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Exploring PETase-like enzyme from shotgun metagenome and co-expressing Colicin E7 in Escherichia coli for effective PET degradation

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Process Biochemistry 2024年 140卷 78-87页

作者： Effendi, Sefli Sri Wahyu Hu, Ruei-En Hsiang, Chuan-Chieh Ting, Wan-Wen Huang, Chao-Li Ng, I-Son Department of Chemical Engineering National Cheng Kung University Tainan701 Taiwan Institute of Tropical Plant Sciences and Microbiology National Cheng Kung University Tainan701 Taiwan Graduate Program in Translational Agricultural Sciences National Cheng Kung University and Academia Sinica Tainan701 Taiwan

Polyethylene terephthalate (PET) is the conventional plastic used for packaging but disposal of PET in the natural environment poses significant threats to the ecological system. In the biological process, the degradation of PET plastic using PET-degrading enzyme (PETase) has attracted scientific interest, thus increasing the exploration of PETase evolution. This study aims to exploit a new PETase from a greenhouse farm in Tainan, Taiwan. The in-silico approach of shotgun metagenomic analysis, enzyme structural prediction, molecular docking and simulations were applied, thus narrowing the cleft and identifying a potential PETase-like enzyme, denoted as F148. The heterologous expression of F148 in Escherichia coli strains was capable of breaking down PET into terephthalate monomer (TPA). Furthermore, the PET-degrading ability of F148 was optimized using the incorporation of MHETase and Colicin E7 via in vitro and in vivo digestion methods, respectively. The in vivo system served as a concise approach due to allowing simultaneous F148 secretion, thus achieving the highest TPA amount of 0.56 mg, equivalent to 11.2% weight loss of PET. This study not only contributes promising insights into the exploration of a distinctive gene but also successfully invents an efficient PETase using a one-step in vivo process in genetically modified E. coli. © 2024 Elsevier Ltd

关键词： Escherichia coli

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Classifying the Sustainability Profile of Clothing Customers

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Circular Economy and Sustainability 2022年第3期2卷 811-828页

作者： Garcia, Solimar de Alencar Nääs, Irenilza da Silva Lima, Nilsa Duarte Graduate Program in Production Engineering Universidade Paulista Rua Dr. Bacelar 1212 SP São Paulo Brazil

The textile sector faces reverse logistics, with activities that can foster a circular economy, such as recycling and reusing materials, a challenge. Industrial waste represents difficulties in all countries, and searching for a solution to the issues related to this subject is highly needed. In 2010 in Brazil, a National Solid Waste Policy (PNRS) was implemented, and after almost a decade, the textile industry is still far from adequate regulation. The consumer society and its unstoppable garbage production need action to reverse this situation. Education and conscious consumption are urgent, especially in developing and poorer countries. The present study’s objective was to propose a clothing consumer classification model based on recycling initiatives. The research was developed based on a questionnaire distributed by email and social media platforms. The questionnaire used the Google Platform to examine the garments consumers’ characteristics and profiles. The data mining approach was used to process the organized data, and random trees were built to express the models. The results describe the clothing consumers’ sustainability characteristics based on their gender, age range, school level, monthly income, purchasing and recycling habits, and commuting. Results indicate that consumer sustainability awareness is mainly related to the period they wear the clothes and how often they purchase them. Consumer behavioral habits such as recycling solid waste and commuting are also indicators of their sustainability degree related to buying and using clothes. © 2021, The Author(s), under exclusive licence to Springer Nature Switzerland AG.

关键词： Circular economy Classification algorithm Clothes chain Garment industry

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M-AxPPA: An Accurate and Energy-Efficient Modified Approximate Parallel Prefix Adder

M-AxPPA: An Accurate and Energy-Efficient Modified Approxima...

