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Downsizing and soft X-ray tomography for cellular uptake of interpenetrated metal-organic frameworks

Journal of Materials Chemistry B 2024年第25期12卷 6079-6090页

作者： Yu, Yu-Sheng Liang, Yung-Yi Hsieh, Chia-Chun Lin, Zi-Jing Cheng, Po-Hsiu Cheng, Chih-Chan Chen, Shu-Ping Lai, Lee-Jene Wu, Kevin C.-W. National Taiwan University Department of Chemical Engineering Taipei Taiwan National Health Research Institute Institute of Biomedical Engineering and Nanomedicine Miaoli Taiwan Hsinchu Taiwan International Graduate Program of Molecular Science and Technology Taiwan International Graduate Program Academia Sinica Taipei Taiwan National Taiwan University Taipei Taiwan

Metal-organic frameworks (MOFs) are porous materials with potential in biomedical applications such as sensing, drug delivery, and radiosensitization. However, how to tune the properties of the MOFs for such applications remains challenging. Herein, we synthesized two MOFs, Zr-PEB and Hf-PEB. Zr-PEB can be classified as porous interpenetrated zirconium frameworks (PIZOFs) and Hf-PEB is its analogue. We controlled their sizes while maintaining their crystal structure by employing a coordination modulation strategy. They were designed to serve as sensitizer for X-ray therapy and as potential drug carriers. Comprehensive characterizations of the MOFs' properties have been conducted, and the in vitro biological impacts have been studied. Since viability assay showed that Hf-PEB was more biocompatible compared to Zr-PEB, the cellular uptake of Hf-PEB by cells was evaluated using both fluorescence microscopy and soft X-ray tomography (SXT), and the three-dimensional structure of Hf-PEB in cells was observed. The results revealed the potential of Zr-PEB and Hf-PEB as nanomaterials for biomedical applications and demonstrated that SXT is an effective tool to assist the development of such materials. © 2024 The Royal Society of Chemistry.

关键词： Organometallics

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Treatment of spinal cord injury with biomaterials and stem cell therapy in non-human primates and humans

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Neural Regeneration Research 2025年第2期20卷 343-353页

作者： Ana Milena Silva Olaya Fernanda Martins Almeida Ana Maria Blanco Martinez Suelen Adriani Marques PhD Program in Pathological Anatomy Federal University of Rio de JaneiroRio de JaneiroBrazil Biomedical Science Institute Federal University of Rio de JaneiroRio de JaneiroBrazil Graduate Program in Pathological Anatomy Federal University of Rio de JaneiroRio de JaneiroBrazil Graduate Program in Pathological Anatomy(PPGAP/UFRJ) Department of Neurobiology/Institute of BiologyCampus do GragoatáNiteróiRio de JaneiroBrazil

Spinal cord injury results in the loss of sensory,motor,and autonomic functions,which almost always produces permanent physical ***,in the search for more effective treatments than those already applied for years,which are not entirely efficient,researches have been able to demonstrate the potential of biological strategies using biomaterials to tissue manufacturing through bioengineering and stem cell therapy as a neuroregenerative approach,seeking to promote neuronal recovery after spinal cord *** of these strategies has been developed and meticulously evaluated in several animal models with the aim of analyzing the potential of interventions for neuronal repair and,consequently,boosting functional *** the majority of experimental research has been conducted in rodents,there is increasing recognition of the importance,and need,of evaluating the safety and efficacy of these interventions in non-human primates before moving to clinical trials involving therapies potentially promising in *** article is a literature review from databases(PubMed,science Direct,Elsevier,Scielo,Redalyc,Cochrane,and NCBI)from 10 years ago to date,using keywords(spinal cord injury,cell therapy,non-human primates,humans,and bioengineering in spinal cord injury).From 110 retrieved articles,after two selection rounds based on inclusion and exclusion criteria,21 articles were ***,this review arises from the need to recognize the experimental therapeutic advances applied in non-human primates and even humans,aimed at deepening these strategies and identifying the advantages and influence of the results on extrapolation for clinical applicability in humans.

关键词： bioengineering biomaterials cell therapy humans non-human primates spinal cord injury stem cell therapy

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Development and application of 4D light-field and quantum imaging technologies

Development and application of 4D light-field and quantum im...

