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Unravelling biosynthesis and biodegradation potentials of microbial dark matters in hypersaline lakes

environmental Science and Ecotechnology 2024年第4期20卷 177-188页

作者： Zhiguang Qiu Yuanyuan Zhu Qing Zhang Xuejiao Qiao Rong Mu Zheng Xu Yan Yan Fan Wang Tong Zhang Wei-Qin Zhuang Ke Yu School of Environment and Energy Peking University Shenzhen Graduate SchoolShenzhen518055China AI for Science(AI4S)-Preferred Program Peking UniversityShenzhen518055China Southern University of Sciences and Technology Yantian Hospital Shenzhen518081China Institute of Biomedicine and Biotechnology Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen518055China State Key Laboratory of Isotope Geochemistry CAS Center for Excellence in Deep Earth ScienceGuangzhou Institute of GeochemistryChinese Academy of SciencesGuangzhou510640China School of Atmospheric Sciences Sun Yat-sen UniversitySouthern Marine Science and Engineering Guangdong Laboratory(Zhuhai)Zhuhai519082China Department of Civil Engineering University of Hong Kong999077Hong KongChina Department of Civil and Environmental Engineering Faculty of EngineeringUniversity of AucklandNew Zealand

Biosynthesis and biodegradation of microorganisms critically underpin the development of biotechnology,new drugs and therapies,and environmental ***,most uncultured microbial species along with their metabolic capacities in extreme environments,remain *** we unravel the metabolic potential of microbial dark matters(MDMs)in four deep-inland hypersaline lakes in Xinjiang,*** metagenomic binning,we uncovered a rich diversity of 3030 metagenomeassembled genomes(MAGs)across 82 phyla,revealing a substantial portion,2363 MAGs,as previously unclassified at the genus *** unknown MAGs displayed unique distribution patterns across different lakes,indicating a strong correlation with varied physicochemical *** analysis revealed an extensive array of 9635 biosynthesis gene clusters(BGCs),with a remarkable 9403 being novel,suggesting untapped biotechnological ***,some MAGs from potentially new phyla exhibited a high density of these *** biosynthesis,our study also identified novel biodegradation pathways,including dehalogenation,anaerobic ammonium oxidation(Anammox),and degradation of polycyclic aromatic hydrocarbons(PAHs)and plastics,in previously unknown microbial *** findings significantly enrich our understanding of biosynthesis and biodegradation processes and open new avenues for biotechnological innovation,emphasizing the untapped potential of microbial diversity in hypersaline environments.

关键词： Biosynthesis Biodegradation Hypersaline lakes Metagenome-assembled genomes Microbial dark matters

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Biological methane production coupled with sulfur oxidation in a microbial electrosynthesis system without organic substrates

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Journal of environmental Sciences 2022年第6期34卷 68-78页

作者： Ha T.T.Dinh Hiromi Kambara Shuji Matsushita Yoshiteru Aoi Tomonori Kindaichi Noriatsu Ozaki Akiyoshi Ohashi Department of Civil and Environmental Engineering Graduate School of Advanced Science and EngineeringHiroshima University1-4-1KagamiyamaHigashihiroshimaHiroshima 739-8527Japan Faculty of Environment Ho Chi Minh City University of Natural Resources and Environment236 Le Van Sy1 WardTan Binh DistrictHo Chi Minh CityViet Nam Agricultural Technology Research Center Hiroshima Prefectural Technology Research Institute6869HaraHachihonmatsuHigashihiroshimaHiroshima 739-0151Japan Program of Biotechnology Graduate School of Integrated Sciences for LifeHiroshima University1-3-1KagamiyamaHigashihiroshimaHiroshima 739-8530Japan

Methane is produced in a microbial electrosynthesis system(MES) without organic substrates. However, a relatively high applied voltage is required for the bioelectrical *** this study, we demonstrated that electrotrophic methane production at the biocathode was achieved even at a very low voltage of 0.1 V in an MES, in which abiotic HS-oxidized to SO_(4)^(2-) at the anodic carbon-cloth surface coated with platinum powder. In addition, microbial community analysis revealed the most probable pathway for methane production from electrons. First, electrotrophic H_(2) was produced by syntrophic bacteria, such as Syntrophorhabdus, Syntrophobacter, Syntrophus, Leptolinea, and Aminicenantales, with the direct acceptance of electrons at the biocathode. Subsequently, most of the produced H_(2) was converted to acetate by homoacetogens, such as Clostridium and Spirochaeta 2. In conclusion,the majority of the methane was indirectly produced by a large population of acetoclastic methanogens, namely Methanosaeta, via acetate. Further, hydrogenotrophic methanogens,including Methanobacterium and Methanolinea, produced methane via H_(2).