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IEEE International Conference on Electronics, Circuits and Systems (ICECS)

作者： Rodrigo Lopes Morgana da Rosa Eduardo da Costa Rafael Soares Graduate Program on Computing - Federal University of Pelotas (UFPel) Pelotas Brazil Graduate Program on Electronic Engineering and Computing - Catholic University of Pelotas (UCPel) Pelotas Brazil

ISBN: (数字)9798350377200

ISBN: (纸本)9798350377217

This work introduces a combined version of approximate adders (AxAs), joining approximate parallel prefix adders (AxPPA) with other AxAs from the literature (COPY, Truncation - TRUNC, and lower-part OR adder - LOA). We call this approach M-AxPPA (modified approximate parallel prefix adder) and investigate three specific versions: M-AxPPA-COPY, M-AxPPA-TRUNC, and M-AxPPA-LOA. We evaluate 315 different configurations, comparing our novel M-AxPPA to other AxAs proposed in the literature. We present pseudocode examples and investigate the accuracy quality, incorporating metrics like SSIM, NCC, MAE, MRE, and MRED. Additionally, we perform a trade-off analysis between accuracy quality and logic synthesis outcomes, underscoring the advantages of our M-AxPPA, notably the outstanding accuracy achieved by M-AxPPA-LOA (approaching 100%), with a substantial energy-saving.

关键词： Measurement Accuracy Energy efficiency Logic Adders Electronic circuits

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Compliance of Non-GSO Satellite with Radio Regulations Regarding to Interference with GSO Earth Stations. Case Study: Starlink and Telkom 3S 8

Compliance of Non-GSO Satellite with Radio Regulations Regar...

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8th International Conference on Information Technology, Information Systems and Electrical engineering, ICITISEE 2024

作者： Hidayati, Rizki Sutyarjoko, Meiditomo Wijanto, Heroe Graduate Program of Regulation and Management of Telecommunication School of Electrical Engineering Telkom University Bandung Indonesia Regulation and Management of Telecommunication School of Electrical Engineering Telkom University Bandung Indonesia The University Center of Excellence for Intelligent Sensing-IoT School of Electrical Engineering Telkom University Bandung Indonesia

ISBN: (纸本)9798350368970

This research aims an analyze of the interference analysis of the implementation of Telkom-3S and Starlink in Indonesia at downlink Ku-band Frequency based on the epfd calculation. This research conducts an analysis of the effect of avoidance angle on the interference caused by non-GSO satellites, it explores a concept concerning a victim GSO earth station. We use TLE to determine the number of Starlink that visible on the antenna ES of Telkom 3S. Based on the results of the calculations using an avoidance angle 0°- 80°, the higher of avoidance angle, the lower level of epfd and we also recognize that the epfd analyzed exceeds the interference limit suggested by ITU-R article 22 and based on the analysis of the epfd calculation using several avoidance angles, we recommend for Starlink to transmit the power level when the avoidance angle at 10° of ES Telkom 3S. © 2024 IEEE.

关键词： Satellite ground stations

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Assessment of Potential Tidal Power Sites in the Seto Inland Sea, Japan Using Multi-criteria Evaluation 1

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9th International Conference on Energy and Environment Research, ICEER 2022

作者： Aljber, Morhaf Sakanoue, Ginga Jeong, Jae-Soon Cabrera, Jonathan Salar Lee, Han Soo Transdisciplinary Science and Engineering Program Graduate School of Advanced Science and Engineering 1-5-1 Kagamiyama Hiroshima Higashi-Hiroshima739-8529 Japan The IDEC Institute Hiroshima University Hiroshima Japan

ISBN: (数字)9783031435591

ISBN: (纸本)9783031435584

This paper is an analytical study of the optimal locations for tidal power generation in the Seto Inland Sea (SIS) in Japan. Multiple criteria were considered and evaluated to determine the optimal locations in the SIS to deploy tidal turbines. The tidal current speed was considered in a position of 30 m depth below the sea surface, given that most of the current commercial turbines are functioning between 20 and 50 m underwater. Also, the distance from ports and the coastlines was included for the installation and maintenance of tidal devices. The distance from existing power generation plants is also considered for the outcome energy to be easily inserted into the grid. Further, renewable energy resources from wind and surface waves are also considered in the analysis for the potentiality of a combined power generation. The national parks and restricted areas were eliminated from the selection process. The assessment process was conducted using the analytic hierarchy process (AHP) in combination with GIS-based spatial analysis. The results illustrated that Kurushima Strait, Bisan Strait, Naruto Strait, and Akashi Strait are suitable locations for tidal power generation. Moreover, Bungo Channel and Kii Channel are highlighted as potential sites for wind-wave power generation sites. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2023.

