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β-Fructofuranosidase and β -D-Fructosyltransferase from New Aspergillus carbonarius PC-4 Strain Isolated from Canned Peach Syrup: Effect of Carbon and Nitrogen Sources on Enzyme Production

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Scientific World Journal 2019年第1期2019卷 6956202-6956202页

作者： Do Nascimento, Gustavo Carvalho Batista, Ryhára Dias Do Amaral Santos, Claudia Cristina Auler Da Silva, Ezequiel Marcelino De Paula, Fabrício Coutinho Mendes, Danylo Bezerra De Oliveira, Deyla Paula De Almeida, Alex Fernando Bioprocess Engineering and Biotechnology Federal University of Tocantins Gurupi Tocantins Brazil Graduate Program on Food Science and Technology Federal University of Tocantins Palmas Tocantins Brazil Graduate Program on Biotechnology and Biodiversity of Amazonian-Bionorte Federal University of Tocantins Tocantins Brazil Superintendência de Desenvolvimento Científico e Tecnológico Fundação de Amparo à Pesquisa do Estado do Tocantins (FAPT) Tocantins Brazil Habite Biotechnology-Based Companies Incubator Federal University of Tocantins Gurupi Tocantins Brazil

β-fructofuranosidase (invertase) and β-D-fructosyltransferase (FTase) are enzymes used in industrial processes to hydrolyze sucrose aiming to produce inverted sugar syrup or fructooligosaccharides. In this work, a black Aspergillus sp. PC-4 was selected among six filamentous fungi isolated from canned peach syrup which were initially screened for invertase production. Cultivations with pure carbon sources showed that invertase and FTase were produced from glucose and sucrose, but high levels were also obtained from raffinose and inulin. Pineapple crown was the best complex carbon source for invertase (6.71 U/mL after 3 days of cultivation) and FTase production (14.60 U/mL after 5 days of cultivation). Yeast extract and ammonium chloride nitrogen sources provided higher production of invertase (6.80 U/mL and 6.30 U/mL, respectively), whereas ammonium nitrate and soybean protein were the best nitrogen sources for FTase production (24.00 U/mL and 24.90 U/mL, respectively). Fermentation parameters for invertase using yeast extract were Y_P/S = 536.85 U/g and P_P = 1.49 U/g/h. FTase production showed values of Y_P/S = 2,627.93 U/g and P_P = 4.4 U/h using soybean protein. The screening for best culture conditions showed an increase of invertase production values by 5.10-fold after 96 h cultivation compared to initial experiments (fungi bioprospection), while FTase production increased by 14.60-fold (44.40 U/mL) after 168 h cultivation. A. carbonarius PC-4 is a new promising strain for invertase and FTase production from low cost carbon sources, whose synthesized enzymes are suitable for the production of inverted sugar, fructose syrups, and fructooligosaccharides. © 2019 Gustavo Carvalho do Nascimento et al.

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Pharmacoinformatics and cellular studies of algal peptides as novel functional molecules to modulate type-2 diabetes markers

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Clinical Nutrition ESPEN 2024年 63卷 1119-1119页

作者： Nurkolis, F. Kurniawan, R. Basrowi, R.W. Sundjaya, T. Taslim, N.A. Hardinsyah, H. Tjandrawinata, R.R. Tallei, T.E. Kim, B. Tsopmo, A. Department of Biological Sciences Faculty of Sciences and Technology State Islamic University of Sunan Kalijaga (UIN Sunan Kalijaga) Yogyakarta Editorial Board of Journal of Indonesian Specialized Nutrition Jakarta Graduate from School of Medicine Faculty of Medicine Hasanuddin University Makassar Diabetes Connection Care Eka Hospital Bumi Serpong Damai Tangerang Magister Occupational Medicine Department of Community Medicine Faculty of Medicine Universitas Indonesia Indonesia Health Collaborative Center (HCC) Jakarta Indonesia Departement of Epidemiology Faculty of Public Health Universitas Indonesia Jakarta Division of Clinical Nutrition Department of Nutrition Faculty of Medicine Hasanuddin University Makassar Division of Applied Nutrition Faculty of Human Ecology Department of Community Nutrition IPB University Bogor Biotechnology Faculty of Biotechnology Atma Jaya Catholic University of Indonesia Jakarta Biology Faculty of Mathematics and Natural Sciences Universitas Sam Ratulangi Manado Indonesia Pathology College of Korean Medicine Kyung Hee University Seoul Republic Of Korea Food Science and Nutrition Program Department of Chemistry Carleton University Ottawa Canada

