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Enhanced diabetic wound healing using platelet-derived extracellular vesicles and reduced graphene oxide in polymer-coordinated hydrogels

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Journal of Nanobiotechnology 2023年第1期21卷 1-17页

作者： Hao, Ping-Chien Burnouf, Thierry Chiang, Chih-Wei Jheng, Pei-Ru Szunerits, Sabine Yang, Jen-Chang Chuang, Er-Yuan Graduate Institute of Biomedical Materials and Tissue Engineering College of Biomedical Engineering Taipei Medical University Taipei11031 Taiwan International Ph.D. Program in Biomedical Engineering College of Biomedical Engineering Taipei Medical University Taipei11031 Taiwan Graduate Institute of Biomedical Electronics and Bioinformatics National Taiwan University Taipei10617 Taiwan Department of Orthopedics Taipei Medical University Hospital Taipei11031 Taiwan Univ. Lille CNRS Centrale Lille Univ. Polytechnique Hauts-de-France UMR 8520 IEMN LilleF- 59000 France Graduate Institute of Nanomedicine and Medical Engineering College of Biomedical Engineering Taipei Medical University Taipei110-52 Taiwan Cell Physiology and Molecular Image Research Center Taipei Medical University-Wan Fang Hospital Taipei11696 Taiwan Precision Medicine and Translational Cancer Research Center Taipei Medical University Hospital Taipei11031 Taiwan

Impaired wound healing is a significant complication of diabetes. Platelet-derived extracellular vesicles (pEVs), rich in growth factors and cytokines, show promise as a powerful biotherapy to modulate cellular proliferation, angiogenesis, immunomodulation, and inflammation. For practical home-based wound therapy, however, pEVs should be incorporated into wound bandages with careful attention to delivery strategies. In this work, a gelatin-alginate hydrogel (GelAlg) loaded with reduced graphene oxide (rGO) was fabricated, and its potential as a diabetic wound dressing was investigated. The GelAlg@rGO-pEV gel exhibited excellent mechanical stability and biocompatibility in vitro, with promising macrophage polarization and reactive oxygen species (ROS)-scavenging capability. In vitro cell migration experiments were complemented by in vivo investigations using a streptozotocin-induced diabetic rat wound model. When exposed to near-infrared light at 2 W cm− 2, the GelAlg@rGO-pEV hydrogel effectively decreased the expression of inflammatory biomarkers, regulated immune response, promoted angiogenesis, and enhanced diabetic wound healing. Interestingly, the GelAlg@rGO-pEV hydrogel also increased the expression of heat shock proteins involved in cellular protective pathways. These findings suggest that the engineered GelAlg@rGO-pEV hydrogel has the potential to serve as a wound dressing that can modulate immune responses, inflammation, angiogenesis, and follicle regeneration in diabetic wounds, potentially leading to accelerated healing of chronic wounds. © 2023, BioMed Central Ltd., part of Springer Nature.

关键词： Platelets

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Utilizing a light image array with varying light intensities in optically-induced dielectrophoresis (odep)-based microfluidic system for a culture-free screen of bacteria with different responses to antibiotics treatment 23

Utilizing a light image array with varying light intensities...

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23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

作者： Chu, Po-Yu Chen, Chih-Yu Wu, Min-Hsien Ph.D. Program in Biomedical Engineering Chang Gung University Taoyuan City Taiwan Graduate Institute of Biomedical Engineering Chang Gung University Taoyuan City Taiwan Division of Haematology/Oncology Department of Internal Medicine Chang Gung Memorial Hospital at Linkou Taoyuan City Taiwan

ISBN: (纸本)9781733419000

Antibiotics resistance is regarded as a potential threat of bacterial infection, making certain bacteria survive and grow during therapy and thus resulting in therapeutic failure. To achieve a rapid screen of antibiotic-resistant bacteria, it is urgent to develop a bacteria culture-independent screening method. Integrating with properties of optically-induced dielectrophoresis (ODEP) in cell manipulation, this paper presented a culture-free screening method for bacteria with different responses to antibiotics treatment by using a light image array with varying light intensities. The results of microbial viability assay and ODEP-based bacteria manipulation have validated the concept proposed in this study. © 2019 CBMS-0001.