关键词： Bioelectricity Homoacetogens Methane production Microbial electrosynthesis system Sulfur oxidation

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Spatially differentiated life cycle assessment of Thailand's transport: The implications from country-specific factors and alternative technologies

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Sustainable Production and Consumption 2025年 57卷 319-340页

作者： Vitoon Chotanapund Shabbir H. Gheewala Vladimir Strezov Nazmul Huda Jitti Mungkalasiri Trakarn Prapaspongsa Graduate Program in Environmental and Water Resources Engineering Department of Civil and Environmental Engineering Faculty of Engineering Mahidol University Nakhon Pathom Thailand School of Natural Sciences Faculty of Science and Engineering Macquarie University NSW 2109 Australia The Joint Graduate School of Energy and Environment (JGSEE) King Mongkut's University of Technology Thonburi Bangkok Thailand Center of Excellence on Energy Technology and Environment Ministry of Higher Education Science Research and Innovation Ministry of Education Bangkok Thailand School of Engineering Faculty of Science and Engineering Macquarie University NSW 2109 Australia Technology and Informatics Institute for Sustainability (TIIS) National Metal and Materials Technology Center (MTEC) National Science and Technology Development Agency (NSTDA) Pathum Thani Thailand

This study proposed country-specific characterisation factors (CFs) for Thailand by modifying the Thai Spatially Differentiated Life Cycle Impact Assessment (ThaiSD) method and introducing monetary conversion factors to express environmental impacts in Thai Baht. Five impact categories were fully parameterised, including fine particulate matter formation (PMF), human toxicity (both cancer and non-cancer) (HT c and HT nc ), freshwater ecotoxicity (FET), and water scarcity (WS). Other spatialised CFs were selected from regionalisation models in ReCiPe2016 and IMPACT World+. When comparing the use of country-specific CFs with global average, the impact scores for PMF, HT c , HT nc , FET, photochemical ozone formation and terrestrial acidification demonstrated notably different levels, ranging from ±25 to ±50 %. The developed method was then applied to assess environmental impacts and costs of Thailand's transport sector, encompassing freight and passenger transport across roadway, railway, waterway, and aviation. Climate change (CC) was significantly attributed to human health and ecosystem quality impacts, while the major contributor to resource scarcity impact was fossil resource scarcity (FS). In some scenarios, non-exhaust emissions, particularly from freight trucks, accounted for 40–60 % of the total PMF impacts. environmental costs of freight and passenger transport in Thailand were 0.08–3.64 Thai Baht per tonne-kilometre and 0.01–0.81 Thai Baht per passenger-kilometre, respectively. Among transport modes, trains were found to be the most environmentally favourable option for both passenger and freight transport, while aviation had the highest environmental impact for freight transport. In contrast, passenger aviation had a comparable environmental burden to passenger cars due to optimised occupancy rates. Despite the efficiency of modern internal combustion engine vehicles (ICEVs), particularly Euro 5 and 6, employing blended biodiesel was less effective in comp

关键词： Life cycle assessment Thai Spatially Differentiated Life Cycle Impact Assessment Freight transport Passenger transport Transport technologies

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Design of a Central Tool for Sustainability Evaluation and Weighting to Decision-Making Support of Contaminated Soils Remediation

SSRN

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SSRN 2023年

作者： Braun, Adeli Beatriz Visentin, Caroline Thomé, Antônio Graduate Program in Civil and Environmental Engineering University of Passo Fundo BR 285 São José Rio Grande do Sul Passo Fundo99052-900 Brazil

Sustainable remediation remains unstandardized and ambiguously regulated, thereby limiting its adoption in the management of contaminated areas. Although a considerable number of tools are available for this purpose, numerous shortcomings continue to be detected, especially with regard to the integration of a complete assessment of impact and sustainability into a single framework. In view of these problems, this study developed and validated an integrated tool for assessing the sustainability of remediation techniques for contaminated soils. To support the sustainability analysis, we prepared impact assessment matrices, which list components and criteria for obtaining integrated impact scores for each sustainability dimension/pillar (i.e., social, economic, and environmental), factor, and component. These impact scores were incorporated and fitted into a mathematical model used to ascertain the sustainability of the techniques. The tool was subsequently validated by comparing and analyzing the sustainability with which five techniques: phytoremediation, electrokinetics and excavation/disposal (Case Study I), nanoremediation and soil washing (Case Study II). The techniques’ probabilities of sustainability followed the order presented in the preceding statement. The determination of sustainability was supported by the direct interaction between the effects derived under each dimension and technique. These findings led to the conclusion that the proposed tool prioritizes the basic principles of sustainability, which call for harmony among the three pillars, and that it is a favorable instrument for the evaluation and selection of a sustainable remediation technique. © 2023, The Authors. All rights reserved.