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Cover Feature: Chemo-Biological Upcycling of Poly(ethylene terephthalate) to Multifunctional Coating Materials (ChemSusChem 19/2021)

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ChemSusChem 2021年第19期14卷

作者： Prof. Hee Taek Kim Mi Hee Ryu Ye Jean Jung Sooyoung Lim Hye Min Song Prof. Jeyoung Park Prof. Sung Yeon Hwang Dr. Hoe-Suk Lee Prof. Young Joo Yeon Dr. Bong Hyun Sung Prof. Uwe T. Bornscheuer Prof. Si Jae Park Prof. Jeong Chan Joo Prof. Dongyeop X. Oh Department of Food Science and Technology Chungnam National University Daejeon 34134 (Republic of Korea Research Center for Bio-based Chemicals Korea Research Institute of Chemical Technology Daejeon 34114 & Ulsan 44429 (Republic of Korea Department of Chemical Engineering and Materials Science Graduate Program in System Health Science & Engineering Ewha Womans University Seoul 03760 (Republic of Korea Advanced Materials and Chemical Engineering University of Science and Technology (UST) Daejeon 34113 (Republic of Korea Department of Biochemical Engineering Gangneung-Wonju National University Gangneung-si Gangwon-do 25457 (Republic of Korea Synthetic Biology and Bioengineering Research Center Korea Research Institute of Bioscience and Biotechnology Daejeon 34141 (Republic of Korea Department of Biotechnology & Enzyme Catalysis Institute of Biochemistry University of Greifswald 17487 Greifswald Germany Department of Biotechnology The Catholic University of Korea Bucheon-si Gyeonggi-do 14662 (Republic of Korea

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Fish and seaweed (Eucheuma cotonii) meatball for reducing constipation effect

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AIP Conference Proceedings 2019年第1期2155卷

作者： I. Made Sugitha I. Putu Suparthana Pinky Natalia Samanta 1Food Science and Technology Study Program Faculty of Agricultural Technology Udayana University Bukit Jimbaran Badung Bali Indonesia 2Post-graduate Program Udayana University Bukit Jimbaran Badung Bali Indonesia

Functions of dietary fiber include increasing stool mass and reducing intestinal transit time which can be used to prevent constipation. This study aims to determine the ability of mixed catfish meatballs and sea grass flour to reduce constipation through two stages. The first stage was to get the best meatball quality (ratio of tapioca composition and seaweed flour manipulated at 100%: 0%; 90%: 10%; 80%: 20%; 70%: 30%) followed by the second stage which was in vivo testing for rat with doses of meatballs manipulated at 2 g, 4 g, 6 g and 8 g. Research data were analyzed with one-way variance analysis with a confidence level of 95%. Composition of tapioca and seaweed flour at 70%: 30% was the best fish meatball formula resulting in these data: 64.90% moisture content, 2.00% ash, 5.76% fat, 13.91% protein, 13.43% carbohydrate, total food fiber 5.94%) with sensory value: appearance 8.67 (smooth, not hollow, and bright surface), flavor 7.00 (less fish flavor), taste 6.67 (less meat fish taste), texture 9 (solid, compact, springy). The in vivo test showed that administration of 2 g / 200 g weigh body of rat was able to produce rat feces back to normal condition (47.93% moisture content) with a frequency of defecation 21 times per day (no constipation any more).