关键词： Bacteria

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Structural proteomics defines a sequential priming mechanism for the progesterone receptor

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Nature Communications 2025年第1期16卷 1-20页

作者： Mann, Matthew D. Wang, Min Ferreon, Josephine C. Tsoi, Phoebe S. Suess, Michael P. Jain, Antrix Malovannaya, Anna Alvarez, Roberto Vera Pascal, Bruce D. Kumar, Raj Edwards, Dean P. Griffin, Patrick R. Skaggs Graduate School of Chemical and Biological Sciences Scripps Research La Jolla CA United States Department of Molecular Medicine The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology Jupiter FL United States Department of Molecular and Cellular Biology Baylor College of Medicine Houston TX United States Verna and Marrs McLean Department of Biochemistry and Molecular Pharmacology Baylor College of Medicine Houston TX United States Mass Spectrometry Proteomics Core Facility. Advanced Technology Cores Baylor College of Medicine Houston TX United States Omics Informatics LLC Honolulu HI United States Department of Pharmaceutical and Biomedical Sciences Touro College of Pharmacy Touro University New York NY United States

The progesterone receptor (PR) is a steroid-responsive nuclear receptor with two isoforms: PR-A and PR-B. Disruption of PR-A:PR-B signaling is associated with breast cancer through interactions with oncogenic co-regulatory proteins (CoRs). However, molecular details of isoform-specific PR-CoR interactions remain poorly understood. Using structural mass spectrometry, we investigate the sequential binding mechanism of purified full-length PR and intact CoRs, steroid receptor coactivator 3 (SRC3) and p300, as complexes on target DNA. Our findings reveal selective CoR NR-box binding by PR and unique interaction surfaces between PR and CoRs during complex assembly, providing a structural basis for CoR sequential binding on PR. Antagonist-bound PR showed persistent CoR interactions, challenging the classical model of nuclear receptor activation and repression. In this work, we offer a peptide-level perspective on the organization of the PR transcriptional complex and infer the mechanisms behind the interactions of these proteins, both in active and inactive conformations. © The Author(s) 2025.

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Synthesis of bioactive evodiamine and rutaecarpine analogues under ball milling conditions

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Organic and Biomolecular Chemistry 2024年第13期22卷 2620-2629页

作者： Hu, Hao-Chun Yu, Szu-Yin Tsai, Yi-Hong Hsieh, Pei-Wen Wang, Hui-Chun Chen, Yan-Ning Chuang, Ya-Ting Lee, Min-Yu Chang, Hsueh-Wei Wu, Yang-Chang Chang, Fang-Rong Szatmári, István Fülöp, Ferenc Graduate Institute of Natural Products College of Pharmacy Kaohsiung Medical University Kaohsiung80708 Taiwan Institute of Pharmaceutical Chemistry HUN-REN-Stereochemistry Research Group University of Szeged Szeged6720 Hungary Graduate Institute of Natural Products School of Traditional Chinese Medicine College of Medicine Chang Gung University Taoyuan333 Taiwan Institute of Pharmacognosy University of Szeged Szeged6720 Hungary Department of Pharmacy and Master Program College of Pharmacy and Health Care Tajen University Pingtung County907101 Taiwan Graduate Institute of Biomedical Sciences College of Medicine Chang Gung University Taoyuan333 Taiwan Department of General Surgery Chang Gung Memorial Hospital Chiayi613 Taiwan Department of Biomedical Science and Environmental Biology PhD Program in Life Sciences College of Life Science Kaohsiung Medical University Kaohsiung80708 Taiwan Graduate Institute of Medicine College of Medicine Kaohsiung Medical University Kaohsiung80708 Taiwan Department of Otorhinolaryngology-Head and Neck Surgery Fu Jen Catholic University Hospital Fu Jen Catholic University New Taipei City242062 Taiwan School of Medicine College of Medicine Fu Jen Catholic University New Taipei City242062 Taiwan Chinese Medicine Research and Development Center China Medical University Hospital Taichung404 Taiwan Graduate Institute of Integrated Medicine China Medical University Taichung404 Taiwan Drug Development and Value Creation Research Center Kaohsiung Medical University Kaohsiung807378 Taiwan Department of Medical Research Kaohsiung Medical University Hospital Kaohsiung Medical University Kaohsiung807378 Taiwan Department of Marine Biotechnology and Resources National Sun Yat-sen University Kaohsiung80424 Taiwan