关键词： Sustainable development

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Design of a Central Tool for Sustainability Evaluation and Weighting to Decision-Making Support of Contaminated Soils Remediation

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SSRN 2023年

关键词： Sustainable development

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Global variations in critical drought thresholds that impact vegetation

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National Science Review 2023年第5期10卷 180-193页

作者： Xiangyi Li Shilong Piao Chris Huntingford Josep Pe?uelas Hui Yang Hao Xu Anping Chen Pierre Friedlingstein Trevor F.Keenan Stephen Sitch Xuhui Wang Jakob Zscheischler Miguel D.Mahecha Sino-French Institute for Earth System Science College of Urban and Environmental SciencesPeking University Key Laboratory of Alpine Ecology and Biodiversity Institute of Tibetan Plateau ResearchCenter for Excellence in Tibetan Earth ScienceChinese Academy of Sciences UK Centre for Ecology and Hydrology CSIC Global Ecology Unit CREAF-CSIC-UAB CREAF Cerdanyola de Vallès Department of Biogeochemical Integration Max Planck Institute for Biogeochemistry Department of Biology Colorado State University Graduate Degree Program in Ecology Colorado State University Department of Mathematics College of Engineering Mathematics and Physical SciencesUniversity of Exeter Laboratoire de Météorologie Dynamique Institut Pierre-Simon LaplaceCNRS-ENS-UPMC-XDépartement de GeósciencesEcole Normale Superieure Earth and Environmental Sciences Area Lawrence Berkeley National Laboratory Department of Environmental Science Policy&ManagementUniversity of California Berkeley Department of Geography College of Life and Environmental SciencesUniversity of Exeter Department of Computational Hydrosystems Helmholtz Centre for Environmental Research—Ufz Remote Sensing Centre for Earth System Research Leipzig University

Identifying the thresholds of drought that,if crossed,suppress vegetation functioning is vital for accurate quantification of how land ecosystems respond to climate variability and *** present a globally applicable framework to identify drought thresholds for vegetation responses to different levels of known soil-moisture deficits using four remotely sensed vegetation proxies spanning *** thresholds identified represent critical inflection points for changing vegetation responses from highly resistant to highly vulnerable in response to drought stress,and as a warning signal for substantial vegetation *** thresholds varied geographically,with much lower percentiles of soil-moisture anomalies in vegetated areas covered by more forests,corresponding to a comparably stronger capacity to mitigate soil water deficit stress in forested ***,those lower thresholds are detected in more humid ***-of-the-art land models,however,overestimated thresholds of soil moisture(*** drought impacts),especially in more humid areas with higher forest covers and arid areas with few forest *** on climate model projections,we predict that the risk of vegetation damage will increase by the end of the twenty-first century in some hotspots like East Asia,Europe,Amazon,southern Australia and eastern and southern *** data-based results will inform projections on future drought impacts on terrestrial ecosystems and provide an effective tool for drought management.

关键词： drought threshold inflection points vegetation response soil moisture drought impacts

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Health risk assessment of soil contamination with heavy metals in a child care center co-located in vicinity to small scale industrial area: Case study of Thailand

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Case Studies in Chemical and environmental engineering 2024年 9卷

作者： Simatupang, Cathleen Ariella Santhaweesuk, Kanitthika Strezov, Vladimir Pongkiatkul, Prapat Boontanon, Narin Jindal, Ranjna Boontanon, Suwanna Kitpati Graduate Program in Environmental and Water Resources Engineering Department of Civil and Environmental Engineering Faculty of Engineering Mahidol University Nakhon Pathom 73170 Thailand School of Natural Sciences Faculty of Science and Engineering Macquarie University 2109 NSW Australia Department of Environmental Engineering King Mongkut's University of Technology Thonburi Bangkok 10800 Thailand Faculty of Environment and Resource Studies Mahidol University Nakhon Pathom 73170 Thailand Environmental Engineering and Management Program Department of Energy Environment and Climate Change School of Environment and Resources Development Asian Institute of Technology Pathumthani 12120 Thailand Graduate School of Global Environmental Studies Kyoto University Yoshida Sakyo-Ku Kyoto 606-8501 Japan