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biomedical Imaging and Sensing Conference 2023

作者： Sugi, Takuma Program of Biomedical Science Graduate School of Integrated Sciences for Life Hiroshima University Hiroshima Higashi-Hiroshima739-0046 Japan

ISBN: (纸本)9781510663435

Behavior is a consequence of computation in a neural circuit composed of massive millisecond neural firings that undergo in 4D space. We recently found that the nematode Caenorhabditis elegans exhibits collective pattern formations. To examine the neural computation mechanism in this collective behavior, we are developing a new light-field microscopy. Light-field microscopy is a computational imaging technology that enables scan-less, single-shot acquisition of a 3D volumetric image by postprocessing 2D camera snapshots recorded via a microlens array. Here, we show C. elegans collective behavior and high-resolution light-field microscopy. We performed single-shot 4D brain-wide imaging of a freely behaving C. elegans with high temporal resolution and 3D volumetric imaging of mouse hippocampus amyloid plaques. Finally, we also combined high-resolution light-field microscopy with a quantum sensor, and demonstrated an ultrasensitive 4D physical parameter sensing in Hela cells and in vivo. © 2023 SPIE.

关键词： Computational Imaging

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Fabrication of versatile poly(xylitol sebacate)-co-poly(ethylene glycol) hydrogels through multifunctional crosslinkers and dynamic bonds for wound healing

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Acta Biomaterialia 2023年 170卷 344-359页

作者： Yeh, Ying-Yu Lin, Yi-Yun Wang, Ting-Teng Yeh, Yu-Jia Chiu, Ting-Hsiang Wang, Reuben Bai, Meng-Yi Yeh, Yi-Cheun Institute of Polymer Science and Engineering National Taiwan University Taipei Taiwan Institute of Biomedical Engineering National Taiwan University Taipei Taiwan Biomedical Engineering Program Graduate Institute of Applied Science and Technology National Taiwan University of Science and Technology Taipei Taiwan Institute of Food Safety and Health National Taiwan University Taipei Taiwan Graduate Institute of Biomedical Engineering National Taiwan University of Science and Technology Taipei Taiwan Adjunct Appointment to the Department of Biomedical Engineering National Defense Medical Center Taipei Taiwan Program National Taiwan University Taipei Taiwan GIP-TRIAD Master's Degree in Agro-Biomedical Science National Taiwan University Taipei Taiwan

Poly(polyol sebacate) (PPS) polymer family has been recognized as promising biomaterials for biomedical applications with their characteristics of easy production, elasticity, biodegradation, and cytocompatibility. Poly(xylitol sebacate)-co-poly(ethylene glycol) (PXS-co-PEG) has been developed to fabricate PPS-based hydrogels;however, current PXS-co-PEG hydrogels presented limited properties and functions due to the limitations of the crosslinkers and crosslinking chemistry used in the hydrogel formation. Here, we fabricate a new type of PXS-co-PEG hydrogels through the use of multifunctional crosslinkers as well as dynamic bonds. In our design, polyethyleneimine-polydopamine (PEI-PDA) macromers are utilized to crosslink aldehyde-functionalized PXS-co-PEG (APP) through imine bonds and hydrogen bonds. PEI-PDA/APP hydrogels present multiple functional properties (e.g., fluorescent, elastomeric, biodegradable, self-healing, bioadhesive, antioxidant, and antibacterial behaviors). These properties of PEI-PDA/APP hydrogels can be fine-tuned by changing the PDA grafting degrees in the PEI-PDA crosslinkers. Most importantly, PEI-PDA/APP hydrogels are considered promising wound dressings to promote tissue remodeling and prevent bacterial infection in vivo. Taken together, PEI-PDA/APP hydrogels have been demonstrated as versatile biomaterials to provide multiple tailorable properties and desirable functions to expand the utility of PPS-based hydrogels for advanced biomedical applications. Statement of significance: Various strategies have been developed to fabricate poly(polyol sebacate) (PPS)-based hydrogels. However, current PPS-based hydrogels present limited properties and functions due to the limitations of the crosslinkers and crosslinking chemistry used in the hydrogel formation. This work describes that co-engineering crosslinkers and interfacial crosslinking is a promising approach to synthesizing a new type of poly(xylitol sebacate)-co-poly(ethylene glycol) (PXS-co-

关键词： Polyols

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GeoRefineNet: A Multistage Framework for Enhanced Cephalometric Landmark Detection in CBCT Images Using 3D Geometric Information 17th

GeoRefineNet: A Multistage Framework for Enhanced Cephalome...