Mechanochemical reactions achieved by processes such as milling and grinding are promising alternatives to traditional solution-based chemistry. This approach not only eliminates the need for large amounts of solvents, thereby reducing waste generation, but also finds applications in chemical and materials synthesis. The focus of this study is on the synthesis of quinazolinone derivatives by ball milling, in particular evodiamine and rutaecarpine analogues. These compounds are of interest due to their diverse bioactivities, including potential anticancer properties. The study examines the reactions carried out under ball milling conditions, emphasizing their efficiency in terms of shorter reaction times and reduced environmental impact compared to conventional methods. The ball milling reaction of evodiamine and rutaecarpine analogues resulted in yields of 63-78% and 22-61%, respectively. In addition, these compounds were tested for their cytotoxic activity, and evodiamine exhibited an IC50 of 0.75 ± 0.04 μg mL−1 against the Ca9-22 cell line. At its core, this research represents a new means to synthesise these compounds, providing a more environmentally friendly and sustainable alternative to traditional approaches. © 2024 The Royal Society of Chemistry.

关键词： Ball milling

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Retraction notice to "Aptamer/doxorubicin-conjugated nanoparticles target membranous CEMIP2 in colorectal cancer" [International Journal of Biological Macromolecules 245 (2023) 125510]

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International journal of biological macromolecules 2025年 293卷 138914页

作者： Maryam Kianpour Ching-Wen Huang Pichpisith Pierre Vejvisithsakul Jaw-Yuan Wang L Chien-Feng Meng-Shin Shiao Cheng-Tang Pan Yow-Ling Shiue Institute of Biomedical Sciences College of Medicine National Sun Yat-sen University Kaohsiung 80424 Taiwan. Division of Colorectal Surgery Department of Surgery Kaohsiung Medical University Hospital Kaohsiung Medical University Kaohsiung 80756 Taiwan Department of Surgery Faculty of Medicine College of Medicine Kaohsiung Medical University Kaohsiung 80756 Taiwan. Institute of Biomedical Sciences College of Medicine National Sun Yat-sen University Kaohsiung 80424 Taiwan Program in Translational Medicine Faculty of Medicine Ramathibodi Hospital Mahidol University Bangkok 10400 Thailand. Department of Surgery Faculty of Medicine College of Medicine Kaohsiung Medical University Kaohsiung 80756 Taiwan Pingtung Hospital Ministry of Health and Welfare Pingtung 900214 Taiwan Graduate Institute of Clinical Medicine College of Medicine Kaohsiung Medical University Kaohsiung 80756 Taiwan Graduate Institute of Medicine College of Medicine Kaohsiung Medical University Kaohsiung 80756 Taiwan Center for Cancer Research Kaohsiung Medical University Kaohsiung 80756 Taiwan. Department of Medical Research Chi Mei Medical Center Tainan 71004 Taiwan Department of Pathology Chi Mei Medical Center Tainan 71004 Taiwan National Institute of Cancer Research National Health Research Institutes Tainan 70456 Taiwan. Research Center Faculty of Medicine Ramathibodi Hospital Mahidol University Bangkok 10400 Thailand. Institute of Precision Medicine College of Medicine National Sun Yat-sen University Kaohsiung 80424 Taiwan Department of Mechanical and Electro-Mechanical Engineering National Sun Yat-sen University Kaohsiung 80424 Taiwan.

