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Tuberculosis infection and lung adenocarcinoma: Mendelian randomization and pathway analysis of genome-wide association study data from never-smoking Asian women

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Genomics 2020年第2期112卷 1223-1232页

作者： Jason Y Y Wong Han Zhang Chao A Hsiung Kouya Shiraishi Kai Yu Keitaro Matsuo Maria Pik Wong Yun-Chul Hong Jiucun Wang Wei Jie Seow Zhaoming Wang Minsun Song Hee Nam Kim I-Shou Chang Nilanjan Chatterjee Wei Hu Chen Wu Tetsuya Mitsudomi Wei Zheng Jin Hee Kim Adeline Seow Neil E Caporaso Min-Ho Shin Lap Ping Chung She-Juan An Ping Wang Yang Yang Hong Zheng Yasushi Yatabe Xu-Chao Zhang Young Tae Kim Qiuyin Cai Zhihua Yin Young-Chul Kim Bryan A Bassig Jiang Chang James Chung Man Ho Bu-Tian Ji Yataro Daigo Hidemi Ito Yukihide Momozawa Kyota Ashikawa Yoichiro Kamatani Takayuki Honda H Dean Hosgood Hiromi Sakamoto Hideo Kunitoh Koji Tsuta Shun-Ichi Watanabe Michiaki Kubo Yohei Miyagi Haruhiko Nakayama Shingo Matsumoto Masahiro Tsuboi Koichi Goto Jianxin Shi Lei Song Xing Hua Atsushi Takahashi Akiteru Goto Yoshihiro Minamiya Kimihiro Shimizu Kazumi Tanaka Fusheng Wei Fumihiko Matsuda Jian Su Yeul Hong Kim In-Jae Oh Fengju Song Wu-Chou Su Yu-Min Chen Gee-Chen Chang Kuan-Yu Chen Ming-Shyan Huang Li-Hsin Chien Yong-Bing Xiang Jae Yong Park Sun-Seog Kweon Chien-Jen Chen Kyoung-Mu Lee Batel Blechter Haixin Li Yu-Tang Gao Biyun Qian Daru Lu Jianjun Liu Hyo-Sung Jeon Chin-Fu Hsiao Jae Sook Sung Ying-Huang Tsai Yoo Jin Jung Huan Guo Zhibin Hu Wen-Chang Wang Charles C Chung Laurie Burdett Meredith Yeager Amy Hutchinson Sonja I Berndt Wei Wu Herbert Pang Yuqing Li Jin Eun Choi Kyong Hwa Park Sook Whan Sung Li Liu C H Kang Meng Zhu Chung-Hsing Chen Tsung-Ying Yang Jun Xu Peng Guan Wen Tan Chih-Liang Wang Michael Hsin Ko-Yung Sit James Ho Ying Chen Yi Young Choi Jen-Yu Hung Jun Suk Kim Ho Il Yoon Chien-Chung Lin In Kyu Park Ping Xu Yuzhuo Wang Qincheng He Reury-Perng Perng Chih-Yi Chen Roel Vermeulen Junjie Wu Wei-Yen Lim Kun-Chieh Chen Yao-Jen Li Jihua Li Hongyan Chen Chong-Jen Yu Li Jin Tzu-Yu Chen Shih-Sheng Jiang Jie Liu Taiki Yamaji Belynda Hicks Kathleen Wyatt Shengchao A Li Juncheng Dai Hongxia Ma Guangfu Jin Bao Song Zhehai Wang Sensen Cheng Xuelian Li Yangwu Ren Ping Cui Motoki Iwasaki Taichi Shimazu Shoichiro Tsugane Junjie Division of Cancer Epidemiology and Genetics National Cancer Institute Bethesda MD USA. Electronic address: jason.wong@nih.gov. Division of Cancer Epidemiology and Genetics National Cancer Institute Bethesda MD USA. Institute of Population Health Sciences National Health Research Institutes Zhunan Taiwan. Division of Genome Biology National Cancer Center Research Institute Tokyo Japan. Division of Cancer Epidemiology and Prevention Aichi Cancer Center Research Institute Nagoya Japan Department of Cancer Epidemiology Nagoya University Graduate School of Medicine Nagoya Japan. Department of Pathology Queen Mary Hospital The University of Hong Kong Hong Kong. Department of Preventive Medicine Seoul National University College of Medicine Seoul Republic of Korea. Ministry of Education Key Laboratory of Contemporary Anthropology School of Life Sciences Fudan University Shanghai China State Key Laboratory of Genetic Engineering School of Life Sciences Fudan University Shanghai China. Saw Swee Hock School of Public Health National University of Singapore Singapore. Cancer Genomics Research Laboratory Leidos Biomedical Research Inc Gaithersburg MD USA Department of Computational Biology St. Jude Children's Research Hospital Memphis TN USA. Division of Cancer Epidemiology and Genetics National Cancer Institute Bethesda MD USA Department of Statistics Sookmyung Women's University Seoul Republic of Korea. Department of Preventive Medicine Chonnam National University Medical School Gwangju Republic of Korea. National Institute of Cancer Research National Health Research Institutes Zhunan Taiwan. Bloomberg School of Public Health Johns Hopkins University Baltimore MD USA. Department of Etiology & Carcinogenesis and State Key Laboratory of Molecular Oncology Cancer Institute and Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China. Division of Thoracic Surgery Kinki University School of Medicine Sayama Japan. Division of