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INTRODUCTION

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Bulletin of the American Meteorological Society 2024年第8期105卷 S1-S11页

作者： Boyer, T. Blunden, J. Dunn, R.J.H. NOAA/NESDIS National Centers for Environmental Information Silver Spring Maryland NOAA/NESDIS National Centers for Environmental Information Asheville North Carolina Met Office Hadley Centre Exeter United Kingdom European Centre for Medium-Range Weather Forecasts Reading United Kingdom University of Maryland College Park Maryland Scripps Institution of Oceanography University of California San Diego La Jolla California NOAA/NWS National Centers for Environmental Prediction Climate Prediction Center College Park Maryland Brandenburg University of Technology (BTU) Cottbus-Senftenberg Germany Servicio Meteorológico Nacional Buenos Aires Argentina NOAA/OAR Physical Sciences Laboratory Boulder Colorado European Centre for Medium-Range Weather Forecasts Bonn Germany Center for Geophysical Research and School of Physics University of Costa Rica San José Costa Rica Department of Meteorology and National Centre for Earth Observation University of Reading Reading United Kingdom Centro Nacional de Monitoramento e Alertas de Desastres Naturais CEMADEN São Paulo Brazil Université Grenoble Alpes Institut des Géosciences de l’Environnement IRD CNRS Grenoble INP Grenoble France Hampton University Hampton Virginia Seychelles Meteorological Authority Mahe Seychelles Section for Glaciers Ice and Snow Norwegian Water Resources and Energy Directorate Oslo Norway National Research Institute for Agriculture Food and Environment (INRAE) CARRTEL Université Savoie Mont Blanc Thonon les Bains France Graduate School of Agriculture Osaka Metropolitan University Sakai Japan Science Faculty Universidad Nacional de Colombia Bogotá Colombia University of Bremen Bremen Germany NOAA Global Monitoring Laboratory Boulder Colorado Servicio Nacional de Meteorología e Hidrología del Perú Lima Perú Centro de Investigaciones sobre Desertificación – Spanish National Research Council (CSIC-UV-GVA) Valencia Spain Hydro-Climate Extremes Lab (H-CEL) Ghent University Ghent Belgium Department o

In 2023, La Niña conditions that generally prevailed in the eastern Pacific Ocean from mid-2020 into early 2023 gave way to a strong El Niño by October. Atmospheric concentrations of Earth’s major greenhouse gases—carbon dioxide, methane, and nitrous oxide—all increased to record-high levels. The annual global average carbon dioxide concentration in the atmosphere rose to 419.3±0.1 ppm, which is 50% greater than the pre-industrial level. The growth from 2022 to 2023 was 2.8 ppm, the fourth highest in the record since the 1960s. The combined short-term effects of El Niño and the long-term effects of increasing levels of heat-trapping gases in the atmosphere contributed to new records for many essential climate variables reported here. The annual global temperature across land and oceans was the highest in records dating as far back as 1850, with the last seven months (June–December) having each been record warm. Over land, the globally averaged temperature was also record high. Dozens of countries reported record or near-record warmth for the year, including China and continental Europe as a whole (warmest on record), India and Russia (second warmest), and Canada (third warmest). Intense and widespread heatwaves were reported around the world. In Vietnam, an all-time national maximum temperature record of 44.2°C was observed at Tuong Duong on 7 May, surpassing the previous record of 43.4°C at Huong Khe on 20 April 2019. In Brazil, the air temperature reached 44.8°C in Araçuaí in Minas Gerais on 20 November, potentially a new national record and 12.8°C above normal. The effect of rising temperatures was apparent in the cryosphere, where snow cover extent by June 2023 was the smallest in the 56-year record for North America and seventh smallest for the Northern Hemisphere overall. Heatwaves contributed to the greatest average mass balance loss for Alpine glaciers around the world since the start of the record in 1970. Due to rapid volume loss beginning in 2021, St. A

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Moisture sorption isotherm and shelf life of pumpkin and arrowroot starch-based instant porridge

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AIP Conference Proceedings 2020年第1期2219卷

