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Genetic variation of somaclonal mutants from the 8th generation of Pekalongan accession rodent tuber (typhonium flagelliforme lodd.) based on RAPD-PCR analysis 2

Genetic variation of somaclonal mutants from the 8th generat...

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2nd International Conference on Eco Engineering Development 2018, ICEED 2018

作者： Purnamaningsih, Ragapadmi Sianipar, Nesti Fronika Assidqi, Khoirunnisa Spits Warnars, Harco Leslie Hendric Indonesia Center for Agricultural Biotechnology and Genetic Resources Research and Development Bogor16111 Indonesia Food Technology Department Faculty of Engineering Bina Nusantara University Jakarta11480 Indonesia Research Interest Group Food Biotechnology Bina Nusantara University Jakarta11480 Indonesia Computer Science Department BINUS Graduate Program - Doctor of Computer Science Bina Nusantara University Jakarta11480 Indonesia

Rodent tuber is a potential plant which can be developed as an anticancer drug. The rodent tuber plant has a low anticancer compound which does not have an economic value to be utilized on an industrial scale, particularly to explore as an anticancer drug. The material plant was used 30 clones of Rodent Tuber Pekalongan to increase its bioactive compounds using a combination of in vitro culture with gamma-ray irradiation. The selection process has been done until the 8th generation and obtained 14 somaclonal mutants that have higher bioactive compounds than control plant (non-mutant). This study aimed to analyze the genetic variation of 14 somaclonal mutants of rodent tuber using RAPD with 14 primers. The results were produced 513 total bands with the size of 200-5000 bp and 380 polymorphic bands between somaclonal mutants. OPB-18 primer produced the most polymorphic bands and five specific polymorphic bands of 1336 bp, 1070 bp, 901 bp, 861 bp and 728 bp in the somaclonal mutant M23 (20-4-2-1-1-1). OPD-20 primer produced seven specific polymorphic bands of 1574 bp, 1557 bp, 1501 bp, 1496 bp, 1234 bp, 1229 bp and 1086 bp in the M23 somaclonal mutant. The phylogenetic analysis showed that there were two main groups with the coefficient similarity between 0.77 and 0.83. The highest genetic variation obtained was found in the M23 somaclonal mutant with a genetic difference of 23% compared with control. There are five somaclonal mutants (M24, M22, M21, M16, M14) that have a genetic variation of up to 22% over the control. This study shows that OPB-18 and OPD-20 primers were efficient in detecting somaclonal mutant variants. © 2018 Institute of Physics Publishing. All rights reserved.

关键词： Gamma rays

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A first-principles study on the quantum tunneling of methylhydroxycarbene isomerization in various solvents 4

A first-principles study on the quantum tunneling of methylh...

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4th Padjadjaran International Physics Symposium 2019, PIPS 2019

作者： Susanti, E.D. Rusydi, F. Puspitasari, I. Fadilla, R.N. Aisyah, N.D. Ahmad, A. Research Center for Quantum Engineering Design Faculty of Science and Technology Universitas Airlangga Jl. Mulyorejo Surabaya60115 Indonesia Department of Physics Faculty of Science and Technology Universitas Airlangga Jl. Mulyorejo Surabaya60115 Indonesia Precision Sciences and Technology and Applied Physics Graduate School of Engineering Osaka University Suita565-0871 Japan Information System Study Program Faculty of Science and Technology Universitas Airlangga Jl. Mulyorejo Surabaya60115 Indonesia School of Chemical Science and Food Technology Faculty of Science and Technology Universiti Kebangsaan Malaysia Bangi Selangor43600 Malaysia

Quantum tunneling phenomenon allows a high-barrier reaction to occur even though thermal energy is not sufficient to surmount the barrier. The phenomenon has been suggested to exist in the isomerization of methylhydroxycarbene which occured at 11 K. Here we study the quantum tunneling that occured in the isomerization of methylhydroxycarbene to acetaldehyde and to vinyl alcohol based on density functional calculations. The isomerization pathways are determined under the effect of various solvents which are modeled by polarizable continuum model (PCM). We use Wentzel-Kramers-Brillouin (WKB) approximation to calculate the tunneling probability (T) and extend the usefulness of T into isomerization rate. The results show that the solvents give a significant effect on the isomerization rate of methylhydroxycarbene to acetaldehyde but an insignificant one on the isomerization rate to vinyl alcohol. © 2020 IOP Publishing Ltd. All rights reserved.