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Retraction notice to "Corrigendum to 'Aptamer/doxorubicin-conjugated nanoparticles target membranous CEMIP2 in colorectal cancer' [International Journal of Biological Macromolecules 248 (2023) 125782]"

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International journal of biological macromolecules 2025年 293卷 138913页

作者： Maryam Kianpour Ching-Wen Huang Pichpisith Pierre Vejvisithsakul Jaw-Yuan Wang Chien-Feng Li Meng-Shin Shiao Cheng-Tang Pan Yow-Ling Shiue Institute of Biomedical Sciences College of Medicine National Sun Yat-sen University Kaohsiung 80424 Taiwan. Division of Colorectal Surgery Department of Surgery Kaohsiung Medical University Hospital Kaohsiung Medical University Kaohsiung 80756 Taiwan Department of Surgery Faculty of Medicine College of Medicine Kaohsiung Medical University Kaohsiung 80756 Taiwan. Institute of Biomedical Sciences College of Medicine National Sun Yat-sen University Kaohsiung 80424 Taiwan Program in Translational Medicine Faculty of Medicine Ramathibodi Hospital Mahidol University Bangkok 10400 Thailand. Department of Surgery Faculty of Medicine College of Medicine Kaohsiung Medical University Kaohsiung 80756 Taiwan Pingtung Hospital Ministry of Health and Welfare Pingtung 900214 Taiwan Graduate Institute of Clinical Medicine College of Medicine Kaohsiung Medical University Kaohsiung 80756 Taiwan Graduate Institute of Medicine College of Medicine Medical University Kaohsiung 80756 Taiwan Center for Cancer Research Kaohsiung Medical University Kaohsiung 80756 Taiwan. Department of Medical Research Chi Mei Medical Center Tainan 71004 Taiwan Department of Pathology Chi Mei Medical Center Tainan 71004 Taiwan National Institute of Cancer Research National Health Research Institutes Tainan 70456 Taiwan. Research Center Faculty of Medicine Ramathibodi Hospital Mahidol University Bangkok 10400 Thailand. Institute of Precision Medicine College of Medicine National Sun Yat-sen University Kaohsiung 80424 Taiwan Department of Mechanical and Electro-Mechanical Engineering National Sun Yat-sen University Kaohsiung 80424 Taiwan.

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The predator odor 2,4,5-trimethylthiazoline binds and activates receptor guanylyl cyclase-G to elicit innate defensive responses

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BMC Pharmacology and Toxicology 2015年第1期16卷 1-2页

作者： Ying-Chi Chao Ruey-Bing Yang Molecular Medicine Program Taiwan International Graduate Program Taiwan Institute of Biomedical Sciences Academia Sinica Taipei Taiwan Institute of Pharmacology School of Medicine National Yang-Ming University Taipei Taiwan

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Human platelet lysate biotherapy for traumatic brain injury: preclinical assessment (vol 144, pg 142, 2021)

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BRAIN 2021年第10期144卷 E93-E93页

作者： Nebie, Ouada Carvalho, Kevin Barro, Lassina Delila, Liling Faivre, Emilie Renn, Ting-Yi Chou, Ming-Li Wu, Yu-Wen Nyam-Erdene, Ariunjargal Chou, Szu-Yi Buee, Luc Hu, Chaur-Jong Peng, Chih-Wei Devos, David Blum, David Burnouf, Thierry Graduate Institute of Biomedical Materials and Tissue Engineering College of Biomedical Engineering Taipei Medical University Taipei 11031 Taiwan University of Lille Inserm CHU Lille U1172 - LilNCog—Lille Neuroscience and Cognition Lille F-59000 France Alzheimer and Tauopathies LabEx DISTALZ LiCEND Lille F-59000 France International PhD Program in Biomedical Engineering Taipei Medical University Taipei 11031 Taiwan Graduate Institute of Medical Sciences College of Medicine Taipei Medical University Taipei 11031 Taiwan Institute of Clinical Medicine National Yang-Ming University Taipei Taiwan NeuroTMULille International Laboratory Taipei Medical University Taipei 11031 Taiwan PhD Program for Neural Regenerative Medicine College of Medical Science and Technology Taipei Medical University and National Health Research Institutes Taipei 11031 Taiwan Graduate Institute of Neural Regenerative Medicine College of Medical Science and Technology Taipei Medical University Taipei 11031 Taiwan NeuroTMULille International Laboratory Univ. Lille Lille F-59000 France Dementia Center Department of Neurology Shuang Ho Hospital Taipei Medical University New Taipei City 23561 Taiwan Neurology School of Medicine College of Medicine Taipei Medical University Taipei 11031 Taiwan School of Biomedical Engineering College of Biomedical Engineering Taipei Medical University Taipei 11031 Taiwan International PhD Program in Cell Therapy and Regeneration College of Medicine Taipei Medical University Taipei 11031 Taiwan Brain and Consciousness Research Centre Taipei Medical University Shuang Ho Hospital New Taipei City 23561 Taiwan

