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Application of microencapsulated essential oil extracted from Thymus schimperi for its antibacterial and antioxidant activity on minced meat

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Discover Applied Sciences 2025年第5期7卷 1-17页

作者： Asres, Yisgedu Hymete, Ariaya Admassu, Habtamu Ayalew, Amare Department of Chemical Engineering Addis Ababa Science and Technology University Addis Ababa Ethiopia Department of Food Engineering Debre Birhan University Debre Birhan Ethiopia Department of Pharmaceutical Chemistry and Pharmacognosy School of Pharmacy Addis Ababa University Addis Ababa Ethiopia Food Process Engineering Program Addis Ababa Science and Technology University Addis Ababa Ethiopia Biotechnology and Bioprocess Center of Excellence Addis Ababa Science and Technology University Addis Ababa Ethiopia Department of Chemistry Debre Birhan University Debre Birhan Ethiopia

This study investigated the antibacterial and antioxidant properties of Thymus schimperi essential oil (EO) microencapsulated using spray-drying (SD) and freeze-drying (FD) methods. The effectiveness of these encapsulated EOs was compared to sodium nitrite and an untreated meat sample, serving as a negative control, over a 15-day refrigerated storage period (4 °C). In vitro antioxidant activity, evaluated using DPPH, ABTS, and FRAP assays, revealed higher values for SD-encapsulated T. schimperi EO compared to FD-encapsulated EO. DPPH radical scavenging activity for SD-encapsulated EO ranged from 4.6 to 45.15%, while FD-encapsulated EO exhibited activity from 2.73 to 39.81%. ABTS radical scavenging activity for SD-encapsulated EO ranged from 10.45 to 35.12%, and for FD-encapsulated EO from 7.24 to 29.82%. FRAP values were 0.68 mg TE/g for SD-encapsulated EO and 0.73 mg TE/g for FD-encapsulated EO. Antibacterial activity, determined by minimum inhibitory concentration (MIC), indicated that SD-encapsulated T. schimperi EO demonstrated greater efficacy against Escherichia coli and Salmonella typhimurium (MIC: 4 mg/mL) and Staphylococcus aureus and Streptococcus epidermidis (MIC: 8 mg/mL), compared to FD-encapsulated EO (E. coli and Salmonella typhimurium: 8 mg/mL;Staphylococcus aureus: 16 mg/mL;Streptococcus epidermidis: 8 mg/mL). In situ antibacterial activity during refrigerated storage demonstrated the following order of efficacy: sodium nitrite > free T. schimperi EO > SD-encapsulated EO > FD-encapsulated EO > untreated control. These findings suggest that SD-encapsulated T. schimperi EO possesses better antibacterial and antioxidant properties compared to FD-encapsulated EO, indicating its potential as a promising natural preservative for meat products. © The Author(s) 2025.

关键词： Microencapsulation

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Enhanced production of microbial levulinic acid through deletion of the levulinic acid transcriptional regulator (lvaR) in engineered Pseudomonas putida KT2440

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Bioprocess and Biosystems engineering 2025年 1-14页

作者： Kim, Hyun Jin Kim, Byung Chan Lim, Gaeun Han, Yebin Jeong, Yunhee Kim, Hee Taek Jeon, Woo-Young Ahn, Jungoh Bhatia, Shashi Kant Yang, Yung-Hun Advanced Materials Program Department of Biological Engineering College of Engineering Konkuk University Seoul 05029 South Korea Department of Food Science and Technology Chungnam National University Daejeon South Korea Biotechnology Process Engineering Center Korea Research Institute Bioscience Biotechnology (KRIBB) Chungbuk South Korea Institute for Ubiquitous Information Technology and Application Konkuk University Seoul South Korea

Levulinic acid (LA) is a platform compound regarded as a promising organic intermediate for the synthesis of various chemicals such as fuel additives, plasticizers, solvents, and pharmaceuticals. Traditionally, LA is produced via acid-catalyzed dehydration and hydrolysis of lignocellulosic biomass, but this process involves challenges such as high temperatures and pressures, the use of strong acids, byproducts formation, and limitations in recovery and purification. To provide an alternative for chemical synthesis, we previously designed an integrated process to produce LA from glucose using genetically engineered Pseudomonas putida KT2440. However, as the consumption of the produced LA could not be completely prevented, its overall yield was limited. Therefore, in this study we constructed P. putida strains with additional knock-out of the lva operon genes (lvaAB, lvaE, and lvaR) in a pcaIJ knock-out strain, and introduced the aroG, asbF, and adc genes to design an LA production pathway. The pcaIJ, lvaR double knock-out strain P. putida HP205 produced 20.42 mM of LA from glycerol, and culture condition including temperature, glucose concentration, and nitrogen source were optimized. Under optimal conditions, P. putida HP205 produced 73.9 mM (8.58 g/L) LA in fed-batch fermentation. When crude glycerol was used as the substrate, both LA production and cell growth were enhanced. This study presents the impact of the LA transcriptional regulator and demonstrates a strategy for enhanced LA production in P. putida. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2025.