关键词： Isomerization

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Anti-inflammatory effect of Momordica grosvenori Swingle extract through suppressed LPS-induced upregulation of iNOS and COX-2 in murine macrophages

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Journal of Functional foods 2009年第2期1卷 145-152页

作者： Pan, Min-Hsiung Yang, Ji-Rui Tsai, Mei-Ling Sang, Shengmin Ho, Chi-Tang Department of Seafood Science National Kaohsiung Marine University Kaohsiung 811 Taiwan Nutrition Research Program University of North Carolina Nutrition Center Julius L. Chambers Biomedical/Biotechnology Research Institute Kannapolis NC 28081 North Carolina Research Campus 500 Laureate Way United States Department of Food Science Rutgers University New Brunswick NJ 08901-8520 United States Graduate Institute of Food Science and Technology National Taiwan University Taipei 100 Taiwan

Momordica grosvenori Swingle, a traditional medicinal herb, is known to possess anti-inflammatory anti-oxidative, anti-diabetic, and nephroprotective activities. Here, we investigated the inhibitory effects of M. grosvenori Swingle extract (MSE) on the induction of NO synthase (iNOS) and cyclooxygenase-2 (COX-2) in murine RAW 264.7 cells activated with lipopolysaccharide (LPS). Western blotting and reverse transcriptase polymerase chain reaction (RT-PCR) analyses demonstrated that MSE significantly blocked protein and mRNA expression of iNOS and COX-2 in LPS-induced macrophages. Treatment with MSE resulted in the reduction of LPS-induced nuclear translocation of nuclear factor-κB (NFκB) subunit and the dependent transcriptional activity of NFκB by blocking phosphorylation of inhibitor κB (IκB)α and p65 and subsequent degradation of IκBα. Transient transfection experiments using NFκB reporter constructs indicated that MSE inhibits the transcriptional activity of NFκB in LPS-stimulated mouse macrophages. MSE also inhibited LPS-induced activation of PI3K/Akt, extracellular signal-regulated kinase 1/2 and p38 MAPK. Taken together, these results show that MSE down regulates inflammatory iNOS and COX-2 gene expression in macrophages by inhibiting the activation of NFκB by interfering with the activation PI3K/Akt/IKK and MAPK. These results have important implication for using MSE towards the development of effective anti-inflammatory agents. Crown Copyright © 2009.

关键词： Cyclooxygenase-2 (COX-2) Inducible NO synthesis (iNOS) Lipopolysaccharide (LPS) Mitogen-activated protein (MAPK) Momordica grosvenori Swingle NFκB Phosphatidylinositol 3-kinase (PI3K) RAW 264.7 monocyte/macrophages

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Antimicrobial effectiveness of biobased film against Escherichia coli 0157:H7, Listeria monocytogenes and Salmonella typhimurium

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Advance Journal of food science and technology 2011年第4期3卷 294-302页

作者： Theinsathid, Pornpun Visessanguan, Wonnop Kingchaand, Yutthana Keeratipibul, Suwimon Graduate School Interdisciplinary Program Technopreneurship and Innovation Management Chulalongkorn University Bangkok Thailand Thailand Science Park Bangkok Thailand Department of Food Technology Faculty of Science Chulalongkorn University Bangkok Thailand