Traumatic brain injury (TBI) leads to major brain anatomopathological damages underlined by neuroinflammation, oxidative stress and progressive neurodegeneration, ultimately leading to motor and cognitive deterioration. The multiple pathological events resulting from TBI can be addressed not by a single therapeutic approach, but rather by a synergistic biotherapy capable of activating a complementary set of signalling pathways and providing synergistic neuroprotective, anti-inflammatory, antioxidative, and neurorestorative activities. Human platelet lysate might fulfil these requirements as it is composed of a plethora of biomolecules readily accessible as a TBI *** the present study, we tested the therapeutic potential of human platelet lysate usingin vitro and in vivomodels of TBI. We first prepared and characterized platelet lysate from clinical-grade human platelet concentrates. Platelets were pelletized, lysed by three freeze-thaw cycles, and centrifuged. The supernatant was purified by 56°C 30 min heat treatment and spun to obtain the heat-treated platelet pellet lysate that was characterized by ELISA and proteomic analyses. Two mouse models were used to investigate platelet lysate neuroprotective potential. The injury was induced by an in-house manual controlled scratching of the animals’ cortex or by controlled cortical impact injury. The platelet lysate treatment was performed by topical application of 60 µl in the lesioned area, followed by daily 60 µl intranasal administration from Day 1 to 6 *** lysate proteomics identified over 1000 proteins including growth factors, neurotrophins, and antioxidants. ELISA detected several neurotrophic and angiogenic factors at ∼1–50 ng/ml levels. We demonstrate, using two mouse models of TBI, that topical application and intranasal platelet lysate consistently improved mouse motor function in the beam and rotarod tests, mitigated cortical neuroinflammation, and oxidative stress in the injury a

关键词： human platelet lysate traumatic brain injury neuroprotection neurorestoration neurotrophins

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NMR structural analysis of DNA recognition by a novel Myb1 DNA-binding domain in the protozoan parasite Trichomonas vaginalis

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Nucleic Acids Research 2009年第7期37卷 2381-2394页

作者： Lou, Yuan-Chao Wei, Shu-Yi Rajasekaran, M. Chou, Chun-Chi Hsu, Hong-Ming Tai, Jung-Hsiang Chen, Chinpan Institute of Biomedical Sciences Academia Sinica Taipei 115 Taiwan Department of Life Science National Tsing Hua University Hsinchu 300 Taiwan Chemical Biology and Molecular Biophysics Taiwan International Graduate Program Academia Sinica Taipei 115 Taiwan Graduate Institute of Life Sciences National Defense Medical Center Taipei 114 Taiwan Department of Parasitology College of Medicine National Taiwan University Taipei 106 Taiwan