We investigated whether genetic susceptibility to tuberculosis (TB) influences lung adenocarcinoma development among never-smokers using TB genome-wide association study (GWAS) results within the Female Lung Cancer Consortium in Asia. Pathway analysis with the adaptive rank truncated product method was used to assess the association between a TB-related gene-set and lung adenocarcinoma using GWAS data from 5512 lung adenocarcinoma cases and 6277 controls. The gene-set consisted of 31 genes containing known/suggestive associations with genetic variants from previous TB-GWAS. Subsequently, we followed-up with Mendelian Randomization to evaluate the association between TB and lung adenocarcinoma using three genome-wide significant variants from previous TB-GWAS in East Asians. The TB-related gene-set was associated with lung adenocarcinoma (p = 0.016). Additionally, the Mendelian Randomization showed an association between TB and lung adenocarcinoma (OR = 1.31, 95% CI: 1.03, 1.66, p = 0.027). Our findings support TB as a causal risk factor for lung cancer development among never-smoking Asian women.

关键词： Lung adenocarcinoma Lung cancer Mendelian randomization Pathway analysis Tuberculosis

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Neural stem cells attacked by Zika virus

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细胞研究（英文版） 2016年第7期26卷 753-754页

作者： Ha Nam Nguyen Xu yu Qian Hong jun Song Guo-li Ming Institute for Cell Engineering Johns Hopkins University School of Medicine Baltimore MD 21205 USA Department of Neurology Johns Hopkins University School of Medicine Baltimore MD 21205 USA Institute for Cell Engineering Johns Hopkins University School of Medicine Baltimore MD 21205 USA Biomedical Engineering Graduate Program Johns Hopkins University School of Medicine Baltimore MD 21205 USA Institute for Cell Engineering Johns Hopkins University School of Medicine Baltimore MD 21205 USA Department of Neurology Johns Hopkins University School of Medicine Baltimore MD 21205 USA The Solomon Snyder Department of Neuroscience Johns Hopkins University School of Medicine Baltimore MD 21205 USA Institute for Cell Engineering Johns Hopkins University School of Medicine Baltimore MD 21205 USA Department of Neurology Johns Hopkins University School of Medicine Baltimore MD 21205 USA The Solomon Snyder Department of Neuroscience Johns Hopkins University School of Medicine Baltimore MD 21205 USA Department of Psychiatry and Behavioral Sciences Johns Hopkins University School of Medicine Baltimore MD 21205 USA

The current outbreak of Zika virus-associated diseases in South America and its threat to spread to other parts of the world has emerged as a global health *** from cell and animal models to understand how Zika virus ... 详细信息

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Corrigendum to "A mixed-effects model approach for the statistical analysis of vocal fold viscoelastic shear properties" [J. Mech. Behav. Biomed. Mater. 75 (2017) 477-485]

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Journal of the mechanical behavior of biomedical materials 2018年 79卷 114页

作者： Chet C Xu Roger W Chan Han Sun Xiaowei Zhan Department of Otolaryngology-Head and Neck Surgery University of Texas Southwestern Medical Center Dallas TX 75390 USA Graduate Program in Biomedical Engineering University of Texas Southwestern Medical Center Dallas TX 75390 USA. Electronic address: che.xu@utsouthwestern.edu. Department of Speech Language Pathology National Taipei University of Nursing and Health Sciences Taipei Taiwan. Department of Quantitative Health Sciences the Cleveland Clinic Foundation Cleveland OH 44195 USA Department of Population and Quantitative Health Sciences Case Western Reserve University Cleveland OH 44106 USA. Department of Clinical Science University of Texas Southwestern Medical Center Dallas TX 75390 USA.