作者： Agus Slamet Danar Praseptiangga Rofandi Hartanto Samanhudi 1Doctoral Program of Agricultural Science Graduate School of Universitas Sebelas Maret (UNS) Jl. Ir. Sutami 36 A Kentingan Surakarta 57126 Central Java Indonesia 2Department of Food Science and Technology Faculty of Agroindustry Universitas Mercu Buana Yogyakarta Jl. Wates Km 10 Yogyakarta Indonesia 3Department of Food Science and Technology Faculty of Agriculture Universitas Sebelas Maret (UNS) Jl. Ir. Sutami 36 A KentinganSurakarta 57126 Central Java Indonesia 4Department of Agrotechnology Faculty of Agriculture Universitas Sebelas Maret (UNS) Jl. Ir. Sutami 36 A Kentingan Surakarta 57126 Central Java Indonesia

Pumpkin and arrowroot starch instant porridge is a food product that is convenient to serve and appetizing to the panelist. Pumpkin and arrowroot starch instant porridge is a dry product that contains sugar; therefore, it is sensitive to moisture change during storage. Developing further product requires investigation on the proper storage condition and product stability during shelf life. Therefore, it is important to evaluate the different patterns of moisture absorption during storage. Moisture sorption isotherm of pumpkin and arrowroot starch-based instant porridge was determined using static gravimetric method at 25°C. The sample was reserved in a room RH controlled by different types of water activity (aw) from 0.07 to 0.97 to reach a balance water content. Moisture absorption was fitted to the GAB model (Guggenheim-Anderson-de Boer). The permeability of the 0.08 mm polypropylene plastic container and the shelf life of pumpkin and arrowroot starch-based instant porridge were investigated according to treatment with an accelerated technique. The result showed that the moisture sorption isotherm of pumpkin and arrowroot starch-based instant porridge showed a sigmoid curve. The shelf life of pumpkin and arrowroot starch-based instant porridge that was packed in 0.08 mm polypropylene plastic containers at 70% RH was 178.60 days or 5.96 months.

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Root microbiota regulates tiller number in rice

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Cell 2025年第12期188卷 3152-3166.e16页

作者： Zhang, Jingying Wang, Bing Xu, Haoran Liu, Weidong Yu, Jingwei Wang, Qiuxia Yu, Hong Wei, Jin-Wei Dai, Rui Zhou, Jinghang He, Yuhang Zou, Di Yang, Jinhua Ban, Xinwei Hu, Qingliang Meng, Xiangbing Liu, Yong-Xin Wang, Binglei Hu, Bin Wang, Mingyu Xin, Peiyong Chu, Jinfang Li, Changsheng Garrido-Oter, Ruben Yu, Peng van Dijk, Aalt Dirk Jan Dong, Lemeng Bouwmeester, Harro Gao, Song Huang, Ancheng Chu, Chengcai Li, Jiayang Bai, Yang Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Peking-Tsinghua Center for Life Sciences State Key Laboratory of Gene Function and Modulation Research Peking-Tsinghua-NIBS Graduate Program School of Life Sciences Peking University Beijing 100871 China CAS-JIC Centre of Excellence for Plant and Microbial Science Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China College of Advanced Agricultural Sciences University of Chinese Academy of Sciences Beijing 100101 China Shenzhen Key Laboratory of Plant Genetic Engineering and Molecular Design Institute of Plant and Food Science School of Life Sciences Southern University of Science and Technology Shenzhen 518055 China State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer Medicine Integrated Traditional Chinese and Western Medicine Research Center Sun Yat-sen University Cancer Center Guangzhou 510060 China Yazhouwan National Laboratory Sanya 572024 China School of Life Sciences Yunnan University Kunming 650504 China Guangdong Basic Research Center of Excellence for Precise Breeding of Future Crops and Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural University Guangzhou 510642 China Key Laboratory for Enhancing Resource Use Efficiency of Crops in South China Ministry of Agriculture and Rural Affairs South China Agricultural University Guangzhou 510642 China State Key Laboratory of Chemo/Biosensing and Chemometrics College of Biology Hunan University Changsha 410082 China Department of Plant-Microbe Interactions Max Planck Institute for Plant Breeding Research Cologne 50829 Germany Cluster of Excellence on Plant Sciences (CEPLAS) Düsseldorf 40225 Germany Plant Genetics TUM School of Life Sciences Technical University of Munich Freising 85354 Germany Swammerdam Institute for Life Sciences University of Amsterdam Amsterdam 1098 XH Netherlands

