Cellular metabolism is dynamic, but quantifying non-steady metabolic fluxes by stable isotope tracers presents unique computational challenges. Here, we developed an efficient 13 C-tracer dynamic metabolic flux analys...
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Cellular metabolism is dynamic, but quantifying non-steady metabolic fluxes by stable isotope tracers presents unique computational challenges. Here, we developed an efficient 13 C-tracer dynamic metabolic flux analysis (13C-DMFA) framework for modeling central carbon fluxes that vary over time. We used B-splines to generalize the flux parameterization system and to improve the stability of the optimization algorithm. As proof of concept, we investigated how 3T3-L1 cultured adipocytes acutely metabolize glucose in response to insulin. Insulin rapidly stimulates glucose uptake, but intracellular pathways responded with differing speeds and magnitudes. Fluxes in lower glycolysis increased faster than those in upper glycolysis. Glycolysis fluxes rose disproportionally larger and faster than the tricarboxylic acid cycle, with lactate a primary glucose end product. The uncovered array of flux dynamics suggests that glucose catabolism is additionally regulated beyond uptake to help shunt glucose into appropriate pathways. This work demonstrates the value of using dynamic intracellular fluxes to understand metabolic function and pathway regulation.
Plagiarism in the education sector is as serious issues. In 2015 Indonesia ministry of research, technology and higher education stated to eradicate the plagiarism, and after 2 years the similar statement by Indonesia...
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Blended learning can be considered an approach that combines face-to-face and online periods in education through the integration of some technological resources, such as media centers. The present research sought to ...
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We discuss the effect of sequential error injection on information leakage under a network code. We formulate a network code for the single transmission setting and the multiple transmission setting. Under this formul...
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Quantum information science harnesses the principles of quantum mechanics to realize computational algorithms with complexities vastly intractable by current computer platforms. Typical applications range from quantum...
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Polyps are well-known cancer precursors identified by colonoscopy. However, variability in their size, location, and surface largely affect identification, localisation, and characterisation. Moreover, colonoscopic su...
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The smart city debate will still be the scope of business, policy-making and territorial planning in the following years. Based on that, the paper aims to propose an interdisciplinary conceptualization of smart territ...
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The smart city debate will still be the scope of business, policy-making and territorial planning in the following years. Based on that, the paper aims to propose an interdisciplinary conceptualization of smart territories. The goal is to promote the contrary movement of megacities creation and also consolidate the geographical aspect of smartness. The concept was formulated based on the elements of sustainable territorial development, strategic spatial planning and smart governance. In addition, taking hand of a parallel literature review, the study explores the potential of creating complementary cryptocurrencies as a strategy of territorial planning. This term was built based on already existing complementary currency systems in distributed ledger technologies scope. The idea provided presents a great potential for poverty alleviation by bringing together financial and digital inclusion.
Using the Subaru/FOCAS IFU capability, we examine the spatially resolved relationships between gas-phase metallicity, stellar mass, and star-formation rate surface densities (Σ★ and ΣSFR, respectively) in extremely...
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