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Human Bone Xenografts: from Preclinical Testing for Regenerative Medicine to Modeling of Diseases

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Current Molecular Biology Reports 2016年第3期2卷 158-170页

作者： Mark Seow Khoon Chong Chaolemeng Bao King Pan Ng Jing Lim Jerry Kok Yen Chan Division of Bioengineering School of Chemical and Biomedical Engineering Nanyang Technological University Singapore Singapore Department of Obstetrics and Gynaecology Yong Loo Lin School of Medicine National University of Singapore Singapore Singapore Cancer and Stem Cell Biology Program Duke-NUS Graduate Medical School Singapore Singapore Department of Reproductive Medicine KK Women’s and Children’s Hospital Singapore Singapore

Xenografting involves the transplantation of human tissue or cells into animal models and is an important tool for regenerative medicine research. Implantation of engineered human bone tissues into animal models, for example, is performed in preclinical evaluations of product safety and efficacy. With the advent of improved experimental methodologies, these models are further being exploited to interrogate molecular mechanisms and physiological interactions in vivo. In parallel to these developments, patient-derived xenograft murine models of cancer are increasingly being studied for various applications in cancer research and therapy; it follows that xenograft models in tissue engineering may be adapted for such approaches. In this review, we first discuss the development of human bone xenograft models to recapitulate physiological states in regenerative medicine. Subsequently, we discuss the use of these techniques for applications in modeling pathological states in skeletal oncology, namely, hematopoietic malignancies, bone metastatic disease, and primary bone malignancy.

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Enhancing TA grading of technical writing: A look back to better understand the future 122

Enhancing TA grading of technical writing: A look back to be...

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2015 122nd ASEE Annual Conference and Exposition

作者： Kecskemety, Krista M. Theiss, Andrew H. Kajfez, Rachel Louis Ohio State University Engineering Education Innovation Center United States Ohio State University Biomedical Engineering Graduate Program United States Engineering Education Innovation Center Department of Civil Environmental and Geodetic Engineering Ohio State University United States

Technical writing is an important skill in all engineering disciplines. Many first-year engineering programs (FYEPs) include technical writing as one of their core course components to begin to instill the importance of and to develop this skill early in aspiring engineers. In order to assess student learning and provide feedback on technical writing, proper grading of these assignments is essential. This paper presents the preliminary assessment results of a new grading training program for teaching assistants (TAs) in a FYEP that was implemented at a large land grant institution while situating the findings in past research related to the grading of writing assignments and preparing TAs. This paper summarizes the approach used to enhance the grading of TAs. We supplement this discussion with quantitative and qualitative assessment results. Finally, we provide recommendations or "Tricks of the Trade" for TAs who may be grading technical writing and those who are responsible for training TAs for this type of grading. © American Society for engineering Education, 2015.

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Current methods to manufacture human platelet lysates for cell therapy and tissue engineering: possible trends in product safety and standardization

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ISBT Science Series 2016年第1期12卷

作者： M.L. Chou T. Burnouf Graduate Institute of Medical Sciences College of Medicine Taipei Medical University Taipei Taiwan Graduate Institute of Biomedical Materials and Tissue Engineering College of Biomedical Engineering Taipei Medical University Taipei Taiwan International PhD program in Biomedical Engineering College of Biomedical Engineering Taipei Medical University Taipei Taiwan

