujet Phodapol Andreu Matoses Gimenez Victor Nan Fernandez-Ayala Rufus Wong Pian Yu Xiao Tan Dimos V. Dimarogonas Department of Electrical Engineering and Automation School of Electrical Engineering Aalto University Espoo Finland Division of Decision and Control Systems School of EECS Royal Institute of Technology (KTH) Stockholm Sweden Department of Cognitive Robotics Faculty of Mechanical Engineering TU Delft Delft Netherlands Department of Computer Science University of Oxford Oxford UK

ISBN: (数字)9798350358513

ISBN: (纸本)9798350358520

In this paper, we introduce a ROS based framework designed for the planning and control of robotic systems within the context of precision agriculture, with an emphasis on human-in-the-loop capabilities. Utilizing Linear Temporal Logic to articulate complex task specifications, our algorithm creates high-level robotic plans that are not only correct by design but also adaptable in real time by human operators. This dual-focus approach ensures that while humans have the flexibility to modify the high-level plan on-the-fly or even take over low-level control of the robots, the system inherently safeguards against any human actions that could potentially breach the predefined task specifications. We demonstrate our algorithm within the dynamic and challenging environment of a real vineyard, where the collaboration between human workers and robots is critical for tasks such as harvesting and pruning, and show the practical applicability and robustness of our software. This work marks a pioneering application of formal methods to complex, real-world agricultural environments.

关键词： Precision agriculture Heuristic algorithms Software algorithms Robustness Human in the loop Software Real-time systems Planning Robots Multi-agent systems

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Survey of security, performance, and profitability of Monero: a browser-based cryptocurrency

Survey of security, performance, and profitability of Monero...

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Electrical, Computer, Communications and Mechatronics engineering (ICECCME), International Conference on

作者： Floarea Pitu Nicoleta Cristina Gaitan Faculty of Electrical Engineering and Computer Science Stefan cel Mare University of Suceava Suceava Romania Faculty of Electrical Engineering and Computer Science Stefan cel Mare University of Suceava Integrated Center for Research Development and Innovation in Advanced Materials Nanotechnologies and Distributed Systems for Fabrication and Control (MANSiD) Suceava Romania

Information is the key to success in every domain; this statement also applies to cryptocurrencies and blockchain domains. Beginning with Bitcoin back in 2009, which the anonymous Japanese Satoshi Nakamoto launched, blockchain technology emerged. This technology has revolutionized and continues to do so in digital transactions. In addition, this technology led to the further development of other cryptocurrencies that are active at these moments on the market. One class of cryptocurrencies obtained differently from classical bitcoin is those obtained due to web-based mining. The most known coins of this type are Monero, Dash, or Electroneum. This paper aims to analyze the web-based mining process of Monero and the whole process’s security. In addition, we want to explore the performance of the mining processes used in the user’s web browser. Moreover, finally, we aim to research the profitability of coins obtained due to web-browser mining, having as an object of study the cryptocurrency Monero.

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