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Determination of drugs of abuse in oral fluid using dried oral fluid spot assisted by 24-well plate and LC-MS/MS

Bioanalysis 2025年第9期17卷 595-605页

作者： Gabriela Ramos Borges Bruno Pereira dos Santos Giovanna Cristiano de Gouveia Carolina Silveira Dalanhol Juliana Nichterwitz Scherer Sarah Eller Tiago Franco de Oliveira Graduate Program in Health Sciences Federal University of Health Sciences of Porto Alegre (UFCSPA) Porto Alegre RS Brazil. Programa de Pós-Graduação em Saúde Coletiva Universidade do Vale do Rio dos Sinos (UNISINOS) São Leopoldo RS Brazil.

BACKGROUND:Oral fluid has proven to be an excellent matrix for drug testing. Several analytical techniques are available, including the use of dried spots, which have gained increasing recognition in recent years. In this study, a new method was developed and validated using dried oral fluid spot (doFS) assisted by a 24-well plate for the simultaneous determination of thirteen drugs of abuse and metabolites in oral fluid by LC - MS/MS. METHODS:Samples were collected by expectoration and diluted with water. A 100 μL aliquot of sample was applied onto the spot, dried for 60 min, and extracted with 250 μL of acetonitrile:methanol (3:1). The method was validated according to the ANSI/ASB Standard 036 guideline, and 86 oral fluid samples were analyzed. RESULTS:The limits of quantification ranged from 1 to 5 ng/mL, and all calibration curves were linear. Precisions ranged from 2.0 to 13.0% and Bias fluctuated from -19.7 to 9.6%. Ionization suppression or enhancement was noted at -16.2 to 16.6%. Among the analyzed samples, 86% tested positive for at least one substance. CONCLUSIONS:The findings confirm that the proposed method was successfully validated, providing a new bioanalytical approach for the detection of drugs of abuse in oral fluid.

关键词： LC–MS/MS Oral fluid cocaine dried oral fluid spots drug testing stimulants

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Ubiquitous computing in light of human phenotypes: foundations, challenges, and opportunities

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Journal of Ambient Intelligence and Humanized computing 2023年第3期14卷 2341-2349页

作者： Bavaresco, Rodrigo Simon Barbosa, Jorge Luis Victória Applied Computing Graduate Program - PPGCA University of Vale do Rio dos Sinos - UNISINOS Av. Unisinos São Leopoldo Rio Grande do Sul93.022-000 Brazil

The interest in human phenotypes has leveraged interdisciplinary efforts encouraging a better understanding of the broad spectrum of psychological and behavioral disorders. Moreover, the usage of mobile and wearable devices along with unobtrusive computational capabilities provides an extensive amount of information that allows the characterization of phenotypes. This article describes the human phenotype through the lens of computational range and reviews state-of-the-art computational phenotyping. Furthermore, the article discusses computational phenotyping’s extension concerning the combination of intelligent environments and personal mobile devices, addressing technical, managerial, and ethical challenges. This combination reinforces ubiquitous computational capabilities for phenotyping as a facilitator of interdisciplinary information convergence in favor of clinical and biomedical research. © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

关键词： Wearable technology

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On proposing a non-intrusive device and methodology to monitor motor degradation

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Journal of King Saud university - Engineering Sciences 2023年第3期35卷 215-223页

作者： Kuhn, Henrique Cafruni da Rosa Righi, Rodrigo Crovato, Cesar David Paredes Electrical Engineering Graduate Program Universidade do Vale do Rio dos Sinos São Leopoldo Brazil Applied Computing Graduate Program Universidade do Vale do Rio dos Sinos São Leopoldo Brazil