Human platelet lysates (HPLs), obtained from therapeutic-grade platelet concentrates (PC), are rich in growth factors and other bioactive molecules. HPL is increasingly evaluated as a substitute for foetal bovine serum (FBS), to likely become the next ‘gold standard’ supplement of growth media for clinical-grade ex vivo propagation of human cells. HPL avoids the risks of immunological reactions against xenoproteins. HPL quality and safety benefit from the pathogen safety measures in place for producing PC for transfusion. Numerous studies support that HPL-expanded mesenchymal stromal cells show improved proliferation and decreased doubling time than when using FBS-supplemented media and maintain characteristic immunophenotype, differentiation capacity and T-cell immunosuppressive effect. Several methods are used to prepare HPL; they differ in the selection and formulation of the starting PC, the consideration or not for blood groups, the pool size, the membrane lysis procedure, the depletion of fibrinogen, and the implementation or not of a pathogen inactivation step on PC materials or during manufacture. Allogeneic ‘off-the-shelf’ pooled HPLs, made from approximately fifty, or more, PC, are currently produced by blood establishments and/or available through commercial suppliers. Improved HPL standardization and safety profile, through larger pools and dedicated viral inactivation and removal treatments, is expected in the next few years, broadening applications in cell therapy and regenerative medicine. Systematic studies of the impact of the mode of production of HPL on the isolation and expansion of various human cell types should be encouraged.

关键词： cellular therapy mesenchymal stromal cells platelet components

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Author Correction: The Rho GTPase Rnd1 suppresses mammary tumorigenesis and EMT by restraining Ras-MAPK signalling

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Nature cell biology 2019年第4期21卷 534页

作者： Tomoyo Okada Surajit Sinha Ilaria Esposito Gaia Schiavon Miguel A López-Lago Wenjing Su Christine A Pratilas Cristina Abele Jonathan M Hernandez Masahiro Ohara Morihito Okada Agnes Viale Adriana Heguy Nicholas D Socci Anna Sapino Venkatraman E Seshan Stephen Long Giorgio Inghirami Neal Rosen Filippo G Giancotti Cell Biology Program Sloan-Kettering Institute for Cancer Research Memorial Sloan-Kettering Cancer Center New York New York 10065 USA. Department of Surgery Memorial Sloan-Kettering Cancer Center New York 10065 USA. Breast Unit Royal Marsden Hospital London SW3 6JJ UK. Department of Surgery and Center for Cell Engineering Memorial Sloan-Kettering Cancer Center New York 10065 USA. Molecular Pharmacology and Chemistry Program Sloan-Kettering Institute for Cancer Research Memorial Sloan-Kettering Cancer Center New York New York 10065 USA. Department of Pediatrics Memorial Hospital Memorial Sloan-Kettering Cancer Center New York New York 10065 USA. The Sidney Kimmel Comprehensive Cancer Center Johns Hopkins University School of Medicine Baltimore Maryland 21287 USA. Department of Biomedical Sciences and Human Oncology Center of Experimental Medicine and Research University of Torino Torino 10126 Italy. Department of Surgery Memorial Hospital Memorial Sloan-Kettering Cancer Center New York New York 10065 USA. Department of Surgical Oncology Research Institute for Radiation Biology and Medicine Graduate School of Biomedical Sciences Hiroshima University Hiroshima 734-8551 Japan. Genomics Core Facility Memorial Sloan-Kettering Cancer Center New York New York 10065 USA. Geoffrey Beene Translational Oncology Core Facility Memorial Sloan-Kettering Cancer Center New York New York 10065 USA. Department of Pathology Office for Collaborative Science New York University New York 10016 USA. Bioinformatics Core Facility Memorial Sloan-Kettering Cancer Center New York New York 10065 USA. Department of Medical Sciences Center of Experimental Medicine and Research University of Torino Torino 10126 Italy. Department of Epidemiology and Biostatistics Memorial Hospital Memorial Sloan-Kettering Cancer Center New York New York 10065 USA. Structural Biology Program Sloan-Kettering Institute for Cancer Research Memorial Sloan-Kettering Cancer Center New Y

In the version of this Article originally published the same blot was inadvertently presented as both p-Rb and Cyclin A in Fig. 2a. This blot corresponds to the p-Rb panel, as can be seen in the unprocessed version of these blots in Supplementary Fig. 9. The corrected version of the panel is shown below, together with a completely uncropped image of both blots. In addition, in the 'Viral transduction' section of the Methods, the pLKO.1 plasmids encoding short hairpin RNAs targeting human Rnd1 were incorrectly listed as clones TRCN0000018338 and TRCN0000039977. The correct clone numbers are TRCN0000047434 and TRCN0000047435.