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17th Asian Conference on Computer Vision, ACCV 2024

作者： Viriyasaranon, Thanaporn Ma, Serie Choi, Jang-Hwan Department of Computational Medicine Graduate Program in System Health Science and Engineering Ewha Womans University Seoul Korea Republic of Division of Mechanical and Biomedical Engineering Graduate Program in System Health Science and Engineering Ewha Womans University Seoul Korea Republic of Department of Artificial Intelligence Ewha Womans University Seoul Korea Republic of

ISBN: (纸本)9789819609000

The precise detection of cephalometric landmarks on two-dimensional (2D) radiographs or three-dimensional (3D) computed tomography (CT) images is a fundamental step in various medical fields, especially in research on orthodontics and maxillofacial surgery. Deep learning-based detectors have demonstrated remarkable accuracy in 2D cephalometric analysis, whereas conventional single-view approaches are limited by their reliance on information from a single perspective. This study proposes GeoRefineNet, a novel multistage framework that leverages information from multiple CT scans acquired at various angles. By incorporating geometric knowledge through a 3D heatmap reconstruction process, GeoRefineNet improves robustness, accuracy, and adaptability to various cephalometric configurations. The proposed framework predicts 3D landmark positions on CT images, effectively addressing challenges associated with high-dimensional input data and limited training examples. GeoRefineNet surpasses the existing state-of-the-art models in the 2D and 3D domains, as demonstrated by its superior performance on numerical and clinical datasets. These findings indicate that GeoRefineNet offers a promising avenue for improving the accuracy and reliability of cephalometric landmark detection fostering further advances in clinical diagnosis and treatment planning. Our code is available at https://***/Thanaporn09/***. © The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2025.

关键词： Computerized tomography

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Compact Model Build Upon Dynamic Weighting Artificial Neural Network Approach for Complementary Field Effect Transistors

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IEEE Transactions on Electron Devices 2024年第1期71卷 246-253页

作者： Butola, Rajat Li, Yiming Kola, Sekhar Reddy National Yang Ming Chiao Tung University Parallel and Scientific Computing Laboratory Electrical Engineering and Computer Science International Graduate Program Hsinchu300093 Taiwan Institute of Pioneer Semiconductor Innovation The Institute of Artificial Intelligence Innovation National Yang Ming Chiao Tung University Parallel and Scientific Computing Laboratory Electrical Engineering and Computer Science International Graduate Program The Institute of Communications Engineering the Institute of Biomedical Engineering Department of Electronics and Electrical Engineering Hsinchu300093 Taiwan

In this work, a dynamic weighting-artificial neural network (DW-ANN) methodology is presented for quick and automated compact model (CM) generation. It takes advantage of both TCAD simulations for high accuracy and SPICE simulations for cost-effectiveness. This methodology is developed for gate-all-around (GAA) silicon (Si) nanosheet (NS) complementary field effect transistor (CFET), a potential candidate for future CMOS technology due to its innate properties. The critical process variation (PV) sources that severely degrade the CFET performance are estimated using DW-ANN. It reduces the computation cost and predicts the effects of PV sources with less than 1% error. Furthermore, a compact DW-ANN-based Verilog-A model has been developed that captures the dc as well as transient behavior accurately for circuit-level analysis. CFET-based circuits such as inverter, 6T-static random access memory (SRAM), and ring oscillator (RO) are characterized and implemented using DW-ANN-based CM. The overall average error of the model is reported as less than 2%. Therefore, the proposed device and circuit modeling approach provides a feasible solution for the rapid compact modeling of new emerging devices with good convergence and high accuracy. © 1963-2012 IEEE.

关键词： Neural networks

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Multiplexed Nanoscopy via Buffer Exchange

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ACS Nano 2024年第34期18卷 23445-23456页

作者： Chang, Ting-Jui Ben Yang, T. Tony Department of Electrical Engineering National Taiwan University Taipei10617 Taiwan Graduate Institute of Biomedical Electronics and Bioinformatics National Taiwan University Taipei10617 Taiwan Department of Physics National Taiwan University Taipei10617 Taiwan Nano Science and Technology Program Taiwan International Graduate Program Academia Sinica and National Taiwan University Taipei10617 Taiwan

Understanding cellular functions, particularly in their intricate complexity, can greatly benefit from the spatial mapping of diverse molecules through multitarget single-molecule localization microscopy (SMLM). Existing methodologies, primarily restricting the encoding dimensions to color and lifetime or requiring cyclic staining, often involve broad chromatic detection, specialized optical configurations, or sophisticated labeling techniques. Here, we propose a simple approach called buffer-exchange stochastic optical reconstruction microscopy (beSTORM), which introduces an additional dimension to differentiate between single molecules irrespective of their spectral properties. This method leverages the distinguishable photoblinking responses to distinct buffer conditions, offering a straightforward yet effective means of fluorophore discrimination. Through buffer exchanges, beSTORM achieves multitarget SMLM imaging with minimal crosstalk. Direct integration with expansion microscopy (ExM) demonstrates its capability to resolve up to six proteins at the molecular level within a single emission color without chromatic aberration. Overall, beSTORM presents a highly compatible imaging platform, promising significant advancements in highly multiplexed nanoscopy for exploring multiple targets in biological systems with nanoscale precision. © 2024 The Authors. Published by American Chemical Society.