Rice tillering is an important agronomic trait regulated by plant genetic and environmental factors. However, the role and mechanism of the root microbiota in modulating rice tillering have not been explored. Here, we examined the root microbiota composition and tiller numbers of 182 genome-sequenced rice varieties grown under field conditions and uncovered a significant correlation between root microbiota composition and rice tiller number. Using cultivated bacterial isolates, we demonstrated that various members of the root microbiota can regulate rice tillering in both laboratory and field conditions. Genetic, biochemical, and structural analyses revealed that cyclo(Leu-Pro), produced by the tiller-inhibiting bacterium Exiguobacterium R2567, activates the rice strigolactone (SL) signaling pathway by binding to the SL receptor OsD14, thus regulating tillering. The present work provides insight into how the root microbiota regulates key agronomic traits and offers a promising strategy for optimizing crop growth by harnessing the root microbiota in sustainable agriculture. © 2025 The Authors

关键词： cyclo(Leu-Pro) dipeptide field trial microbial cultivation rice root microbiome root microbiota strigolactone pathway tiller number

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Palm oil yield potency on different level of ripening and storage time based on fruits percentage and fresh fruit bunches

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IOP Conference Series: Earth and Environmental science 2020年第1期443卷

作者： A Ruswanto A H Ramelan D Praseptiangga I B B Partha Doctoral Program of Agricultural Science Graduate School of Universitas Sebelas Maret (UNS) Jl. Ir. Sutami 36 A Kentingan Jebres 57126 Surakarta Indonesia Department of Agricultural Product Technology Faculty of Agricultural Technology INSTIPER Agricultural University Jl. Nangka II Maguwoharjo (Ring Road Utara) Sleman 55282 Yogyakarta Indonesia Department of Physics Faculty of Mathematics and Natural Sciences Universitas Sebelas Maret (UNS) Jl. Ir. Sutami 36 A Kentingan Jebres 57126 Surakarta Indonesia Department of Food Science and Technology Faculty of Agriculture Sebelas Maret University (UNS) Jl. Ir. Sutami 36 A Kentingan Jebres 57126 Surakarta Indonesia

This study aimed at assessing the oil yield potency of palm oil, based on fruits percentage (% fruit) and percentage of fresh fruit bunches (% FFB) due to the storage at different ripeness (under-ripe, ripe, over-ripe, and loose fruit from bunches) from fresh fruit bunches (FFB) originating from plantations in Ungaran, Central Java, Indonesia. A completely randomized design (CRD) with 2 factors on the ripening and the storage levels of FFB was used. The parameters observed at the initial stage were oil contents as the basis for calculation, and the yield potencies were then analyzed on the basis of % fruits and % FFB. The data obtained were analyzed by ANOVA and continued with Duncan test α = 0.05 using SPSS version 25. Results showed that both of the ripening level of palm fruit bunches and storage time significantly (α = 0.05) affected the yield potency on the basis of % fruit or % FFB, the potential yield value was based on the percentage of fruit higher than the yield potential based on the percentage of FFB. The highest yield potential based on the percentage of fruit found on ripe FFB with a storage time of 12 hours and the lowest on under-ripe FFB with a storage time of 60 hours. While the highest potential yield based on the percentage of FFB was found on ripe FFB with a storage time of 12 hours and the lowest on under-ripe FFB with a storage time of 60 hours.