environmental impacts of small scale industries are still not well investigated, especially for estates where multiple small scale industries are clustered causing cumulative effects potentially impacting most vulnerable groups. This study aimed to investigate the concentrations of heavy metals (Mn, Fe, Ni, Cu, Pb, Al, Zn, and Cr) and the metalloid (As) in surface soils near the intensely active small-scale industrial area in Thailand, assess the contamination levels in the co-located child care center, and estimate potential human health risks from exposure to the heavy metals. Surface soil heavy metal(loid) elements were evaluated across a background area, area near main roads, a small-scale industrial area, and a child care centre. The concentrations of Al were found be the highest at all investigation sites, with the most elevated concentrations observed in the small-scale industrial area. The concentration of Pb in the industrial area reached 1176 mg/kg, exceeding the national standard and acceptable limit. Moreover, the contamination level for Cu, Pb, and Zn in the industrial area imply a considerable risk with enrichment factor EF > 40, and geo-accumulation index Igeo ≥5. Heavy metal levels (Ni, Cu, Pb) in the soil near the road and the child care center were similar, suggesting shared contamination sources. Conversely, Al, Zn, As, and Cr in the child care center had slightly higher concentrations than near the road but lower than the small-scale industrial area, indicating potential industrial influence on metal levels within the child care center. The principal component analysis showed Mn and Fe distributed in the background, while As, Pb, Ni, Cu, Al, Zn, and Cr accumulated in the small-scale industrial area. The findings indicate that small-scale industrial activities contribute to elevated levels of heavy metals in the soil, particularly within the child care center, posing significant ingestion risks, especially for children (HI > 1). Implementing conta

关键词： Children Contamination sources environmental impact PCA Surface soil

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Determination of Dielectric Properties of Plaster Blocks for Sealing Masonry Using Frequency Scanning Methods

SSRN

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SSRN 2023年

作者： Praxedes, Maria Elizabeth Teixeira Santana Duarte, José G. de Medeiros Gurgel Pinto, Erica Natasche Cabral, Kleber Cavalcanti Silva Neto, Valdemir P. D’Assunção, Adaildo G. Graduate Program in Civil and Environmental Engineering Federal University of Rio Grande do Norte RN Natal Brazil Department of Communication Engineering Federal University of Rio Grande do Norte RN Natal Brazil

With the increasing use of traditional and new models of wireless communication systems, the study and determination of the electrical characteristics of materials used in civil construction is an important topic to establish an understanding of how the radio frequency signal behaves inside built environments. This study presents an extensive process of characterization of electrical parameters of plaster blocks used in the construction of walls. Analyses are made in the frequency range from 0.7 to 5.2 GHz, which has been used for several wireless communication standards. To carry out the electrical characterization of dielectric materials, a non-invasive methodology is proposed based on an innovative combination of the Nicolson–Ross–Weir Method (NRW) and the Ray Tracing Method. Through the proposed methodology and an extensive campaign of measurements using frequency scanning equipment, calculated and experimental data of Shielding Effectiveness (SE), complex relative electrical permittivity, loss tangent, attenuation coefficient and conductivity were obtained for a wide range of frequency, considering different samples of (gypsum?) plaster blocks. The obtained results are compared to those available in the related literature, confirming the accuracy of the proposed analysis. © 2023, The Authors. All rights reserved.

关键词： Ray tracing

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Study of Machine Learning Techniques for Damage Identification in a Beam 10th

Study of Machine Learning Techniques for Damage Identifica...

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10th International Conference on Wave Mechanics and Vibrations, WMVC 2022

作者： da Silva Coelho, Jefferson de Sousa, Amanda Aryda Silva Rodrigues Machado, Marcela Rodrigues Dutkiewicz, Maciej Department of Mechanical Engineering University of Brasília Campus Universitário Darcy Ribeiro Faculdade de Tecnologia - Asa Norte DF Brasília Brazil Post-Graduate Program - Integrity of Engineering Materials University of Brasília Campus Universitário Darcy Ribeiro Faculdade de Tecnologia - Asa Norte DF Brasília70910-900 Brazil Faculty of Civil Environmental Engineering and Architecture University of Science and Technology Bydgoszcz85-796 Poland