关键词： Stochastic systems

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Molecular Mechanism of pH-Induced Protrusion Configuration Switching in Piscine Betanodavirus Implies a Novel Antiviral Strategy

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ACS Infectious Diseases 2024年第9期10卷 3304-3319页

作者： Štěrbová, Petra Wang, Chun-Hsiung Carillo, Kathleen J. D. Lou, Yuan-Chao Kato, Takayuki Namba, Keiichi Tzou, Der-Lii M. Chang, Wei-Hau Chemical Biology and Molecular Biophysics Program Taiwan International Graduate Program Academia Sinica College of Life Science National Tsing Hua University Institute of Chemistry Academia Sinica Biomedical Translation Research Center Academia Sinica Graduate School of Frontier Biosciences Osaka University Genomics Research Center Academia Sinica Institute of Physics Academia Sinica

Many viruses contain surface spikes or protrusions that are essential for virus entry. These surface structures can thereby be targeted by antiviral drugs to treat viral infections. Nervous necrosis virus (NNV), a simple nonenveloped virus in the genus of betanodavirus, infects fish and damages aquaculture worldwide. NNV has 60 conspicuous surface protrusions, each comprising three protrusion domains (P-domain) of its capsid protein. NNV uses protrusions to bind to common receptors of sialic acids on the host cell surface to initiate its entry via the endocytic pathway. However, structural alterations of NNV in response to acidic conditions encountered during this pathway remain unknown, while detailed interactions of protrusions with receptors are unclear. Here, we used cryo-EM to discover that Grouper NNV protrusions undergo low-pH-induced compaction and resting. NMR and molecular dynamics (MD) simulations were employed to probe the atomic details. A solution structure of the P-domain at pH 7.0 revealed a long flexible loop (amino acids 311-330) and a pocket outlined by this loop. Molecular docking analysis showed that the N-terminal moiety of sialic acid inserted into this pocket to interact with conserved residues inside. MD simulations demonstrated that part of this loop converted to a β-strand under acidic conditions, allowing for P-domain trimerization and compaction. Additionally, a low-pH-favored conformation is attained for the linker connecting the P-domain to the NNV shell, conferring resting protrusions. Our findings uncover novel pH-dependent conformational switching mechanisms underlying NNV protrusion dynamics potentially utilized for facilitating NNV entry, providing new structural insights into complex NNV-host interactions with the identification of putative druggable hotspots on the protrusion. © 2024 The Authors. Published by American Chemical Society.

关键词： conformational change disorder-to-order transition druggable pocket molecular movies pH sensing protein oligomerization solution structures

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Brain age estimation:premise,promise,and problems

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Neural Regeneration Research 2025年第8期20卷 2313-2314页

作者： Jarrad Perron Ji Hyun Ko Graduate Program in Biomedical Engineering Price Faculty of EngineeringUniversity of ManitobaWinnipegMBCanada PrairieNeuro Research Centre Kleysen Institute for Advanced MedicineHealth Sciences CentreWinnipegMBCanada Department of Human Anatomy and Cell Science Rady Faculty of Health SciencesUniversity of ManitobaWinnipegMBCanada

Premise:The com bined effects of modern healthcare practices which prolong lifespan and declining birthrates have created unprecedented changes in age demographics worldwide that are especially pronounced in Japan,South Korea,Europe,and North *** old age is the most significant predictor of dementia,global healthcare systems must rise to the challenge of providing care for those with neurodegenerative disorders.

关键词： estimation providing birth

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Harnessing novel engineered feeder cells expressing activating molecules for optimal expansion of NK cells with potent antitumor activity

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Cellular & Molecular Immunology 2022年第2期19卷 296-298页

作者： Bokyung Min Bitna Yang You-Sun Kim Gyeong Min Park HyunAh Kim Hyojin Kim Eun-Ji Kim Yu Kyeong Hwang Eui-Cheol Shin Sungyoo Cho BioMedical Science and Engineering Interdisciplinary Program KAISTDaejeonKorea Cell Therapy Research Center GC LabCellYonginKorea Laboratory of Immunology and Infectious Diseases Graduate School of Medical Science and EngineeringKAISTDaejeonKorea

Natural killer(NK)cells play an important role in the antitumor immune response as the major cytotoxic lymphocytes in the innate immune *** cells without any genetic modifications have been used for both autologous and allogeneic ***,many reports have suggested that allogeneic NK cells can reduce the recurrence of disease and improve survival through a graft-versus-leukemia effect without GVHD[1,2].

关键词： killer expressing optimal

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