关键词： adc aroG asbF Levulinic acid lva operon lvaR

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Simultaneous extraction of crude polysaccharides, soluble proteins, gingerols, and shogaols from dried ginger by subcritical water extraction

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LWT 2025年 225卷

作者： Sakdasri, Winatta Kaomaneechot, Sunanta Lasim, Sarida Somboon, Pichayada Chotigavin, Natthaporn Wongpromrat, Patthranit Sawangkeaw, Ruengwit Program in Food Process Engineering School of Food Industry King Mongkut's Institute of Technology Ladkrabang 1 Chalong Krung 1 Alley Latkrabang Bangkok10520 Thailand Program in Fermentation Technology School of Food Industry King Mongkut's Institute of Technology Ladkrabang 1 Chalong Krung 1 Alley Latkrabang Bangkok10520 Thailand Program in Food Science and Technology School of Food Industry King Mongkut's Institute of Technology Ladkrabang 1 Chalong Krung 1 Alley Latkrabang Bangkok10520 Thailand Department of Chemical Engineering School of Engineering King Mongkut's Institute of Technology Ladkrabang 1 Chalong Krung 1 Alley Latkrabang Bangkok10520 Thailand Research Unit in Bioconversion/Bioseparation for Value-Added Chemical Production Institute of Biotechnology and Genetic Engineering Chulalongkorn University 254 Phayathai Road Pathumwan Bangkok10330 Thailand

Subcritical water extraction (SWE) is a remarkable method for the simultaneous extraction of high- and low-polarity bioactive compounds from ginger. This study investigated the effects of temperature, pressure, and extraction time on the crude polysaccharide yield, protein content, total phenolic content (TPC), and amounts of 6-gingerol and 6-shogaol. The pressure was maintained at 30 bars, and the temperature and extraction time were optimized to maximize the polysaccharide yield, protein, TPC, gingerols, and shogaols contents. The rising temperatures (100–160 °C) and extended extraction times (10–50 min) enhanced the yield and TPC but negatively affected protein stability, leading to thermal degradation when the temperature surpassed 180 °C. Specifically, the content of 6-gingerol decreased at high temperatures because of its conversion into 6-shogaol. The optimal extraction conditions were 160 °C for 40 min at 30 bars, resulting in a polysaccharide yield of 8.54 %, protein content of 100.35 mg body surface area/g dry mass, TPC of 18.10 mg Galic acid (GA)/g dry mass, 6-gingerol content of 2.14 mg/g dry mass, and 6-shogaol content of 0.28 mg/g dry mass. Therefore, SWE effectively extracts various polar bioactive compounds from plant materials in a single step. © 2025 The Authors

关键词： Ginger polysaccharides Soluble protein Gingerols Shogaol Subcritical water

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Improving the Thermo-Physical Properties of Plam Oil with Zeolite Nanoparticles

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Defect and Diffusion Forum 2024年 432卷 115-120页

作者： Assawasaengrat, Pornsawan Limsakul, Thanasuta Bunprasert, Penpichcha Chokelarb, Wasan Sriprom, Pongsert Department of Chemical Engineering School of Engineering King Mongkut's Institute of Technology Ladkrabang Bangkok Thailand Program of Food Process Engineering School of Food Industry King Mongkut's Institute of Technology Ladkrabang Bangkok Thailand