Antimicrobial packaging, an active packaging concept, can be considered challenging technology that could have a significant impact on food safety of meat and meat products. The feasibility of polylactic acid (PLA)-based film was evaluated for its application as a material for antimicrobial film. A bio-based commercial polylactic acid (PLA) product, Ecovio*, was used as an environmentally friendly polymer matrix. The PLA based film was incorporated with lactic acid or sodium lactate by extrusion film-blowing process. The antimicrobial activity of films against Escherichia coli O157:H7, Listeria monocytogenes and Salmonella entérica Serovar Typhimurium (S. Typhimurium) were evaluated. Antimicrobial film incorporated with lactic acid packaging film was found to be highly effective in inhibiting L. monocytogenes. In contrast, no inhibitory activity was observed against E. coli O157:H7, and S. Typhimurium. This is consistent with Minimum Inhibitory Concentration (MIC) studies which indicated that undissociated lactic acid was more efficient in inhibiting L. monocytogenes than enterobacteria. This preliminary study shows the potential use of bio-based film as one hurdle technology in combination with good manufacturing practices and adequate storage temperatures. The use of antimicrobial packaging may contribute to improve the safety in minimally processed foods. Further work is required to improve the mechanical properties of the material in order to meet industry requirements. © Maxwell Scientific Organization, 2011.

关键词： Lactic acid

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A functional chicken-liver hydrolysate-based supplement ameliorates alcohol liver disease via regulation of antioxidation, anti-inflammation, and antiapoptosis

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Environmental Toxicology 2024年第3期39卷 1759-1768页

作者： Wu, Yi-Hsieng Samuel Lin, Yi-Ling Kao, Yi-Feng Chen, Jr-Wei Chen, Yi-Chou Chen, Yi-Chen Department of Animal Science and Technology National Taiwan University Taipei City Taiwan Institute of Food Safety and Health Risk Assessment National Yang Ming Chiao Tung University Taipei City Taiwan Undergraduate and Graduate Programs of Nutrition Science National Taiwan Normal University Taipei City Taiwan Seafood Technology Division Fisheries Research Institute Ministry of Agriculture Keelung City Taiwan Department of Animal Industry Ministry of Agriculture Taipei City Taiwan Great Billion Biotech Co. Limited. New Taipei City Taiwan Master Program in Global Agriculture Technology and Genomic Science International College National Taiwan University Taipei City Taiwan

Tons of broiler livers are produced yearly in Taiwan but always considered waste. Our team has successfully patented and characterized a chicken-liver hydrolysate (CLH) with several biofunctions. Chronic alcohol consumption causes hepatosteatosis or even hepatitis, cirrhosis, and cancers. This study was to investigate the hepatoprotection of CLH-based supplement (GBHP01™) against chronic alcohol consumption. Results showed that GBHP01™ could reduce (p © 2023 Wiley Periodicals LLC.

关键词： Metabolism

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Halstead Metric for Quality Measurement of Various Version of Statcato 5

Halstead Metric for Quality Measurement of Various Version o...

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5th International Conference on Information technology, Computer and Electrical Engineering, ICITACEE 2018

作者： Binanto, Iwan Warnars, Harco Leslie Hendric Spits Abbas, Bahtiar Saleh Sianipar, Nesti Fronika Informatics Department Sanata Dharma University Yogyakarta55002 Indonesia Computer Science Department BINUS Graduate Program Doctor of Computer Science Bina Nusantara University Jakarta11480 Indonesia Food Technology Department Bina Nusantara University Jakarta11480 Indonesia Research Interest Group Biotechnology Bina Nusantara University Jakarta11480 Indonesia

ISBN: (纸本)9781538655276

Halstead metric is one of software quality measurement technique. Some studies call it as Primitive and/or Classic Software Metric, although some researchers find vagueness of it. This paper utilizing Halstead metric to measuring the quality of various versions of Statcato software. Measurements are performed in each class of each version of Statcato software. Python programming used to facilitate this. This paper find that in terms of Halstead metric, this software seems stable during its lifecycle. So, the quality of the newer versions of Statcato are better in terms of stability of Halstead metrics than the earlier version, although its feature is increasing. © 2018 IEEE.