ISBN: (纸本)9783031157578

Damage is defined as any change to the material, geometry or boundary condition that can modify the structure’s properties or response. In the past, damage identification was performed through periodical inspection, non-destructive testing/non-destructive evaluation, or visual observation. Structural health monitoring (SHM) has emerged to transition from offline damage identification to near real-time and online damage assessment. Hence, SHM is a damage detection strategy to monitor a structure for a period using a series of continuous measurement devices, which then collect the system’s characteristics and subsequently perform statistical analysis to assess the current circumstances and health of the structure. One of the first steps for the study of SHM is damage detection followed by monitoring. Machine Learning (ML) techniques can be used to develop viable algorithms to make potential predictions. ML algorithms are therefore providing the tools needed to enhance the capabilities of SHM systems and provide intelligent solutions to past challenges. Preliminary studies have reviewed that the extension of ML into SHM has dramatically increased the system’s capabilities, providing innovative solutions to different research challenges. This work conducts a study of the use of ML to identify damage in a beam and discuss the challenge of usage, performance and implementation of each technique. © 2023, The Author(s), under exclusive license to Springer Nature Switzerland AG.

关键词： Decision trees

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Importance of surface charge of soot nanoparticles in determining inhalation toxicity in mice

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environmental Science and Pollution Research 2023年第7期30卷 18985-18997页

作者： Hsiao, Ta-Chih Han, Chia-Li Yang, Tzu-Ting Lee, Yueh-Lun Shen, Yu-Fang Jheng, Yu-Teng Lee, Chii-Hong Chang, Jer-Hwa Chung, Kian Fan Kuo, Han-Pin Chuang, Hsiao-Chi Graduate Institute of Environmental Engineering National Taiwan University Taipei Taiwan Master Program in Clinical Genomics and Proteomics College of Pharmacy Taipei Medical University Taipei Taiwan Department of Environmental Engineering and Health Yuanpei University of Medical Technology Hsin Chu City Taiwan Department of Microbiology and Immunology School of Medicine College of Medicine Taipei Medical University Taipei Taiwan Graduate Institute of Environmental Engineering National Center University Tauyoun Taiwan Genome and Systems Biology Degree Program Academia Sinica and National Taiwan University Taipei Taiwan Department of Pathology Taipei City Hospital Heping Fuyou Branch Taipei Taiwan School of Respiratory Therapy College of Medicine Taipei Medical University Taipei Taiwan Division of Pulmonary Medicine Department of Internal Medicine Wan Fang Hospital Taipei Medical University Taipei Taiwan National Heart and Lung Institute Imperial College London London United Kingdom Division of Pulmonary Medicine Department of Internal Medicine School of Medicine College of Medicine Taipei Medical University Taipei Taiwan Division of Pulmonary Medicine Department of Internal Medicine Shuang Ho Hospital Taipei Medical University New Taipei City Taiwan Cell Physiology and Molecular Image Research Center Wan Fang Hospital Taipei Medical University Taipei Taiwan

Physicochemical properties of nanoparticles are important in regulating nanoparticle toxicity;however, the contribution of nanoparticle charge remains unclear. The objective of this study was to investigate the pulmonary effects of inhalation of charged soot nanoparticles. We established a stably charged nanoparticle generation system for whole-body exposure in BALB/c mice, which produced positively charged, negatively charged, and neutral soot nanoparticles in a wide range of concentrations. After a 7-day exposure, pulmonary toxicity was assessed, together with proteomics analysis. The charged soot nanoparticles on average carried 1.17–1.35 electric charges, and the sizes for nanoparticles under different charging conditions were all fixed at 69 ~ 72 nm. We observed that charged soot nanoparticles induced cytotoxic LDH and increased lung permeability, with the release of 8-isoprostane and caspase-3 and systemic IL-6 in mice, especially for positively charged soot nanoparticles. Next, we observed that positive-charged soot nanoparticles upregulated Eif2, Eif4, sirtuin, mammalian target of rapamycin (mTOR), peroxisome proliferator-activated receptors (PPAR), and HIPPO-related signaling pathways in the lungs compared with negatively charged soot nanoparticles. HIF1α, sirt1, E-cadherin, and Yap were increased in mice’s lungs by positively charged soot nanoparticle exposure. In conclusion, carbonaceous nanoparticles carrying electric ions, especially positive-charged, are particularly toxic when inhaled and should be of concern in terms of pulmonary health protection. © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

关键词： Nanoparticles

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