This research aimed to improve the thermo-physical properties of plam oil with NaA zeolite. Palm oil was selected for heating oil due to its high flash point and environmental friendliness compared to synthetic or mineral oil. The effect of NaA zeolite concentration suspended in palm oil was investigated in terms of the specific heat capacity (Cp), viscosity (μ), viscosity index (VI), thermal analysis (TGA) and density (ρ) of palm oil. The NaA zeolite was synthesized by crystallization technique, and X-ray diffraction analysis was performed to determine NaA zeolite crystallinity. The size of NaA zeolite was measured using a particle size analyzer. The average size of the synthesized NaA zeolite was 4767 nm. After grinding process for 180 min, the average size of zeolite decreased to 632 nm. An average 632 nm NaA zeolite particle size was added to palm oil at 0.25, 0.5, 0.75 and 1 wt%. The results showed that when the NaA zeolite concentration increased, the nanofluid’s viscosity at 40 °C and 100 °C was increased. The addition of 1 wt% NaA zeolite resulted in the nanofluid having a maximum viscosity of 41.31 and 8.47 cSt at 40 °C and 100 °C, respectively. The highest viscosity index was 195 cSt when added with 0.5 wt% NaA zeolite. The density of palm oil slightly increased with increasing NaA zeolite concentrations. The specific heat capacity of palm oil increased when NaA zeolite was added. The results showed that NaA zeolite allowed palm oil to store heat energy better than palm oil, enabling it to release and absorb more heat during heat transfer. Thermogravimetric analysis, palm oil initiated to degrade at 263 °C, while the addition NaA zeolite in palm oil indicated that palm oil initiated to degrade at 350 °C. The shift in the degradation curve demonstrated that the nanofluid could withstand high temperatures. Adding NaA zeolite to palm oil showed that palm oil could endure more heat and has a long service life. © 2024 Trans Tech Publications Ltd, Switzerland.

关键词： Palm oil

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Preparation of Activated Carbon from Durian Rind with Difference Activations and Its Optimization

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Journal of Renewable Materials 2021年第2期9卷 311-324页

作者： Pongsert Sriprom Warawut Krusong Pornsawan Assawasaengrat Program of Food Process Engineering Faculty of Agro-IndustryKing Mongkut’s Institute of Technology Ladkrabang10520BangkokThailand Program of Fermentation in Food industry Faculty of Agro-IndustryKing Mongkut’s Institute of Technology Ladkrabang10520BangkokThailand Department of Chemical Engineering Faculty of EngineeringKing Mongkut’s Institute of Technology Ladkrabang10520BangkokThailand

Durian rind wastes are an important raw material for activated carbon production due to their renewable sources and low-cost *** efficiency of increasing surface area and the quantity of oxygen groups on the surface of activated carbon were studied for the preparation of activated *** preparation of activated carbon has been studied with the different methods as follows:activation by acid,activation by base,hydrothermal and activation by acid,and hydrothermal and activation by *** results showed that hydrothermal and activation by acid had high iodine number which was chosen to determine the optimum condition for activated carbon *** optimum condition for preparation of durian rind activated carbon was studied by Box-Behnken ***/water ratio,solid/acid ratio and temperature were chosen as the important parameters for achieving the optimum reaction *** reaction products were analyzed by iodine *** on the results,the optimum condition for preparation of durian rind activated carbon was predicted using *** maximum iodine number of 626.47 mg/g was expected at the optimum condition:solid/water ratio(1:175,g/mL),solid/acid ratio(1:23,g/mL)and temperature(500℃).The preparation of durian rind activated carbon at the optimal condition was carried;the percentages of iodine number achieved(666.73±6 mg/g)were close to the maximum predicted value(666.73 mg/g),thus verifying the *** the optimum condition,the functional group on surface of durian rind activated carbon was characterized by *** result showed that the oxygen content on surface was increased in the form of carbonyl and sulfonyl group.

关键词： Durian rind activated carbon hydrothermal iodine number

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Enrichment of β-carotene in palm oil through deacidification using supercritical carbon dioxide

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Case Studies in Chemical and Environmental engineering 2025年 11卷

作者： Machmudah, Siti Wahyudiono Gautama, Pramudya Riandana Bhayu Annisa, Devi Nur Rafsanjani, Rais Rahmat Watanabe, Masaru Adschiri, Tadafumi Goto, Motonobu Food Engineering Study Program Department of Chemical Engineering Institut Teknologi Sepuluh Nopember Kampus ITS Sukolilo Surabaya 60111 Indonesia Department of Chemical Engineering Institut Teknologi Sepuluh Nopember Kampus ITS Sukolilo Surabaya 60111 Indonesia of Engineering Tohoku University 6-6-10 Aoba Aramaki Aoba-ku Sendai 980-8579 Japan New Industry Creation Hatchery Center Tohoku University 6-6-10 Aoba Aramaki Aoba-ku Sendai 980-8579 Japan WPI – Advanced Institute for Materials Research Tohoku University 6-6-10 Aoba Aramaki Aoba-ku Sendai 980-8579 Japan Department of Materials Process Engineering Nagoya University Furo-cho Chikusa-ku Nagoya 464-8063 Japan Super Critical Technology Centre Co. Ltd. Mie Kuwana 511-0838 Japan