关键词： Analytic hierarchy process

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AxMOD: VLSI Modular Reduction Design Exploring Approximate Arithmetic Units

AxMOD: VLSI Modular Reduction Design Exploring Approximate A...

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Symposium on Integrated Circuits and Systems Design (SBCCI)

作者： Lourenço Mulling Morgana M. A. Rosa Rafael Soares Eduardo Costa Graduate Program on Computing Federal University of Pelotas (UFPel) Pelotas Brazil Graduate Program on Electronic Engineering and Computing Catholic University of Pelotas (UCPel) Pelotas Brazil

ISBN: (数字)9798350391695

ISBN: (纸本)9798350391701

This work investigates the design of approximate arithmetic operator units used in the VLSI modular reduction (AxMOD) architecture. The AxMOD architecture herein proposed explores the following arithmetic operators: i) two approximate iterative-based fixed-point and Goldschmidt (GLD) dividers, ii) five approximate adders (AxA): COPY adder, error-tolerant adder (ETA), lower-part OR adder (LOA), truncation (Trunc), and approximate parallel prefix adder (AxPPA), and iii) five approximate multipliers of approximate radix-4 multiplier units (AxRMU), dynamic range unbiased multiplier (DRUM), rounding-based approximate (RoBA), truncated multiplier (Trunc), and the leading one-bit based approximate (LoBA). Our demonstration of AxMOD’s efficiency using approximate arithmetic operators from the Pareto-optimal front illustrates the area and power-quality trade-off. Our results reveal that in fixed-point arithmetic, AxRMU with K = 16 and Trunc adder with K = 8 deliver energy savings of up to 4.14× compared to the state-of-the-art.

关键词： Energy consumption Accuracy Energy conservation Power quality Very large scale integration Dynamic range Adders System analysis and design Fixed-point arithmetic

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The Influence of Digital Adoption, Absorptive Capacity and Risk Management Implementation on Organizational Resilience: An Empirical Study in The Indonesian Banking Industry

The Influence of Digital Adoption, Absorptive Capacity and R...

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Interdisciplinary Approaches in Technology and Management for Social Innovation (IATMSI), IEEE Conference on

作者： Boy Tjahyono Harjanto Prabowo Firdaus Alamsjah Mohammad Hamsal Research in Management BINUS Graduate Program BINUS Business School Jakarta Indonesia Industrial Engineering BINUS Graduate Program BINUS Business School Jakarta Indonesia

One of the primary difficulties of Indonesia's banking sector is to ensure that it continues to develop in business, is robust, and can foresee new risks while enhancing governance, risk, and compliance. This study will determine the effect of risk management implementation, absorptive capacity, and digital adoption on organizational resilience. The survey method is being used as a research method, with targeted respondents from senior executives, including C-suites, senior vice presidents, and vice presidents. The results show that digital adoption and absorptive capacity impact risk management implementation and organizational resilience despite the limited research available in the Indonesian banking sector. The study will contribute to understanding how enterprise resilience is affected by risk management. This research has presented an opportunity for both banking management and policymakers to formulate strategies aimed at bolstering organizational resilience within the banking sector. These strategies could aid in navigating unforeseen and disruptive business environments. Furthermore, this study stands to expand the application of concepts such as organizational resilience and dynamic capacity theory, particularly within the banking industry of developing nations.

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Experimental Evaluation of Thermal Performance of Oil-Immersed Power Converter 8

Experimental Evaluation of Thermal Performance of Oil-Immers...

