Erythritol- and mannose-containing glycolipids have been found in the intracellular lipids of a strain of the com smut fungus, Ustilago maydis. The lipids have been fractionated by Florisil column chromatography and t...
An instrument is described for transport studies in a short-circuited system which can be easily and inexpensively adapted to accommodate the physical limitations of size and shape imposed by a variety of biological m...
An instrument is described for transport studies in a short-circuited system which can be easily and inexpensively adapted to accommodate the physical limitations of size and shape imposed by a variety of biological membranes.
The ATPase of glycerinated sperm flagella from the sea urchin ( Strongylocentrotus franciscanus ) catalyzes the preferential exchange of water oxygens into the phosphate originating from ATP. This type of exchange, so...
The ATPase of glycerinated sperm flagella from the sea urchin ( Strongylocentrotus franciscanus ) catalyzes the preferential exchange of water oxygens into the phosphate originating from ATP. This type of exchange, so far known only for myosin-related ATPases, occurred under conditions (60 m m Mg 2+ , 1 to 10 m m Ca 2+ ) in which no inorganic phosphate-water exchange or exchange into unhydrolyzed ATP was observed. These results suggest that similar intermediates are formed by both skeletal myosin and flagellar ATPase during the cleavage of ATP, in spite of the known large differences in physical and enzymatic properties of the two contractile systems.
Ponies fasted for up to 8 days showed, both by agarose electrophoresis and preparative ultracentrifugation, the appearance of a pre-beta-migrating, very low density lipoprotein fraction in plasma. This lipoprotein dif...
详细信息
Ponies fasted for up to 8 days showed, both by agarose electrophoresis and preparative ultracentrifugation, the appearance of a pre-beta-migrating, very low density lipoprotein fraction in plasma. This lipoprotein differs from the very low density lipoprotein found in humans and rats in that it contains a relatively smaller amount of total cholesterol, 85% of which is present in the unesterified form. By the 8th day of fasting, plasma triglyceride concentrations had increased from a prefasting level of 20 mg/dl to as high as 1000 mg/dl. The increase in plasma lipid concentrations as a result of fasting was highly variable. Accumulation of plasma cholesterol and triglyceride after injection of Triton WR 1339 was not related to the degree of fasting hyperlipidemia. This suggests that the hyperlipoproteinemia of fasting may result from an impaired utilization of very low density lipoproteins.
Gingiva and dorsal skin from 25-day embryonic New Zealand white rabbits were excised and explanted as xenografts on the CAM for periods up to 10 days. Organ cultures retained their characteristic histodifferentiation ...
Gingiva and dorsal skin from 25-day embryonic New Zealand white rabbits were excised and explanted as xenografts on the CAM for periods up to 10 days. Organ cultures retained their characteristic histodifferentiation and indicated continued growth and development. Recombinants of trypsin-dissociated palate and skin epithelium and adjacent dermis illustrated advances in development. In all cases homotypic tissue isolates of epithelium or dermis did not retain their phenotypes. Recombinants of heterotypic isolated tissues indicated aninstructuve influence by the specific dermis upon the epithelium.
Objective:To study the inhibitory effect of rice bran extracts of Thai black Kam Muang and red Hawm Dawk Mali Deang on oxidative stress factors including superoxide(O2·-),nitric oxide(NO·),and inducible nitr...
详细信息
Objective:To study the inhibitory effect of rice bran extracts of Thai black Kam Muang and red Hawm Dawk Mali Deang on oxidative stress factors including superoxide(O2·-),nitric oxide(NO·),and inducible nitric oxide synthase(iNOS).Methods:Bran extracts(40%ethanol)of Kam Muang and Hawm Dawk Mali Deang were obtained and evaluated for in vitro 2-2′-azino-di-(3-ethylbenzthiazoline sulfonate)(ABTS)and NO·scavenging *** inhibitory effects on cellular O2·-and NO·were measured in phorbol 12-myristate 13-acetate-stimulated neutrophil-like HL-60 cells and lipopolysaccharidestimulated RAW264.7 macrophages,respectively,and their viability was monitored using the MTT *** effect on iNOS expression was also assessed by the Western blotting *** contents of phenolics,flavonoids,and subtypes were also ***:Hawm Dawk Mali Deang exhibited about 3.5-fold greater cellular O2·-inhibitory activity than Kam Muang[EC50 values of(23.57±4.54)and(81.98±1.45)μg/mL,respectively]in phorbol 12-myristate 13-acetate-stimulated HL-60 *** Dawk Mali Deang exhibited about 2-fold higher in vitro ABTS·+and NO·scavenging activity than Kam Muang,but it exerted cellular NO·inhibitory activity of only about 26%(undetermined EC50 value)in lipopolysaccharide-stimulated RAW264.7 ***,Kam Muang exerted potent cellular NO·inhibitory activity[EC50 value:(281.13±59.18)μg/mL]and dose-dependently decreased iNOS *** cytotoxicity of both extracts was detected in both cell *** for corresponding contents,Hawm Dawk Mali Deang contained higher contents of phenolics and flavonoids than Kam ***,Kam Muang and Hawm Dawk Mali Deang had a high content of total anthocyanins[(14.73±0.52)mg C3GE/g of extract]and total proanthocyanidins[(115.13±1.47)mg CE/g of extract],***:Based on these data,bran extracts of Thai black Kam Muang and red rice Hawm Dawk Mali Deang can help lower oxidative stress and inflammation attributed partly to O2·
Breast cancer is a perilous disease, causing a high mortality rate and posing a major public health challenge worldwide. Traditional clinical diagnosis of breast cancer typically relies on tissue biopsy, which is inva...
详细信息
We previously reported that the nucleotide complex of myosin subfragment 1, S1·єADP, exists in two states on the basis of the temperature dependence of the fluorescence decay of bound 1,N6-ethenoadenosine diphosp...
Short-circuit techniques for the study of ion transport by isolated membrane systems have been applied to defining the mechanism for calcium ion transport in the small intestine of the rat. In the absence of phosphate...
详细信息
作者：
CROISSANT, RDDepartment of Biochemistry
School of Dentistry and The Graduate Program in Cellular and Molecular Biology University of Southern California Los Angeles California 90007 USA
暂无评论