Supercritical carbon dioxide (SCCO2) is a suitable medium for separating phytochemical compounds from various materials. Here, it was employed for crude palm oil (CPO) deacidification to concentrate the β-carotene co... 详细信息

Supercritical carbon dioxide (SCCO₂) is a suitable medium for separating phytochemical compounds from various materials. Here, it was employed for crude palm oil (CPO) deacidification to concentrate the β-carotene component. In the semibatch process, SCCO₂ was introduced to CPO feed material at various operating temperatures (40−60 °C) and pressures (15−25 MPa) with a CO₂ flow rate of 3 mL min⁻¹ for 120 min. The results indicate that the extraction yield was significantly affected by varying the operating temperature and/or pressure, and the highest extraction yield was 4.59 % (40 °C, 25 MPa). Under the same operating conditions, the addition of an ethanol entrainer improved the extraction yield to 9.31 %. However, due to the β-carotene enrichment in the raffinate product, the presence of an ethanol solvent in this process introduced challenges. © 2025 The Authors

关键词： Crude palm oil Enrichment Fractionation Supercritical CO2 β-carotene

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Optimization of Eco-Friendly Extraction and Effect of Ph on Betalain Color Properties from Dragon Fruit (Hylocereus Undatus) Peels

SSRN

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SSRN 2023年

作者： Permana, Lasuardi Sriprom, Pongsert Manamoongmongkol, Kanjana Phumjan, Lamphung Assawasaengrat, Pornsawan School of Food Industry King Mongkut’s Institute of Technology Ladkrabang Bangkok10520 Thailand Program of Food Process Engineering School of Food Industry King Mongkut’s Institute of Technology Ladkrabang Bangkok10520 Thailand Department of Chemical Engineering School of Engineering King Mongkut’s Institute of Technology Ladkrabang Bangkok10520 Thailand Study Program of Food Technology Institut Teknologi Sumatera Lampung35365 Indonesia

In this study, betalain was extracted from dragon fruit peels using a safe and environment-friendly aqueous solvent. This study investigated the effects of different pH levels, temperatures, times, and solid-to-liquid ratios on the extraction of betalain from dragon fruit peels using the Box-Behnken design as a response surface methodology. Additionally, the effect of betalain extract at pH 2.0 – pH 7.0 was examined. The results showed that the solid-to-liquid ratio had the most significant impact on betacyanin extraction as major composition of betalain in dragon fruit peels. The optimal extraction conditions were determined to be a pH of 3.6, temperature of 30°C, extraction time of 10 min, and a solid-to-liquid ratio of 1:15. The expected betacyanin content under these conditions was 72.47 mg/L. A significant change in the CIELAB parameter of the extracted betalain was observed, with marked differences in the L*, a*, and b* values as the pH increased. This color change may be attributed to the degradation of betacyanin to betalamic acid. This study demonstrates the potential of a safe and eco-friendly process for extracting betacyanin from this agricultural waste material. © 2023, The Authors. All rights reserved.

关键词： Extraction

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Insights into physical characterization of solid state fermentation: From preliminary knowledge to practical application

Journal of Biotech Research

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Journal of Biotech Research 2019年 10卷 271-282页

作者： Manan, Musaalbakri Abdul Webb, Colin Enzyme and Fermentation Technology Program Science and Food Technology Research Centre Malaysian Agricultural Research and Development Institute Persiaran MARDI - UPM Serdang Selangor43400 Malaysia Satake Centre for Grain Process Engineering School of Chemical Engineering and Analytical Science Faculty of Engineering and Physical Sciences University of Manchester United Kingdom

Solid state fermentation (SSF) involves the growth of microorganisms especially filamentous fungi on moist solid substrates in the absence of free-flowing water. SSF offers a more favorable environment for fungal growth, yielding higher productivity in a relatively low-cost process by using nutrient-rich agro-industrial residues as substrates. With the increasing interest in SSF nowadays, researchers are keen to discover as many new ways to explore the usage of this technology as possible to develop new added-value materials from by-products. In addition to nutritional composition of solid substrate used for SSF medium, physical properties of solid substrate also represent another important idea to be focused on during SSF that will influence the behavior and the productivity of microorganisms involved for substrate utilization and product formation. The main objective of this reported article was to study solid substrate particles and their physical characteristics in relation to SSF, analyzing how of these characteristics vary with different solid substrate particles and moisture contents. The importance of physical properties, such as bulk density, particle density, specific volume, porosity, particle size, surface area, volumetric specific surface area, water retention value (WRV) and tortuosity, was identified in this study. This primary information can be advantage in the microbial SSF process to be carried out. © 2019 Bio Tech System.