关键词： High level languages

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What are the Levels of Polyphenols in Brazilian Meals? An Estimate Based on the Degree of food Processing (Nova Classification)

SSRN

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SSRN 2024年

作者： Grigol, Eduarda Camila Bau Noronha, Luana Alberti Arruda, Giovana Eduarda Ranzi, Maria Eduarda Koehnlein, Eloá Angélica Federal University of Fronteira Sul Realeza Brazil Post-Graduate Program in Food Science and Technology Federal University of Fronteira Sul Laranjeiras do Sul Brazil

Considering the importance of polyphenol intake in reducing the risk of non-communicable chronic diseases, it is essential to encourage populations around the world, especially in Brazil, to consume foods that increase the presence of these compounds in their diet. This study aimed to identify the foods with the highest total polyphenol content and classes consumed by the Brazilian population, as well as their degree of processing. The study was conducted using secondary data collected from the 2017-2018 Household Budget Survey, which included the actual per capita food consumption of the Brazilian population and the usual portion size of foods. The investigation focused on 41 food items with the highest consumption frequency. Estimates of the total polyphenol (TP) content and classes in the foods were obtained from the Phenol-Explorer database, and the degree of food processing was classified according to the NOVA classification. Based on the ranking of the main foods consumed by Brazilians with the highest total polyphenol (TP) content, coffee (169.527 mg) and beans (38.422 mg) stood out for the average per capita consumption, while corn and its preparations (187.250 mg) and oranges (72.683 mg) stood out when analyzing the portion consumed in a single occasion. Regarding the polyphenol classes, the foods with the highest amounts consumed by Brazilians predominantly contained phenolic acids. Concerning the degree of processing, based on the NOVA classification, 76% of the foods with the highest polyphenol levels belonged to the fresh and minimally processed food category, while 15% corresponded to ultra-processed foods. It is essential to encourage the consumption of traditional foods in the Brazilian diet, as these are important sources of polyphenols. It is worth emphasizing the relevance of in natura and minimally processed foods as the basis of the Brazilian diet, mainly concerning the total polyphenol contents. © 2024, The Authors. All rights reserved.

关键词： food chemistry

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Improvement of the stability of nattokinase using γ-polyglutamic acid as a coating material for microencapsulation

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LWT 2009年第1期42卷 144-149页

作者： Hsieh, Chang-Wei Lu, Wen-Chien Hsieh, Wen-Ching Huang, Yun-Peng Lai, Cheng-Hung Ko, Wen-Ching Department of Medicinal Botany and Healthcare Da-Yeh University 112 Shan-Jiau Road Da-Tsuen Chang-Hua 51591 Taiwan Department of Food Science and Biotechnology National Chung-Hsing University 250 Kuokuang Road Taichung 402 Taiwan Vedan Enterprise Corporation 65 Hsingan Road Shulu Taichung 433 Taiwan Department and Graduate Program of BioIndustry Technology Da-Yeh University 112 Shan-Jiau Road Da-Tsuen Chang-Hua 51591 Taiwan

In this study, nattokinase (NK) was microencapsulated in high-molecular-weight Na-γ-PGA, and the amount of NK added to the Na-γ-PGA was in the ratio of 1:1 and 2:1 w/w, which was designated as NK-100 and NK-200, respectively. The temperature and pH stability of microencapsulated NK were found to be higher than those of the free form. Free NK lost its overall initial activity after treatment at a temperature above 60 °C or at a pH less than 5.0 for 1 h. NK-100 and NK-200 retained 35% and 60% of their activities after exposure to an acidic condition of pH 4.0 and possessed 18% and 25% activity after treatment at 60 °C for 1 h, respectively. Furthermore, NK-100 and NK-200 exhibited more protective bioactivity than free NK did in storage stability tests. This simple and versatile approach can be potentially applied to the microencapsulation of various biomolecules for drug-delivery applications. © 2008 Swiss Society of food science and technology.