Monitoring the degradation of induction motors is an essential concern for industries, especially when there are high costs related to maintenance and their stops. Nowadays, there are many non-invasive ways to monitor the health status of induction motors, including sensors to measure temperature, magnetic field, vibration, current, and power. However, installing these sensors may not be a simple task since intrusive procedures such as engine disassembling, wire separation, and engine interruption are commonly observed. Thus, we propose MUCLA (Multiphase Clamp and Tracking - patent pending) as a new device and methodology for monitoring motor degradation by analyzing its stator current. MUCLA introduces a non-invasive current sensors array on a proposed clamp in the hardware perspective, while a spectral tracking methodology refers to our novelty in the software viewpoint. For end-users, the main MUCLA contribution relies on the combination of the simplicity of installation and the possibility of using the proposed device even when considering Variable-Frequency Drive powered engines. The results obtained with both simulation and practical tests show encouraging results over different input workloads and emulated degradation. Compared to the current initiatives, we can classify MUCLA as a better solution for industrial plants in terms of installation time, installation complexity, and cost. © 2021 The Authors

关键词： Induction motors

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Patients' emotional expressions and clinicians' responses in oncology – From recognition to exploration of concerns

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PEC Innovation 2025年 6卷 100374-100374页

作者： Romeiro, Fernanda Bittencourt Pais, Vanessa Garrido Humphris, Gerry Figueiredo-Braga, Margarida University of Vale do Rio dos Sinos-Unisinos Graduate Program of Psychology Brazil Medical Psychology Unit Department of Clinical Neurosciences and Mental Health School of Medicine University of Porto Porto Portugal Health Psychology School of Medicine University of St. Andrews Scotland United Kingdom Medical Psychology Unit Dep Clinical Neurosciences and Mental Health Faculty of Medicine University of Porto Portugal

Objectives: The objective of this study was to analyze patient's emotional expressions during the consultations and the responses of their oncologists to these expressions. Methods: The study employed a mixed-method, observational, descriptive, and explanatory design. A total of 31 adult patients at different clinical stages, undergoing cancer treatment and 8 oncologists were included. Thirty-one routine outpatient oncology consultations were analyzed, after being transcribed and coded, using the Portuguese version of the Verona Coding Definitions of Emotional Sequences (VR-CoDES). Results: The oncologists elicited and recognized patients' emotional concerns but they did not explore them in a way that encouraged patients to continue verbalizing their feelings. Oncologists provided more directive and guidance-oriented responses, focusing on cues related to physical pain and symptoms. Multilevel logistic regression analysis modeled the probability of oncologists' responses showing reduction of space in relation to patients' emotional cues/concerns, controlling for clustering and patients' clinical and socio-demographic variables. The type of cue and treatment influenced the oncologists' responses. Conclusions: Communication skills training focused on the ability to better explore patients' emotions may help oncologists to provide more explicit and empathetic responses that validate the emotional content expressed during consultations. Practice Implications: Oncologists do not use the same responses as a standard with patients, thus adjusting them individually. © 2025

关键词： Emotional expressions Multi-level Modelling Oncology Patient-Clinician Communication VR-CoDES

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Monitoring Heat Waves and Their Impacts on Summer Crop Development in Southern Brazil

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Agricultural Sciences 2014年第4期5卷 353-364页

作者： Anibal Gusso Jorge Ricardo Ducati Mauricio Roberto Veronez Victor Sommer Luiz Gonzaga da Silveira Junior Environmental Engineering Vale do Rio dos Sinos University(UNISINOS)Sao LeopoldoBrazil Center for Remote Sensing and Meteorological Research Federal University of Rio Grande do Sul(UFRGS)Porto AlegreBrazil VizLab-Advanced Visualization Laboratory Vale do Rio dos Sinos University(UNISINOS)Sao LeopoldoBrazil Astronomy Department Federal University of Rio Grande do Sul(UFRGS)Porto AlegreBrazil Graduate Program in Geology Vale do Rio dos Sinos University(UNISINOS)Sao LeopoldoBrazil Crop Breeding Foundation(Fundacao Pró-Sementes) Passo FundoBrazil Graduate Program in Applied Computing Vale do Rio dos Sinos University(UNISINOS)São LeopoldoBrazil