关键词： Microorganisms

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process intensification approach for design and optimization of biodiesel production from palm fatty acid distillate

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Biotechnology Reports 2021年 30卷 e00622页

作者： Im-orb, Karittha Arpornwichanop, Amornchai Simasatitkul, Lida Program in Food Process Engineering Faculty of Food - Industry King Mongkut's Institute of Technology Ladkrabang Bangkok 10520 Thailand Center of Excellence in Process and Energy Systems Engineering Department of Chemical Engineering Faculty of Engineering Chulalongkorn University Bangkok 10330 Thailand Department of Industrial Chemistry Faculty of Applied Science King Mongkut's University of Technology North Bangkok Bangkok 10800 Thailand

Design of the biodiesel production from palm fatty acid distillate (PFAD) using process intensification approach is studied in technical, economic and environmental view points. Firstly, the transport phenomena analysis is performed to select the suitable intensified unit. The reactive distillation is selected and used in esterification – transesterification process and hydrolysis – esterification process. The optimum condition of reactive distillation in esterification – transesterification is achieved when the methanol is fed at the 3rd stage of the 4-stage column and the liquid holdup is maintained at 6 m³. The intensified esterification – transesterification process offers higher biodiesel yield and consumes less energy compared with the intensified hydrolysis – esterification process. The economic analysis shows that the intensified esterification-transesterification process is found to be economically feasible. Finally, environment assessment based on life cycle analysis (LCA) indicates that the environmental impact of both processes are similar. © 2021 The Author(s)

关键词： Biodiesel production Economic analysis and environmental analysis Palm fatty acid distillate process design process intensification

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Enhanced Enzymatic Conversion of Durian Peel by Sulfuric Pretreatment for Biofuel Production

Enhanced Enzymatic Conversion of Durian Peel by Sulfuric Pre...

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Energy and Sustainable Development

作者： Jakaphan Ratanapoompinyo Patchanee Yasurin Theerawut Phusantisampan Prapakorn Tantayotai Elizabeth Jayex Panakkal Malinee Sriariyanun Food Biotechnology Program Faculty of Biotechnology Assumption University Bangkok Thailand Faculty of Applied Science KMUTNB Bangkok Thailand Faculty of Science Srinakharinwirot University Bangkok Thailand Chemical and Process Engineering TGGS KMUTNB Bangkok Thailand

ISBN: (数字)9781728183343

ISBN: (纸本)9781728183350

Burning of agricultural waste after harvesting seasons leads to persistent environmental pollution, especially PM2.5 and PM10. Utilization of agricultural waste by conversion to value-added product or biofuel is a solution for this problem, however, breaking down of lignocellulosic biomass in agricultural waste has a limiting factor due to its inappropriate physical and chemical properties. In this work, durian peel, as lignocellulosic biomass, was pretreated with diluted sulfuric acid to disintegrate the lignocellulosic fibrils and to promote enzymatic saccharification. To optimize this acid pretreatment, three pretreatment parameters, including temperature (60-140°C), time (20-100mins), and acid concentration (0.5-3.5%) were designed and varied based on Response Surface Methodology (RSM) using Box-Behnken design. After pretreatment, pretreated biomass was enzymatic hydrolyzed, and pretreatment efficiency was determined based on amounts of reducing sugars. The mathematical model representing the correlation of each pretreatment factor and reducing sugars was generated to calculate the optimized pretreatment condition. At predicted optimal pretreatment condition, 127.14°C, 74.13 minutes, 2.75 % , the result showed that the reducing sugar was obtained at 553.1 mg/g-durian peel, which was 1.88 folds higher compared to unpretreated durian peel. This work suggested the necessity of pretreatment in bio-conversion of agricultural waste to produce biofuels and value-added products.

关键词： Sugar industry Biomass Sugar Biofuels Temperature distribution Temperature measurement Climate change

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