关键词： Sodium

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Impact of absence of lactose on the dynamic sensory profile of yogurt: A multiple-intake TDS approach

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LWT 2022年 162卷

作者： Souza Olegario, Lary González-Mohino, Alberto Estévez, Mario Madruga, Marta Suely Ventanas, Sonia Post-Graduate Program in Food Science and Technology Department of Food Engineering Technology Centre Federal University of Paraiba Paraíba João Pessoa58051-900 Brazil IPROCAR Research Institute TECAL Research Group Universidad de Extremadura Cáceres10003 Spain

This paper aims to evaluate the impact of lactose free yogurt version on dynamic sensory perception and consumer acceptability. A total of 46 participants evaluated classical (SNY) and lactose-free (SLF-Y) commercial version of yogurts using the multiple-intake TDS technique, providing information about the dominant sequence of six flavor and texture sensory attributes. Moreover, information regarding the overall liking of the evaluated products was obtained. During the first intake, in SLF-Y samples, sourness presented a higher dominance rate compared to SNY. A similar dominance profile was obtained during the second intake between the two yogurts. During the third intake, SNY consumption was dominated by dairy attribute whereas during SLF-Y intake, fermented and creaminess were the dominance attributes. Yogurt version and the numbers of intakes presented a significant impact on temporal perception. Moreover, similar liking scores were reported for both evaluated yogurts. The present study revealed that absence of lactose showed an impact of temporal sensory perception of yogurt and the potential modifications on sensory perception can be revealed by applying the multiple intake TDS approach. © 2022 The Authors

关键词： Sensory perception

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Extraction and Characterization of Starch Fractions of Five Phenotypes <i>Pachyrhizus tuberosus</i>(Lam.) Spreng

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food and Nutrition sciences 2014年第19期5卷 1875-1885页

作者： José Luis Ramírez Ascheri Luz Haydee Bravo Zamudio Carlos Wanderlei Piler Carvalho Arturo Melendez Arevalo Lais Martins Fontoura Rheology Laboratory Embrapa Food Technology Avenida das Américas Rio de Janeiro Brazil Agronomy and Veterinary Medicine Faculty Brazilian University Campus Universitário Darcy Ribeiro Brasilia Brazil Graduate Program in Food Science and Technology Federal Rural University of Rio de Janeiro Seropédica Brazil

Pachyrhizus tuberosus is a native plant of short life cycle found in South America riverside, which provides easy starch extraction from its tuberous roots. The aim of this study was to determine the physicochemical, rheological and functionality of the starch granules extracted from the roots of five phenotypes identified as V2, V3, V4, V6 and V7. Protein and ash content of all phenotypes were considerable high when compared to other root sources such as cassava varying from 4.35% to 7.43% and 1.58% to 2.49%, respectively, whereas lipid content was lower, between 0.29 and 0.49%. The starch granules were mostly circular and polygonal with varied sizes. The starch granules structural conformation showed cristallinity A type, normally for cereals. The maximum pasting viscosity at 95℃ ranged from 1644 cP (V7) to 2232 cP (V2). The initial temperature of pasting formation occurred at 69.4℃ for V2, 71.5℃ for V3, 87.9℃ for V4, 69.5℃ for V6 and 71.5℃ for V7. These values showed high variability within the phenotypes and generally high for roots and tubers starches. The maximum viscosity at 95℃ for V2, V3, V4, V6 and V7 were 2232, 2150, 1995, 2214 and 1644 cP, respectively. The viscosity curves showed low tendency to retrogradation. The thermal properties showed that the enthalpy of gelatinization varied from 8.91 J/g (V3) to 11.78 J/g (V2). The initial gelatinization temperature varied from 63.19℃ (V6) to 65.14℃ (V4). The swelling power at 90℃ ranged from 14.7% to 20.1% p/p and solubility from 10.3% (V2) to 27.2% (V7). It is concluded that Pachyrhizus tuberosus starch showed low retrogradation (1320 - 1560 cP) comparable to non-common native waxy starches, a feature which indicates the capability of using this natural and easy extraction starch source as gelling agent in certain manufactured food of undesirable retrogradation.

关键词： Pachyrhizus tuberosus Physicochemical Rheological Starch Properties

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