检索结果-内蒙古大学图书馆

Affordable, high-resolution bioprinting with embedded concentration gradients

Bioprinting 2021年 21卷

作者： Shen, Edwin M. McCloskey, Kara E. Graduate Program in Biological Engineering and Small-scale Technologies University of California Merced United States Department of Materials Science and Engineering University of California Merced United States

Three-dimensional (3D) bioprinting has brought major innovations to the field in its ability to fabricate cell-laden constructs that closely resemble physiological tissue. Unfortunately, many bioprinters print only one material and at limited resolutions. Improving on the resolutions, functionality, and affordability necessitates attention to both printing techniques and printers. Here, we present our results towards building an affordable, partially open-source bioprinter, ModiPrint, that enables multiple cell-material combination printing with high resolution extrusions (100 μm) of alginate. We also introduce a new technique to improve extrusion resolution in complex geometries. The results show that we were able to maintain viability of human pluripotent stem cells (hiPSCs) after high resolution printing. Lastly, we were able to generate concentration gradients of small molecules or cytokines embedded within hydrogels by combining extrusion and drop-on-demand bioprinting. We expect ModiPrint and the novel techniques in our study will assist other bioprinting researchers. © 2020 The Authors

关键词： Alginate Biomimicry Bioprinting Concentration gradients FRESH Hydrogels ModiPrint Open-source Pluripotent stem cells

来源：评论

学校读者我要写书评

暂无评论

Honeycomb microwell assay platform for generation and culture of embryoid bodies from human embryonic stem cells

Honeycomb microwell assay platform for generation and cultur...

引用

13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009

作者： Nguyen, Diep Xiang, Guangxin Pegan, Jon Park, Jason S. Nakamura, Kenta Manilay, Jennifer Conklin, Bruce R. Khine, Michelle Department of Biomedical Engineering University of California Irvine CA United States Graduate Group in Biological Engineering and Small-scale Technologies University of California Merced CA United States School of Engineering University of California Merced CA United States Department of Medicine University of California San Francisco CA United States School of Natural Sciences University of California Merced CA United States

ISBN: (纸本)9780979806421

Embryoid body (EB) formation closely recapitulates early embryonic development with respect to lineage commitment. Here we report of an ultra-rapid fabrication and culture method utilizing a laser-jet printer to generate closely arrayed honeycomb microwells of tunable sizes for the induction of uniform EBs from single cell suspension. By printing various microwell patterns onto pre-stressed polystyrene sheets, and through heat induced shrinking, high aspect micromolds are generated. Notably, we achieve rounded bottom polydimethylsiloxane (PDMS) wells not easily achievable with standard microfabrication methods, but critical to achieve spherical EBs. Furthermore, by controlling the size of the microwells we can control the initial size of the cell aggregates. © 2009 CBMS.

关键词： Polydimethylsiloxane

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还