Periods in the soybean summer cycle that are sensitive to the occurrence of high temperatures were studied. An analysis was performed on the variability of soybean yields associated with crop canopy temperatures during key development periods. A land surface temperature (LST) data series from MODIS (Moderate Resolution Imaging Spectroradiometer) on the Aqua satellite was processed between 2003 and 2012 that covered the entire state of rio Grande do Sul, in Brazil. Enhanced vegetation index (EVI) data from MODIS on the Terra satellite were used to monitor the LST during different phenological stages. Spatially interpolated maps of soybean yield distributions were generated using data obtained from Instituto Brasileiro de Geografia e Estatística (IBGE) at state and municipality levels. The results indicate that canopy-LST occurrence in mid-February, during the grain filling, is most correlated to yield reduction (R2 = 0.82 and RMSD = 14.4%). At the state level, the average yield is 2003 kg·ha-1 with a standard deviation of 308 kg·ha-1. The overall average of the canopy-LST is 305.0 K (31.8°C) with a standard deviation of 1.9 K. The slope of the downward linear relationship between canopy-LST and yield was -28.7%. These results indicate that monitoring heat wave events can provide important information for characterising agriculture vulnerability.

关键词： Land Surface Temperature Soybean Remote Sensing Canopy Vegetation Index MODIS Data

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GUGA: A tool for participatory cities

GUGA: A tool for participatory cities

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18th IEEE Symposium on Computers and Communications, ISCC 2013

作者： Schunke, Luana Carine De Oliveira, Luiz Paulo Luna Villamil, Marta Becker Applied Computing Graduate Program University of Vale Do Rio Dos Sinos-Unisinos S˜ao Leopoldo-RS Brazil

ISBN: (纸本)9781479937554

Participatory cities are those that allow the participation of citizens in the construction and improvement of their common daily life. In this work we present a tool for that participation, allowing the citizens to register the occurrences as well as take knowledge about the global geographic status of their city in on a variety of contexts (security, transit, etc.). However, a tool like that, fed by the people, certainly will deal with a large amount of information, distributed geographically. To accomplish that, a precise criterion for visualizing the resultant density distribution of occurrences must be used. In this paper, we also propose an automatic method for that visualization based on Voronoi Diagrams. The Voronoi polygons divide the region according to the concentration of occurrences. In this scheme, the area of each Voronoi polygon defines the density of the occurrences of the specific categories which are shown in the city map in color and transparency intensities for visualization. This can easily reveal the space distribution of important issues, in a variety of contexts such as criminality, commercial and industrial activities, drugs traffic, illumination concerns, and others. © 2013 IEEE.

关键词： Visualization

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An ontology-based repository for a spontaneous social network

An ontology-based repository for a spontaneous social networ...

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2011 International Conference on Computational Aspects of Social Networks, CASoN'11

作者： Silva, Bryan Garber Da Costa, Cristiano André Da Zaupa, Dante Freitas, Gustavo Universidade Do Vale Do Rio Dos Sinos - UNISINOS São Leopoldo Brazil Applied Computing Graduate Program - PIPCA Universidade Do Vale Do Rio Dos Sinos - UNISINOS São Leopoldo Brazil

ISBN: (纸本)9781457711312

With the latest technological advancements and the popularization of mobile devices nowadays, software development has evolved towards mobility. One of the main uses of mobile devices is to access social networks. They are becoming important tools in people's life. In this context, the Mingle project has the objective of creating a spontaneous social networks that is mainly accessed by mobile devices, such as smartphones. Mingle uses the concepts of ubiquitous computing to create a notion of spontaneity, i.e, providing a social network adapted to the persons specific location. This article has the goal of presenting an ontology and the persistence model for managing the information inside a specific location (called "Cell" in our model). To evaluate our proposal, two technics were applied: lexical evaluation and application-level evaluation. The first assessment employs the Levenshtein edit distance and compares Mingle with other popular ontologies in the field, while the second one evaluates if we deal with all Mingle requirements. © 2011 IEEE.