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8th Southern Power Electronics Conference and the 17th Brazilian Power Electronics Conference, SPEC / COBEP 2023

作者： De Oliveira, João Henrique Turnbull, Jamie E. Venkataramanan, Giri Ludois, Daniel Universidade Federal de Minas Gerais Graduate Program in Electrical Engineering Belo Horizonte Brazil University of Wisconsin - Madison MadisonWI53703 United States

ISBN: (纸本)9798350321128

The quest for component size reduction raises concerns regarding device temperatures, particularly in power semi-conductor devices. In the pursuit of improved heat dissipation, several strategies can be used to optimize the heat transfer. This paper investigates the use of an oil immersed converter to enhance temperature performance in power converters. Prototypes were developed and tested in both air and Cargill FR3 oil. The case temperature of the IGBTs was monitored under various test scenarios involving power flow and switching frequency variations. The results consistently demonstrated lower IGBT temperatures in the oil-immersed converter across all test scenarios. In steady state, the temperature difference reached a significant 52% reduction compared to the ambient-air converter at the same operating point. These findings highlight the effectiveness of oil immersion in improving thermal performance in power converters. Hi-Pot tests demonstrate that when submerged in oil, high voltage levels can be applied to the board without experiencing voltage breakdown accompanied by sparks. © 2023 IEEE.

关键词： oil-immersed converter thermal stress voltage breakdown

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Deep Learning for Multivariate Prediction of Building Energy Performance of Residential Buildings

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Computers, Materials & Continua 2023年第6期75卷 5947-5964页

作者： Ibrahim Aliyu Tai-Won Um Sang-Joon Lee Chang Gyoon Lim Jinsul Kim Department of ICT Convergence System Engineering Chonnam National UniversityGwangju61186Korea Graduate School of Data Science Chonnam National UniversityGwangju61186Korea Interdisciplinary Program of Digital Future Convergence Service Chonnam National UniversityGwangju61186Korea Department of Computer Engineering Chonnam National UniversityYeosu59626Korea

In the quest to minimize energy waste,the energy performance of buildings(EPB)has been a focus because building appliances,such as heating,ventilation,and air conditioning,consume the highest ***,effective design and planning for estimating heating load(HL)and cooling load(CL)for energy saving have become *** this vein,efforts have been made to predict the HL and CL using a univariate ***,this approach necessitates two models for learning HL and CL,requiring more computational ***,the one-dimensional(1D)convolutional neural network(CNN)has gained popularity due to its nominal computa-tional complexity,high performance,and low-cost hardware *** this paper,we formulate the prediction as a multivariate regression problem in which the HL and CL are simultaneously predicted using the 1D *** the building shape characteristics,one kernel size is adopted to create the receptive fields of the 1D CNN to extract the feature maps,a dense layer to interpret the maps,and an output layer with two neurons to predict the two real-valued responses,HL and *** the 1D data are not affected by excessive parameters,the pooling layer is not applied in this ***,the use of pooling has been questioned by recent *** performance of the proposed model displays a comparative advantage over existing models in terms of the mean squared error(MSE).Thus,the proposed model is effective for EPB prediction because it reduces computational time and significantly lowers the MSE.

关键词： Artificial intelligence(AI) convolutional neural network(CNN) cooling load deep learning energy energy load energy building performance heating load prediction

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A Systematic Literature Review of Product Innovation Management for Food Manufacturing 2022

A Systematic Literature Review of Product Innovation Managem...

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3rd International Conference on Industrial engineering and Industrial Management, IEIM 2022

作者： Leesatapornwongsa, Fontip Thawesaengskulthai, Natcha Technopreneurship and Innovation Management Program Graduate School Chulalongkorn University Thailand Department of Industrial Engineering Faculty of Engineering Chulalongkorn University Thailand

ISBN: (纸本)9781450395694

Food innovations have become crucial for manufacturing's business performance;however, new food product still has low consumers adoption rate even with extensive of research and new knowledge. While the outcomes of innovation may appear random, but the development of invention is not. Key elements such as managerial strategy, internal collaboration, and resources are complicated to fully understand and manage. An organization should take a systematic and practical approach to comprehend the associated factors for innovation management. Innovation management has grown in popularity in study and practice. Despite the rising number of studies on management innovation, there is still a gap in research that combines this body of knowledge, specifically for product innovation for food manufacturing. Therefore, this study undertakes a systematic review of product innovation management for food manufacturing during 1980-2020 to better understand the management of food product innovation. The review concluded the six key success factors of product innovation management for food manufacturing, which are top management, organizational structure and culture, resources (human, finance, technology), process management, collaboration, and environment and market orientation. © 2022 ACM.

关键词： Product development

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