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Effects of drying time on essential oil production of kaffir lime (Citrus hystrix DC) leaves at ambient temperature

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IOP Conference Series: Materials science and Engineering 2019年第1期633卷

作者： Y W Wulandari C Anwar S Supriyadi Post Graduate School of Food Science Doctor Program Gadjah Mada University (UGM) Jl. Folra no.1 Bulaksumur Yogyakarta 552812 Central Java Indonesia Department of Agricultural Products Technology Faculty of Industrial and Food Technology Slamet Riyadi University Surakarta 57136 Central Java Indonesia Department of Chemistry Faculty of Mathematics and Natural Science Gadjah Mada University Yogyakarta Indonesia Department of Food and Agricultural Products Technology Faculty of Agricultural Technology Gadjah Mada University Jl. Flora no.1 Bulaksumur Yogyakarta 552812 Indonesia

Drying was one of the primary processes in essential oil production. Time factor in drying affects both the content and chemical compositions of essential oils. This study aims to evaluate the influence of the drying time of kaffir lime leaves on the yield at room temperature and the major volatile compounds in the essential oils by observing day-to-day treatments. As a key odorants in essential oils, the quantity of citronellal was determined by Gas Chromatography (GC) using the standard compound. Based on the daily observation results, a long-time drying gave a significant effect on the yield and the major volatile compounds. The highest yield 1.26±0.07 % (w/w) was obtained on the fourth day, while the highest content of citronellal compounds, 87.58±0.33% was achieved on the fifth day of drying,. A prolonged drying time resulted in a negative effect on not only the quantity, but also the quality of essential oils. Accordingly, this study suggests that the drying technique at room temperature best lasts for four until five days to obtain the most favorable quality and quantity of essential oils.

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The repertoire of mutational signatures in human cancer (vol 578, pg 94, 2020)

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NATURE 2023年第7948期614卷 E41-E41页

作者： Alexandrov, Ludmil B. Kim, Jaegil Haradhvala, Nicholas J. Huang, Mi Ni Ng, Alvin Wei Tian Wu, Yang Boot, Arnoud Covington, Kyle R. Gordenin, Dmitry A. Bergstrom, Erik N. Islam, S. M. Ashiqul Lopez-Bigas, Nuria Klimczak, Leszek J. McPherson, John R. Morganella, Sandro Sabarinathan, Radhakrishnan Wheeler, David A. Mustonen, Ville Getz, Gad Rozen, Steven G. Stratton, Michael R. Department of Cellular and Molecular Medicine Department of Bioengineering Moores Cancer Center University of California San Diego CA USA Wellcome Sanger Institute Wellcome Genome Campus Hinxton UK Department of Cellular and Molecular Medicine and Department of Bioengineering University of California San Diego La Jolla CA USA UC San Diego Moores Cancer Center San Diego CA USA University of California San Diego San Diego CA USA Broad Institute of MIT and Harvard Cambridge MA USA Center for Cancer Research Massachusetts General Hospital Boston MA USA Massachusetts General Hospital Boston MA USA Department of Pathology Massachusetts General Hospital Boston MA USA Harvard Medical School Boston MA USA Laboratory for Medical Science Mathematics RIKEN Center for Integrative Medical Sciences Yokohama Japan Computational Biology Program Ontario Institute for Cancer Research Toronto ON Canada Programme in Cancer & Stem Cell Biology Duke-NUS Medical School Singapore Singapore Centre for Computational Biology Duke-NUS Medical School Singapore Singapore Programme in Cancer and Stem Cell Biology Duke-NUS Medical School Singapore Singapore Programme in Cancer and Stem Cell Biology Centre for Computational Biology Duke-NUS Medical School Singapore Singapore SingHealth Duke-NUS Institute of Precision Medicine National Heart Centre Singapore Singapore Singapore Cancer Science Institute of Singapore National University of Singapore Singapore Singapore Institute of Molecular and Cell Biology Singapore Singapore Laboratory of Cancer Epigenome Division of Medical Science National Cancer Centre Singapore Singapore Singapore Duke-NUS Medical School Singapore Singapore Human Genome Sequencing Center Baylor College of Medicine Houston TX USA Dan L. Duncan Cancer Center Baylor College of Medicine Houston TX USA Department of Molecular and Human Genetics Baylor College of Medicine Houston TX USA Genome Integrity and Structural Biology Laboratory National Ins

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