关键词： Ontology

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Visualization and analysis of interacting occurences in a smart city 19

Visualization and analysis of interacting occurences in a sm...

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19th IEEE Symposium on Computers and Communications, ISCC 2014

作者： Schunke, Luana Carine De Oliveira, Luiz Paulo Luna Villamil, Marta Becker Applied Computing Graduate Program Universidade Do Vale Do Rio Dos Sinos - Unisinos São Leopoldo - RS Brazil

ISBN: (纸本)9781479942787

New information and communication technologies enable a better management of the available resources in a smart city. Leaders need tools to analyze data for better decisions, anticipate problems and resolve them proactively. In this context, this article proposes a simulation tool to analyze the interaction of typical cities occurrences like criminal and police. The information of both occurrences, including their densities, are inserted and visualized on a city map. Each record is marked on the GPS point where it happened. From these inputs data, the interactions are modeled by Voronoi Diagrams. The occurrences change their position according to the size area of the opposite event. In this way, crimes runway from police and police catch crimes occurrences. As results, we show that a large increase of police actions will not influence quantitatively in the reduction of criminal occurrences out of a critical zone. © 2014 IEEE.

关键词： Crime

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Internet of things scalability: Analyzing the bottlenecks and proposing alternatives 6

Internet of things scalability: Analyzing the bottlenecks an...

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2014 6th International Congress on Ultra Modern Telecommunications and Control Systems and Workshops, ICUMT 2014

作者： Gomes, Márcio Da Rosa Righi, Rodrigo Da Costa, Cristiano André Applied Computing Graduate Program Universidade Do Vale Do Rio Dos Sinos Brazil

ISBN: (纸本)9781479952915

Internet of Things, or IoT, is a new revolution of the Internet, supporting a connection between objects and humans yet more strong. This area is known by the huge quantity of data that can be generated from the RFID sensors at realtime. On managing this data, the IoT middleware introduces some time constrains regarding both accessing applications and filtering and storing data actions. Thus, middleware scalability is a key requirement for supporting the current growth of IoT. In this context, this article firstly presents μIB - Micro Internet of Things Benchmark - that aims at evaluating the scalability of EPC-compliant middlewares. μIB combines data from tags, RFID readers, sequential requests and number of threads to compose an evaluation setup to assess EPCIS and ALE components. We used this benchmark to evaluate Fosstrak, which is one of the most used IoT middlewares, in a single server. The tests emphasized that the exhaustion in one component influences the performance of the others directly. Following this research line, this article also describes the first ideas on redesigning the IoT middleware using techniques such as cloud virtualization and elasticity, service decomposition and multithreading programming. © 2014 IEEE.

关键词： Middleware

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A proposal of a mobile payment system based on android

A proposal of a mobile payment system based on android

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6th International Conference on Innovative Mobile and Internet Services in Ubiquitous computing, IMIS 2012

作者： Roehrs, Alex Da Costa, Cristiano André Barbosa, Jorge Luis Victória Applied Computing Graduate Program - PIPCA Universidade Do Vale Do Rio Dos Sinos - UNISINOS Sáo Leopoldo RS Brazil

ISBN: (纸本)9780769546841

This paper aims at presenting an architectural model proposal for a novel Mobile Payment System, called 4iPay. This work considers the following premises: independence of device, location, carrier and cardholder to meet the needs of executing payment transactions in ubiquitous commerce. Our proposal considers the convergence of concepts of ubiquity, unity, universality and unison to form the proposed model. This article describes the model, implementation, preliminary assessments and requirements for mobile payment in ubiquitous environments. We developed a prototype of 4iPay using Android smart phones. The prototype was evaluated in three different scenarios. © 2012 IEEE.

关键词： Android (operating system)

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