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Correction to: 2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales

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Archives of virology 2021年第12期166卷 3567-3579页

作者： Jens H Kuhn Scott Adkins Bernard R Agwanda Rim Al Kubrusli Sergey V Alkhovsky Gaya K Amarasinghe Tatjana Avšič-Županc María A Ayllón Justin Bahl Anne Balkema-Buschmann Matthew J Ballinger Christopher F Basler Sina Bavari Martin Beer Nicolas Bejerman Andrew J Bennett Dennis A Bente Éric Bergeron Brian H Bird Carol D Blair Kim R Blasdell Dag-Ragnar Blystad Jamie Bojko Wayne B Borth Steven Bradfute Rachel Breyta Thomas Briese Paul A Brown Judith K Brown Ursula J Buchholz Michael J Buchmeier Alexander Bukreyev Felicity Burt Carmen Büttner Charles H Calisher Mengji Cao Inmaculada Casas Kartik Chandran Rémi N Charrel Qi Cheng Yuya Chiaki Marco Chiapello Il-Ryong Choi Marina Ciuffo J Christopher S Clegg Ian Crozier Elena Dal Bó Juan Carlos de la Torre Xavier de Lamballerie Rik L de Swart Humberto Debat Nolwenn M Dheilly Emiliano Di Cicco Nicholas Di Paola Francesco Di Serio Ralf G Dietzgen Michele Digiaro Olga Dolnik Michael A Drebot J Felix Drexler William G Dundon W Paul Duprex Ralf Dürrwald John M Dye Andrew J Easton Hideki Ebihara Toufic Elbeaino Koray Ergünay Hugh W Ferguson Anthony R Fooks Marco Forgia Pierre B H Formenty Jana Fránová Juliana Freitas-Astúa Jingjing Fu Stephanie Fürl Selma Gago-Zachert George Fú Gāo María Laura García Adolfo García-Sastre Aura R Garrison Thomas Gaskin Jean-Paul J Gonzalez Anthony Griffiths Tony L Goldberg Martin H Groschup Stephan Günther Roy A Hall John Hammond Tong Han Jussi Hepojoki Roger Hewson Jiang Hong Ni Hong Seiji Hongo Masayuki Horie John S Hu Tao Hu Holly R Hughes Florian Hüttner Timothy H Hyndman M Ilyas Risto Jalkanen Dàohóng Jiāng Gilda B Jonson Sandra Junglen Fujio Kadono Karia H Kaukinen Michael Kawate Boris Klempa Jonas Klingström Gary Kobinger Igor Koloniuk Hideki Kondō Eugene V Koonin Mart Krupovic Kenji Kubota Gael Kurath Lies Laenen Amy J Lambert Stanley L Langevin Benhur Lee Elliot J Lefkowitz Eric M Leroy Shaorong Li Longhui Li Jiànróng Lǐ Huazhen Liu Igor S Lukashevich Piet Maes William Marciel de Souza Marco Marklewitz Sergio H Marshall Shin-Yi L Marzano Se Integrated Research Facility at Fort Detrick National Institute of Allergy and Infectious Diseases National Institutes of Health Frederick MD USA. kuhnjens@mail.nih.gov. United States Department of Agriculture Agricultural Research Service US Horticultural Research Laboratory Fort Pierce FL USA. Zoology Department National Museums of Kenya Nairobi Kenya. Jomo Kenyatta University of Agriculture & Technology Nairobi Kenya. Division Phytomedicine Faculty of Life Sciences Humboldt-Universität zu Berlin Berlin Germany. D.I. Ivanovsky Institute of Virology of N.F. Gamaleya National Center on Epidemiology and Microbiology of Ministry of Health of Russian Federation Moscow Russia. Department of Pathology and Immunology Washington University School of Medicine St. Louis MO USA. Faculty of Medicine University of Ljubljana Ljubljana Slovenia. Centro de Biotecnología y Genómica de Plantas Universidad Politécnica de Madrid-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria Campus de Montegancedo Pozuelo de Alarcón Madrid Spain. Departamento de Biotecnología-Biología Vegetal Escuela Técnica Superior de Ingeniería Agronómica Alimentaria y de Biosistemas Universidad Politécnica de Madrid Madrid Spain. Center for Ecology of Infectious Diseases Department of Infectious Diseases Department of Epidemiology and Biostatistics Insitute of Bioinformatics University of Georgia Athens GA USA. Institute of Novel and Emerging Infectious Diseases Friedrich-Loeffler-Institut Federal Research Institute for Animal Health Greifswald-Insel Riems Germany. Department of Biological Sciences Mississippi State University Mississippi State MS USA. Center for Microbial Pathogenesis Institute for Biomedical Sciences Georgia State University Atlanta GA USA. Edge BioInnovation Consulting and Mgt Frederick MD USA. Institute of Diagnostic Virology Friedrich-Loeffler-Institut Greifswald-Insel Riems Germany. UFyMA INTA-CONICET Cordoba Argentina. Genomics and Bioinformatics D