The transcription regulator, tv Myb1, is the first Myb family protein identified in Trichomonas vaginalis. Using an electrophoretic mobility shift assay, we defined the amino-acid sequence from Lys ³⁵ to Ser ¹⁴¹ (tv Myb1_35-141) as the minimal DNA-binding domain, encompassing two Myb-like DNA-binding motifs (designated as R2 and R3 motifs) and an extension of 10 residues at the C-terminus. NMR solution structures of tv Myb1_35-141 show that both the R2 and R3 motifs adopt helix-turn-helix conformations while helix 6 in the R3 motif is longer than its counterpart in vertebrate Myb proteins. The extension of helix 6 was then shown to play an important role in protein stability as well as in DNA-binding activity. The structural basis for the tv Myb1_35-141/DNA interaction was investigated using chemical shift perturbations, residual dipolar couplings, DNA specificity data and data-driven macromolecular docking by HADDOCK. Our data indicate that the orientation between R2 and R3 motifs dramatically changes upon binding to DNA so as to recognize the DNA major groove through a number of key contacts involving residues in helices 3 and 6. The tv Myb1_35-141/DNA complex model furthers our understanding of DNA recognition by Myb proteins and this approach could be applied in determining the complex structures involving proteins with multiple domains.

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PD-1 blockade-unresponsive human tumor-infiltrating CD8^(+)T cells are marked by loss of CD28 expression and rescued by IL-15

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Cellular & Molecular Immunology 2021年第2期18卷 385-397页

作者： Kyung Hwan Kim Hong Kwan Kim Hyung-Don Kim Chang Gon Kim Hoyoung Lee Ji Won Han Seong Jin Choi Seongju Jeong Minwoo Jeon Hyunglae Kim Jiae Koh Bo Mi Ku Su-Hyung Park Myung-Ju Ahn Eui-Cheol Shin Graduate School of Medical Science and Engineering Korea Advanced Institute of Science and TechnologyDaejeonRepublic of Korea Department of Thoracic and Cardiovascular Surgery Samsung Medical CenterSungkyunkwan University School of MedicineSeoulRepublic of Korea Biomedical Science and Engineering Interdisciplinary Program Korea Advanced Institute of Science and TechnologyDaejeonRepublic of Korea Research Institute for Future Medicine Samsung Medical CenterSungkyunkwan University School of MedicineSeoulRepublic of Korea Department of Radiation Oncology Yonsei Cancer CenterYonsei University College of MedicineSeoulRepublic of Korea Division of Hematology-Oncology Department of MedicineSamsung Medical CenterSungkyunkwan University School of MedicineSeoulRepublic of Korea

Blockade of programmed death-1(PD-1)reinvigorates exhausted CD8^(+)T cells,resulting in tumor regression in cancer ***,reinvigoration of exhausted CD8^(+)T cells following PD-1 blockade was shown to be CD28-dependent in mouse ***,we examined the role of CD28 in anti-PD-1 antibody-induced human T cell reinvigoration using tumor-infiltrating CD8^(+)T cells(CD8^(+)TILs)obtained from non-small-cell lung cancer ***-cell analysis demonstrated a distinct expression pattern of CD28 between mouse and human CD8^(+)***,we found that human CD28^(+)CD8^(+)but not CD28^(–)CD8^(+)TILs responded to PD-1 blockade irrespective of B7/CD28 blockade,indicating that CD28 costimulation in human CD8^(+)TILs is dispensable for PD-1 blockade-induced reinvigoration and that loss of CD28 expression serves as a marker of anti-PD-1 antibody-unresponsive CD8^(+)*** and phenotypically,PD-1 blockade-unresponsive human CD28^(–)PD-1^(+)CD8^(+)TILs exhibited characteristics of terminally exhausted CD8^(+)T cells with low TCF1 ***,CD28^(–)PD-1^(+)CD8^(+)TILs had preserved machinery to respond to IL-15,and IL-15 treatment enhanced the proliferation of CD28^(–)PD-1^(+)CD8^(+)TILs as well as CD28^(+)PD-1^(+)CD8^(+)*** together,these results show that loss of CD28 expression is a marker of PD-1 blockade-unresponsive human CD8^(+)TILs with a TCF1–signature and provide mechanistic insights into combining IL-15 with anti-PD-1 antibodies.

关键词： anti-PD-1 T cells TCF1 CD28 IL-15

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