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Author Correction: Behavior of mice aboard the International Space Station

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Scientific reports 2019年第1期9卷 10154页

作者： April E Ronca Eric L Moyer Yuli Talyansky Moniece Lowe Shreejit Padmanabhan Sungshin Choi Cynthia Gong Samuel M Cadena Louis Stodieck Ruth K Globus NASA Ames Research Center Space Biosciences Division Moffett Field CA 94035 USA. april.e.ronca@nasa.gov. Wake Forest School of Medicine Obstetrics and Gynecology Winston-Salem NC 27101 USA. april.e.ronca@nasa.gov. Blue Marble Space Institute of Science Seattle WA 98154 USA. Utrecht University Graduate School of Life Sciences Regenerative Medicine and Technology Program Universiteitsweg 98 3584 CG Utrecht The Netherlands. Universities Space Research Association NASA Ames Research Center Moffett Field CA 94035 USA. San Jose State University San Jose CA 95192 USA. Keck School of Medicine of the University of Southern California Department of Molecular Microbiology and Immunology 2011 Zonal Avenue Los Angeles CA 90033 USA. Duke Empirical Inc. 2829 Mission St Santa Cruz CA 95060 USA. KBRwyle NASA Ames Research Center Moffett Field CA 94035 USA. Novartis Institutes for Biomedical Research Cambridge MA 02139 USA. BioServe Space Technologies Department of Aerospace Engineering Sciences University of Colorado Boulder CO 80302 USA. NASA Ames Research Center Space Biosciences Division Moffett Field CA 94035 USA.

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.

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(OA32) Chronic Radiation-Associated Dysphagia in Oropharyngeal Cancer Survivors: Towards Age-Adjusted Dose Constraints for Deglutitive Muscles

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International Journal of Radiation Oncology*Biology*Physics 2018年第2期101卷 e14-e14页

作者： Kaitlin Christopherson Alokananda Ghosh Abdallah Sherif Radwan Mohamed Mona Jomaa G. Brandon Gunn Liz Marai Guadalupe Canahuate David Vock Timothy Dale Jayashree Kalpathy-Cramer Jay Messer Adam S. Garden Hesham Elhalawani Steven J. Frank Jan Lewin Stephen Y. Lai Beth M. Beadle William H. Morrison Clifton D. Fuller Department of Radiation Oncology The University of Texas MD Anderson Cancer Center Houston TX USA Department of Biostatistics University of Texas School of Public Health Houston TX USA Department of Clinical Oncology University of Alexandria Alexandria Egypt Head and Neck Surgery The University of Texas MD Anderson Cancer Center Houston TX USA Department of Computer Science University of Pittsburgh Pittsburgh PA Thoracic & Head and Neck Oncology The University of Texas MD Anderson Cancer Center Houston TX USA Department of Electrical and Computer Engineering The University of Iowa Iowa City IA Division of Biostatistics School of Public Health University of Minnesota Minneapolis MN Baylor College of Medicine Houston TX USA Athinoula A. Martinos Center for Biomedical Imaging/Massachusetts General Hospital/Massachusetts Institute of Technology Charlestown MA USA Department of Radiation Oncology The University of Texas Medical Branch Galveston TX USA Department of Radiation Oncology Stanford University Palo Alto CA USA Medical Physics Program The University of Texas Graduate School of Biomedical Sciences Houston TX USA

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